Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.53 0.09 2 E 0 -2 -1 -1 -2 0 4 -2 -2 -2 -3 -1 -2 -3 -2 0 5 -4 -3 -2 8 0 0 0 0 0 37 0 0 0 0 0 0 0 0 0 55 0 0 0 0.63 0.31 3 T 1 -2 -2 -2 -2 -2 -2 -2 -3 -3 -3 -2 -2 -4 6 1 4 -4 -3 -2 15 0 0 0 0 0 0 0 0 0 0 0 0 0 40 7 37 0 0 0 0.90 0.32 4 V -2 -2 0 5 -4 0 4 -3 -2 -3 -3 -1 -3 -4 -2 -1 -2 -4 -3 0 0 0 0 49 0 0 40 0 0 0 0 0 0 0 0 0 0 0 0 11 0.83 0.32 5 E 1 -1 -1 0 -4 1 6 -2 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 18 0 0 0 0 0 82 0 0 0 0 0 0 0 0 0 0 0 0 0 0.92 0.32 6 S 0 -3 -3 -2 -2 -2 2 -3 -3 3 -1 -2 -1 -2 -3 1 -1 -4 -2 4 7 0 0 0 0 0 18 0 0 24 0 0 0 0 0 11 0 0 0 39 0.36 0.32 7 C -1 -5 -4 -5 10 -4 -5 -4 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -4 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.16 0.38 8 L -2 -2 -3 -2 -3 0 2 -4 -3 3 3 1 3 -2 -3 -2 -2 -3 -2 0 0 0 0 0 0 5 19 0 0 25 31 9 10 0 0 0 0 0 0 0 0.37 0.38 9 A 4 -2 2 -2 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 4 1 -4 -3 -2 41 0 10 0 0 0 0 0 0 0 0 0 0 0 0 43 5 0 0 0 0.59 0.38 10 K -2 4 -1 -2 -4 2 0 -3 -1 -4 -3 5 -2 -4 -2 0 -2 -4 -3 -3 0 25 0 0 0 9 0 0 0 0 0 60 0 0 0 5 0 0 0 0 0.83 0.38 11 S -1 -3 -2 -2 -4 0 -2 -3 -3 -4 -4 -2 -3 -5 8 1 -2 -5 -4 -3 0 0 0 0 0 5 0 0 0 0 0 0 0 0 80 15 0 0 0 0 1.82 0.38 12 H 2 -3 -2 -2 -2 -2 0 -2 3 0 -2 -2 -2 -3 4 1 1 -4 -2 1 20 0 0 0 0 0 6 0 9 7 0 0 0 0 24 10 7 0 0 16 0.43 0.38 13 T -2 -3 -3 -2 -3 -2 2 -4 -3 3 3 -2 0 3 -3 -2 1 -3 -1 0 0 0 0 0 0 0 20 0 0 21 35 0 0 15 0 0 9 0 0 0 0.38 0.38 14 E 0 -1 1 4 -4 2 3 -2 -2 -4 -4 1 -3 -4 -2 2 -1 -4 -3 -3 7 0 7 31 0 11 18 0 0 0 0 9 0 0 0 16 0 0 0 0 0.55 0.38 15 N 0 -2 3 -2 -3 -2 -2 5 -2 -4 -4 0 -3 1 -3 -1 -2 -3 -3 -4 6 0 23 0 0 0 0 57 0 0 0 7 0 7 0 0 0 0 0 0 0.79 0.37 16 S 0 -2 -1 -2 -2 -2 -2 -2 -3 -1 -2 -2 -2 -3 -2 4 4 -4 -3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 40 40 0 0 20 0.57 0.37 17 F -3 -4 -4 -5 -3 -4 -4 -4 -2 0 2 -4 2 6 -4 -3 -3 -1 3 -1 0 0 0 0 0 0 0 0 0 0 29 0 6 59 0 0 0 0 7 0 0.92 0.37 18 T 0 -1 4 -1 -2 -1 -1 -1 -1 -3 -3 -1 -2 -3 -2 4 2 -3 -2 -2 1 1 25 1 0 0 1 1 0 1 1 1 0 0 1 52 15 0 0 1 0.59 0.33 19 N -2 -2 6 1 -3 -1 -1 2 -1 -4 -4 -1 -3 -3 2 -1 -1 -4 -3 -3 1 1 56 7 0 0 1 20 0 1 1 1 0 0 10 1 1 0 0 1 0.74 0.33 20 V 3 -3 -3 -3 -1 -2 -2 -2 -3 1 -1 -2 -1 -2 -2 -1 1 -3 -2 4 29 1 0 1 0 0 1 1 0 1 1 1 0 0 1 1 7 0 0 55 0.46 0.33 21 W -2 1 -3 -3 -3 -2 -3 -3 -1 -2 -2 -2 -2 4 -3 0 -2 8 5 -2 1 10 0 0 0 0 0 1 0 0 1 0 0 23 0 11 0 24 27 0 0.90 0.34 22 K -1 3 -1 -2 -3 0 0 -2 -1 -3 -3 5 -2 -3 -2 1 1 -3 -2 -2 1 20 1 1 0 1 1 1 0 1 1 52 0 0 1 8 11 0 0 1 0.58 0.33 23 D -1 -1 0 4 -3 -1 2 -2 -1 0 -2 1 -2 -2 -2 2 -1 -3 0 -2 1 1 1 35 0 1 11 1 0 8 1 11 0 1 1 20 1 0 4 1 0.39 0.31 24 D -1 -2 1 4 -3 -1 -1 3 -2 -4 -4 1 -3 -4 -2 2 -1 -4 -3 -3 1 0 5 36 0 0 0 27 0 0 1 10 0 0 0 17 0 0 0 0 0.58 0.36 25 K -1 -1 3 4 -3 1 0 -2 -1 -2 -2 1 -2 -2 -2 0 -1 -2 1 0 2 1 19 29 0 6 1 2 0 1 2 9 0 1 1 7 1 0 7 11 0.33 0.31 26 T -1 -1 -1 2 -2 -1 0 1 -2 -2 -3 0 -2 -3 -2 2 3 -3 -2 -2 1 1 1 13 0 1 6 10 0 1 1 7 0 1 1 24 32 0 0 1 0.37 0.33 27 L -1 -2 -2 -2 -2 -2 -2 4 -2 -2 0 -2 -1 -2 -2 0 -2 7 -1 -2 2 1 1 1 0 1 1 41 0 1 15 1 0 1 1 11 1 18 1 1 0.62 0.31 28 D -1 -1 4 4 -2 -1 0 1 -1 -2 -3 -1 -2 -3 -2 0 0 -3 -2 -2 2 1 30 28 1 1 2 13 1 1 2 2 1 1 1 2 8 0 1 2 0.46 0.28 29 R -2 5 -2 -2 -3 -1 -1 -1 -1 -2 -2 1 -2 1 -2 -2 -2 -1 4 -2 1 42 1 1 0 1 1 6 0 1 2 8 0 6 1 1 1 0 25 1 0.55 0.32 30 Y -3 -3 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 2 -4 -3 -2 1 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 89 11 1.35 0.35 31 A 4 -3 -3 -3 -2 -2 -2 -2 -3 3 -1 -2 -1 -2 -2 -1 -1 -4 -3 2 61 0 0 0 0 0 0 0 0 22 0 0 0 0 0 0 0 0 0 17 0.53 0.35 32 N -2 -2 6 0 -3 -1 -2 -2 -1 0 -3 -2 -2 -2 -3 -1 0 -3 1 0 0 0 67 0 0 0 0 0 0 7 0 0 0 0 0 0 6 0 8 11 0.72 0.35 33 Y -2 -3 -4 -4 -3 -3 -3 -4 -1 1 1 -3 -1 4 -4 -3 -2 0 6 2 0 0 0 0 0 0 0 0 0 6 12 0 0 16 0 0 0 0 45 21 0.74 0.35 34 E -2 -1 3 4 -4 1 4 -2 2 -4 -4 -1 -3 -4 -2 -1 -2 -4 -3 -4 0 0 19 30 0 6 39 0 6 0 0 0 0 0 0 0 0 0 0 0 0.78 0.35 35 G -1 -3 2 -2 -4 -3 -3 6 -3 -5 -5 -2 -4 -4 -3 -1 -2 -4 -4 -4 0 0 11 0 0 0 0 89 0 0 0 0 0 0 0 0 0 0 0 0 1.33 0.35 36 C -1 -5 -4 -5 10 -4 -5 -4 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -3 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.10 0.35 37 L -2 -2 -2 -2 -3 1 3 -4 -2 4 1 1 -1 -2 -3 -2 -2 -4 -3 0 0 0 0 0 0 5 30 0 0 41 13 11 0 0 0 0 0 0 0 0 0.40 0.35 38 W -3 -3 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 2 -4 -3 -3 6 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13 87 0 1.54 0.35 39 N -2 0 2 0 -4 0 5 -3 -1 1 -2 -1 1 -3 -3 -1 -2 -4 -3 -2 0 5 13 0 0 0 62 0 0 14 0 0 6 0 0 0 0 0 0 0 0.63 0.35 40 A 5 -2 -2 -3 -1 -2 -2 -1 -3 -2 0 -2 -1 -3 -2 1 -1 -3 -3 -1 78 0 0 0 0 0 0 0 0 0 11 0 0 0 0 11 0 0 0 0 0.64 0.35 41 T -1 -2 1 -2 -2 -1 -2 -2 -2 -2 -3 0 -2 -3 -2 2 6 -4 -3 -1 0 0 8 0 0 0 0 0 0 0 0 5 0 0 0 11 75 0 0 0 0.76 0.35 42 G -1 -3 -1 1 -3 -2 -2 6 -3 -5 -5 -2 -4 -4 -3 1 -2 -4 -4 -4 0 0 0 8 0 0 0 81 0 0 0 0 0 0 0 11 0 0 0 0 1.15 0.35 43 V -1 -3 0 -3 -2 -3 -3 -4 -4 3 0 -3 0 -2 0 -2 -1 -4 -2 5 0 0 7 0 0 0 0 0 0 22 0 0 0 0 6 0 0 0 0 65 0.61 0.35 44 V -2 -4 -3 -4 -2 -3 -4 2 -4 4 1 -3 0 -2 -4 -2 0 -4 -3 3 0 0 0 0 0 0 0 22 0 40 14 0 0 0 0 0 4 0 0 20 0.46 0.35 45 V -1 -3 -4 -4 -2 -3 -3 -4 -4 3 1 -3 0 -2 -3 -1 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 24 8 0 0 0 0 6 0 0 0 62 0.64 0.35 46 C -1 -4 -3 -4 8 -4 -4 3 -4 1 -2 -4 -2 -3 -4 -2 -2 -3 -3 0 0 0 0 0 55 0 0 27 0 11 0 0 0 0 0 0 0 0 0 7 1.07 0.35 47 T -1 -2 2 -2 -3 2 -1 2 -1 -2 -3 -1 -2 -2 -3 2 1 -3 2 0 0 0 12 0 0 15 0 21 0 0 0 0 0 0 0 17 13 0 11 11 0.30 0.35 48 G -1 -2 3 3 -4 -1 2 4 -2 -4 -4 -1 -3 -4 -3 0 -2 -4 -4 -4 0 0 17 17 0 0 20 41 0 0 0 0 0 0 0 6 0 0 0 0 0.68 0.35 49 D -1 -2 2 6 -4 -1 0 -2 -2 -4 -4 1 -3 -4 -2 -1 1 -5 -4 -3 6 0 11 63 0 0 0 0 0 0 0 8 0 0 0 0 11 0 0 0 0.84 0.35 50 E -1 1 -1 2 -3 -1 2 3 -2 -4 -4 1 -3 -4 -2 2 -1 -4 -3 -4 0 11 0 17 0 0 13 29 0 0 0 6 0 0 0 25 0 0 0 0 0.48 0.35 51 T -1 -2 0 1 -2 -2 -2 2 -3 1 -2 -2 -2 -3 -2 0 5 -4 -3 -1 0 0 4 11 0 0 0 16 0 11 0 0 0 0 0 0 58 0 0 0 0.52 0.35 52 Q -1 -2 -2 -2 -2 2 -2 -3 2 2 -1 -2 -1 -2 -2 1 1 -3 -2 3 1 1 0 1 0 13 1 1 6 13 1 1 0 0 1 12 9 0 0 39 0.28 0.31 53 C 0 -3 -2 -3 9 -2 -3 -2 -3 -1 -2 -2 -2 -2 -2 -1 -1 -2 -2 0 8 1 1 1 66 1 1 2 0 1 2 1 0 1 1 2 1 0 1 9 1.12 0.27 54 Y 4 -2 2 -2 -2 -2 -2 -1 -1 -3 -3 -2 -2 -1 -2 2 0 -2 4 -2 49 0 11 0 0 0 0 0 0 0 0 0 0 0 0 13 6 0 21 0 0.48 0.35 55 G 5 -3 -2 -3 -2 -2 -2 3 -3 -3 -3 -2 -2 -3 -2 1 0 -4 -3 -2 66 0 0 0 0 0 0 23 0 0 0 0 0 0 0 4 6 0 0 0 0.66 0.35 56 T -2 -2 2 4 -3 -1 -1 -2 3 -3 -3 1 -3 -4 -2 0 4 -4 -2 -3 0 0 14 27 0 0 0 0 11 0 0 8 0 0 0 4 36 0 0 0 0.54 0.35 57 W -4 -4 -5 -5 -3 -3 -4 -4 -3 -4 -3 -4 -2 0 -5 -4 -3 12 1 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 3.10 0.35 58 V -2 2 1 -2 -3 -1 -1 -3 -2 2 -2 4 -1 -3 -3 -1 1 -4 -3 0 0 11 8 0 0 0 0 0 0 19 0 43 0 0 0 0 11 0 0 8 0.39 0.35 59 P -2 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -4 -5 8 -2 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.43 0.35 60 I -1 -3 -2 -3 -2 -3 -3 -4 -4 2 -1 -3 -1 -2 -3 -1 4 -4 -2 4 0 0 0 0 0 0 0 0 0 14 0 0 0 0 0 0 37 0 0 49 0.56 0.32 61 G 2 1 -2 -2 -3 -2 -2 5 -3 -4 -4 -1 -3 -4 -3 -1 -2 -4 -3 -3 27 12 0 0 0 0 0 62 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.32 62 L 3 -2 -3 -3 -2 -2 -2 -2 -3 0 0 2 -1 -2 -2 -1 -1 -4 -3 3 41 0 0 0 0 0 0 0 0 0 10 19 0 0 0 0 0 0 0 30 0.36 0.34 63 A 4 -2 -2 -3 -2 -2 -2 -2 -1 -1 -2 -2 -2 -1 -2 0 2 -1 4 1 53 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 0 24 10 0.49 0.32 64 I 0 -2 -2 2 -3 -1 3 -3 -2 3 -1 -2 -1 -2 -3 -2 -1 -4 -3 3 11 0 0 14 0 0 27 0 0 22 0 0 0 0 0 0 0 0 0 26 0.33 0.30 65 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.30 0.22 K Lambda Standard Ungapped 0.1318 0.3160 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1585 0.3172 PSI Gapped 0.0485 0.2670