Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -1 -2 -3 -4 -2 -1 -3 -3 -2 1 2 -2 7 0 -3 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.70 0.15 2 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.59 0.15 3 S 1 -1 0 -1 -1 -1 -1 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.66 0.15 4 K -1 2 -1 -1 -4 2 1 -2 -1 -3 -3 5 -2 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 11 0 0 0 0 0 89 0 0 0 0 0 0 0 0 0.71 0.15 5 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.68 0.15 6 S 0 -1 0 -1 -1 -1 -1 -1 -2 -2 -3 -1 -2 -3 -1 4 3 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 74 26 0 0 0 0.56 0.15 7 Q 1 -1 0 -1 -2 4 0 -2 4 -3 -3 0 -2 -3 2 2 0 -3 -1 -2 12 0 0 0 0 33 0 0 14 0 0 0 0 0 10 31 0 0 0 0 0.42 0.15 8 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.68 0.15 9 Q -1 1 0 -1 -4 7 2 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.92 0.15 10 T 0 1 -1 -2 -2 4 0 -2 1 -1 -1 0 1 -3 -2 0 2 -3 -2 1 5 7 0 0 0 36 0 0 5 0 0 0 5 0 0 4 23 0 0 14 0.28 0.15 11 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.96 0.15 12 M -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 3 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 94 0 6 0 0 0 0 0 0 0 0.65 0.15 13 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.68 0.15 14 A 3 -2 2 -1 -2 -1 -1 2 -2 -3 -3 -1 -2 -3 -2 3 1 -3 -2 -2 37 0 11 0 0 0 0 16 0 0 0 0 0 0 0 31 5 0 0 0 0.39 0.15 15 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.62 0.15 16 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.68 0.15 17 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.68 0.15 18 E -1 -1 1 3 -3 1 4 -2 -1 -3 -3 0 -3 -4 -2 2 0 -4 -3 -3 0 0 6 21 0 0 47 0 0 0 0 0 0 0 0 20 5 0 0 0 0.56 0.15 19 S 1 -1 0 -1 -1 -1 -1 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.66 0.15 20 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.27 0.15 21 L 0 -3 -4 -4 -2 -3 -3 -3 -3 1 4 -3 1 -1 -3 -2 -1 -3 -2 3 11 0 0 0 0 0 0 0 0 0 57 0 0 0 0 0 0 0 0 32 0.41 0.15 22 S 0 -1 0 -1 -1 -1 -1 -2 -2 -2 -2 -1 -1 -3 -1 3 5 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 37 63 0 0 0 0.56 0.15 23 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 6 0 0 0 0 0 0 0 0 0 94 0 0 0 0 0 0 0 0 0.74 0.15 24 E -1 -1 0 2 -4 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 11 0 0 89 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.15 25 A 3 -2 -1 0 -1 -2 -1 0 -2 0 -1 -2 -1 -2 -2 0 3 -3 -2 2 37 0 0 6 0 0 0 7 0 0 0 0 0 0 0 0 29 0 0 22 0.26 0.15 26 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 0 91 0 0 0 0 0 0 0 0 9 0.62 0.15 27 I -2 -3 -4 -4 -2 -3 -4 -4 -4 5 2 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 79 10 0 0 0 0 0 0 0 0 11 0.57 0.15 28 Q -1 3 1 -1 -4 5 1 -2 0 -3 -3 2 -1 -4 -2 -1 -1 -3 -2 -3 0 22 9 0 0 55 0 0 0 0 0 14 0 0 0 0 0 0 0 0 0.63 0.15 29 A 5 -2 -2 -2 -1 -1 -1 1 -2 -2 -2 -1 -2 -3 -1 1 0 -3 -2 -1 86 0 0 0 0 0 0 5 0 0 0 0 0 0 0 9 0 0 0 0 0.54 0.15 30 L -2 -3 -4 -4 -2 -2 -3 -4 -3 1 5 -3 3 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 91 0 9 0 0 0 0 0 0 0 0.63 0.15 31 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -2 -1 -2 -3 -3 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.15 0.07 K Lambda Standard Ungapped 0.1240 0.3107 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1605 0.3159 PSI Gapped 0.0491 0.2670