GOtcha is a system to predict the function of a protein and assign confidence to the prediction.
A manuscript describing the method has been published in BMC Bioinformatics.
You can try out the GOtcha web server, but please do not write scripts to submit large numbers of sequences to this
server. Instead, contact David Martin to obtain a copy that you can run on your own computer systems.
GOtcha was conceived and developed by David Martin and his announcement to the go-friends mailing list is shown below:
18th November 2004
I am pleased to finally announce the GOtcha tool to the GO community at large. GOtcha is a tool for
assigning GO terms based on sequence similarity searches to well annotated genomes.
So why is GOtcha different to what is already out there and why should you be using it?
- GOtcha uses a method where
it combines information from many search hits, up to and including E-values that are normally discarded. This gives much better sensitivity than other methods.
- GOtcha provides a score for each individual
term, not just the leaf term or branch. This allows the discrimination between confident assignments that one would find at a more general level and the more specific terms that one would have lower confidence
- The scores GOtcha provides are calibrated to give a real estimate of correctness. This is expressed as a percentage, giving a result that non-experts are comfortable in interpreting.
provides graphical output that gives an overview of the confidence in, or potential alternatives for, particular GO term assignments.
The paper describing GOtcha has just been accepted by BMC Bioinformatics ( http://www.biomedcentral.com/1471-2105/5/178/abstract) and a web server is available at http://www.compbio.dundee.ac.uk/Software/GOtcha/gotcha.html for you to try.
Please do not write scripts against this server. I am currently getting the code packaged for distribution (it is already working in several collaborators laboratories) so if you have a desire to run large sets of sequences through GOtcha, please email me.
I hope you find GOtcha useful. All feedback will be gratefully received.