Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 E -2 -1 0 5 -4 1 5 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 40 0 0 60 0 0 0 0 0 0 0 0 0 0 0 0 0 0.83 0.13 2 K -1 1 0 -1 -4 6 1 -2 0 -3 -3 3 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 78 0 0 0 0 0 22 0 0 0 0 0 0 0 0 0.75 0.13 3 S 1 -1 0 -1 -1 -1 -1 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.64 0.13 4 M -1 -2 -3 -4 -2 -1 -3 -3 -2 1 2 -2 8 0 -3 -2 -1 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.67 0.13 5 M 4 -2 -2 -3 -1 -1 -2 -1 -2 -1 -1 -1 3 -2 -2 0 -1 -3 -2 0 78 0 0 0 0 0 0 0 0 0 0 0 22 0 0 0 0 0 0 0 0.44 0.13 6 N -2 -1 6 1 -3 -1 -1 -1 0 -3 -4 -1 -2 -4 -2 0 2 -4 -3 -3 0 0 83 0 0 0 0 0 0 0 0 0 0 0 0 0 17 0 0 0 0.78 0.13 7 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 8 Q -2 0 0 -1 -4 6 1 -2 6 -4 -3 0 -1 -3 -2 -1 -1 -3 -1 -3 0 0 0 0 0 71 0 0 29 0 0 0 0 0 0 0 0 0 0 0 0.77 0.14 9 T 2 -1 2 -1 -1 -1 -1 -1 -1 -2 -3 -1 -2 -3 -2 4 2 -3 -2 -2 18 0 13 0 0 0 0 0 0 0 0 0 0 0 0 50 19 0 0 0 0.42 0.14 10 K -1 2 -1 -1 -4 3 1 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 0 0 0 0 13 0 0 0 0 0 87 0 0 0 0 0 0 0 0 0.69 0.14 11 I -2 -3 -3 -3 -2 -3 -3 -4 -3 5 1 -3 0 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0 0 0.33 0.14 12 D -2 -2 0 6 -3 -1 0 -2 -2 -1 -2 -1 -2 -3 -2 -1 -1 -4 -3 0 0 0 0 63 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16 0.45 0.14 13 A 4 -2 -1 2 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 -1 0 -1 -3 -2 -1 60 0 0 18 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.14 14 K -2 5 -1 -2 -3 3 0 -2 -1 -3 -2 3 -2 -3 -2 -1 -1 -3 -2 -3 0 41 0 0 0 18 0 0 0 0 0 19 0 0 0 0 0 0 0 0 0.38 0.14 15 K -1 1 -1 -1 -3 1 0 -2 -1 -3 -3 5 -2 -3 -2 -1 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0.42 0.14 16 E -1 0 -1 0 -3 1 5 -2 -1 -3 -3 2 -2 -3 -2 -1 -1 -3 -2 -3 0 0 0 0 0 0 60 0 0 0 0 18 0 0 0 0 0 0 0 0 0.43 0.14 17 Q -1 0 -1 -1 -3 5 1 -2 0 -3 -2 0 -1 -1 -2 -1 -1 -1 3 -2 0 0 0 0 0 60 0 0 0 0 0 0 0 0 0 0 0 0 18 0 0.38 0.14 18 L -2 -2 -3 -3 -2 -2 -3 -3 -3 1 4 -3 1 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0 0.35 0.14 19 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -2 -1 0 -1 -3 -2 -1 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.33 0.14 20 D -2 -2 -2 2 -2 -2 -2 -3 -3 0 4 -2 1 -1 -3 -2 -2 -2 -2 0 0 0 0 19 0 0 0 0 0 0 59 0 0 0 0 0 0 0 0 0 0.19 0.14 21 A 4 -2 -2 -2 -1 -2 -2 -1 -2 -1 1 -2 -1 -2 -2 0 -1 -3 -2 -1 60 0 0 0 0 0 0 0 0 0 18 0 0 0 0 0 0 0 0 0 0.18 0.14 22 R -1 3 -1 -1 -3 1 0 -2 -1 -3 -3 5 -2 -3 -2 -1 -1 -3 -2 -3 0 19 0 0 0 0 0 0 0 0 0 59 0 0 0 0 0 0 0 0 0.38 0.14 23 R 0 2 -1 -1 -2 -1 -1 -2 -2 -2 -2 0 -2 -3 -2 2 3 -3 -2 -1 0 19 0 0 0 0 0 0 0 0 0 0 0 0 0 25 34 0 0 0 0.20 0.14 24 D -1 -2 0 3 -3 0 3 3 -1 -3 -3 -1 -3 -3 -2 -1 -2 -3 -3 -3 0 0 0 19 0 0 34 25 0 0 0 0 0 0 0 0 0 0 0 0 0.32 0.14 25 L -2 -2 -3 -3 -2 -2 -3 -3 -3 1 4 -3 1 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0 0.35 0.14 26 K -1 1 -1 -1 -3 1 0 -2 -1 -3 -3 5 -2 -3 -2 -1 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0.42 0.14 27 S -1 0 -1 -1 -2 4 0 -2 -1 -2 0 0 -1 -2 -2 1 -1 -2 -2 -2 0 0 0 0 0 44 0 0 0 0 16 0 0 0 0 19 0 0 0 0 0.18 0.14 28 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -2 -1 0 -1 -3 -2 -1 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.33 0.14 29 K -1 1 -1 -1 -3 1 0 -2 -1 -3 -3 5 -2 -3 -2 -1 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0.42 0.14 30 A 3 1 -1 -2 -2 0 0 -1 -2 -3 -3 4 -2 -3 -1 0 -1 -4 -2 -2 41 0 0 0 0 0 0 0 0 0 0 59 0 0 0 0 0 0 0 0 0.46 0.14 31 D -1 0 0 3 -4 1 4 -2 -1 -4 -4 4 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 19 0 0 41 0 0 0 0 40 0 0 0 0 0 0 0 0 0.60 0.14 32 A 5 -2 -2 -3 -1 -1 -2 -1 -2 -1 -1 -1 3 -2 -2 1 -1 -3 -2 0 84 0 0 0 0 0 0 0 0 0 0 0 16 0 0 0 0 0 0 0 0.47 0.14 33 K -1 2 -1 -1 -4 1 0 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.84 0.19 34 V 3 -2 -2 -2 -2 -2 -2 3 -3 -2 -2 -2 -2 -3 -2 0 2 -3 -3 0 46 0 0 0 0 0 0 27 0 0 0 0 0 0 0 0 19 0 0 9 0.43 0.21 35 M -1 0 1 -1 -3 3 0 -2 -1 -2 -1 3 2 -3 -2 3 0 -3 -2 -2 0 0 8 0 0 17 0 0 0 0 8 24 9 0 0 35 0 0 0 0 0.31 0.21 36 K 0 -1 -1 -2 -3 -1 -1 3 5 -4 -4 0 -3 -3 2 2 -1 -4 -1 -3 7 0 0 0 0 0 0 31 23 0 0 9 0 0 10 19 0 0 0 0 0.48 0.21 37 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 1 -1 -5 -4 -4 0 0 0 86 0 0 0 0 0 0 0 0 0 0 0 14 0 0 0 0 1.06 0.21 38 A 1 -2 -1 2 -2 -1 1 2 -2 -1 -3 -1 -2 -3 2 2 -1 -4 -3 -2 16 0 0 16 0 0 8 17 0 8 0 0 0 0 12 24 0 0 0 0 0.32 0.21 39 K -1 2 -1 -1 -4 1 0 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.86 0.21 40 T -1 -2 -2 -2 -2 -2 -2 -2 -3 0 3 -2 0 -1 -2 3 1 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 51 0 0 0 0 40 9 0 0 0 0.35 0.21 41 K -2 4 -1 -1 -4 5 1 -3 -1 -4 -3 3 -2 -4 -2 -1 -1 -3 -2 -3 0 26 0 0 0 51 0 0 0 0 0 23 0 0 0 0 0 0 0 0 0.73 0.21 42 K 1 0 -1 -1 -2 0 -1 -2 -2 -3 -3 3 -2 -3 -2 3 3 -4 -3 -2 15 0 0 0 0 0 0 0 0 0 0 32 0 0 0 27 26 0 0 0 0.40 0.21 43 V 2 -2 -3 -3 -2 1 -2 -2 -2 1 2 -2 3 -1 -3 -1 -1 -3 -2 2 26 0 0 0 0 13 0 0 0 0 26 0 10 0 0 0 0 0 0 25 0.23 0.17 44 V -1 -3 -4 -4 -2 -3 -3 -4 -4 2 2 -3 0 -1 -3 -3 -1 4 -1 5 0 0 0 0 0 0 0 0 0 0 19 0 0 0 0 0 0 8 0 73 0.52 0.17 45 E -1 -1 -1 2 -4 1 5 -3 -1 -1 -2 1 -2 -3 -2 -1 -1 -4 -3 1 0 0 0 10 0 0 63 0 0 0 0 8 0 0 0 0 0 0 0 19 0.54 0.17 46 S -1 4 -1 2 -3 0 0 -3 -2 -2 -2 2 -2 -3 -2 0 1 -4 -2 0 0 36 0 13 0 0 0 0 0 0 0 19 0 0 0 6 12 0 0 14 0.34 0.17 47 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.80 0.17 48 K 0 4 -1 -2 -4 1 0 -2 -1 -3 -3 5 -2 -4 -2 -1 -1 -4 -2 -3 10 33 0 0 0 0 0 0 0 0 0 57 0 0 0 0 0 0 0 0 0.63 0.17 49 K 3 3 -1 -2 -2 0 -1 -1 -2 -2 -2 3 1 -3 -2 0 -1 -3 -2 -2 42 19 0 0 0 0 0 0 0 0 0 33 6 0 0 0 0 0 0 0 0.38 0.17 50 A 4 -2 -2 -3 -1 -2 -2 -1 -2 -1 1 -1 0 -2 -2 0 -1 -3 -2 0 80 0 0 0 0 0 0 0 0 0 20 0 0 0 0 0 0 0 0 0 0.42 0.13 51 V -1 -3 -4 -4 -1 -3 -3 -4 -4 2 2 -3 1 -1 -3 -2 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 0 20 0 0 0 0 0 0 0 0 80 0.48 0.13 52 Q -1 3 -1 0 -4 4 3 -2 -1 -4 -3 4 -2 -4 -2 -1 -1 -3 -2 -3 0 13 0 0 0 34 20 0 0 0 0 33 0 0 0 0 0 0 0 0 0.58 0.13 53 R -2 6 -1 -2 -4 1 0 -3 -1 -4 -3 3 -2 -4 -2 -1 -2 -3 -2 -3 0 80 0 0 0 0 0 0 0 0 0 20 0 0 0 0 0 0 0 0 0.85 0.13 54 L -1 -4 -4 -4 8 -3 -4 -4 -4 2 2 -3 0 -1 -4 -2 -1 -3 -2 0 0 0 0 0 52 0 0 0 0 23 25 0 0 0 0 0 0 0 0 0 0.75 0.13 55 E -1 0 0 0 -3 3 4 -2 0 -3 -3 2 -2 -3 -1 1 -1 -3 -2 -2 1 1 1 1 0 20 44 1 0 1 1 14 0 0 1 12 1 0 0 1 0.46 0.11 56 E -1 -1 0 2 -4 2 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 9 0 0 91 0 0 0 0 0 0 0 0 0 0 0 0 0 0.88 0.13 57 Q -1 1 0 -1 -4 6 1 -2 2 -3 -3 2 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 80 0 0 7 0 0 13 0 0 0 0 0 0 0 0 0.74 0.13 58 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 59 M 1 -2 -3 -4 -2 -2 -3 -3 -3 1 4 -2 5 0 -3 -2 -1 -2 -2 0 20 0 0 0 0 0 0 0 0 0 52 0 29 0 0 0 0 0 0 0 0.38 0.13 60 K -2 1 -1 -2 -4 1 0 -2 -1 -3 -3 5 -2 -2 -2 -1 -1 7 -1 -3 0 0 0 0 0 0 0 0 0 0 0 80 0 0 0 0 0 20 0 0 0.74 0.13 61 L -2 -2 -4 -4 -2 -2 -3 -4 -3 1 4 -3 5 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 67 0 33 0 0 0 0 0 0 0 0.55 0.13 62 E -1 -1 0 3 -3 2 5 -2 -1 -4 -3 0 -2 -4 -2 2 -1 -4 -3 -3 0 0 0 13 0 12 56 0 0 0 0 0 0 0 0 20 0 0 0 0 0.60 0.13 63 V -1 -3 -4 -4 -1 -3 -3 -4 -4 3 1 -3 1 -1 -3 -2 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 13 9 0 0 0 0 0 0 0 0 79 0.50 0.13 64 Q -1 1 0 -1 -4 7 2 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.89 0.13 65 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.60 0.13 66 T -1 -2 -1 -2 -1 -1 -2 -2 -2 1 -1 -1 -1 -2 -2 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 13 0 0 0 0 0 0 87 0 0 0 0.56 0.13 67 D -2 -2 3 7 -4 -1 1 -2 -1 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 13 87 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.03 0.13 68 R -2 6 -1 -2 -4 1 0 -3 -1 -4 -3 3 -2 -4 -2 -1 -2 -3 -2 -3 0 80 0 0 0 0 0 0 0 0 0 20 0 0 0 0 0 0 0 0 0.85 0.13 69 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 70 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 71 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.97 0.13 72 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.73 0.13 K Lambda Standard Ungapped 0.1163 0.3026 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1499 0.3170 PSI Gapped 0.0459 0.2670