Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 F -3 -4 -4 -5 -3 -4 -4 -4 -2 0 0 -4 0 8 -5 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.91 0.08 2 D -2 -3 0 6 -3 -1 1 -2 -2 0 -2 -2 -2 -3 -2 -1 -1 -5 -3 2 0 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 33 0.54 0.08 3 E -1 0 -1 2 -5 2 7 -3 0 -4 -4 1 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.08 4 Y -2 -2 -3 -4 -3 -2 -3 -4 2 -2 -2 -3 -2 3 -4 -2 -2 2 9 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.04 0.08 5 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 2 -4 1 0 -4 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.53 0.08 6 K -1 0 0 4 -3 0 1 -2 -2 -3 -3 3 -2 -4 -2 0 3 -4 -3 -2 0 0 0 33 0 0 0 0 0 0 0 39 0 0 0 0 28 0 0 0 0.40 0.08 7 E -1 0 -1 2 -5 2 7 -3 0 -4 -4 1 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.08 8 L -2 -3 -4 -5 -2 -3 -4 -5 -4 2 5 -3 2 0 -4 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.57 0.08 9 N -2 1 5 0 -4 1 0 -2 0 -4 -4 5 -2 -4 -2 0 -1 -4 -3 -3 0 0 39 0 0 0 0 0 0 0 0 61 0 0 0 0 0 0 0 0 0.53 0.08 10 E 4 -1 -1 0 -2 0 4 -1 -1 -3 -3 0 -2 -3 -1 1 -1 -4 -3 -1 61 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0.41 0.08 11 E -1 0 -1 2 -5 2 7 -3 0 -4 -4 1 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.08 12 R -1 6 -1 -2 -4 0 -1 4 -1 -4 -3 1 -2 -4 -3 -1 -2 -4 -3 -4 0 67 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0 0 0 0 0.64 0.08 13 D -2 -1 0 5 -5 1 5 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -4 0 0 0 39 0 0 61 0 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.08 14 I -1 -4 -4 -4 -1 -3 -4 -4 -4 4 1 -3 1 -1 -3 -3 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 61 0.45 0.08 15 L -2 -3 -4 -5 -2 -3 -4 -5 -4 2 5 -3 2 0 -4 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.57 0.08 16 N 0 -1 5 0 -2 -1 -1 -1 -1 -3 -3 -1 -2 -3 -2 3 3 -4 -3 -2 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 28 33 0 0 0 0.45 0.08 17 K -1 2 0 -1 -4 1 1 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.65 0.08 18 D -2 -2 1 8 -5 -1 2 -2 -2 -4 -5 -1 -4 -5 -2 -1 -2 -6 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.07 0.08 19 L -2 -4 -4 -4 -2 -3 -4 -5 -4 5 3 -3 2 0 -4 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 61 39 0 0 0 0 0 0 0 0 0 0.46 0.08 20 N -2 -1 8 1 -4 0 -1 -1 0 -4 -4 0 -3 -4 -3 0 0 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.87 0.08 21 K -1 2 0 -1 -4 1 1 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.65 0.08 22 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.52 0.08 23 L -2 -3 -4 -4 -2 -3 -4 -5 -4 4 3 -3 1 0 -4 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 33 39 0 0 0 0 0 0 0 0 28 0.41 0.08 24 K -1 2 0 -1 -4 1 1 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.65 0.08 25 D -2 -2 1 8 -5 -1 2 -2 -2 -4 -5 -1 -4 -5 -2 -1 -2 -6 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.07 0.08 26 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 2 -4 1 0 -4 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.53 0.08 27 E -1 0 -1 2 -5 2 7 -3 0 -4 -4 1 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.08 28 K -1 2 0 -1 -4 1 1 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.65 0.08 29 R -2 7 -1 -2 -4 1 0 -3 -1 -4 -3 2 -2 -4 -3 -1 -2 -4 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.89 0.08 30 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -5 9 -1 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.14 0.08 31 E -2 -1 0 5 -5 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -4 0 0 0 33 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.08 32 N -2 -1 6 1 -3 -1 -1 -1 0 -3 -3 -1 -2 -3 -2 0 -1 -3 -2 -3 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.35 0.08 33 K -1 2 -1 -1 -3 1 0 -2 -1 -3 -3 5 -2 -3 -1 -1 -1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0.22 0.08 34 K -1 2 -1 -1 -3 1 0 -2 -1 -3 -3 5 -2 -3 -1 -1 -1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0.22 0.08 35 A 4 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -2 -1 0 -1 -3 -2 -1 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.08 36 H -2 -1 -1 -2 -3 -1 -1 -3 7 -2 -2 -2 -2 1 -3 -2 -2 0 5 -2 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 28 0 0.31 0.08 37 N -2 -1 6 1 -3 -1 -1 -1 0 -3 -3 -1 -2 -3 -2 0 -1 -3 -2 -3 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.35 0.08 38 K -1 2 -1 -1 -3 1 0 -2 -1 -3 -3 5 -2 -3 -1 -1 -1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0.22 0.08 39 R -2 4 -1 -2 -2 0 -1 -2 -1 -2 -2 1 -2 -2 -2 -1 -1 -2 -2 -2 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.03 0.08 40 D -2 -2 0 5 -2 -1 0 -2 -1 -2 -2 -1 -2 -2 -2 -1 -1 -2 -2 -2 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.01 0.08 41 N -2 -1 5 0 -2 -1 -1 -1 -1 -2 -2 -1 -2 -2 -2 -1 -1 -2 -2 -2 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.03 0.08 42 L -2 -2 -2 -2 -2 -2 -2 -2 -2 0 3 -2 0 -1 -2 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 -0.13 0.08 43 Q -1 0 -1 -1 -2 4 0 -2 -1 -2 -2 0 -1 -2 -2 -1 -1 -2 -2 -2 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.05 0.08 44 Q -1 0 -1 -1 -2 4 0 -2 -1 -2 -2 0 -1 -2 -2 -1 -1 -2 -2 -2 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.05 0.08 45 Q -1 0 -1 -1 -2 4 0 -2 -1 -2 -2 0 -1 -2 -2 -1 -1 -2 -2 -2 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.05 0.08 46 L -2 -2 -2 -2 -2 -2 -2 -2 -2 0 3 -2 0 -1 -2 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 -0.13 0.08 47 D -2 -2 0 5 -2 -1 0 -2 -1 -2 -2 -1 -2 -2 -2 -1 -1 -2 -2 -2 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.01 0.08 48 A 3 -2 -2 -2 -1 -1 -1 -1 -2 -2 -2 -1 -1 -2 -1 0 -1 -2 -2 -1 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.12 0.08 49 N -2 -1 5 0 -2 -1 -1 -1 -1 -2 -2 -1 -2 -2 -2 -1 -1 -2 -2 -2 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.03 0.08 50 E -1 -1 -1 0 -2 0 4 -2 -1 -2 -2 0 -2 -2 -1 -1 -1 -2 -2 -2 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.04 0.08 51 Q -1 0 -1 -1 -2 4 0 -2 -1 -2 -2 0 -1 -2 -2 -1 -1 -2 -2 -2 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.05 0.08 52 K -1 1 -1 -1 -2 0 0 -2 -1 -2 -2 3 -2 -2 -1 -1 -1 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 -0.10 0.08 53 I -2 -2 -2 -2 -1 -2 -2 -2 -2 3 0 -2 0 -1 -2 -2 -1 -2 -2 1 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 -0.15 0.08 54 E -1 -1 -1 0 -2 0 4 -2 -1 -2 -2 0 -2 -2 -1 -1 -1 -2 -2 -2 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.04 0.08 55 E -1 -1 -1 0 -2 0 4 -2 -1 -2 -2 0 -2 -2 -1 -1 -1 -2 -2 -2 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.04 0.08 56 G -1 -2 -1 -2 -2 -2 -2 4 -2 -2 -2 -2 -2 -2 -2 -1 -2 -2 -2 -2 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0.03 0.08 57 K -1 1 -1 -1 -2 0 0 -2 -1 -2 -2 3 -2 -2 -1 -1 -1 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 -0.10 0.08 58 R -2 4 -1 -2 -2 0 -1 -2 -1 -2 -2 1 -2 -2 -2 -1 -1 -2 -2 -2 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.03 0.08 59 L -2 -2 -2 -2 -2 -2 -2 -2 -2 0 3 -2 0 -1 -2 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 -0.13 0.08 60 Q -1 1 0 -1 -4 6 2 -2 0 -4 -3 4 -1 -4 -2 0 -1 -3 -2 -3 0 0 0 0 0 67 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0.63 0.08 61 E 2 -1 -1 1 -3 1 5 -2 -1 -3 -3 0 -2 -4 -2 0 -1 -4 -3 -2 33 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0.53 0.08 62 E -1 2 -1 0 -4 2 4 -2 -1 -4 -3 5 -2 -4 -2 0 -1 -4 -3 -3 0 0 0 0 0 0 39 0 0 0 0 61 0 0 0 0 0 0 0 0 0.57 0.08 63 H -2 -1 0 -2 -4 0 0 -3 10 -4 -4 -1 -2 -2 -3 -1 -2 -3 2 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 1.07 0.08 64 G 0 -3 -1 -2 -3 -2 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.16 0.08 65 N -2 -1 8 1 -4 0 -1 -1 0 -4 -4 0 -3 -4 -3 0 0 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.87 0.08 K Lambda Standard Ungapped 0.1287 0.3043 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1533 0.3179 PSI Gapped 0.0469 0.2670