Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -2 -3 -4 -5 -2 -2 -3 -4 -3 1 4 -3 6 0 -4 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 0 61 0 39 0 0 0 0 0 0 0 0.47 0.07 2 A 6 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.51 0.07 3 K -1 2 0 -1 -4 1 1 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.64 0.07 4 E -1 0 -1 2 -5 2 7 -3 0 -4 -4 1 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.07 5 F -3 -4 -4 -5 -3 -4 -4 -4 -2 0 0 -4 0 8 -5 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.90 0.07 6 G 0 -3 -1 -2 -3 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.16 0.07 7 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 2 -4 1 0 -4 -3 -1 -4 -2 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.52 0.07 8 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -5 9 -1 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.13 0.07 9 A 5 -2 -2 -2 -1 -2 -2 4 -2 -3 -3 -1 -2 -3 -2 1 -1 -3 -3 -1 71 0 0 0 0 0 0 29 0 0 0 0 0 0 0 0 0 0 0 0 0.49 0.07 10 A 3 -2 0 -1 -1 -1 -1 0 -2 -3 -3 -1 -2 -3 -1 4 1 -4 -2 -2 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 61 0 0 0 0 0.43 0.07 11 V -1 -4 -4 -4 -1 -3 -4 -4 -4 4 1 -3 1 -1 -3 -3 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 32 0 0 0 0 0 0 0 0 0 68 0.44 0.07 12 A 6 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.51 0.07 13 G 4 -2 -2 -2 -2 -2 -2 5 -3 -3 -3 -2 -2 -3 -2 1 -1 -3 -3 -2 61 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0.53 0.07 14 T 0 -3 -2 -3 -1 -2 -2 -3 -3 2 0 -2 0 -2 -3 0 4 -4 -2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 0 0 61 0.33 0.07 15 V -1 -3 -4 -4 -1 -3 -3 -4 -4 3 1 -3 1 -1 -3 -2 0 -4 -2 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.47 0.07 16 L -2 -3 -5 -5 -2 -3 -4 -5 -4 2 5 -3 2 0 -4 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.57 0.07 17 N -2 -1 8 1 -4 0 -1 -1 0 -4 -5 0 -3 -4 -3 0 0 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.87 0.07 18 V -1 -4 -4 -4 -1 -3 -4 -4 -4 4 1 -3 1 -1 -3 -3 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 32 0 0 0 0 0 0 0 0 0 68 0.44 0.07 19 V -1 -3 -4 -4 -1 -3 -3 -4 -4 3 1 -3 1 -1 -3 -2 0 -4 -2 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.47 0.07 20 E -2 -1 4 2 -4 1 5 -2 0 -4 -4 0 -3 -4 -2 0 -1 -4 -3 -4 0 0 32 0 0 0 68 0 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.07 21 A 4 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -2 -1 1 -1 -3 -2 -1 68 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.07 22 G 0 -2 -1 -2 -3 -2 -2 6 -2 -3 -3 -2 -3 -3 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 0 68 0 0 0 0 0 0 0 0 0 0 0 0 0.52 0.07 23 G 0 -2 -1 -2 -3 -2 -2 6 -2 -3 -3 -2 -3 -3 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 0 68 0 0 0 0 0 0 0 0 0 0 0 0 0.52 0.07 24 W -2 -2 -2 -2 -2 -2 -2 -2 -2 -2 -2 -2 -2 0 -2 -2 -2 10 1 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0.46 0.07 25 V -1 -2 -2 -2 -1 -2 -2 -2 -2 1 0 -2 0 -1 -2 -2 -1 -2 -1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 -0.15 0.07 26 T -1 -1 -1 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 0 4 -2 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 -0.10 0.07 27 T -1 -1 -1 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 0 4 -2 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 -0.10 0.07 28 I -2 -2 -2 -2 -2 -2 -2 -2 -2 3 0 -2 0 -1 -2 -2 -1 -2 -2 1 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 -0.15 0.07 29 V -1 -2 -2 -2 -1 -2 -2 -2 -2 1 0 -2 0 -1 -2 -2 -1 -2 -1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 -0.15 0.07 30 S 0 -1 0 -1 -1 -1 -1 -1 -1 -2 -2 -1 -2 -2 -1 3 0 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 -0.11 0.07 31 I -2 -2 -2 -2 -2 -2 -2 -2 -2 3 0 -2 0 -1 -2 -2 -1 -2 -2 1 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 -0.15 0.07 32 L -2 -2 -2 -2 -2 -2 -2 -2 -2 0 3 -2 1 -1 -2 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 -0.13 0.07 33 T -1 -1 -1 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 0 4 -2 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 -0.10 0.07 34 A 3 -2 -2 -2 -1 -1 -1 -1 -2 -2 -2 -1 -1 -2 -1 0 -1 -2 -2 -1 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.12 0.07 35 V -1 -2 -2 -2 -1 -2 -2 -2 -2 1 0 -2 0 -1 -2 -2 -1 -2 -1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 -0.15 0.07 36 G -1 -2 -1 -2 -2 -2 -2 4 -2 -2 -2 -2 -2 -2 -2 -1 -2 -2 -2 -2 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0.03 0.07 37 S 0 -1 0 -1 -1 -1 -1 -1 -1 -2 -2 -1 -2 -2 -1 3 0 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 -0.11 0.07 38 G -1 -2 -1 -2 -2 -2 -2 4 -2 -2 -2 -2 -2 -2 -2 -1 -2 -2 -2 -2 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0.03 0.07 39 G -1 -2 -1 -2 -2 -2 -2 4 -2 -2 -2 -2 -2 -2 -2 -1 -2 -2 -2 -2 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0.03 0.07 40 L -2 -2 -2 -2 -2 -2 -2 -2 -2 0 3 -2 1 -1 -2 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 -0.13 0.07 41 S 0 -1 0 -1 -1 -1 -1 -1 -1 -2 -2 -1 -2 -2 -1 3 0 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 -0.11 0.07 42 L -2 -2 -2 -2 -2 -2 -2 -2 -2 0 3 -2 1 -1 -2 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 -0.13 0.07 43 L -2 -2 -2 -2 -2 -2 -2 -2 -2 0 3 -2 1 -1 -2 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 -0.13 0.07 44 A 3 -2 -2 -2 -1 -1 -1 -1 -2 -2 -2 -1 -1 -2 -1 0 -1 -2 -2 -1 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.12 0.07 45 A 3 -2 -2 -2 -1 -1 -1 -1 -2 -2 -2 -1 -1 -2 -1 0 -1 -2 -2 -1 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.12 0.07 46 A 3 -2 -2 -2 -1 -1 -1 -1 -2 -2 -2 -1 -1 -2 -1 0 -1 -2 -2 -1 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.12 0.07 47 G -1 -2 -1 -2 -2 -2 -2 4 -2 -2 -2 -2 -2 -2 -2 -1 -2 -2 -2 -2 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 0 0.03 0.07 48 R -2 5 -1 -2 -4 1 0 -3 -1 -4 -3 5 -2 -4 -2 -1 -1 -4 -3 -3 0 39 0 0 0 0 0 0 0 0 0 61 0 0 0 0 0 0 0 0 0.63 0.07 49 E -1 0 -1 2 -5 2 7 -3 0 -4 -4 1 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.07 50 S 0 -2 0 -1 -1 -1 -1 -2 -2 -2 -2 -1 -1 -3 -1 4 5 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 39 61 0 0 0 0.48 0.07 51 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 2 -4 1 0 -4 -3 -1 -4 -2 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.52 0.07 52 K -2 6 -1 -2 -4 1 0 -3 -1 -4 -3 4 -2 -4 -2 -1 -1 -4 -2 -3 0 61 0 0 0 0 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0.68 0.07 53 A 3 0 -1 -1 -2 6 1 -1 -1 -3 -3 1 -1 -4 -2 0 -1 -3 -2 -2 39 0 0 0 0 61 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.46 0.07 54 Y -3 -3 -3 -4 -3 -3 -3 -4 1 -1 -1 -3 -1 6 -4 -3 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 32 0 0 0 0 68 0 0.87 0.07 55 L -2 -3 -5 -5 -2 -3 -4 -5 -4 2 5 -3 2 0 -4 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.57 0.07 56 K -1 2 0 -1 -4 1 1 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.64 0.07 57 K -1 2 4 0 -4 1 0 -2 -1 -4 -4 5 -2 -4 -2 0 -1 -4 -3 -3 0 0 29 0 0 0 0 0 0 0 0 71 0 0 0 0 0 0 0 0 0.52 0.07 58 E -1 1 -1 1 -5 2 6 -3 -1 -4 -4 4 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 0 0 0 68 0 0 0 0 32 0 0 0 0 0 0 0 0 0.64 0.07 59 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 2 -4 1 0 -4 -3 -1 -4 -2 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.52 0.07 60 K -1 2 0 -1 -4 4 1 -2 -1 -4 -3 5 -2 -4 -2 0 -1 -4 -2 -3 0 0 0 0 0 32 0 0 0 0 0 68 0 0 0 0 0 0 0 0 0.57 0.07 61 K -1 2 -1 0 -4 2 4 -2 -1 -4 -4 5 -2 -4 -2 0 -1 -4 -3 -3 0 0 0 0 0 0 32 0 0 0 0 68 0 0 0 0 0 0 0 0 0.56 0.07 62 K -1 2 0 -1 -4 1 1 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.64 0.07 63 G 0 -3 -1 -2 -3 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.16 0.07 64 K -1 5 -1 -1 -4 1 0 -2 -1 -4 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 29 0 0 0 0 0 0 0 0 0 71 0 0 0 0 0 0 0 0 0.62 0.07 65 R -2 6 -1 -2 -4 1 0 -3 -1 -4 -3 4 -2 -4 -2 -1 -2 -4 -2 -3 0 71 0 0 0 0 0 0 0 0 0 29 0 0 0 0 0 0 0 0 0.72 0.07 66 A 6 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.51 0.07 67 V 0 -3 -2 -3 -1 -2 -3 -3 -4 2 0 -2 0 -2 -3 -1 3 -4 -2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 32 0 0 68 0.34 0.07 68 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 2 -4 1 0 -4 -3 -1 -4 -2 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.52 0.07 69 A 6 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.51 0.07 70 W -3 -4 -5 -6 -3 -3 -4 -3 -3 -4 -2 -4 -2 1 -5 -4 -3 14 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.50 0.07 K Lambda Standard Ungapped 0.1316 0.3161 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1522 0.3157 PSI Gapped 0.0466 0.2670