Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.11 2 T 0 -2 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -1 1 6 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.65 0.11 3 C -1 -4 -3 -4 10 -3 -4 -3 -3 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.52 0.11 4 G 0 -3 -1 -2 -3 -2 -3 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.21 0.11 5 S 3 -2 -1 -1 -1 -1 -1 2 -2 -2 -3 -1 -2 -3 -1 3 0 -3 -2 -1 42 0 0 0 0 0 0 12 0 0 0 0 0 0 0 46 0 0 0 0 0.42 0.11 6 G 0 -3 -1 -2 -3 -2 -3 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.21 0.11 7 Y -2 -2 -3 -4 -3 -2 -2 -4 1 -2 -1 -2 -1 3 -3 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.11 0.11 8 N -1 -2 2 2 -3 -2 -1 5 -2 -4 -4 -2 -3 -4 -2 0 -2 -3 -3 -4 0 0 13 13 0 0 0 74 0 0 0 0 0 0 0 0 0 0 0 0 0.86 0.11 9 V 0 -3 -1 -2 -2 -2 -2 5 -3 -1 -2 -2 -2 -3 2 -1 -1 -3 -3 1 0 0 0 0 0 0 0 63 0 0 0 0 0 0 13 0 0 0 0 24 0.63 0.11 10 D 0 -2 1 6 -3 -1 1 -1 -2 -3 -4 -1 -3 -4 -2 0 -1 -4 -3 -3 13 0 0 87 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.11 11 Q -1 1 0 -1 -3 6 2 -2 0 -3 -3 1 -1 -4 -2 0 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.11 12 R -2 6 -1 -2 -4 1 0 -3 -1 -3 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.95 0.11 13 R -2 6 -1 -2 -4 1 0 -3 -1 -3 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.95 0.11 14 T 0 -2 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -1 1 6 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.65 0.11 15 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -3 -2 0 0 -4 -3 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.11 16 S 0 2 0 -1 -2 0 0 -1 -1 -3 -3 0 -2 -3 -1 4 1 -3 -2 -2 0 21 0 0 0 0 0 0 0 0 0 0 0 0 0 79 0 0 0 0 0.49 0.11 17 G -1 -3 -2 -2 -3 -2 -2 1 -3 -2 1 -2 -1 -3 7 -1 -2 -3 -3 -2 0 0 0 0 0 0 0 13 0 0 21 0 0 0 67 0 0 0 0 0 1.20 0.11 18 C -1 -4 -3 -4 9 -3 -4 -3 -4 0 -1 -3 -1 -2 -3 -1 -1 -3 -3 1 0 0 0 0 79 0 0 0 0 0 0 0 0 0 0 0 0 0 0 21 1.13 0.11 19 K -2 0 1 -1 -3 4 2 -2 0 -3 -3 2 -1 -2 -2 -1 -1 7 -1 -3 0 0 10 0 0 45 12 0 0 0 0 13 0 0 0 0 0 21 0 0 0.64 0.11 20 A 3 -2 -1 -2 -1 -1 -1 -1 -1 -2 -2 -1 -1 -1 -2 2 0 -1 3 -1 57 0 0 0 0 0 0 0 0 0 0 0 0 0 0 22 0 0 21 0 0.37 0.11 21 G 1 -2 0 -1 -1 -1 -1 1 -2 -1 -2 -1 -1 -2 -2 4 1 -3 -2 1 0 0 0 0 0 0 0 13 0 0 0 0 0 0 0 67 0 0 0 21 0.33 0.11 22 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -3 -2 0 0 -4 -3 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.11 23 G 0 -3 -1 -2 -3 -2 -2 6 -3 -4 -4 -2 -3 -4 3 -1 -2 -3 -4 -3 0 0 0 0 0 0 0 79 0 0 0 0 0 0 21 0 0 0 0 0 1.04 0.11 24 D -1 -2 1 6 -3 -1 1 -1 -1 -3 -4 -1 -3 -4 -2 2 -1 -4 -3 -3 0 0 0 79 0 0 0 0 0 0 0 0 0 0 0 21 0 0 0 0 0.85 0.11 25 R -2 6 -1 -2 -4 3 0 -3 0 -3 -3 2 -2 -3 -2 -1 -1 -3 -2 -3 0 79 0 0 0 21 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.80 0.11 26 H -1 -1 0 -1 -3 0 0 -2 8 -4 -3 -1 -2 -2 -2 1 -1 -3 1 -3 0 0 0 0 0 0 0 0 79 0 0 0 0 0 0 21 0 0 0 0 0.86 0.11 27 F -3 -3 -3 -4 -3 -4 -4 -4 -2 0 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.98 0.11 28 C 1 -3 -3 -4 9 -3 -3 -2 -3 -2 -2 -3 -2 -3 -3 -1 -1 -3 -3 -1 21 0 0 0 79 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.13 0.11 29 G 0 -2 -1 -2 -3 -2 -2 6 4 -4 -4 -2 -3 -3 -3 -1 -2 -3 -2 -4 0 0 0 0 0 0 0 79 21 0 0 0 0 0 0 0 0 0 0 0 0.93 0.11 30 C -1 -4 -2 -3 9 -3 -4 2 -3 -2 -2 -3 -2 -3 -3 -1 -1 -3 -3 -2 0 0 0 0 79 0 0 21 0 0 0 0 0 0 0 0 0 0 0 0 1.16 0.11 31 D -2 -2 1 6 -4 -1 1 -2 -1 -3 -3 -1 -3 -1 -2 -1 -2 -2 3 -3 0 0 0 79 0 0 0 0 0 0 0 0 0 0 0 0 0 0 21 0 0.82 0.11 32 R -1 6 -1 -2 -3 1 0 -2 -1 -3 -3 2 -2 -3 -2 1 -1 -3 -2 -3 0 79 0 0 0 0 0 0 0 0 0 0 0 0 0 21 0 0 0 0 0.71 0.11 33 T -1 -2 -1 -2 -2 -1 -2 -2 -2 -1 -1 -1 -1 3 -2 1 5 -2 0 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 21 0 0 79 0 0 0 0.48 0.11 34 G 0 -1 -1 -2 -3 -1 -2 6 -2 -4 -4 2 -3 -4 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 0 79 0 0 0 21 0 0 0 0 0 0 0 0 0.88 0.11 35 V -1 -1 -3 -3 -2 -2 -2 -3 -3 3 0 1 0 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 25 0 21 0 0 0 0 0 0 0 54 0.30 0.11 36 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.52 0.11 37 E -1 0 -1 1 -4 3 5 -3 0 -4 -3 1 -2 -4 -2 0 -1 -3 -2 -3 0 0 0 0 0 12 88 0 0 0 0 0 0 0 0 0 0 0 0 0 0.83 0.11 38 C -1 -4 -3 -4 10 -3 -4 -3 -3 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.52 0.11 39 K -2 5 -1 -2 -3 1 0 -3 -1 0 -2 4 -1 -3 -2 -1 -1 -3 -2 -2 0 51 0 0 0 0 0 0 0 12 0 37 0 0 0 0 0 0 0 0 0.54 0.11 40 G -1 -2 4 -1 -3 -1 -2 5 -1 -4 -4 -1 -3 -4 -3 0 -1 -3 -3 -4 0 0 31 0 0 0 0 69 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.11 41 G 0 -3 -1 -2 -3 -2 -3 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.21 0.11 42 K -1 4 -1 -1 -3 1 0 -2 -1 -3 -3 4 -2 -3 -2 0 1 -3 -2 -2 0 31 0 0 0 0 0 0 0 0 0 57 0 0 0 0 12 0 0 0 0.56 0.11 43 W -3 -3 -4 -5 -3 -2 -3 -3 -3 -3 -2 -3 -2 1 -4 -3 -3 11 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.59 0.11 44 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -1 2 5 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12 88 0 0 0 0.59 0.11 45 E -1 0 -1 1 -4 2 6 -3 0 -4 -3 1 -2 -4 -2 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.11 46 V -1 -3 -4 -4 -2 -3 -3 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 74 0 0 0 0 0 0 0 0 0 26 0.52 0.11 47 Q -1 3 0 -1 -3 6 1 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 20 0 0 0 80 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.74 0.11 48 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.11 49 C -1 -4 -3 -4 10 -3 -4 -3 -3 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.52 0.11 50 G -1 2 0 -2 -3 0 -1 3 6 -4 -3 0 -2 -2 -3 -1 -2 -3 0 -3 0 20 0 0 0 0 0 34 46 0 0 0 0 0 0 0 0 0 0 0 0.62 0.11 51 S 3 -2 0 -1 -1 -1 -1 1 -2 -2 -2 -1 -2 -3 -1 3 2 -3 -2 -1 31 0 0 0 0 0 0 12 0 0 0 0 0 0 0 44 13 0 0 0 0.39 0.11 52 S 2 -2 2 -1 -2 -1 -1 3 -1 -3 -3 -1 -2 -3 -2 3 0 -3 -2 -2 22 0 13 0 0 0 0 22 0 0 0 0 0 0 0 43 0 0 0 0 0.41 0.11 53 S 0 -1 0 -1 -1 -1 -1 -1 -2 -2 -2 -1 -1 -3 -1 3 4 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 37 63 0 0 0 0.52 0.11 54 C -1 -4 -3 -4 10 -3 -4 -3 -3 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.52 0.11 55 K -2 2 0 -2 -4 0 0 -2 8 -4 -3 1 -2 -2 -2 -1 -2 -3 1 -3 0 12 0 0 0 0 0 0 76 0 0 13 0 0 0 0 0 0 0 0 0.85 0.11 56 G 0 -3 -1 -2 -3 -2 -3 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.21 0.11 57 T 0 -2 -1 -2 -2 -2 -2 3 -2 -1 -2 -1 -1 -3 -2 0 4 -3 -2 0 0 0 0 0 0 0 0 35 0 0 0 0 0 0 0 0 53 0 0 12 0.43 0.11 58 S -1 -1 5 0 -2 0 -1 -1 0 -3 -3 -1 -2 -3 -2 2 2 -4 -2 -2 0 0 63 0 0 0 0 0 0 0 0 0 0 0 0 24 13 0 0 0 0.60 0.11 59 N -2 -1 2 5 -4 2 2 -2 -1 -4 -4 0 -3 -4 -2 0 -1 -4 -3 -3 0 0 13 63 0 12 13 0 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.11 60 G 0 -3 -1 -2 -3 -2 -3 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.21 0.11 61 G 2 -2 -1 -2 -2 -2 -2 5 -2 -3 -3 -2 -2 -3 -2 0 -1 -3 -3 -2 31 0 0 0 0 0 0 69 0 0 0 0 0 0 0 0 0 0 0 0 0.80 0.11 62 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.11 63 T -1 4 -1 -2 -2 0 -1 -3 -2 0 -1 2 -1 -2 -2 -1 1 -3 -2 2 0 43 0 0 0 0 0 0 0 0 0 13 0 0 0 0 13 0 0 32 0.33 0.11 64 C -1 -4 -3 -4 10 -3 -4 -3 -3 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.52 0.11 K Lambda Standard Ungapped 0.1344 0.3153 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1542 0.3177 PSI Gapped 0.0472 0.2670