Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 I -2 -3 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.54 0.09 2 E 0 -1 0 3 -4 1 5 -2 -1 -4 -3 0 -3 -4 -2 0 -1 -4 -3 -3 10 0 0 22 0 0 69 0 0 0 0 0 0 0 0 0 0 0 0 0 0.72 0.14 3 E -1 -1 -1 0 -3 1 5 -3 -1 1 -2 0 -2 -3 2 -1 -1 -3 -3 -1 0 0 0 0 0 0 67 0 0 21 0 0 0 0 12 0 0 0 0 0 0.53 0.14 4 E -2 -1 0 4 -4 1 5 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 33 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0.83 0.14 5 L -2 -3 -4 -4 -2 -2 -3 -4 -3 1 4 -3 1 -1 4 -3 -2 -3 -2 0 0 0 0 0 0 0 0 0 0 0 79 0 0 0 21 0 0 0 0 0 0.60 0.14 6 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.66 0.14 7 L -2 2 -2 -3 -2 3 -1 -4 -2 1 3 -1 1 -1 -3 -2 -2 -2 -2 0 0 16 0 0 0 21 0 0 0 6 57 0 0 0 0 0 0 0 0 0 0.32 0.14 8 Q -1 1 0 -1 -4 7 2 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.14 9 Q -1 1 0 -1 -4 7 2 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.14 10 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 2 -3 1 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 92 8 0 0 0 0 0 0 0 0 0 0.60 0.14 11 D -2 -2 2 6 -4 -1 1 -2 -1 -3 -3 -1 -3 2 -2 -1 -2 -3 -1 -3 0 0 8 73 0 0 0 0 0 0 0 0 0 19 0 0 0 0 0 0 0.75 0.14 12 N -2 -1 6 0 -3 -1 -1 -1 0 1 -2 -1 -2 -3 -3 0 -1 -4 -2 -1 0 0 81 0 0 0 0 0 0 19 0 0 0 0 0 0 0 0 0 0 0.65 0.14 13 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 92 0 0 0 0 0 0 0 0 0 8 0.61 0.14 14 K -1 4 -1 -1 -4 1 0 -2 -1 -3 -3 5 -2 -4 -2 -1 -1 -3 -2 -3 0 37 0 0 0 0 0 0 0 0 0 63 0 0 0 0 0 0 0 0 0.71 0.14 15 A 4 -2 -2 -2 -1 -1 -2 3 -2 -2 -2 -1 -2 -3 -2 1 -1 -3 -3 -1 81 0 0 0 0 0 0 19 0 0 0 0 0 0 0 0 0 0 0 0 0.55 0.14 16 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -2 -1 -2 -1 4 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 91 0 1.10 0.14 17 I -2 -3 -4 -4 -2 -3 -4 -4 -4 5 2 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 81 19 0 0 0 0 0 0 0 0 0 0.56 0.14 18 F -2 -1 -3 -3 -3 -2 -2 -3 -2 -1 0 1 -1 7 -4 -2 -2 0 2 -2 0 0 0 0 0 0 0 0 0 0 0 19 0 81 0 0 0 0 0 0 0.72 0.14 19 D -2 -2 1 6 -4 0 3 -2 -1 -4 -4 -1 -3 -4 -2 -1 -1 -5 -3 -4 0 0 0 81 0 0 19 0 0 0 0 0 0 0 0 0 0 0 0 0 1.00 0.14 20 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.61 0.14 21 K 1 1 -1 -1 -3 1 0 -2 -1 -3 -3 5 -2 -4 -1 0 -1 -3 -2 -2 19 0 0 0 0 0 0 0 0 0 0 81 0 0 0 0 0 0 0 0 0.56 0.14 22 Q -1 0 -1 -1 -3 5 3 -3 4 -3 -1 0 -1 -3 -2 -1 -1 -3 -1 -2 0 0 0 0 0 51 19 0 20 0 10 0 0 0 0 0 0 0 0 0 0.54 0.14 23 C -1 -2 1 2 6 -1 -1 1 2 -3 -3 -2 -2 -3 -2 2 -1 -3 0 -2 0 0 4 19 29 0 0 9 8 0 0 0 0 0 0 27 0 0 4 0 0.42 0.14 24 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.25 0.14 25 R -2 7 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.00 0.14 26 L -1 -2 -3 -3 -2 -2 -3 -3 -3 1 4 -2 3 0 -3 0 0 -2 -2 0 4 0 0 0 0 0 0 0 0 0 74 0 10 0 0 9 4 0 0 0 0.42 0.14 27 D -2 -2 1 7 -4 0 2 -2 -1 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 91 0 0 9 0 0 0 0 0 0 0 0 0 0 0 0 0 1.08 0.14 28 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.94 0.14 29 V -1 -3 -4 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.61 0.16 30 E 0 -1 -1 2 -4 1 5 -2 -1 -4 -3 0 -3 -4 -2 0 -1 -4 -3 -3 10 0 0 11 0 0 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.16 31 V 0 -3 -3 -4 -1 -3 -3 -3 -4 3 2 -3 0 -1 -3 -2 -1 -3 -2 4 10 0 0 0 0 0 0 0 0 17 14 0 0 0 0 0 0 0 0 59 0.42 0.16 32 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.69 0.16 33 T 0 -1 -1 -1 -2 0 2 -2 -2 -2 -2 0 -1 -3 -2 1 5 -3 -2 -1 4 0 0 0 0 0 17 0 0 0 0 6 0 0 0 5 69 0 0 0 0.49 0.16 34 E -1 -1 -1 1 -4 3 5 2 -1 -4 -3 0 -2 -4 -2 -1 -1 -3 -3 -3 0 0 0 4 0 22 58 17 0 0 0 0 0 0 0 0 0 0 0 0 0.64 0.16 35 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 -1 -4 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.03 0.16 36 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.69 0.16 37 R -2 6 -1 -2 -4 0 -1 -1 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 89 0 0 0 0 0 6 0 0 0 4 0 0 0 0 0 0 0 0 0.90 0.16 38 E -1 -1 -1 3 -4 1 5 -3 -1 -3 -3 0 -2 -4 -2 -1 -1 -4 -3 -1 0 0 0 17 0 0 77 0 0 0 0 0 0 0 0 0 0 0 0 6 0.77 0.16 39 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.69 0.16 40 K -1 3 -1 -1 -4 5 1 -2 -1 -3 -3 4 -1 -4 -2 -1 -1 -3 -2 -3 0 15 0 0 0 44 0 0 0 0 0 42 0 0 0 0 0 0 0 0 0.65 0.16 41 H -2 3 2 -1 3 2 -1 -2 4 -1 -1 0 -1 -3 -3 0 -1 -3 -1 -2 0 28 17 0 11 10 0 0 20 6 5 0 0 0 0 3 0 0 0 0 0.35 0.16 42 T 1 -2 -1 -1 -2 -1 2 -2 -2 -1 -2 -1 -1 -3 -2 1 5 -3 -2 0 14 0 0 0 0 0 17 0 0 0 0 0 0 0 0 0 64 0 0 6 0.44 0.16 43 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.69 0.16 44 A 4 -1 -2 -2 -1 1 -1 -1 -2 -2 -2 2 -1 -3 -1 0 -1 -3 -2 -1 74 0 0 0 0 6 0 0 0 0 0 19 0 0 0 0 0 0 0 0 0.44 0.14 45 K -1 3 -1 -1 -3 1 2 -3 -1 -1 -2 4 -1 -3 -2 -1 -1 -3 -2 1 0 24 0 0 0 0 19 0 0 0 0 38 0 0 0 0 0 0 0 19 0.38 0.14 46 Q -1 0 0 -1 -4 6 1 -2 4 -3 -3 1 -1 -3 -2 -1 -1 -3 -1 -3 0 0 0 0 0 81 0 0 19 0 0 0 0 0 0 0 0 0 0 0 0.79 0.14 47 K -1 1 -1 -1 -4 1 0 2 -1 -3 -3 5 -2 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 5 0 19 0 0 0 76 0 0 0 0 0 0 0 0 0.58 0.14 48 G 0 1 -1 -2 -3 -2 -2 6 -2 -4 -4 -1 -3 -4 -3 -1 -2 -3 -3 -3 7 10 0 0 0 0 0 83 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.12 49 G 1 -3 -1 -2 1 -2 -2 6 -3 -4 -4 -2 -3 -3 -2 0 -2 -3 -3 -3 15 0 0 0 5 0 0 79 0 0 0 0 0 0 0 0 0 0 0 0 0.91 0.12 50 T 1 -2 -1 -2 -2 -1 -1 -2 -2 -2 -2 -1 -2 -3 6 0 3 -3 -3 -1 19 0 0 0 0 0 0 0 0 0 0 0 0 0 44 0 37 0 0 0 0.79 0.07 51 D -2 -2 2 6 -4 0 2 -2 -1 -4 -4 -1 -3 -4 -2 0 -1 -4 -3 -3 0 0 10 79 0 0 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.06 K Lambda Standard Ungapped 0.1361 0.3147 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1612 0.3163 PSI Gapped 0.0493 0.2670