Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 D -2 -2 1 6 -4 0 2 -1 -1 -3 -4 -1 -3 -4 -2 0 -1 -5 -3 -3 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.00 2 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.47 0.00 3 T 0 -2 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -1 1 6 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.65 0.11 4 V 2 -3 -3 -3 -1 -2 -2 -2 -3 2 0 -2 0 -2 -2 -1 0 -3 -2 4 29 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 71 0.35 0.11 5 S 0 -2 -1 -1 -2 -2 -2 4 -2 -4 -4 -1 -3 -4 3 3 0 -4 -3 -3 0 0 0 0 0 0 0 40 0 0 0 0 0 0 17 43 0 0 0 0 0.67 0.20 6 E 0 -2 -1 0 -3 0 4 2 -2 -2 -3 -1 -2 -3 -2 2 -1 -4 -3 0 6 0 0 0 0 0 42 16 0 0 0 0 0 0 0 23 0 0 0 13 0.38 0.23 7 P -1 -3 -2 -2 -3 -2 -2 -3 -3 -4 -4 -2 -3 -4 8 0 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 92 8 0 0 0 0 2.08 0.25 8 A 5 -2 -3 -3 -1 -2 -2 -1 -3 -2 -2 -2 -2 -3 -2 0 -1 -4 -3 0 93 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0.76 0.31 9 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -5 8 -2 -2 -5 -4 -3 5 0 0 0 0 0 0 0 0 0 0 0 0 0 95 0 0 0 0 0 2.25 0.33 10 S 3 -2 -1 1 -3 1 4 -2 -2 -3 -3 -1 -2 -4 -2 1 -1 -4 -3 -2 38 0 0 6 0 5 41 0 0 0 0 0 0 0 0 11 0 0 0 0 0.56 0.33 11 C -1 -5 -4 -5 10 -4 -5 -4 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -3 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.08 0.34 12 V -1 -4 -4 -4 -2 -3 -4 -4 -4 2 0 -3 0 -2 -3 -3 -1 -4 -2 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.88 0.34 13 T 0 -2 -1 -1 -3 1 3 -3 5 -2 -3 -1 -2 -3 -2 -1 3 -4 -2 0 9 0 0 0 0 4 31 0 20 0 0 0 0 0 0 0 25 0 0 11 0.44 0.34 14 L -2 -3 -3 -4 -3 -2 -3 -4 3 1 1 -3 3 3 -4 0 -2 -1 5 0 0 0 0 0 0 0 0 0 9 6 10 0 15 12 0 8 0 0 32 7 0.50 0.34 15 Y 0 -2 1 -2 -3 -2 -2 0 2 -2 -2 -2 -2 4 -3 2 0 -1 4 -2 7 0 6 0 0 0 0 7 6 0 0 0 0 20 0 24 6 0 24 0 0.41 0.34 16 Q 3 -1 -1 -1 -3 5 2 -2 -1 -3 -3 0 -2 -4 -2 1 -1 -3 -3 -2 35 0 0 0 0 44 15 0 0 0 0 0 0 0 0 6 0 0 0 0 0.61 0.34 17 S 0 -2 0 3 -2 -1 -1 0 -2 -3 -4 -1 -3 -4 -2 4 1 -4 -3 -3 0 0 0 25 0 0 0 6 0 0 0 0 0 0 0 61 7 0 0 0 0.65 0.34 18 W -4 -4 -5 -5 -3 -3 -4 -4 -3 -4 -3 -4 -2 0 -5 -4 -3 12 1 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 3.08 0.34 19 R -2 7 -1 -3 -5 0 -1 -3 -1 -4 -3 1 -2 -4 -3 -2 -2 -4 -3 -4 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.32 0.34 20 Y -3 -3 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 2 -4 -3 -3 1 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.57 0.34 21 S 0 -2 -1 -2 -2 -2 -2 -2 -3 -2 -2 -2 -2 -3 -2 2 6 -4 -3 -1 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 83 0 0 0 0.83 0.34 22 Q -3 -2 1 4 -4 1 -1 -3 2 -3 -2 -2 -2 5 -3 -2 0 -2 0 -3 0 0 9 34 0 10 0 0 7 0 0 0 0 34 0 0 5 0 0 0 0.56 0.34 23 A 2 -3 -4 -4 -2 -3 -3 -3 -4 1 0 -3 -1 -2 -3 -2 -1 -4 -2 5 25 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 75 0.64 0.34 24 D 1 0 -1 1 -2 -1 -1 -2 4 -2 -2 -2 -2 0 -2 0 4 -3 -1 0 15 5 0 10 0 0 0 0 15 0 0 0 0 4 0 0 42 0 0 9 0.37 0.34 25 N -3 -1 7 0 -4 -1 -1 -1 0 -4 -5 -1 -3 -4 -3 0 -1 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.33 0.34 26 G 0 -3 4 2 -3 -2 -2 5 -2 -4 -4 -2 -3 -4 -3 -1 0 -4 -4 -3 7 0 24 12 0 0 0 49 0 0 0 0 0 0 0 0 7 0 0 0 0.73 0.34 27 C -1 -5 -4 -5 10 -4 -5 -4 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -3 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.08 0.34 28 A 3 -2 -1 1 -2 -1 1 1 -2 -3 -2 -1 -2 -3 1 3 -1 -4 -3 -2 37 0 0 7 0 0 9 9 0 0 4 0 0 0 6 28 0 0 0 0 0.39 0.34 29 E -2 -2 -1 4 -3 1 2 0 -2 1 -3 -1 -2 -3 -2 1 1 -4 -3 -1 0 0 0 33 0 5 16 9 0 12 0 0 0 0 0 14 7 0 0 4 0.39 0.34 30 T 3 -2 -1 0 -2 -2 -2 0 -3 -2 -2 -2 -2 -3 -2 0 5 -4 -3 -1 35 0 0 6 0 0 0 6 0 0 0 0 0 0 0 0 53 0 0 0 0.56 0.34 31 V -2 -3 -3 -4 -2 1 -2 -4 2 1 -1 -2 -1 0 -3 -2 -1 -1 5 4 0 0 0 0 0 9 0 0 6 0 0 0 0 0 0 0 0 0 27 58 0.57 0.34 32 T 1 1 -1 0 -2 -1 -1 0 3 -3 -3 -1 -2 -3 -2 3 3 -4 -2 -2 14 7 0 6 0 0 0 5 9 0 0 0 0 0 0 31 28 0 0 0 0.40 0.34 33 V -1 -3 -4 -4 -2 -3 -4 -4 -4 2 3 -3 0 -1 -4 -3 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 0 29 0 0 0 0 0 0 0 0 71 0.70 0.34 34 K 0 -1 -1 -2 -2 -1 -2 -3 -2 -1 -2 2 2 -3 -2 1 5 -3 -3 0 4 0 0 0 0 0 0 0 0 0 0 16 9 0 0 5 60 0 0 5 0.54 0.34 35 V -1 -4 -4 -4 -2 -3 -4 -4 -4 2 1 -3 0 -2 -3 -3 -1 -4 -2 6 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 0 0 0 0 91 0.80 0.34 36 V 2 1 -2 -1 -3 1 4 -3 -2 -1 -2 -1 -2 -3 -2 -1 -1 -4 -3 3 19 9 0 0 0 5 36 0 0 0 0 0 0 0 0 0 0 0 0 31 0.38 0.34 37 Y -3 -3 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 2 -4 -3 -3 1 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.57 0.34 38 E -1 2 -1 -1 -3 2 2 0 -2 -2 0 -1 -2 -3 -2 1 4 -3 -3 -2 0 12 0 0 0 15 15 6 0 0 9 0 0 0 0 7 36 0 0 0 0.36 0.34 39 D -3 -2 4 6 -4 -1 0 -2 1 -4 -5 -1 -4 -4 -3 -1 -2 -5 -3 -4 0 0 28 67 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 1.09 0.32 40 D -1 -3 -1 2 -4 -3 -2 6 -3 -5 -5 -2 -4 -4 -3 -1 -2 -4 -4 -4 0 0 0 12 0 0 0 88 0 0 0 0 0 0 0 0 0 0 0 0 1.28 0.32 41 T 2 1 -1 -2 -2 4 -1 0 -2 -3 -3 -1 -2 -4 -2 0 3 -3 -3 -2 23 7 0 0 0 33 0 7 0 0 0 0 0 0 0 0 31 0 0 0 0.46 0.32 42 E -2 -2 1 2 -4 -1 3 0 -2 -4 -3 -1 -3 -2 -3 -1 2 7 -1 -3 0 0 6 16 0 0 31 7 0 0 0 0 0 0 0 0 16 23 0 0 0.72 0.32 43 G 3 -3 -3 -3 -2 -3 -3 3 -3 0 -2 -2 -1 -3 -3 -1 -1 -4 -3 3 33 0 0 0 0 0 0 28 0 0 0 0 0 0 0 0 0 0 0 40 0.48 0.32 44 L -2 -3 -3 -3 -4 -2 -2 -3 -3 -2 1 -2 -2 -3 8 -2 -2 -4 -4 -2 0 0 0 0 0 0 0 0 0 0 17 0 0 0 83 0 0 0 0 0 1.82 0.32 45 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -4 0 -2 -3 -3 -2 0 0 0 0 94 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 1.87 0.32 46 Y 0 6 -2 -3 -3 0 -1 -3 -1 -3 -3 1 -2 -2 -3 1 -2 -2 2 -1 6 65 0 0 0 0 0 0 0 0 0 0 0 0 0 10 0 0 12 7 0.70 0.32 47 A 1 -3 -2 -2 -2 -2 0 -3 -3 1 -1 -2 -1 -2 -3 0 3 -4 -2 3 12 0 0 0 0 0 7 0 0 5 0 0 0 0 0 6 30 0 0 40 0.37 0.32 48 V 2 -3 -4 -4 -2 -3 -3 -3 -4 3 2 -3 0 -1 -3 -2 -1 -3 -2 3 26 0 0 0 0 0 0 0 0 23 26 0 0 0 0 0 0 0 0 26 0.43 0.32 49 A 2 -1 1 -1 -3 3 1 0 2 -3 -3 -1 -2 -4 3 1 -1 -4 -2 -3 23 0 6 0 0 22 8 5 7 0 0 0 0 0 17 12 0 0 0 0 0.44 0.32 50 P -2 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -4 -5 8 -2 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.41 0.32 51 G -1 -3 -1 -2 -4 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -3 -4 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.50 0.32 52 Q 3 -2 -1 3 -3 2 1 1 -2 -3 -3 -1 -2 -3 -2 -1 -2 4 -2 -2 40 0 0 19 0 16 10 8 0 0 0 0 0 0 0 0 0 7 0 0 0.45 0.32 53 I 0 2 -2 -3 -2 -2 -2 -3 -2 3 -1 -1 2 -1 -3 0 2 6 1 1 7 16 0 0 0 0 0 0 0 24 0 0 6 0 0 9 14 12 5 6 0.32 0.32 54 T 3 -3 -2 -3 -2 -2 -2 -2 -3 1 0 -2 -1 -2 -2 1 3 -4 -2 2 33 0 0 0 0 0 0 0 0 6 6 0 0 0 0 10 24 0 0 20 0.35 0.32 55 T -1 -2 -1 -2 -2 -2 -2 -3 -3 -2 -2 -2 -2 0 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 6 87 0 0 0 0.82 0.32 56 V -1 -4 -4 -4 -3 -4 -4 -4 -3 0 0 -4 -1 7 -4 -3 -2 -1 1 3 7 0 0 0 0 0 0 0 0 0 0 0 0 65 0 0 0 0 0 28 0.83 0.32 57 G -1 -3 -1 -2 -4 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -3 -4 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.50 0.32 58 D -3 -2 -2 2 -4 -1 2 -4 4 -3 -3 -2 -2 1 -3 -2 -2 0 7 -3 0 0 0 12 0 0 16 0 13 0 0 0 0 0 0 0 0 0 59 0 0.88 0.32 59 G -1 -3 -1 -2 -4 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -3 -4 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.50 0.32 60 Y -1 -2 -2 -2 -2 -2 -2 -3 -1 -2 -2 -2 -2 -1 1 0 5 -1 5 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 65 0 28 0 0.72 0.32 61 I -2 2 3 2 -3 2 -1 0 -2 1 1 -1 -1 -3 -3 -1 0 -4 -3 -1 0 12 16 16 0 14 0 7 0 12 16 0 0 0 0 0 6 0 0 0 0.22 0.32 62 G -1 -3 -1 -2 -3 -3 -3 6 -3 -5 -5 -2 -4 -4 -3 0 -2 -4 -4 -4 0 0 0 0 0 0 0 93 0 0 0 0 0 0 0 7 0 0 0 0 1.36 0.32 63 S -2 2 6 0 -3 -1 0 -2 -1 -4 -4 -1 -3 -4 -3 1 -1 -4 -3 -4 0 11 70 0 0 0 6 0 0 0 0 0 0 0 0 13 0 0 0 0 0.86 0.31 64 H -2 -2 -1 -3 -3 -1 -2 -3 7 -3 -2 -2 -2 1 -3 -2 -2 0 6 -3 0 0 0 0 0 0 0 0 46 0 0 0 0 0 0 0 0 0 54 0 1.01 0.19 65 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -3 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.16 0.08 66 H -2 0 0 -1 -3 0 0 -2 8 -4 -3 -1 -2 -2 -3 -1 -2 -3 2 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 1.06 0.08 67 A 3 -2 -2 -2 -2 -1 -1 -1 -2 -2 -3 -1 -2 -3 6 0 -1 -4 -3 -2 41 0 0 0 0 0 0 0 0 0 0 0 0 0 59 0 0 0 0 0 1.13 0.08 68 R -2 4 1 5 -4 0 1 -2 -1 -3 -3 1 -3 -4 -2 -1 -1 -4 -3 -3 0 41 0 59 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.68 0.08 69 Y -2 -1 0 -2 -3 0 -1 -3 7 -3 -2 -1 -2 1 -3 -1 -2 0 5 -3 0 0 0 0 0 0 0 0 59 0 0 0 0 0 0 0 0 0 41 0 0.81 0.08 70 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.57 0.08 71 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.51 0.08 72 R 1 4 -2 -3 -2 -1 -1 -2 -2 0 1 0 0 -2 -2 -1 -1 -3 -2 1 19 41 0 0 0 0 0 0 0 0 20 0 0 0 0 0 0 0 0 20 0.23 0.08 73 C -1 -4 -3 -4 9 -3 -4 -3 -3 -2 -2 -3 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.42 0.08 74 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.45 0.00 K Lambda Standard Ungapped 0.1325 0.3166 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1611 0.3182 PSI Gapped 0.0493 0.2670