Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -2 -2 -3 -4 -2 -2 -3 -4 -3 1 1 -2 8 -1 -3 -2 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 4 0 0 82 0 0 0 0 0 0 14 0.86 0.29 2 T -1 -1 4 -1 -2 -1 -1 -2 -2 -2 -3 0 -2 -3 -2 0 5 -4 -3 -2 0 0 34 0 0 0 0 0 0 0 0 6 0 0 0 0 60 0 0 0 0.69 0.29 3 K -2 3 -1 -2 -4 1 0 -3 -1 -4 -3 6 -2 -4 -2 -1 -2 -4 -3 -3 0 17 0 0 0 0 0 0 0 0 0 83 0 0 0 0 0 0 0 0 0.91 0.29 4 Q -2 0 -1 -1 -4 7 1 -3 0 -3 -2 0 -1 -4 0 -1 -2 -3 -2 -3 0 0 0 0 0 94 0 0 0 0 3 0 0 0 3 0 0 0 0 0 1.05 0.29 5 E -2 0 -1 0 -4 5 5 -3 -1 -4 -4 0 -2 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 34 66 0 0 0 0 0 0 0 0 0 0 0 0 0 0.96 0.29 6 K 0 1 -1 -1 -3 3 0 -2 -1 -3 -3 5 -2 -4 -2 2 0 -4 -3 -3 6 0 0 0 0 14 0 0 0 0 0 58 0 0 0 19 3 0 0 0 0.59 0.29 7 T 3 -2 -1 -2 -2 -2 -2 -2 -3 -2 -2 -2 0 -3 -2 1 5 -3 -3 -1 35 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 62 0 0 0 0.61 0.29 8 A 5 -2 -2 -3 -1 -2 -2 -1 -3 -1 -2 -2 -1 -3 -2 0 1 -4 -3 2 71 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11 0 0 18 0.54 0.29 9 L -2 -3 -4 -5 -2 -3 -4 -5 -4 2 5 -3 1 0 -4 -3 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 6 85 0 0 0 0 0 0 0 0 10 0.75 0.29 10 N -2 -1 7 1 -4 -1 -1 -1 0 -4 -4 -1 -3 -4 -3 0 -1 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.26 0.29 11 M -2 -3 -3 -4 -2 -2 -3 -4 -3 1 1 -2 9 -1 -4 -3 -2 -3 -2 0 0 0 0 0 0 0 0 0 0 5 0 0 95 0 0 0 0 0 0 0 1.14 0.36 12 A 5 -3 -2 -1 -2 -2 -2 -1 -3 -2 -2 -2 -2 -3 -2 0 0 -4 -3 1 80 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 5 0 0 12 0.65 0.36 13 R -2 3 -1 -2 4 0 -1 -1 -2 -4 -3 5 -2 -4 -2 -1 -2 -4 -3 -3 0 19 0 0 12 0 0 4 0 0 0 66 0 0 0 0 0 0 0 0 0.79 0.36 14 F -3 -4 -4 -5 -4 -4 -4 -4 -2 -1 -1 -4 -1 8 -5 -4 -3 0 2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.47 0.36 15 I -2 -4 -5 -4 -2 -4 -4 -5 -4 6 2 -4 0 -1 -4 -4 -2 -4 -2 2 0 0 0 0 0 0 0 0 0 86 14 0 0 0 0 0 0 0 0 0 0.93 0.39 16 R -2 5 -1 -2 -4 5 0 -3 -1 -4 -3 3 -2 -4 -3 -1 -2 -4 -3 -4 0 45 0 0 0 33 0 0 0 0 0 22 0 0 0 0 0 0 0 0 0.91 0.40 17 S 3 -2 2 1 -2 -1 0 1 0 -3 -4 -2 -3 -4 -2 3 0 -4 -3 -3 32 0 13 8 0 0 3 8 2 0 0 0 0 0 0 31 3 0 0 0 0.44 0.40 18 Q -2 3 -1 -2 -4 7 1 -3 -1 -4 -3 0 -2 -4 -3 -1 -2 -3 -3 -3 0 14 0 0 0 86 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.17 0.40 19 T -1 -2 -1 -2 -2 -2 -2 -2 -3 1 -1 -2 -2 -3 -2 4 4 -4 -3 -1 0 0 0 0 0 0 0 0 0 12 4 0 0 0 0 45 40 0 0 0 0.59 0.40 20 L -1 -2 -2 -2 -2 -2 -2 -2 -2 0 4 -2 0 -1 2 -2 -1 -2 -1 0 3 2 2 2 1 1 2 3 1 2 57 2 1 1 13 2 2 0 1 2 0.39 0.26 21 T -1 -2 -1 -2 -1 -1 -2 -2 -2 1 2 -1 0 -1 -1 -1 3 -2 -1 1 3 2 2 2 1 1 2 3 1 5 27 2 1 1 2 2 29 0 1 14 0.23 0.26 22 L -3 -3 -4 -5 -2 -1 -4 -5 -4 0 5 -3 1 -1 -4 -4 -2 -3 -2 0 0 0 0 0 0 4 0 0 0 0 96 0 0 0 0 0 0 0 0 0 0.92 0.40 23 L -3 -3 -4 -5 -3 -3 -4 -5 -4 0 5 -3 1 -1 2 -3 -2 -3 -2 0 0 0 0 0 0 0 0 0 0 0 88 0 0 0 12 0 0 0 0 0 0.86 0.40 24 E -2 -2 -2 -1 -4 0 5 -4 -2 -1 3 -1 -1 -2 -3 -2 -2 -4 -3 -2 0 0 0 0 0 0 59 0 0 0 41 0 0 0 0 0 0 0 0 0 0.69 0.40 25 K -2 1 -2 -2 -4 0 1 -3 -2 -2 2 5 -1 -3 -3 -2 -2 -4 -3 -2 0 5 0 0 0 0 7 0 0 0 34 54 0 0 0 0 0 0 0 0 0.55 0.40 26 L -2 -2 -3 1 4 -1 3 -4 -3 0 3 0 0 -2 -3 -2 -2 -3 -3 -1 0 0 0 8 12 0 26 0 0 3 44 7 0 0 0 0 0 0 0 0 0.42 0.40 27 N -2 1 4 2 -4 0 1 -3 4 -4 -1 3 -3 -4 -3 -1 -2 -4 -2 -3 0 6 27 15 0 0 5 0 12 0 7 28 0 0 0 0 0 0 0 0 0.52 0.40 28 E 0 -2 1 -1 -3 2 4 -3 -2 0 2 -1 -1 -2 -3 -2 -2 -3 -3 -2 8 0 7 0 0 9 39 0 0 4 31 0 0 0 0 0 0 0 0 0 0.39 0.38 29 L -2 -2 4 1 -3 -1 0 -2 -2 -1 3 -2 2 -2 -2 -1 -1 -3 -2 -1 1 1 26 10 0 1 8 1 0 1 39 1 6 1 1 1 1 0 0 1 0.34 0.34 30 A 0 -1 -1 1 -1 0 3 -1 -1 -1 -1 0 -1 -1 -1 1 -1 -1 -1 0 7 2 2 6 1 2 28 4 1 2 8 3 1 2 2 14 3 1 2 9 0.17 0.21 31 D -1 -1 0 1 -2 2 -1 -2 -1 0 3 -1 0 -1 -1 -1 -1 -2 -1 -1 3 2 6 12 1 13 2 3 1 2 42 2 1 1 2 3 2 0 1 2 0.21 0.25 32 A -2 -2 4 6 -4 -1 1 -2 -2 -4 -5 0 -4 -5 -3 -1 -2 -5 -4 -4 3 0 27 61 0 0 2 0 0 0 0 7 0 0 0 0 0 0 0 0 1.01 0.40 33 A 3 -3 -4 -4 -2 -3 -3 -3 -3 0 4 -3 2 1 -3 -2 -2 -3 -2 -1 38 0 0 0 0 0 0 0 0 0 50 0 5 6 0 0 0 0 0 0 0.51 0.40 34 D -3 -3 0 7 -5 -1 1 -2 -2 -4 -5 -2 -4 -5 -3 -1 -2 -6 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.56 0.40 35 E 2 -2 -1 4 -3 -1 4 -1 0 -2 -3 -1 -3 -4 -2 -1 0 -4 -3 -1 21 0 0 29 0 0 34 3 3 2 0 0 0 0 0 0 5 0 0 3 0.54 0.40 36 Q -1 0 -1 -1 -4 4 4 -1 4 -1 -3 0 0 -4 0 -1 -2 -4 -2 -3 5 4 0 0 0 27 38 3 12 4 0 0 3 0 5 0 0 0 0 0 0.57 0.40 37 A 6 -3 -2 -3 -2 -2 -2 -1 -3 -2 -3 -2 -2 -3 -2 0 1 -4 -3 -1 89 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11 0 0 0 0.83 0.40 38 D 1 -2 3 6 -4 -1 0 0 -2 -4 -4 -2 -3 -4 -3 -1 1 -5 -4 -3 14 0 19 52 0 0 0 5 0 0 0 0 0 0 0 0 10 0 0 0 0.75 0.40 39 I -2 -3 -3 0 2 -1 0 -4 -3 3 2 -3 5 0 -4 -3 -2 -3 0 0 0 0 0 7 5 3 9 0 0 27 19 0 23 3 0 0 0 0 4 0 0.38 0.40 40 C -1 -5 -4 -5 10 -4 -5 -4 -4 -2 -2 -4 -3 -4 -4 -2 -2 -4 -4 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.20 0.40 41 E -2 -1 -1 3 -5 1 6 -3 -1 -4 -4 0 -3 -5 -2 -1 -2 -4 -3 -4 0 0 0 12 0 0 88 0 0 0 0 0 0 0 0 0 0 0 0 0 1.18 0.40 42 S -1 3 -1 -2 -4 2 1 -3 2 -4 -4 4 -3 -4 2 1 -1 -4 -3 -3 2 19 0 0 0 7 10 0 5 0 0 33 0 0 12 14 0 0 0 0 0.54 0.39 43 L -3 -3 -4 -5 -3 -3 -4 -5 -4 0 5 -3 2 -1 2 -3 -2 -3 -2 0 0 0 0 0 0 0 0 0 0 0 86 0 3 0 12 0 0 0 0 0 0.84 0.39 44 H -3 -1 0 -2 -4 -1 -1 -3 10 -5 -4 -2 -3 -2 -3 -2 -3 -4 1 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 1.80 0.39 45 D -2 -2 2 4 -5 0 5 -3 -1 -4 -4 -1 -3 -5 -2 -1 -2 -5 -3 -4 0 0 12 27 0 0 62 0 0 0 0 0 0 0 0 0 0 0 0 0 0.97 0.39 46 H -3 -2 -1 3 -4 3 0 -3 6 -2 1 -1 -1 -3 -3 -2 -2 -4 -1 -3 0 0 0 22 0 22 0 0 34 0 22 0 0 0 0 0 0 0 0 0 0.64 0.39 47 A 6 -3 -2 -3 -2 -2 -2 -1 -3 -3 -3 -2 -2 -3 -2 1 -1 -4 -3 -1 93 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0.88 0.39 48 D -2 -2 -1 4 -5 0 6 -3 -1 -4 -4 -1 -3 -5 -2 -1 -2 -4 -3 -4 0 0 0 28 0 0 72 0 0 0 0 0 0 0 0 0 0 0 0 0 1.10 0.39 49 E 1 1 -1 -1 -3 3 4 -2 1 -3 -2 1 0 -4 -2 1 -1 -4 -3 -2 13 5 0 0 0 14 38 2 4 0 2 5 2 0 0 10 3 0 0 2 0.42 0.39 50 L -3 -3 -5 -5 -2 -3 -4 -5 -4 1 5 -4 1 -1 -4 -4 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 0 94 0 0 0 0 0 0 0 0 6 0.92 0.38 51 Y -3 -2 -2 -3 3 2 -2 -4 6 -3 -2 -2 -2 3 -4 -2 -3 4 6 -3 0 0 0 0 7 11 0 0 23 0 0 0 0 9 0 0 0 5 45 0 0.93 0.39 52 R -1 6 2 0 -4 3 0 -3 -1 -4 -2 1 -2 -4 -3 -1 -2 -4 -3 -3 5 53 10 3 0 20 5 0 0 0 3 2 0 0 0 0 0 0 0 0 0.73 0.42 53 S -1 0 -1 0 -3 1 -1 0 1 -3 -3 2 1 -3 -2 4 2 -4 -3 -1 0 2 0 5 0 6 0 5 3 0 0 17 5 0 0 39 13 0 0 6 0.34 0.42 54 C -2 -4 -4 -4 4 -3 -4 1 -4 2 4 -4 2 -1 -4 -3 -2 -3 -3 0 0 0 0 0 12 0 0 13 0 12 55 0 6 0 0 0 0 0 0 2 0.57 0.42 55 L -1 3 0 -2 -3 2 -2 -3 -2 -1 1 -1 -1 2 -3 2 0 -3 0 -2 2 19 5 0 0 12 0 0 0 2 19 0 0 11 0 23 5 0 3 0 0.25 0.42 56 A 4 -3 -1 -1 -2 -2 -1 1 -3 0 -2 -2 -2 -3 -2 0 1 -4 -3 -1 58 0 3 4 0 0 4 8 0 5 2 0 0 0 0 2 11 0 0 2 0.44 0.42 57 R -2 5 -2 -3 -4 2 -1 -3 2 -4 -3 3 -2 -2 -3 -2 -1 -3 2 -3 0 50 0 0 0 10 0 0 5 0 0 20 0 0 0 0 4 0 11 0 0.77 0.42 58 F -3 -4 -5 -5 -3 -4 -4 -5 -3 2 4 -4 0 6 -4 -4 -3 -2 0 1 0 0 0 0 0 0 0 0 0 9 45 0 0 40 0 0 0 0 0 6 0.78 0.42 59 G -1 -3 2 -2 -4 -2 -3 6 -2 -5 -5 -2 -4 -4 -3 -1 -2 -4 -4 -4 0 0 15 0 0 0 0 85 0 0 0 0 0 0 0 0 0 0 0 0 1.21 0.29 K Lambda Standard Ungapped 0.1292 0.3189 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1679 0.3190 PSI Gapped 0.0514 0.2670