Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 S 0 -1 0 -1 -1 -1 -1 -1 -2 -2 -2 -1 -1 -3 -1 3 4 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 48 52 0 0 0 0.49 0.09 2 C -1 -4 -3 -4 10 -3 -4 -3 -3 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.50 0.10 3 T -1 3 -1 -2 -2 -1 -1 -3 -2 0 -1 0 -1 -2 -2 0 3 -3 -2 2 0 31 0 0 0 0 0 0 0 0 0 0 0 0 0 0 41 0 0 28 0.30 0.10 4 N -2 0 6 1 -3 0 0 -1 0 -4 -4 1 -2 -3 -2 0 0 -4 -2 -3 0 0 85 0 0 0 0 0 0 0 0 15 0 0 0 0 0 0 0 0 0.77 0.10 5 T 0 -2 0 -2 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.72 0.15 6 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.01 0.15 7 S 1 -1 0 -1 -1 -1 -1 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.66 0.15 8 Q -2 0 -1 -1 -4 7 1 -3 0 -4 -3 1 -1 -4 -2 -1 -1 3 -2 -3 0 0 0 0 0 95 0 0 0 0 0 0 0 0 0 0 0 5 0 0 1.01 0.23 9 L -2 -3 -4 -4 -2 -3 -4 -4 -2 5 1 -3 0 0 -4 -3 -2 -2 3 2 0 0 0 0 0 0 0 0 0 62 11 0 0 0 0 0 0 0 17 11 0.56 0.23 10 S 0 -1 3 -1 -2 -1 -1 -1 -1 -3 -3 -1 -2 -3 -2 5 2 -4 -2 -2 0 0 17 0 0 0 0 0 0 0 0 0 0 0 0 72 11 0 0 0 0.62 0.23 11 A 3 1 -1 -1 -2 1 2 1 -1 -2 -2 0 -2 -3 1 1 -1 -3 -2 -2 38 10 1 1 0 7 14 10 0 1 1 1 0 1 6 7 1 0 0 1 0.27 0.20 12 N -2 -1 7 0 -3 -1 -1 -1 0 -3 -3 -1 -2 -2 -2 0 -1 -3 1 -3 1 0 86 0 0 0 1 1 0 0 1 0 0 0 0 1 1 0 6 1 0.90 0.22 13 S 2 -2 0 -1 -1 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 5 1 -4 -2 -2 18 0 0 0 0 0 0 0 0 0 0 0 0 0 0 82 0 0 0 0 0.67 0.24 14 K -2 4 -1 -2 -4 4 0 -3 3 -3 -1 2 -1 -2 -3 -1 -2 3 2 -3 0 27 0 0 0 26 0 0 9 0 6 19 0 0 0 0 0 5 9 0 0.48 0.24 15 C -1 -4 -4 -4 9 -4 -4 -3 -4 -1 -1 -4 -2 -3 -4 -2 -1 -3 -3 1 0 0 0 0 82 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 1.45 0.24 16 E 0 -3 0 -1 -2 -2 -1 -3 -3 1 0 -2 2 -2 -3 -2 -1 -4 -2 4 6 0 6 5 0 0 5 0 0 0 0 0 9 0 0 0 0 0 0 70 0.39 0.24 17 K -2 4 2 0 -3 0 -1 -3 -1 1 -2 3 -2 -3 -2 0 0 -4 -2 -1 0 33 11 5 0 0 0 0 0 18 0 23 0 0 0 6 4 0 0 0 0.36 0.24 18 S 1 -1 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 5 1 -4 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.78 0.24 19 T -1 -2 -1 -2 -2 -1 -2 -2 -3 -1 -2 -1 -1 -3 -2 1 6 -3 -2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 91 0 0 9 0.76 0.24 20 L 2 -3 -3 -3 -2 -2 -2 -3 -3 0 3 -2 0 -1 2 -1 2 -3 -2 0 21 0 0 0 0 0 0 0 0 0 48 0 0 0 9 0 18 0 0 4 0.33 0.24 21 T -1 -2 1 -2 -2 -1 -2 -2 -2 -2 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 93 0 0 0 0.79 0.24 22 N -2 -1 7 1 -3 -1 -1 -1 0 -4 -4 -1 -3 -4 -3 0 -1 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.17 0.24 23 C -1 -4 -3 -4 10 -3 -4 -3 -4 -2 -2 -3 -2 -3 -3 0 -1 -3 -3 -2 0 0 0 0 91 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 1.63 0.24 24 Y -2 -2 4 -2 -3 -2 -2 -3 1 0 -2 -2 -2 1 -3 -2 -2 0 7 -2 0 0 30 0 0 0 0 0 0 9 0 0 0 0 0 0 0 0 61 0 0.77 0.24 25 V -2 -4 -4 -4 -2 -3 -4 -4 -4 5 2 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 63 13 0 0 0 0 0 0 0 0 24 0.64 0.24 26 D -2 1 2 6 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 9 9 82 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.06 0.24 27 K -2 -1 6 1 -3 -1 -1 -1 0 -4 -4 1 -3 -2 -3 0 -1 -3 1 -3 0 0 66 6 0 0 0 0 0 0 0 13 0 0 0 5 0 0 9 0 0.72 0.24 28 S 1 -1 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 5 1 -4 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.78 0.24 29 E -2 0 -1 1 -4 6 3 -3 0 -4 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 5 0 76 19 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.24 30 V -1 -3 -4 -4 -2 -3 -3 -4 -4 2 2 -3 1 -1 -3 -3 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 4 26 0 0 0 0 0 0 0 0 70 0.59 0.24 31 F -2 -2 -1 2 -3 -2 0 -1 2 -2 -2 -2 -2 4 -3 -1 -2 0 5 -2 0 0 0 20 0 0 6 6 5 0 0 0 0 22 0 5 0 0 36 0 0.53 0.24 32 G -1 1 0 2 -2 -1 -1 2 -2 -3 -2 -1 -2 -3 -2 2 3 -4 -3 -2 0 9 0 15 0 0 0 19 0 0 4 0 0 0 0 24 30 0 0 0 0.35 0.24 33 T -1 -2 -1 -2 -2 -1 -2 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.86 0.24 34 T -1 -2 -1 -2 -2 -1 -2 -2 -2 -2 -2 -1 -1 -3 1 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 0 94 0 0 0 0.81 0.24 35 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -3 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.86 0.24 36 T -1 -2 -1 -2 -2 -1 -2 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.86 0.24 37 G -1 2 4 -1 -3 -1 -2 4 -1 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -3 0 13 35 0 0 0 0 42 0 0 0 0 0 0 0 6 5 0 0 0 0.64 0.24 38 S 1 -1 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 5 1 -4 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.78 0.24 39 R -2 4 -1 -2 -4 5 0 -3 3 -4 -3 1 -2 -4 -2 -1 -2 -3 -2 -3 0 39 0 0 0 52 0 0 9 0 0 0 0 0 0 0 0 0 0 0 0.79 0.24 40 F -3 -3 -3 -4 -3 -2 -3 -4 1 -2 -1 -3 -2 4 -4 -3 -2 1 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 14 0 0 0 0 86 0 1.25 0.24 41 D -2 -2 4 5 -3 -1 1 -2 -1 -1 -3 -1 -3 -4 -2 0 1 -4 -3 -2 0 0 36 43 0 0 6 0 0 7 0 0 0 0 0 0 9 0 0 0 0.67 0.24 42 G -1 -3 3 -1 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 17 0 0 0 0 83 0 0 0 0 0 0 0 0 0 0 0 0 1.13 0.24 43 V 2 -3 -3 -4 -1 -3 -3 -3 -4 3 0 -3 0 -2 -3 -2 -1 -4 -2 4 22 0 0 0 0 0 0 0 0 21 0 0 0 0 0 0 0 0 0 57 0.48 0.24 44 T -2 0 3 -2 -3 -1 -2 -3 6 -3 -3 -1 -2 0 -3 -1 0 0 6 -3 0 5 17 0 0 0 0 0 27 0 0 0 0 0 0 0 10 0 41 0 0.68 0.24 45 I -2 -4 -4 -4 -2 -3 -4 -5 -4 6 1 -3 0 -1 -4 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 87 0 0 0 0 0 0 0 0 0 13 0.75 0.24 46 T -1 0 -1 -2 -2 -1 -2 -3 -2 -1 -1 -1 4 -2 -2 0 5 -3 -2 -1 0 5 0 0 0 0 0 0 0 0 0 0 20 0 0 0 75 0 0 0 0.60 0.22 47 T 0 -2 0 -1 -2 -1 -1 -1 -2 0 -2 -1 -2 -3 -2 4 3 -3 -2 -1 0 0 0 0 0 0 0 0 0 8 0 0 0 0 0 66 27 0 0 0 0.54 0.22 48 S 0 -1 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 5 3 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 83 17 0 0 0 0.67 0.22 49 T -1 -2 -1 -2 -2 -1 -2 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.84 0.22 50 S -1 -2 -1 -2 -2 -1 -1 -2 -2 -2 -2 -1 -1 -3 -2 2 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 90 0 0 0 0.77 0.22 51 T -1 -2 -1 -2 -2 -1 -2 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.84 0.22 52 G -1 -2 4 1 -3 -1 -1 4 -1 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -3 0 0 34 8 0 0 0 48 0 0 0 0 0 0 0 5 6 0 0 0 0.71 0.22 53 S 0 -2 -1 -2 5 -1 -2 -2 -2 -2 -2 -2 -2 -3 -2 3 4 -3 -2 -1 0 0 0 0 20 0 0 0 0 0 0 0 0 0 0 32 48 0 0 0 0.61 0.22 54 R -1 5 2 -1 -3 0 -1 -2 2 -4 -3 1 -2 -3 -3 1 -1 -4 -2 -3 0 61 12 0 0 0 0 0 8 0 0 0 0 0 0 19 0 0 0 0 0.67 0.22 55 I -1 -4 -4 -4 -2 -3 -4 -4 -4 6 1 -3 0 -1 -3 -3 -1 -3 -2 2 6 0 0 0 0 0 0 0 0 87 0 0 0 0 0 0 0 0 0 6 0.67 0.22 56 S 0 2 0 -1 -2 -1 -1 -2 -2 -2 -1 0 -2 -3 -2 4 3 -3 -2 -2 0 15 0 0 0 0 0 0 0 0 6 0 0 0 0 58 21 0 0 0 0.44 0.21 57 G -1 -3 2 -1 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -3 -1 0 -3 -3 -3 0 0 16 0 0 0 0 75 0 0 0 0 0 0 0 0 9 0 0 0 0.96 0.22 58 P -1 -2 -2 -2 -3 -2 -2 -2 -3 -1 -3 -2 -2 -4 7 2 -1 -4 -3 -2 0 0 0 0 0 0 0 0 0 8 0 0 0 0 68 25 0 0 0 0 1.36 0.22 59 G 2 -1 -1 -2 -3 4 0 4 -1 -3 -3 -1 -2 -4 -2 0 -1 -3 -3 -3 21 0 0 0 0 33 0 46 0 0 0 0 0 0 0 0 0 0 0 0 0.59 0.17 60 C -1 -4 -3 -4 9 -3 -4 -3 -4 0 -1 -3 -1 -2 -3 -2 -1 -3 -3 2 0 0 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0 33 1.06 0.17 61 K 0 -1 -1 -2 -2 -1 -1 -2 -1 -2 0 2 -1 0 -2 3 1 -1 4 -2 0 0 0 0 0 0 0 0 0 0 11 18 0 0 0 39 10 0 22 0 0.28 0.17 62 I -1 -3 -3 -3 -2 -3 -3 -4 -3 5 1 -3 0 -1 -3 -2 2 -3 -2 2 0 0 0 0 0 0 0 0 0 67 0 0 0 0 0 0 22 0 0 11 0.47 0.17 63 S 0 -1 0 -1 -1 -1 -1 -1 -2 -3 -3 -1 -2 -3 -1 5 3 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 78 22 0 0 0 0.60 0.17 64 T 0 1 0 -1 -2 -1 -1 -2 2 -2 -2 -1 -2 -3 -2 2 4 -3 -2 -1 0 10 0 0 0 0 0 0 9 0 0 0 0 0 0 21 59 0 0 0 0.47 0.17 65 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.70 0.17 66 I -1 -2 -1 -2 -1 -1 -2 -2 -2 2 -1 -1 0 -2 -2 1 5 -3 -2 1 0 0 0 0 0 0 0 0 0 26 0 0 0 0 0 0 74 0 0 0 0.43 0.10 67 I -2 -3 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.57 0.10 68 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.64 0.10 69 G -1 5 -1 -2 -3 0 -1 4 -1 -4 -3 1 -2 -3 -3 -1 -2 -3 -3 -3 0 59 0 0 0 0 0 41 0 0 0 0 0 0 0 0 0 0 0 0 0.66 0.10 70 G 0 -3 -1 -2 -3 -2 -3 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.20 0.10 71 V 0 -3 -2 -3 -1 -2 -2 -3 -3 2 0 -2 0 -1 -2 0 0 -3 -2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15 0 0 0 85 0.38 0.10 72 P 3 -2 -2 -2 -2 -2 -2 -1 -2 -1 -2 -1 -1 -3 5 0 -1 -3 -2 1 43 0 0 0 0 0 0 0 0 0 0 0 0 0 41 0 0 0 0 17 0.72 0.10 73 A 4 -2 -1 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -3 -1 1 3 -3 -2 0 70 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 0 0 0 0.43 0.10 74 P -1 -3 -2 -2 -3 -2 -1 -3 -3 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.17 0.10 75 S 3 -1 0 -1 -1 -1 -1 0 -1 -2 -3 -1 -2 -3 -1 4 1 -3 -2 -2 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 72 0 0 0 0 0.48 0.10 76 A 4 -2 2 -1 -1 -1 -1 0 -2 -2 -2 -1 -2 -3 3 1 0 -3 -2 -1 69 0 17 0 0 0 0 0 0 0 0 0 0 0 15 0 0 0 0 0 0.45 0.10 77 A 4 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 3 1 0 -3 -2 -1 85 0 0 0 0 0 0 0 0 0 0 0 0 0 15 0 0 0 0 0 0.55 0.10 78 C -1 -4 -3 -4 10 -3 -4 -3 -3 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.50 0.10 79 K -1 5 -1 -1 -4 1 0 -2 -1 -3 -3 4 -2 -3 -2 -1 -1 -3 -2 -3 0 45 0 0 0 0 0 0 0 0 0 55 0 0 0 0 0 0 0 0 0.67 0.10 80 I -2 -3 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.57 0.10 81 S 1 -1 0 -1 -1 0 0 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.59 0.10 82 G 0 -3 -1 -2 -3 -2 -3 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.20 0.10 83 C -1 -4 -3 -4 10 -3 -4 -3 -3 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.50 0.10 84 T 0 -2 -1 -2 -1 -1 -1 -2 -2 1 -1 -1 -1 -2 -2 2 5 -3 -2 0 0 0 0 0 0 0 0 0 0 17 0 0 0 0 0 14 70 0 0 0 0.42 0.10 85 F -2 -3 -4 -4 -2 -3 -3 -4 -3 1 4 -3 1 4 -4 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 74 0 0 26 0 0 0 0 0 0 0.52 0.10 86 S 0 4 0 -1 -2 0 0 -1 -1 -3 -3 1 -2 -3 -2 3 0 -3 -2 -2 0 45 0 0 0 0 0 0 0 0 0 0 0 0 0 55 0 0 0 0 0.49 0.10 87 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.55 0.10 88 N -2 -1 6 1 -3 0 -1 -1 0 -4 -4 0 -3 -3 -2 0 0 -4 -2 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.91 0.09 K Lambda Standard Ungapped 0.1241 0.3124 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1492 0.3166 PSI Gapped 0.0457 0.2670