Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 G 3 -2 -1 -1 -1 -1 -1 3 -2 -3 -3 -1 -2 -3 -1 3 0 -3 -2 -2 41 0 0 0 0 0 0 24 0 0 0 0 0 0 0 35 0 0 0 0 0.44 0.11 2 R -2 7 -1 -2 -4 0 -1 -3 -1 -4 -3 2 -2 -4 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.21 3 L -1 -2 -3 -3 1 -1 -2 -3 -2 0 3 1 6 -1 -3 -1 -1 -2 0 0 4 0 0 0 4 0 0 0 0 0 30 11 39 0 0 6 2 0 4 0 0.35 0.22 4 F -3 -4 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 -1 8 -5 -3 -3 0 3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.22 0.22 5 K -1 2 -1 -1 -4 1 1 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 7 0 0 0 4 2 0 0 0 0 87 0 0 0 0 0 0 0 0 0.80 0.22 6 I -1 -4 -4 -4 -2 -3 -4 -4 -4 4 1 -3 0 -1 -3 -3 -1 -4 -2 4 0 0 0 0 0 0 0 0 0 46 0 0 0 0 0 0 0 0 0 54 0.64 0.22 7 T -1 -2 -1 -2 -2 -1 -2 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 94 0 0 0 0.80 0.22 8 A 4 -2 -3 -3 -1 -2 -2 -1 -2 -1 -2 -2 -1 0 -2 0 -1 -2 3 1 71 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 14 11 0.46 0.22 9 C -1 -4 -4 -4 9 -3 -4 -4 -4 0 -1 -4 -1 -3 -3 -2 -1 -3 -3 2 0 0 0 0 69 0 0 0 0 0 2 0 0 0 0 0 0 0 0 28 1.17 0.22 10 V -1 -3 -4 -4 -2 -1 -3 -4 -3 3 2 -3 3 0 -3 -3 -1 -3 -2 4 0 0 0 0 0 4 0 0 0 18 20 0 11 4 0 0 0 0 0 43 0.44 0.22 11 P -2 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -5 8 -1 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.31 0.22 12 S 1 -2 0 -1 2 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 5 2 -4 -2 -2 11 0 0 0 5 0 0 0 0 0 0 0 0 0 0 77 7 0 0 0 0.60 0.22 13 Q -2 0 -1 -1 -4 5 3 -3 -1 -3 -1 0 -1 -4 3 -1 0 -3 -2 -3 0 0 0 0 0 53 19 0 0 0 8 0 0 0 16 0 4 0 0 0 0.64 0.22 14 T 0 0 -1 -1 -2 -1 1 -2 -2 -1 -2 0 -2 -3 -2 3 4 -3 -2 0 0 3 0 0 0 0 13 0 0 0 0 4 0 0 0 34 35 0 0 11 0.37 0.22 15 R -2 6 1 -2 -4 0 -1 -3 -1 -4 -3 3 -2 -4 -3 -1 -2 -4 -3 -3 0 77 7 0 0 0 0 0 0 0 0 16 0 0 0 0 0 0 0 0 0.91 0.22 16 I -2 -3 -2 1 -2 -3 -3 -2 -3 4 1 -3 0 -1 -3 -2 1 -3 -2 2 0 0 0 11 0 0 0 4 0 51 8 0 0 0 0 0 10 0 0 16 0.36 0.22 17 R -2 6 -1 0 -4 2 0 -3 -1 -3 -3 1 -2 1 -3 -1 -2 -3 -1 -3 0 69 0 6 0 14 0 0 0 0 0 0 0 12 0 0 0 0 0 0 0.72 0.22 18 T 0 -2 -1 -2 -2 -1 -1 -2 -2 -2 -2 -1 -1 -3 -2 2 6 -3 -2 -1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 17 80 0 0 0 0.70 0.22 19 Q -1 0 -1 -2 -3 6 0 1 -1 -2 -2 0 -1 0 -2 -1 -1 -2 0 0 0 0 0 0 0 68 0 13 0 0 0 0 0 4 0 0 0 0 4 11 0.56 0.22 20 R 0 6 -1 -2 -4 0 -1 -3 -1 -4 -3 1 -2 -4 -3 -1 -2 -3 -2 -3 11 89 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.96 0.22 21 E -2 -1 -1 1 -4 1 6 -3 -1 -3 -1 0 -2 -3 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 89 0 0 0 11 0 0 0 0 0 0 0 0 0 0.85 0.22 22 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 1 2 -4 -3 -2 -2 -1 0 0 0 0 0 0 0 0 0 0 3 86 0 0 11 0 0 0 0 0 0 0.69 0.22 23 Q -1 0 -1 -1 -3 7 1 -3 0 -3 -3 1 -1 -4 -2 -1 1 -3 -2 -3 0 0 0 0 0 89 0 0 0 0 0 0 0 0 0 0 11 0 0 0 0.90 0.22 24 N -2 -1 7 1 -3 -1 -1 -1 0 -4 -4 -1 -3 -4 -3 0 -1 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.14 0.22 25 T -1 -2 -1 -2 -2 -2 -2 -3 -3 0 -1 -2 -1 -3 -2 0 6 -3 -2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 82 0 0 18 0.66 0.22 26 Y -1 -3 -3 -4 -3 -3 -3 -4 0 -2 -1 -3 -1 5 -4 -2 -2 1 7 -2 6 0 0 0 0 0 0 0 0 0 0 0 0 26 0 0 0 0 68 0 1.03 0.22 27 F -2 -3 -3 -4 -3 -3 -3 -4 -2 -1 -1 -3 -1 6 -4 -2 1 0 4 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 67 0 0 18 0 11 4 0.78 0.22 28 T -1 -2 -1 -2 -2 -1 -2 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.84 0.22 29 K -1 2 -1 -1 -4 1 0 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.88 0.22 30 L -2 -3 -4 -4 1 -3 -4 -4 -2 1 4 -3 2 0 -4 -3 -2 6 2 0 0 0 0 0 4 0 0 0 0 4 54 0 6 0 0 0 0 16 11 4 0.62 0.22 31 V -1 -3 -4 -4 -2 -3 -3 -4 -4 2 0 -3 0 -2 -3 -2 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 97 0.70 0.22 32 P -1 -3 0 1 -3 -2 -1 -2 -2 -4 -4 -2 -3 -4 7 0 -1 -5 -4 -3 0 0 4 11 0 0 0 0 0 0 0 0 0 0 76 9 0 0 0 0 1.60 0.22 33 Y -3 -3 -3 -4 -3 -3 -3 -4 0 -2 -1 -3 -1 5 -4 -3 -3 1 7 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 33 0 0 0 0 67 0 1.12 0.22 34 E -1 -2 0 5 -3 0 3 -2 0 -4 -4 -1 -3 -4 -2 2 0 -4 -3 -3 0 0 0 41 0 0 28 0 3 0 0 0 0 0 0 23 6 0 0 0 0.63 0.22 35 N 0 -1 6 0 -3 -1 -1 -1 -1 -4 -4 -1 -3 -4 -2 3 0 -4 -3 -3 3 0 61 0 0 0 0 0 0 0 0 0 0 0 0 36 0 0 0 0 0.74 0.22 36 W -3 -3 -4 -5 -3 -3 -4 -3 -3 -3 -2 -4 -2 0 -4 -2 -3 12 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 97 0 0 2.74 0.22 37 F -3 -3 -4 -4 -3 -4 -4 -4 -1 -1 0 -4 -1 7 -4 -3 -3 1 5 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 82 0 0 0 0 18 0 1.11 0.22 38 R 3 4 -1 -2 -2 -1 -1 -1 -2 -3 -3 1 -2 -3 -2 1 0 -3 -3 -2 48 32 2 0 0 0 0 2 0 0 0 5 0 0 0 6 5 0 0 0 0.43 0.22 39 E -2 -1 -1 1 -5 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 1.07 0.22 40 Q -1 0 -1 -1 -4 7 1 -3 0 -4 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.05 0.22 41 Q -1 0 -1 -1 -4 7 1 -3 0 -4 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.05 0.22 42 R -2 7 -1 -2 -4 0 -1 -3 -1 -4 -3 3 -2 -4 -3 -1 -2 -4 -2 -3 0 89 0 0 0 0 0 0 0 0 0 11 0 0 0 0 0 0 0 0 1.05 0.22 43 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 1 -4 1 -1 -4 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.78 0.22 44 Q -2 0 -1 -2 -3 5 0 -3 -1 -1 0 0 5 -2 -2 -1 0 -3 -2 -1 0 0 0 0 0 58 0 0 0 0 0 0 39 0 0 0 3 0 0 0 0.63 0.22 45 K -1 2 -1 -1 -4 1 0 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.88 0.22 46 M 2 -2 -2 -3 -2 2 -1 -2 -2 0 1 -1 6 -2 -2 -1 -1 -3 -2 -1 26 0 0 0 0 16 0 0 0 0 3 0 53 0 0 2 0 0 0 0 0.44 0.22 47 G 0 -3 -1 -2 -3 -3 -3 6 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.38 0.22 48 G 0 -3 -1 -2 -3 -3 -3 6 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.38 0.22 49 K -1 3 -1 -2 -4 1 0 -2 -1 -3 -3 5 -2 -4 -2 0 0 -4 -3 -3 0 20 0 0 0 0 0 0 0 0 0 71 0 0 0 5 4 0 0 0 0.71 0.22 50 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 1 -3 1 -1 -4 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 96 0 0 0 0 0 0 0 0 0 4 0.77 0.22 51 V -2 -3 -4 -4 -2 -3 -3 -4 -4 3 3 -3 2 1 -3 -3 0 -3 -1 3 0 0 0 0 0 0 0 0 0 23 36 0 4 8 0 0 6 0 0 24 0.44 0.23 52 K -1 2 -1 -1 -4 1 0 -2 -1 -4 -3 6 -2 -4 -2 0 -1 -4 -3 -3 0 2 0 0 0 0 0 0 0 0 0 91 0 0 0 6 0 0 0 0 0.80 0.23 53 V -1 -3 -4 -4 -2 -3 -3 -4 -4 2 0 -3 0 -2 -3 -2 -1 -4 -2 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.72 0.23 54 E -2 0 -1 1 -4 2 5 -3 1 -4 -4 3 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 4 74 0 3 0 0 20 0 0 0 0 0 0 0 0 0.82 0.23 55 L -2 -3 -4 -5 -2 -3 -4 -5 -4 1 5 -3 1 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.79 0.23 56 A 4 -2 -2 -3 3 -2 -2 -1 -3 -1 -2 -2 -1 0 -2 1 1 -3 -2 1 62 0 0 0 7 0 0 0 0 0 0 0 0 5 0 4 10 0 0 11 0.41 0.23 57 T 1 -2 2 -1 -2 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 2 5 -3 -2 0 9 0 10 0 0 0 0 0 0 2 0 0 0 0 0 14 61 0 0 4 0.51 0.23 58 G 0 -3 -1 -2 -3 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.38 0.23 59 K -1 4 -1 -2 -3 0 -1 1 -2 -2 -3 4 -2 -3 -2 0 -1 -4 -3 0 0 31 0 0 0 0 0 12 0 2 0 41 0 0 0 4 0 0 0 10 0.45 0.23 60 Q -1 2 -1 -2 -3 5 0 -3 -1 -2 -3 2 -2 -4 4 -1 -1 -3 -3 -2 5 13 0 0 0 42 0 0 0 4 0 11 0 0 23 0 0 0 0 2 0.62 0.23 61 G -1 -2 0 -1 -3 3 0 5 -2 -3 -3 -1 2 -3 -3 -1 -2 -3 -3 -3 0 0 5 0 0 25 4 56 0 0 0 0 10 0 0 0 0 0 0 0 0.66 0.23 62 I 1 3 -1 -1 -2 0 -1 -2 -2 0 -1 -1 1 -3 -2 0 3 -3 -2 1 15 22 0 3 0 3 0 0 0 2 0 0 4 0 0 0 35 0 0 16 0.28 0.23 63 N -2 -1 7 1 -3 -1 -1 -1 0 -4 -4 -1 -3 -4 -3 1 0 -4 -3 -4 0 0 85 3 0 0 0 0 0 0 0 0 0 0 0 12 0 0 0 0 0.97 0.23 64 T 1 -3 -2 -3 3 -2 -2 -3 -3 0 -1 -2 -1 -2 -2 0 4 -3 -2 3 9 0 0 0 8 0 0 0 0 0 2 0 0 0 0 0 45 0 0 36 0.41 0.23 65 G 0 -3 -1 -2 -3 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.38 0.23 66 L -2 -3 -4 -4 -2 -3 -4 -4 -4 2 4 -3 2 0 -3 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 11 82 0 0 0 0 0 0 0 0 7 0.58 0.15 67 A 3 -2 -1 -1 -1 -1 -1 2 -2 -2 -3 -1 -2 -3 -1 3 0 -3 -2 -1 43 0 0 0 0 0 0 14 0 0 0 0 0 0 0 42 0 0 0 0 0.42 0.10 K Lambda Standard Ungapped 0.1356 0.3209 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1640 0.3170 PSI Gapped 0.0502 0.2670