Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 H -2 0 1 -1 -3 0 0 -2 8 -3 -3 -1 -2 -1 -2 -1 -2 -2 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 2 M -1 -2 -3 -3 -1 -1 -2 -3 -2 2 2 -2 5 0 -3 -2 -1 -2 -1 2 0 0 0 0 0 0 0 0 0 0 0 0 67 0 0 0 0 0 0 33 0.28 0.01 3 P -1 -2 -2 -2 -3 -1 -1 -2 -2 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.05 0.01 4 V 0 -3 -3 -4 -1 -2 -3 -4 -4 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.36 0.01 5 P -1 -2 -2 -2 -3 -1 -1 -2 -2 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.05 0.01 6 S 1 -1 0 -1 -1 0 0 -1 -1 -2 -2 0 -1 -3 -1 4 3 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 74 26 0 0 0 0.41 0.01 7 F -3 -3 -3 -4 -3 -4 -4 -4 -1 0 0 -3 0 7 -4 -3 -2 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.76 0.01 8 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -2 0 -2 -3 -3 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.06 0.01 9 E -1 0 0 2 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.75 0.01 10 A 4 -2 -2 -2 0 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.42 0.01 11 M -1 -2 -2 -3 -2 -1 -2 -3 -2 1 2 -2 6 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.36 0.01 12 A 4 -2 -2 -2 0 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.42 0.01 13 Y -2 -2 -2 -3 -3 -2 -2 -3 2 -2 -1 -2 -1 3 -3 -2 -2 2 7 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0.88 0.01 14 F -3 -3 -3 -4 -3 -4 -4 -4 -1 0 0 -3 0 7 -4 -3 -2 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.76 0.01 15 A 4 -2 -2 -2 0 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.42 0.01 16 M -1 -2 -2 -3 -2 -1 -2 -3 -2 1 2 -2 6 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.36 0.01 17 V 0 -3 -3 -4 -1 -2 -3 -4 -4 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.36 0.01 18 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.53 0.01 19 R -2 6 -1 -2 -4 1 0 -3 0 -3 -2 2 -2 -3 -2 -1 -1 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.01 20 Y -2 -2 -2 -3 -3 -2 -2 -3 2 -2 -1 -2 -1 3 -3 -2 -2 2 7 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0.88 0.01 21 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.47 0.01 22 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 2 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.48 0.01 23 S 1 -1 1 0 -1 0 0 0 -1 -3 -3 0 -2 -3 -1 4 2 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.47 0.01 24 F -3 -3 -3 -4 -3 -4 -4 -4 -1 0 0 -3 0 7 -4 -3 -2 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.76 0.01 25 P -1 -2 -2 -2 -3 -1 -1 -2 -2 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.05 0.01 26 I -2 -3 -4 -4 -1 -3 -4 -4 -4 5 2 -3 1 0 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.41 0.01 27 D -2 -2 1 6 -4 0 2 -2 -1 -4 -4 -1 -3 -4 -2 0 -1 -5 -3 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.95 0.01 28 D -2 -1 1 6 -4 0 3 -2 -1 -4 -4 0 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 74 0 0 26 0 0 0 0 0 0 0 0 0 0 0 0 0 0.78 0.01 29 R -2 6 -1 -2 -4 1 0 -3 0 -3 -2 2 -2 -3 -2 -1 -1 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.01 30 V 0 -3 -3 -4 -1 -2 -3 -4 -4 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.36 0.01 31 Q -1 1 0 0 -3 6 2 -2 0 -3 -2 1 -1 -4 -1 0 -1 -2 -2 -2 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.67 0.01 32 S 1 -1 1 0 -1 0 0 0 -1 -3 -3 0 -2 -3 -1 4 2 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.47 0.01 33 H -2 0 1 -1 -3 0 0 -2 8 -4 -3 -1 -2 -1 -2 -1 -2 -3 2 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0.88 0.01 34 I -2 -3 -4 -4 -1 -3 -4 -4 -4 5 2 -3 1 0 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.41 0.01 35 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.47 0.01 36 H -2 0 1 -1 -3 0 0 -2 8 -4 -3 -1 -2 -1 -2 -1 -2 -3 2 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0.88 0.01 37 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.47 0.01 38 E -1 0 0 2 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.75 0.01 39 H -2 0 1 -1 -3 0 0 -2 8 -4 -3 -1 -2 -1 -2 -1 -2 -3 2 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0.88 0.01 40 D -2 -2 1 6 -4 0 2 -2 -1 -4 -4 -1 -3 -4 -2 0 -1 -5 -3 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.95 0.01 41 L -2 -2 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.47 0.01 42 V 0 -3 -3 -4 -1 -2 -3 -4 -4 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.36 0.01 43 H -2 0 1 -1 -3 0 0 -2 8 -4 -3 -1 -2 -1 -2 -1 -2 -3 2 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0.88 0.01 44 V 0 -3 -3 -4 -1 -2 -3 -4 -4 3 1 -3 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.36 0.01 45 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 2 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.48 0.01 46 R -1 6 0 -2 -4 1 0 -2 0 -3 -3 3 -2 -3 -2 -1 -1 -3 -2 -3 0 74 0 0 0 0 0 0 0 0 0 26 0 0 0 0 0 0 0 0 0.65 0.01 47 K -1 4 0 -1 -4 1 1 -2 -1 -3 -3 5 -2 -3 -1 0 -1 -3 -2 -3 0 26 0 0 0 0 0 0 0 0 0 74 0 0 0 0 0 0 0 0 0.53 0.01 48 N -1 -1 6 1 -2 0 0 0 0 -3 -4 0 -2 -3 -2 2 0 -4 -2 -3 0 0 74 0 0 0 0 0 0 0 0 0 0 0 0 26 0 0 0 0 0.56 0.01 K Lambda Standard Ungapped 0.1383 0.3279 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1496 0.3186 PSI Gapped 0.0458 0.2670