Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -2 -2 -3 -4 -2 -1 -3 -3 -2 1 2 -2 8 0 -3 -2 -1 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.75 0.17 2 K -2 4 -1 -2 -4 5 1 -3 -1 -4 -3 3 -2 -4 -2 -1 -2 -3 -2 -3 0 36 0 0 0 38 0 0 0 0 0 27 0 0 0 0 0 0 0 0 0.70 0.20 3 H -3 -2 1 6 -4 -1 1 -2 5 -4 -4 -1 -3 -4 -2 -1 -2 -5 -2 -4 0 0 0 73 0 0 0 0 27 0 0 0 0 0 0 0 0 0 0 0 0.99 0.20 4 F -2 -4 -4 -4 -2 -4 -4 -4 -3 3 1 -4 0 5 -4 -3 -2 -1 1 3 0 0 0 0 0 0 0 0 0 24 11 0 0 38 0 0 0 0 0 27 0.52 0.20 5 L -2 1 -1 -2 -3 0 0 -3 -2 -2 2 5 -1 -2 -2 -1 -1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 27 73 0 0 0 0 0 0 0 0 0.50 0.20 6 T -1 -1 -1 -2 -2 -1 -1 -2 -2 -2 -2 1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 12 0 0 0 0 88 0 0 0 0.67 0.20 7 Y -3 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 3 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.30 0.20 8 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 4 -3 1 2 3 -3 -2 -2 -1 0 0 0 0 0 0 0 0 0 0 0 74 0 0 12 14 0 0 0 0 0 0.57 0.20 9 S 0 -2 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 3 5 1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 86 0 0 0 0 0.69 0.20 10 T 1 -2 -2 -3 -1 -2 -2 -2 -3 0 -1 -2 -1 -2 -2 0 4 -3 -2 3 14 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 50 0 0 36 0.41 0.20 11 A 5 -2 -2 -3 -1 -1 -2 0 -2 -2 -2 -1 -2 -3 -1 1 -1 -3 -3 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.69 0.20 12 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -5 8 -1 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.28 0.20 13 V -1 -3 -4 -4 -1 -3 -3 -4 -4 2 0 -3 0 -2 -3 -2 -1 -4 -2 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.67 0.20 14 L -2 -3 -4 -4 -2 -3 -3 -4 -4 1 4 -3 1 -1 2 -3 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 0 75 0 0 0 12 0 0 0 0 12 0.56 0.20 15 A 4 -2 -1 -2 -1 -1 -1 -1 -2 -2 -3 -1 -2 -3 -1 3 0 -3 -3 -1 64 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36 0 0 0 0 0.55 0.20 16 A 2 -2 -2 -3 -2 -2 -2 -2 -3 1 1 -2 3 -2 -2 0 3 -3 -2 0 27 0 0 0 0 0 0 0 0 11 12 0 14 0 0 0 36 0 0 0 0.29 0.20 17 I -2 -3 -3 -3 -2 -2 -3 -3 -3 2 4 -3 1 -1 -3 0 -1 -3 -2 0 0 0 0 0 0 0 0 0 0 15 59 0 0 0 0 14 0 0 0 0 0.30 0.20 18 W -2 2 -3 -3 4 -2 -2 -3 -2 -3 -2 -2 -2 -1 -3 0 -2 10 0 -3 0 14 0 0 11 0 0 0 0 0 0 0 0 0 0 12 0 51 0 0 1.14 0.20 19 M -2 -3 -3 -4 4 -3 -3 -4 -2 0 1 -3 3 6 -4 -3 -2 -1 1 -1 0 0 0 0 11 0 0 0 0 0 12 0 15 51 0 0 0 0 0 0 0.49 0.20 20 T -1 -2 -1 -1 -3 -1 1 5 -2 -3 -3 -1 -3 -3 -2 1 1 -3 -3 -3 0 0 0 0 0 0 12 50 0 0 0 0 0 0 0 11 15 0 0 0 0.47 0.20 21 I 2 -2 -2 -2 -2 -2 -2 -2 -2 1 -1 -2 -1 3 -2 2 -1 -2 0 -1 24 0 0 0 0 0 0 0 0 15 0 0 0 22 0 27 0 0 0 0 0.17 0.20 22 T -1 -2 -2 -3 -2 -2 -3 -3 -3 0 4 -2 1 -1 -3 1 2 -3 -2 0 0 0 0 0 0 0 0 0 0 0 61 0 0 0 0 12 15 0 0 0 0.27 0.20 23 A 5 -2 -2 -2 -1 -1 -2 -1 -2 -2 -2 -1 -2 -3 -2 1 1 -3 -3 -1 88 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12 0 0 0 0.60 0.20 24 G -1 3 -1 -2 -4 -2 -2 6 -2 -5 -4 -1 -3 -4 -3 -1 -2 -3 -4 -4 0 24 0 0 0 0 0 76 0 0 0 0 0 0 0 0 0 0 0 0 0.95 0.20 25 I -2 -3 -3 -4 -2 -3 -3 -4 -3 2 4 -3 1 2 -3 0 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 15 59 0 0 12 0 14 0 0 0 0 0.40 0.20 26 L -1 -2 -2 -3 -2 -2 -2 -3 -3 0 3 -2 0 -1 -3 3 0 -3 -2 1 0 0 0 0 0 0 0 0 0 0 51 0 0 0 0 39 0 0 0 11 0.32 0.20 27 I -2 -4 -3 -4 -2 -3 -4 -4 -4 6 1 -3 0 -1 -3 -2 1 -3 -2 2 0 0 0 0 0 0 0 0 0 88 0 0 0 0 0 0 12 0 0 0 0.60 0.20 28 E -2 -1 -1 1 -5 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 1.03 0.20 29 F -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 3 3 -4 -3 -2 -2 -1 2 0 0 0 0 0 0 0 0 0 74 0 0 12 15 0 0 0 0 0 0 0.57 0.20 30 N -2 -1 7 1 -4 -1 -1 -1 0 -4 -4 -1 -3 -4 -3 0 -1 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.09 0.20 31 R -2 7 -1 -2 -4 0 -1 -3 3 -4 -3 2 -2 -3 -3 -1 -2 -4 -2 -3 0 88 0 0 0 0 0 0 12 0 0 0 0 0 0 0 0 0 0 0 0.97 0.20 32 F -2 -3 -3 -4 -3 -3 -3 -4 -2 0 2 -3 0 6 -4 0 -2 0 2 -1 0 0 0 0 0 0 0 0 0 0 24 0 0 64 0 12 0 0 0 0 0.65 0.20 33 Y -2 -3 -3 -4 -3 -3 -3 -3 -1 -1 -1 -3 -1 7 -4 0 -2 0 4 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 73 0 12 0 0 15 0 0.84 0.20 34 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -5 8 -1 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.28 0.20 35 D -3 -2 1 7 -4 -1 1 -2 -2 -4 -5 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.27 0.20 36 L 5 -2 -3 -3 -1 -2 -2 -1 -3 -1 1 -2 -1 -2 -2 0 -1 -3 -2 1 73 0 0 0 0 0 0 0 0 0 15 0 0 0 0 0 0 0 0 12 0.43 0.20 37 L -2 -3 -4 -4 -2 -3 -3 -4 -4 1 5 -3 1 0 3 -3 -2 -3 -2 0 0 0 0 0 0 0 0 0 0 0 86 0 0 0 14 0 0 0 0 0 0.64 0.20 38 F -1 -2 -2 -2 -2 -2 -2 -3 -3 0 -1 -2 -1 2 2 1 3 -3 -1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 15 12 12 34 0 0 27 0.32 0.20 39 H -2 -3 -2 -3 -3 -2 -3 -3 4 -1 2 -3 0 6 -4 0 -2 -1 1 -1 0 0 0 0 0 0 0 0 15 0 23 0 0 48 0 14 0 0 0 0 0.48 0.20 40 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -4 8 -1 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.21 0.14 41 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 3 -3 1 5 -4 -3 -2 0 1 0 0 0 0 0 0 0 0 0 0 0 52 0 0 48 0 0 0 0 0 0 0.58 0.11 K Lambda Standard Ungapped 0.1457 0.3346 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1689 0.3211 PSI Gapped 0.0517 0.2670