Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -2 -2 -3 -4 -2 -1 -3 -4 -2 1 2 -2 9 0 -3 -2 -1 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.72 0.16 2 N -2 -1 8 1 -4 0 -1 -1 0 -4 -5 -1 -3 -4 -3 0 -1 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.02 0.16 3 Q -2 0 4 0 -4 7 1 -2 0 -4 -3 1 -1 -4 -2 0 -1 -3 -2 -3 0 0 26 0 0 74 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.74 0.16 4 G 2 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 0 -2 -4 -4 -3 26 0 0 0 0 0 0 74 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.16 5 K -2 4 -1 -2 -4 1 0 -3 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 22 0 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0.72 0.16 6 I -2 -3 -4 -4 -2 -3 -4 -5 -4 5 1 -3 5 -1 -4 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 74 0 0 26 0 0 0 0 0 0 0 0.55 0.16 7 W -4 -4 -5 -6 -3 -3 -4 -4 -3 -4 -2 -4 -2 1 -5 -4 -3 13 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.70 0.16 8 T -1 -3 -2 -3 6 -2 -3 -3 -3 -1 2 -2 0 -2 -3 0 5 -3 -2 0 0 0 0 0 22 0 0 0 0 0 26 0 0 0 0 0 52 0 0 0 0.46 0.16 9 V -1 -3 -3 -3 -2 -2 -3 -3 -3 1 0 -3 3 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 0 0 0 15 0 0 0 0 0 0 55 0.14 0.18 10 V -1 -3 -3 -3 -2 -3 -3 -3 -3 2 0 -3 0 -2 -3 -2 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 69 0.20 0.18 11 N -2 0 4 -1 -3 0 -1 -2 4 -3 -3 2 -2 -3 -2 -1 -1 -3 -1 -3 0 0 33 0 0 0 0 0 15 0 0 22 0 0 0 0 0 0 0 0 0.20 0.18 12 P -2 -3 -2 -2 -3 -2 -2 -3 -3 -3 -3 -2 -3 -4 8 -2 -2 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 69 0 0 0 0 0 1.21 0.18 13 A 2 -2 -1 -2 -1 -1 -2 -2 -2 -2 -2 -1 -1 -3 -2 0 5 -3 -2 -1 19 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 0 0 0 0.18 0.18 14 I -1 -3 -3 -3 -2 -3 -3 -3 -3 3 0 -3 0 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 19 0 0 0 0 0 0 0 0 0 51 0.17 0.18 15 G -1 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -3 -3 -1 -2 -3 -3 -3 0 0 0 0 0 0 0 69 0 0 0 0 0 0 0 0 0 0 0 0 0.61 0.18 16 I -2 -3 -3 -3 -2 -3 -3 -3 -3 3 3 -3 0 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 19 29 0 0 0 0 0 0 0 0 22 0.11 0.18 17 P -2 -3 -2 -2 -3 -2 -2 -3 -3 -3 -3 -2 -3 -4 8 -2 -2 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 69 0 0 0 0 0 1.21 0.18 18 A 1 -2 -3 -3 -2 -2 -3 -3 -3 0 4 -2 1 -1 -3 -2 -2 -2 -2 0 19 0 0 0 0 0 0 0 0 0 51 0 0 0 0 0 0 0 0 0 0.09 0.18 19 L -2 -3 -3 -3 -3 -3 -3 -3 -2 0 2 -3 0 6 -3 -3 -2 0 1 -1 0 0 0 0 0 0 0 0 0 0 19 0 0 51 0 0 0 0 0 0 0.31 0.18 20 L -2 -3 -3 -4 -2 -3 -3 -4 -3 1 4 -3 1 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 69 0 0 0 0 0 0 0 0 0 0.24 0.18 21 G -1 -2 -1 -2 -2 -2 -2 5 -2 -3 -3 -2 -2 -3 -2 -1 2 -3 -3 -2 0 0 0 0 0 0 0 47 0 0 0 0 0 0 0 0 22 0 0 0 0.32 0.18 22 S 2 -2 -1 -1 -1 -1 -1 -1 -2 -2 -3 -1 -2 -3 -2 4 0 -3 -2 -2 22 0 0 0 0 0 0 0 0 0 0 0 0 0 0 47 0 0 0 0 0.16 0.18 23 V -1 -3 -3 -3 -2 -3 -3 -3 -3 2 0 -3 0 -2 -3 -2 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 69 0.20 0.18 24 T 4 -2 -2 -2 -1 -2 -2 -1 -2 -2 -2 -1 -2 -3 -2 0 2 -3 -2 -1 51 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 19 0 0 0 0.14 0.18 25 V -1 -3 -3 -3 -2 -3 -3 -3 -3 1 2 -3 0 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 0 29 0 0 0 0 0 0 0 0 41 0.12 0.18 26 I -1 -2 -2 -2 -2 -2 -2 3 -3 2 -1 -2 -1 -2 -2 -1 3 -3 -2 0 0 0 0 0 0 0 0 22 0 19 0 0 0 0 0 0 29 0 0 0 0.06 0.18 27 A 2 -2 -1 -1 -1 -1 -1 -1 -2 -2 -3 -1 -2 -3 -2 4 0 -3 -2 -2 19 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 0 0 0 0 0.17 0.18 28 I -2 -3 -3 -3 -2 -3 -3 -3 -3 2 3 -3 0 4 -3 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 19 29 0 0 22 0 0 0 0 0 0 0.13 0.18 29 L 2 -3 -3 -3 7 -2 -3 -2 -3 -1 1 -2 -1 -2 -2 -1 -1 -3 -2 -1 22 0 0 0 29 0 0 0 0 0 19 0 0 0 0 0 0 0 0 0 0.20 0.18 30 V -1 -3 -3 -3 -2 -3 -3 -3 -3 2 0 -3 0 -2 -3 -2 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 69 0.20 0.18 31 H -2 -1 0 -2 -3 0 -1 -3 9 -3 -3 -1 -2 -2 -3 -2 -2 -3 1 -3 0 0 0 0 0 0 0 0 69 0 0 0 0 0 0 0 0 0 0 0 0.65 0.18 32 L -2 -3 -3 -3 -2 -2 -3 -3 -1 0 1 -3 0 1 -3 -2 -2 0 5 2 0 0 0 0 0 0 0 0 0 0 19 0 0 0 0 0 0 0 29 22 0.13 0.18 33 A 2 -2 -1 -2 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 -2 2 3 -3 -2 -1 19 0 0 0 0 0 0 0 0 0 0 0 0 0 0 22 29 0 0 0 0.11 0.18 34 I -1 -3 -3 -3 -2 -3 -3 -3 -3 4 0 -3 0 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0 0 0 0 0 36 0.17 0.18 35 L -2 -3 -3 -4 -2 -3 -3 -4 -3 1 4 -3 1 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 69 0 0 0 0 0 0 0 0 0 0.24 0.18 36 S 0 3 -1 -1 -2 -1 -1 -2 -1 -3 -3 0 -2 -3 -2 4 0 -3 -2 -2 0 22 0 0 0 0 0 0 0 0 0 0 0 0 0 47 0 0 0 0 0.16 0.18 37 H -2 -1 4 -1 -4 0 -1 -2 9 -4 -4 -1 -3 -2 -3 -1 -2 -4 1 -4 0 0 22 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0 0 0 1.06 0.18 38 T -1 -2 -1 -2 -2 -1 -2 -2 -3 -1 -2 -1 -1 -3 -2 1 7 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.76 0.18 39 T -1 -2 -1 -2 -2 -1 -2 -2 -3 -1 -2 -1 -1 -3 -2 1 7 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.76 0.18 40 W -4 -4 -5 -6 -3 -3 -4 -4 -3 -4 -3 -4 -2 1 -5 -4 -3 13 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.75 0.18 41 F -3 -3 -4 -5 -3 -3 -4 -4 -2 0 2 -3 5 6 -4 -3 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 22 0 29 49 0 0 0 0 0 0 0.62 0.18 42 P -1 -3 -2 -2 -4 -2 -2 3 -3 -4 -4 -2 -4 -5 8 -1 -2 -5 -4 -4 0 0 0 0 0 0 0 29 0 0 0 0 0 0 71 0 0 0 0 0 1.55 0.18 43 A 5 -1 -2 -2 -2 3 -1 -1 -2 -2 -2 -1 -2 -3 -2 1 -1 -3 -3 -1 78 0 0 0 0 22 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.52 0.18 44 Y -3 -3 -3 -4 -3 -3 -3 -4 1 -2 -1 -3 -1 5 -4 -3 -3 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 22 0 0 0 0 78 0 1.09 0.18 45 W -3 -4 -5 -5 -3 -3 -4 -4 -3 -2 1 -4 -1 0 -5 -4 -3 12 1 -2 0 0 0 0 0 0 0 0 0 0 22 0 0 0 0 0 0 78 0 0 2.00 0.18 46 Q -1 0 0 0 -4 6 4 -2 -1 -4 -3 0 -2 -4 -2 2 -1 -3 -3 -3 0 0 0 0 0 49 29 0 0 0 0 0 0 0 0 22 0 0 0 0 0.63 0.18 47 G 0 -3 -1 -2 -4 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -4 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.31 0.18 48 G 0 -3 -1 -2 -4 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -4 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.31 0.18 49 V -1 0 -2 -3 -2 -1 -1 -3 -3 1 2 3 0 -2 -3 -2 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 0 35 40 0 0 0 0 0 0 0 25 0.25 0.13 50 K 0 1 0 -1 -3 1 0 -2 -1 -3 -3 5 -2 -4 -2 3 0 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 60 0 0 0 40 0 0 0 0 0.49 0.13 51 K -1 2 -1 -1 -4 1 1 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.72 0.13 52 A 4 0 -2 -2 -2 0 -1 -1 -2 -3 -3 4 -2 -3 -1 0 -1 -4 -3 -1 60 0 0 0 0 0 0 0 0 0 0 40 0 0 0 0 0 0 0 0 0.41 0.13 53 A 6 -2 -2 -3 -1 -1 -1 0 -2 -2 -2 -1 -2 -3 -1 1 0 -3 -3 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.59 0.13 K Lambda Standard Ungapped 0.1363 0.3235 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1597 0.3175 PSI Gapped 0.0489 0.2670