Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 G 0 -3 -1 -2 -3 -2 -3 6 -3 -5 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.32 0.18 2 E -1 4 -1 -1 -4 1 3 2 -1 -4 -3 3 -2 -4 -2 -1 -2 -3 -3 -3 0 38 0 0 0 0 25 18 0 0 0 20 0 0 0 0 0 0 0 0 0.54 0.18 3 Q -2 0 0 -1 -3 5 0 -3 6 -3 -3 0 -1 -3 -2 -1 2 -3 0 -3 0 0 0 0 0 42 0 0 38 0 0 0 0 0 0 0 20 0 0 0 0.65 0.18 4 K -1 1 -1 0 -4 1 5 -3 -1 -4 -3 4 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 56 0 0 0 0 44 0 0 0 0 0 0 0 0 0.71 0.18 5 E -1 -1 -1 1 -4 1 5 2 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 80 20 0 0 0 0 0 0 0 0 0 0 0 0 0.78 0.18 6 E -2 0 -1 0 -4 1 4 -3 -1 1 -2 3 -1 -3 -2 -1 -1 -4 -2 -1 0 0 0 0 0 0 46 0 0 20 0 34 0 0 0 0 0 0 0 0 0.45 0.18 7 L -2 -2 -2 3 -3 -1 2 -3 -2 0 3 -2 0 -1 -3 -2 -2 -3 -2 -1 0 0 0 20 0 0 22 0 0 0 59 0 0 0 0 0 0 0 0 0 0.37 0.18 8 P -2 2 -2 -2 -3 -1 -1 -3 -2 -1 -2 3 -2 3 4 -2 -2 -3 -1 1 0 14 0 0 0 0 0 0 0 0 0 33 0 19 20 0 0 0 0 15 0.41 0.23 9 E -2 0 3 0 -4 3 4 -2 -1 -3 -3 2 -2 -4 -2 0 2 -4 -3 -3 0 0 19 0 0 18 33 0 0 0 0 15 0 0 0 0 15 0 0 0 0.51 0.23 10 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 0 3 -4 -3 -1 -3 -1 3 0 0 0 0 0 0 0 0 0 52 0 0 0 15 0 0 0 0 0 33 0.57 0.23 11 A 1 4 -2 -2 -3 0 2 -2 -2 -1 -2 2 -2 -3 -2 -1 -1 -4 -2 1 20 29 0 0 0 0 15 0 0 0 0 19 0 0 0 0 0 0 0 18 0.33 0.23 12 G 0 -3 -1 -2 -3 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.38 0.23 13 V -2 -3 -3 -4 -2 -3 -3 -4 -2 3 0 -3 0 3 -3 -2 1 -1 3 3 0 0 0 0 0 0 0 0 0 28 0 0 0 14 0 0 15 0 15 29 0.39 0.23 14 L -2 -3 -4 -5 -2 -3 -4 -4 -3 1 4 -3 1 4 -4 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 70 0 0 30 0 0 0 0 0 0 0.67 0.23 15 S 2 -2 -1 -2 4 -2 -2 -2 -2 -1 -2 -2 -1 -3 -2 3 2 -3 -2 1 15 0 0 0 14 0 0 0 0 0 0 0 0 0 0 37 15 0 0 19 0.37 0.23 16 D -1 -2 0 4 -4 -1 2 4 -2 -4 -4 -1 -4 -4 -2 -1 -2 -4 -4 -4 0 0 0 37 0 0 19 44 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.23 17 E -2 -2 3 5 -4 0 4 -2 -1 -4 -4 -1 -3 -4 -2 -1 -1 -5 -3 -4 0 0 19 48 0 0 33 0 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.23 18 Y -2 6 -1 -3 -4 0 -1 -3 0 -3 -3 1 -2 -1 -3 -2 -2 -2 4 -3 0 80 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 20 0 0.90 0.23 19 V -1 -3 -4 -4 -2 -3 -3 -4 -4 3 0 -3 0 -1 -3 -3 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 19 0 0 0 0 0 0 0 0 0 81 0.66 0.23 20 I -2 -4 -4 -4 -2 -3 -4 -4 -4 4 2 -3 0 3 -4 -3 -2 -2 0 3 0 0 0 0 0 0 0 0 0 48 15 0 0 19 0 0 0 0 0 18 0.53 0.23 21 T -1 -2 -1 -2 -2 -2 -2 -3 -3 0 -1 -2 -1 -2 -2 0 5 -3 -2 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 69 0 0 31 0.57 0.23 22 K -2 -1 0 5 -4 0 2 -2 -2 -3 -3 2 -3 3 -2 -1 -2 -3 -1 -3 0 0 0 49 0 0 14 0 0 0 0 20 0 18 0 0 0 0 0 0 0.59 0.23 23 Q -2 2 0 5 -4 3 0 2 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -4 0 15 0 46 0 20 0 19 0 0 0 0 0 0 0 0 0 0 0 0 0.63 0.23 24 T -2 -1 -2 -3 -3 -1 -2 -3 -2 -1 1 3 -1 3 -3 -1 2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 15 33 0 19 0 0 20 0 0 14 0.25 0.23 25 E -1 -1 3 1 -3 1 5 -2 0 -3 -3 0 -2 -3 -1 0 -1 -3 -2 -2 1 1 19 1 0 1 64 1 0 1 2 1 0 1 1 1 1 0 1 1 0.61 0.18 26 I -2 -4 -3 -4 -2 -3 -4 2 -4 5 2 -3 0 -1 -4 -3 -2 -3 -2 1 0 0 0 0 0 0 0 18 0 63 19 0 0 0 0 0 0 0 0 0 0.48 0.23 27 F -3 -3 -3 -4 -3 -3 -3 -4 4 2 0 -3 -1 6 -4 -3 -3 -1 2 -1 0 0 0 0 0 0 0 0 19 18 0 0 0 63 0 0 0 0 0 0 0.75 0.23 28 S 0 4 0 -1 -3 0 2 -2 -1 -4 -3 0 -2 -3 -2 4 0 -4 -3 -3 0 33 0 0 0 0 15 0 0 0 0 0 0 0 0 52 0 0 0 0 0.54 0.23 29 R -2 5 -1 -1 -4 0 2 -3 -1 -3 -3 2 -2 -4 -2 -1 2 -4 -3 -3 0 52 0 0 0 0 15 0 0 0 0 14 0 0 0 0 19 0 0 0 0.62 0.23 30 Y -3 -2 -3 -4 -3 -2 -3 -4 4 -1 1 -3 -1 4 -4 -3 -2 1 6 -2 0 0 0 0 0 0 0 0 15 0 18 0 0 15 0 0 0 0 52 0 0.78 0.23 31 F -3 -3 -4 -4 -3 -4 -4 -4 -1 -1 0 -4 -1 7 -4 -3 -3 1 5 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 82 0 0 0 0 18 0 1.12 0.23 32 Y -3 -3 -3 -4 -3 -2 -3 -4 1 -2 -1 -3 -2 4 -4 -3 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 15 0 0 0 0 85 0 1.23 0.23 33 G 0 -3 -1 -2 -3 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.38 0.23 34 S 0 -1 0 -1 -2 0 2 -1 -2 -3 -3 -1 -2 -3 -2 5 2 -4 -3 -2 0 0 0 0 0 0 18 0 0 0 0 0 0 0 0 69 14 0 0 0 0.57 0.23 35 E -2 -1 -2 0 -3 0 4 -3 -1 -1 1 2 4 -2 -2 -1 -2 -3 -2 -2 0 0 0 0 0 0 48 0 0 0 15 18 19 0 0 0 0 0 0 0 0.44 0.23 36 K -1 1 3 -1 -4 0 0 2 -1 -4 -4 5 -2 -4 -2 -1 -1 -4 -3 -3 0 0 18 0 0 0 0 15 0 0 0 67 0 0 0 0 0 0 0 0 0.61 0.23 37 G -1 -3 0 4 -3 -2 -1 4 -2 -4 -4 -2 -3 -3 -3 1 -2 6 -2 -4 0 0 0 33 0 0 0 39 0 0 0 0 0 0 0 14 0 15 0 0 0.73 0.23 38 D -1 -3 0 5 -4 -2 0 4 -2 -4 -5 -2 -4 -4 -3 -1 -2 -4 -4 -4 0 0 0 56 0 0 0 44 0 0 0 0 0 0 0 0 0 0 0 0 0.92 0.23 39 L -2 -3 -3 -4 -2 -2 -3 -4 -2 1 2 -3 5 3 -4 -3 -2 -1 3 2 0 0 0 0 0 0 0 0 0 0 20 0 34 18 0 0 0 0 14 15 0.48 0.23 40 V -1 -3 -3 -4 -2 -3 -3 -4 -4 1 2 -3 0 -1 -3 -2 2 -3 -2 4 0 0 0 0 0 0 0 0 0 0 18 0 0 0 0 0 19 0 0 63 0.47 0.23 41 T 2 -2 -1 -2 -2 -2 -2 -2 -3 2 -1 -2 -1 -2 -2 2 3 -3 -2 0 29 0 0 0 0 0 0 0 0 19 0 0 0 0 0 18 35 0 0 0 0.36 0.23 42 L -2 -3 -4 -5 -2 -3 -4 -5 -4 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.79 0.23 43 S 1 -1 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 5 1 -4 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.77 0.23 44 L -2 -3 -4 -4 -2 -3 -4 -5 -4 4 4 -3 1 0 -4 -3 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 34 66 0 0 0 0 0 0 0 0 0 0.64 0.23 45 I -2 -3 -4 -4 -2 -3 -4 -5 -4 5 3 -3 1 -1 -4 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 52 34 0 0 0 0 0 0 0 0 14 0.59 0.23 46 E -2 -1 -1 1 -5 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 1.07 0.23 47 S 3 -2 -1 -2 -2 -1 -1 2 -2 -3 -3 -1 -2 -3 -2 4 0 -3 -3 -2 33 0 0 0 0 0 0 19 0 0 0 0 0 0 0 48 0 0 0 0 0.52 0.23 48 L 1 -3 -4 -4 -2 -3 -3 -3 -3 1 5 -3 1 -1 -3 -2 -2 -3 -2 0 19 0 0 0 0 0 0 0 0 0 81 0 0 0 0 0 0 0 0 0 0.56 0.23 49 Y -3 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 3 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.36 0.23 50 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 4 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 85 0 15 0 0 0 0 0 0 0 0.71 0.23 51 L -1 -3 -3 -4 -2 -3 -3 -4 -4 3 3 -3 1 -1 -3 -2 2 -3 -2 3 0 0 0 0 0 0 0 0 0 15 33 0 0 0 0 0 18 0 0 34 0.42 0.23 52 D -1 -1 0 4 -4 0 4 -2 -1 -4 -4 0 -3 -4 -2 2 -1 -4 -3 -3 0 0 0 35 0 0 48 0 0 0 0 0 0 0 0 18 0 0 0 0 0.74 0.23 53 L 1 0 -2 -2 -3 0 2 -3 -2 -1 2 3 0 -2 -2 -1 -1 -3 -2 -1 15 0 0 0 0 0 15 0 0 0 37 33 0 0 0 0 0 0 0 0 0.27 0.23 54 G 0 -3 -1 -2 -3 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.38 0.23 55 K -2 -2 -3 -4 5 -2 -3 -3 -3 1 -1 2 -1 4 -4 -2 -2 7 1 -1 0 0 0 0 19 0 0 0 0 15 0 20 0 29 0 0 0 18 0 0 0.60 0.23 56 L -2 -3 -4 -5 -2 -3 -4 -5 -4 1 5 -3 1 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.80 0.24 57 N -1 -2 2 -1 -3 0 3 -3 -2 2 -1 -1 -1 -3 -2 -1 2 -4 -2 2 0 0 15 0 0 0 35 0 0 16 0 0 0 0 0 0 16 0 0 19 0.31 0.24 58 L -1 -2 -3 -3 -1 -2 -2 -3 -3 1 2 -2 4 -1 -2 -2 -1 -2 -2 4 1 1 1 1 0 1 1 1 0 1 16 1 16 1 1 1 1 0 1 55 0.38 0.20 59 L -2 -1 -2 2 -3 -1 -1 -3 -1 -1 2 2 -1 0 -3 -2 -2 -1 5 -1 0 0 0 16 0 0 0 0 0 0 30 19 0 0 0 0 0 0 35 0 0.39 0.24 60 N 1 -1 4 3 -3 0 2 -2 -1 -4 -4 2 -3 -4 -2 0 -1 -4 -3 -3 16 0 30 19 0 0 16 0 0 0 0 19 0 0 0 0 0 0 0 0 0.48 0.24 61 A 3 2 -2 -2 -2 -1 1 -2 -2 -1 2 -1 0 -2 -2 -1 -1 -3 -2 -1 34 16 0 0 0 0 16 0 0 0 35 0 0 0 0 0 0 0 0 0 0.26 0.24 62 D 0 -2 0 2 -3 -1 2 4 -2 -4 -4 -1 -3 -4 -2 3 -1 -4 -3 -3 0 0 0 15 0 0 16 35 0 0 0 0 0 0 0 35 0 0 0 0 0.54 0.24 63 R 1 4 -2 -3 -3 -1 -2 -3 -2 -1 1 0 -1 3 -3 -1 -2 -2 0 -1 19 38 0 0 0 0 0 0 0 0 21 0 0 22 0 0 0 0 0 0 0.33 0.20 64 E -2 -1 3 1 -4 1 6 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 21 0 0 0 79 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.20 65 E -2 -1 -1 1 -5 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 1.04 0.20 66 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 4 -3 1 4 -4 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 79 0 0 21 0 0 0 0 0 0 0.65 0.20 67 V -2 4 -2 -3 -3 -1 -2 -3 -1 -1 -2 0 -1 0 -3 -2 -2 7 4 1 0 40 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 20 22 18 0.66 0.20 68 K 2 0 -1 -1 -3 0 3 -2 -1 -3 -3 4 -2 -4 -2 2 -1 -4 -3 -2 21 0 0 0 0 0 22 0 0 0 0 38 0 0 0 19 0 0 0 0 0.42 0.20 69 R 3 3 -2 -2 -2 -1 -1 -2 -1 -3 -2 2 -2 -1 -2 0 -1 -1 4 -2 39 18 0 0 0 0 0 0 0 0 0 21 0 0 0 0 0 0 22 0 0.37 0.20 70 A 4 -2 -2 -2 -1 -2 -2 -1 -2 -1 -2 -2 -1 -3 -2 2 0 -3 -2 2 61 0 0 0 0 0 0 0 0 0 0 0 0 0 0 20 0 0 0 19 0.43 0.20 71 R -2 4 -1 3 -4 0 2 -3 -1 -2 1 0 -1 -3 -3 -1 -2 -4 -3 -2 0 40 0 19 0 0 21 0 0 0 20 0 0 0 0 0 0 0 0 0 0.44 0.20 72 E 2 -1 3 0 -3 0 4 -1 -1 -3 -3 0 -2 -4 -2 2 -1 -4 -3 -2 21 0 22 0 0 0 37 0 0 0 0 0 0 0 0 20 0 0 0 0 0.46 0.20 73 V -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 76 0 0 0 0 0 0 0 0 0 24 0.60 0.16 74 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.16 75 R -1 5 0 -2 -3 1 0 -2 0 -3 -2 2 -1 -3 -2 -1 -1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 K Lambda Standard Ungapped 0.1367 0.3133 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1684 0.3181 PSI Gapped 0.0515 0.2670