Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 G 0 -2 0 -1 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 0 -2 -3 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 2 N -2 0 6 1 -3 0 0 0 1 -3 -3 0 -2 -3 -2 1 0 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 3 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 4 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.53 0.11 5 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.21 0.11 6 G -1 -2 -1 -1 -3 -1 2 4 -2 -4 -4 -1 -3 -4 4 -1 -2 -3 -3 -3 0 0 0 0 0 0 25 48 0 0 0 0 0 0 27 0 0 0 0 0 0.76 0.11 7 E -1 1 -1 -1 -3 1 3 -3 -1 -1 -2 4 -1 -3 -2 -1 -1 -4 -2 2 0 0 0 0 0 0 24 0 0 0 0 49 0 0 0 0 0 0 0 27 0.32 0.11 8 T 0 -2 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.66 0.11 9 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.53 0.11 10 S 1 -1 0 -1 -1 0 0 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.61 0.11 11 A 2 -2 -1 -1 -3 -1 2 3 -2 -3 -3 -1 -2 -4 4 0 -1 -3 -3 -2 24 0 0 0 0 0 24 27 0 0 0 0 0 0 25 0 0 0 0 0 0.55 0.11 12 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.57 0.11 13 Q -1 1 0 -1 -4 7 2 -2 0 -3 -3 1 -1 -4 -2 0 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.11 14 V -1 -3 -3 -4 -1 -2 -3 -4 -3 2 1 -3 4 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 0 0 0 27 0 0 0 0 0 0 73 0.43 0.11 15 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.53 0.11 16 L -2 0 -2 -1 -3 0 3 -3 -2 0 3 2 0 -1 -2 -1 -1 -3 -2 -1 0 0 0 0 0 0 27 0 0 0 49 24 0 0 0 0 0 0 0 0 0.26 0.11 17 K -1 0 3 3 -3 0 0 -2 -1 -1 -2 2 -1 -3 -2 -1 -1 -4 -2 1 0 0 25 24 0 0 0 0 0 0 0 24 0 0 0 0 0 0 0 27 0.28 0.11 18 G -1 -2 3 3 -3 -1 -1 4 -1 -4 -4 -1 -3 -4 -2 0 -1 -4 -3 -4 0 0 25 27 0 0 0 48 0 0 0 0 0 0 0 0 0 0 0 0 0.68 0.11 19 K -1 2 -1 0 -4 1 3 -2 -1 -3 -3 5 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 25 0 0 0 0 75 0 0 0 0 0 0 0 0 0.61 0.11 20 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.53 0.11 21 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.53 0.11 22 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.53 0.11 23 N 0 -2 3 -1 -2 -1 -1 -2 -1 0 -2 -1 -1 -3 -2 2 3 -3 -2 2 0 0 24 0 0 0 0 0 0 0 0 0 0 0 0 27 25 0 0 24 0.28 0.11 24 E 2 -2 -1 3 -2 0 3 -2 -2 2 -1 -1 -1 -2 -2 -1 -1 -4 -3 0 25 0 0 27 0 0 24 0 0 24 0 0 0 0 0 0 0 0 0 0 0.27 0.11 25 V -1 -3 -3 -3 -2 -3 -3 2 -3 3 2 -3 1 -1 -3 -2 -1 -3 -2 2 0 0 0 0 0 0 0 24 0 25 27 0 0 0 0 0 0 0 0 24 0.29 0.11 26 H -2 3 0 -1 -4 3 0 -3 7 -4 -3 0 -2 -2 -2 -1 -2 -3 0 -3 0 25 0 0 0 24 0 0 51 0 0 0 0 0 0 0 0 0 0 0 0.69 0.11 27 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.53 0.11 28 R -2 5 -1 -2 -3 1 -1 -3 -1 -2 -1 3 3 -2 -2 -1 -1 -3 -2 -2 0 48 0 0 0 0 0 0 0 0 0 27 25 0 0 0 0 0 0 0 0.49 0.11 29 I -1 -1 -3 -3 -2 -2 -2 -3 -3 4 0 2 0 -1 -3 -2 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 49 0 24 0 0 0 0 0 0 0 27 0.29 0.11 30 R -2 3 -2 -2 -3 0 -1 -3 0 -2 -2 3 -1 4 -3 -2 -2 0 4 -2 0 24 0 0 0 0 0 0 0 0 0 27 0 25 0 0 0 0 24 0 0.43 0.11 31 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.53 0.11 32 K -1 0 -1 -1 -4 0 3 -2 -2 -3 -3 2 -3 -4 6 -1 -1 -4 -3 -3 0 0 0 0 0 0 27 0 0 0 0 24 0 0 49 0 0 0 0 0 0.98 0.11 33 Y -2 -1 5 0 -3 0 -1 -2 5 -3 -3 -1 -2 0 -3 -1 -1 -1 4 -3 0 0 49 0 0 0 0 0 27 0 0 0 0 0 0 0 0 0 24 0 0.61 0.11 34 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.21 0.11 35 L -3 -3 -4 -4 -3 -3 -4 -4 -2 0 2 -4 0 6 -4 -3 -2 0 2 -1 0 0 0 0 0 0 0 0 0 0 24 0 0 76 0 0 0 0 0 0 0.74 0.11 36 K -1 2 -1 -1 -4 1 1 -2 -1 -3 -3 6 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.70 0.11 37 K -1 1 -1 -2 -3 0 0 -2 -2 -1 -2 5 -1 -3 -2 -1 -1 -4 -2 1 0 0 0 0 0 0 0 0 0 0 0 75 0 0 0 0 0 0 0 25 0.41 0.11 38 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.13 0.11 39 E -2 -1 0 4 -4 1 5 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 27 0 0 73 0 0 0 0 0 0 0 0 0 0 0 0 0 0.80 0.11 40 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.94 0.11 41 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.21 0.11 42 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.53 0.11 43 E -1 -1 -1 1 -3 1 5 -2 -1 -3 -3 0 -2 -3 -2 0 2 -3 -2 -2 0 0 0 0 0 0 76 0 0 0 0 0 0 0 0 0 24 0 0 0 0.64 0.11 44 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -1 -1 -3 -1 5 -4 -2 -2 2 7 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 24 0 0 0 0 76 0 0.96 0.11 45 P 2 -2 -2 -2 -2 -2 -1 -2 -3 -3 -3 -1 -2 -4 7 0 -1 -4 -3 -2 27 0 0 0 0 0 0 0 0 0 0 0 0 0 73 0 0 0 0 0 1.45 0.11 46 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.53 0.11 47 S 0 -2 -1 -2 -1 -1 -2 -2 -2 2 -1 -1 -1 -2 -2 2 4 -3 -2 0 0 0 0 0 0 0 0 0 0 27 0 0 0 0 0 24 49 0 0 0 0.33 0.11 48 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.53 0.11 49 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.57 0.11 50 K -2 0 1 6 -4 0 1 -2 -1 -4 -4 3 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 67 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0.71 0.09 51 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.47 0.05 K Lambda Standard Ungapped 0.1373 0.3227 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1514 0.3175 PSI Gapped 0.0463 0.2670