Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 G 0 -3 -1 -1 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 0 -2 -3 -3 -3 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.09 0.03 2 F -2 -3 -3 -4 -2 -3 -4 -4 -2 0 2 -3 0 6 -4 -3 -2 0 2 -1 0 0 0 0 0 0 0 0 0 0 20 0 0 80 0 0 0 0 0 0 0.71 0.08 3 E 1 3 -1 0 -3 1 4 -2 -1 -3 -3 1 -2 -3 -2 0 -1 -3 -2 -2 21 23 0 0 0 0 56 0 0 0 0 0 0 0 0 0 0 0 0 0 0.47 0.08 4 R -1 4 0 -1 -3 4 1 2 0 -3 -3 1 -1 -3 -2 -1 -1 -3 -2 -3 0 36 0 0 0 44 0 20 0 0 0 0 0 0 0 0 0 0 0 0 0.51 0.08 5 T -1 4 0 3 -3 0 0 -2 -1 -2 -2 1 -2 -3 -2 0 3 -3 -2 -2 0 41 0 23 0 0 0 0 0 0 0 0 0 0 0 0 36 0 0 0 0.41 0.08 6 F -2 -3 -3 -4 -3 -3 -3 -4 -1 0 2 -3 0 6 -4 -3 -2 0 4 -1 0 0 0 0 0 0 0 0 0 0 26 0 0 57 0 0 0 0 16 0 0.66 0.12 7 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.22 0.12 8 A 4 -2 -2 -2 -1 -2 -2 4 -2 -3 -3 -1 -2 -3 -2 0 -1 -3 -3 -1 63 0 0 0 0 0 0 37 0 0 0 0 0 0 0 0 0 0 0 0 0.58 0.13 9 F -2 -3 -4 -4 -2 -3 -3 -4 -3 1 3 -3 1 3 -3 -3 -1 -1 0 2 0 0 0 0 0 0 0 0 0 0 53 0 0 23 0 0 0 0 0 24 0.44 0.13 10 P -1 -2 -2 -2 -3 -1 -1 -2 -2 -3 -3 -1 -3 -4 7 2 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 76 24 0 0 0 0 1.55 0.13 11 P 2 -2 -2 -2 -2 -2 -1 -2 -3 -3 -3 -1 -2 -4 7 -1 -1 -4 -3 -2 27 0 0 0 0 0 0 0 0 0 0 0 0 0 73 0 0 0 0 0 1.47 0.13 12 W -3 -3 -4 -5 -3 -2 -3 -3 -3 -3 -2 -4 -2 1 -4 -3 -3 11 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.64 0.13 13 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 14 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 4 -3 2 3 -4 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 86 0 0 14 0 0 0 0 0 0 0.57 0.13 15 E 2 -1 -1 1 -3 1 5 -2 -1 -3 -3 0 -2 -3 -1 0 -1 -3 -2 -2 24 0 0 0 0 0 76 0 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.13 16 R 0 4 0 3 -3 0 2 -2 3 -3 -3 0 -2 -3 -2 -1 -1 -3 -2 -3 14 36 0 24 0 0 14 0 13 0 0 0 0 0 0 0 0 0 0 0 0.43 0.13 17 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -2 -1 3 -3 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.15 0.13 18 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 19 E -1 4 -1 -1 4 0 2 -2 -1 -3 -3 1 -2 -3 -2 1 1 -3 -2 -2 0 37 0 0 14 0 23 0 0 0 0 0 0 0 0 14 13 0 0 0 0.40 0.13 20 E -1 3 0 0 -3 1 4 -2 -1 -3 -3 1 -2 -3 -2 2 0 -3 -2 -3 0 24 0 0 0 0 49 0 0 0 0 0 0 0 0 27 0 0 0 0 0.52 0.13 21 W -3 -3 -4 -5 -3 -2 -3 -3 -3 -3 -2 -4 -2 1 -4 -3 -3 11 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.64 0.13 22 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.24 0.13 23 G 2 -3 -1 -2 -2 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 0 -2 -3 -3 -3 24 0 0 0 0 0 0 76 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.13 24 T 1 3 -1 -1 -2 0 1 -2 -2 0 -1 0 -1 -3 -2 0 2 -3 -2 2 14 26 0 0 0 0 14 0 0 0 0 0 0 0 0 0 23 0 0 24 0.22 0.13 25 R -1 3 -1 -1 -4 1 3 -2 -1 -3 -3 4 -2 -4 -2 -1 -1 -3 -2 -3 0 23 0 0 0 0 24 0 0 0 0 53 0 0 0 0 0 0 0 0 0.59 0.13 26 E 2 -1 -2 0 -2 0 4 -2 -1 -1 1 -1 -1 -2 -2 -1 -1 -3 -2 -1 24 0 0 0 0 0 50 0 0 0 26 0 0 0 0 0 0 0 0 0 0.33 0.13 27 G 2 -2 0 3 -3 -1 1 4 -2 -3 -3 -1 -3 -3 -2 0 -1 -3 -3 -2 26 0 0 24 0 0 14 36 0 0 0 0 0 0 0 0 0 0 0 0 0.48 0.13 28 E -2 -1 0 4 -4 1 5 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 36 0 0 64 0 0 0 0 0 0 0 0 0 0 0 0 0 0.82 0.13 29 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.24 0.13 30 V 0 -2 -2 -2 -1 -2 -2 -2 -2 2 0 -2 0 -1 -2 -1 1 -2 -1 4 2 1 1 1 0 1 1 2 1 1 2 1 1 1 1 2 14 0 1 65 0.26 0.10 31 Y -2 -3 -3 -4 -3 -2 -3 -3 0 -2 -1 -3 -1 4 -4 -2 -2 7 7 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 14 0 0 0 24 62 0 1.13 0.13 32 R 1 6 -1 -2 -3 0 -1 -2 -1 -3 -2 1 -2 -3 -2 0 1 -3 -2 -2 14 74 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13 0 0 0 0.63 0.13 33 L -2 -3 -4 -4 -2 -3 -3 -4 -3 2 4 -3 1 0 -3 -3 -1 -2 -2 2 0 0 0 0 0 0 0 0 0 0 76 0 0 0 0 0 0 0 0 24 0.52 0.13 34 P -1 -1 -1 3 -4 -1 0 -2 -2 -3 -4 1 -3 -4 7 -1 -1 -4 -3 -3 0 0 0 24 0 0 0 0 0 0 0 14 0 0 63 0 0 0 0 0 1.27 0.13 35 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.24 0.13 36 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.59 0.13 37 V -2 4 -2 -3 -3 -1 -2 -3 -2 -1 -1 0 -1 -1 -3 -2 -2 9 0 1 0 40 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 37 0 23 0.86 0.13 38 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.56 0.13 39 R -2 6 -1 -2 -4 1 0 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.13 40 F -3 -3 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.02 0.13 41 R 2 5 -1 -2 -3 0 -1 -2 -1 -2 -2 1 -1 2 -2 -1 -1 -2 -1 -2 27 59 0 0 0 0 0 0 0 0 0 0 0 14 0 0 0 0 0 0 0.46 0.13 42 E 2 -1 -1 1 -3 1 5 -2 -1 -3 -3 0 -2 -3 -1 0 -1 -3 -2 -2 24 0 0 0 0 0 76 0 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.13 43 V 2 -3 -3 -3 -1 -2 -2 -2 -3 1 2 -2 0 -1 -2 -1 -1 -3 -2 3 27 0 0 0 0 0 0 0 0 0 24 0 0 0 0 0 0 0 0 49 0.31 0.13 44 E -1 -1 -1 0 -4 1 5 -3 -1 -4 -3 0 -3 -4 5 -1 -1 -4 -3 -3 0 0 0 0 0 0 62 0 0 0 0 0 0 0 38 0 0 0 0 0 0.98 0.13 45 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.20 0.13 46 L -2 2 -3 -4 -2 -1 -2 -4 -2 1 4 -1 1 0 -3 -2 -2 -2 -2 0 0 24 0 0 0 0 0 0 0 0 76 0 0 0 0 0 0 0 0 0 0.44 0.13 47 K -1 2 -1 -1 -3 2 2 -3 -1 -1 -2 3 -1 -3 -2 -1 -1 -3 -2 1 0 14 0 0 0 14 14 0 0 0 0 35 0 0 0 0 0 0 0 24 0.32 0.13 48 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.56 0.13 49 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.24 0.13 50 S 0 1 0 -1 -2 -1 -1 3 -1 -3 -3 0 -2 -3 -2 4 0 -3 -2 -3 0 14 0 0 0 0 0 24 0 0 0 0 0 0 0 63 0 0 0 0 0.46 0.13 51 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 52 S 0 4 0 -1 -2 0 -1 -1 -1 -3 -3 1 -2 -3 -2 4 0 -3 -2 -2 0 38 0 0 0 0 0 0 0 0 0 0 0 0 0 62 0 0 0 0 0.51 0.13 53 I -1 -3 -3 -3 -2 -2 -3 -4 -3 4 2 -2 1 -1 -3 -1 2 -3 -2 2 0 0 0 0 0 0 0 0 0 49 14 0 0 0 0 0 24 0 0 14 0.36 0.13 54 P -1 -2 -2 -2 -3 -1 -1 -2 -2 -3 -3 -1 -3 -4 7 2 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 76 24 0 0 0 0 1.55 0.13 55 Q -2 5 -1 -1 -4 5 1 -3 0 -3 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 51 0 0 0 49 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.13 56 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 57 R -2 4 -1 -1 -3 2 2 -3 -1 -2 0 1 -1 2 -2 -1 -2 -2 -1 -2 0 39 0 0 0 14 24 0 0 0 11 0 0 13 0 0 0 0 0 0 0.37 0.13 58 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.56 0.13 59 E -1 0 -1 1 -4 2 6 -3 0 -4 -3 0 -2 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 60 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.56 0.13 61 E -1 -1 -1 1 -4 1 5 2 -1 -4 -4 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 76 24 0 0 0 0 0 0 0 0 0 0 0 0 0.70 0.13 62 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.24 0.13 63 E 2 -1 -1 1 -3 1 5 -2 -1 -3 -3 0 -2 -3 -1 0 -1 -3 -2 -2 24 0 0 0 0 0 76 0 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.13 64 E 2 -1 -1 1 -3 1 5 -2 -1 -3 -3 0 -2 -3 -1 0 -1 -3 -2 -2 25 0 0 0 0 0 75 0 0 0 0 0 0 0 0 0 0 0 0 0 0.64 0.13 65 A 4 -2 -2 -2 -1 -1 -2 3 -2 -2 -2 -1 -2 -3 -2 1 -1 -3 -3 -1 75 0 0 0 0 0 0 25 0 0 0 0 0 0 0 0 0 0 0 0 0.54 0.13 66 E -1 3 -1 1 -4 1 5 -3 -1 -4 -3 1 -2 -4 -2 -1 -1 -3 -2 -3 0 24 0 0 0 0 76 0 0 0 0 0 0 0 0 0 0 0 0 0 0.75 0.13 67 R 3 4 -1 -2 -2 0 -1 -1 -1 -2 -2 2 -2 -3 -2 0 -1 -3 -2 -1 52 35 0 0 0 0 0 0 0 0 0 13 0 0 0 0 0 0 0 0 0.42 0.13 68 W -3 -3 -4 -5 -3 -2 -3 -3 -3 -3 -2 -4 -2 1 -4 -3 -3 11 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.64 0.13 69 F 2 -3 -3 -3 -2 -2 -3 -2 -2 -1 -1 -2 -1 5 -3 -1 -2 6 2 -1 37 0 0 0 0 0 0 0 0 0 0 0 0 49 0 0 0 14 0 0 0.56 0.13 70 E -1 -1 -1 0 -4 2 4 3 -1 -4 -3 0 -2 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 14 59 27 0 0 0 0 0 0 0 0 0 0 0 0 0.62 0.13 71 R -2 6 -1 -2 -4 1 0 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.13 72 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.62 0.13 73 A 4 -1 -1 -2 -1 2 -1 -1 -2 -2 -2 -1 -1 -3 -1 2 0 -3 -2 -1 63 0 0 0 0 14 0 0 0 0 0 0 0 0 0 24 0 0 0 0 0.42 0.13 74 F -2 -3 -4 -4 -2 -3 -3 -4 -3 1 4 -3 1 2 -3 -3 -1 -2 -1 2 0 0 0 0 0 0 0 0 0 0 65 0 0 11 0 0 0 0 0 24 0.46 0.13 75 A 4 -2 -2 -2 -1 -1 -2 3 -2 -2 -2 -1 -2 -3 -2 1 -1 -3 -3 -1 76 0 0 0 0 0 0 24 0 0 0 0 0 0 0 0 0 0 0 0 0.54 0.13 76 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.59 0.13 77 S 2 -1 0 -1 -1 -1 -1 -1 -2 -1 -1 -1 3 -2 -2 4 1 -3 -2 -1 14 0 0 0 0 0 0 0 0 0 0 0 24 0 0 63 0 0 0 0 0.36 0.13 78 R -2 6 -1 -2 -4 1 0 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.13 K Lambda Standard Ungapped 0.1418 0.3207 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1599 0.3195 PSI Gapped 0.0490 0.2670