Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -2 -2 -3 -4 -2 -1 -3 -3 -2 1 2 -2 8 -1 -3 -2 -1 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.79 0.18 2 F -3 -4 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 -1 7 -4 -3 -3 5 3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 93 0 0 0 7 0 0 1.11 0.18 3 E -1 -1 -1 1 -5 2 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 1.01 0.18 4 K -2 4 -1 -2 -4 1 0 -2 -1 -4 -3 5 -2 -4 -2 -1 -1 -4 -2 -3 0 31 0 0 0 0 0 0 0 0 0 69 0 0 0 0 0 0 0 0 0.76 0.18 5 I -2 -4 -4 -4 -2 -3 -4 -5 -4 5 3 -3 1 -1 -4 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 69 31 0 0 0 0 0 0 0 0 0 0.60 0.18 6 R -2 2 3 5 -4 0 3 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -4 0 14 18 38 0 0 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0.66 0.18 7 K -1 -1 -2 -1 -3 1 3 1 -2 -1 2 1 0 -2 -2 0 -1 -3 -2 -1 0 0 0 0 0 7 25 12 0 0 37 14 0 0 0 6 0 0 0 0 0.21 0.18 8 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 1 -3 1 -1 -4 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.72 0.18 9 L -2 -3 -4 -4 -2 -3 -4 -4 -4 4 3 -3 1 2 -4 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 50 37 0 0 13 0 0 0 0 0 0 0.52 0.18 10 A 2 3 -1 -2 -3 1 0 -2 -1 -3 -3 4 -2 -4 -2 0 -1 -4 -2 -2 25 17 0 0 0 6 0 0 0 0 0 51 0 0 0 0 0 0 0 0 0.49 0.18 11 D -2 0 5 4 -4 0 2 0 -1 -4 -4 -1 -3 -4 -2 1 -1 -4 -3 -4 0 6 41 28 0 0 12 6 0 0 0 0 0 0 0 7 0 0 0 0 0.60 0.18 12 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 1 -3 1 -1 -4 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.72 0.18 13 E -1 -1 -1 1 -5 2 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 1.01 0.18 14 D -1 -1 5 4 -4 0 3 -2 -1 -4 -4 -1 -3 -4 -2 0 -1 -4 -3 -3 6 0 38 27 0 0 29 0 0 0 0 0 0 0 0 0 0 0 0 0 0.66 0.18 15 S 3 -2 -2 -2 -1 -2 -2 -2 -2 3 -1 -2 -1 -2 -2 2 1 -3 -2 0 33 0 0 0 0 0 0 0 0 31 0 0 0 0 0 29 7 0 0 0 0.29 0.18 16 Q -2 4 -1 -1 -4 5 1 -3 -1 -4 -3 4 -2 -4 -2 -1 -1 -3 -2 -3 0 30 0 0 0 39 0 0 0 0 0 31 0 0 0 0 0 0 0 0 0.68 0.18 17 N 1 -1 1 4 -3 0 4 -2 -1 -3 -4 -1 -3 -4 -2 1 -1 -4 -3 -3 18 0 6 31 0 0 37 0 0 0 0 0 0 0 0 7 0 0 0 0 0.55 0.18 18 E -2 -1 -1 3 -4 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 13 0 0 87 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.18 19 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 1 -3 1 -1 -4 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 92 0 0 0 0 0 0 0 0 0 8 0.68 0.18 20 E -1 0 2 0 -4 3 4 -2 -1 -3 -3 1 -2 -4 -2 0 1 -4 -3 -3 0 0 13 0 0 18 51 0 0 0 0 6 0 0 0 0 12 0 0 0 0.58 0.18 21 M -2 -3 -4 -4 -2 -3 -4 -4 -4 4 3 -3 3 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 37 32 0 14 0 0 0 0 0 0 17 0.49 0.18 22 L -1 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 7 0 0 0 0 0 0 0 0 0 93 0 0 0 0 0 0 0 0 0 0.63 0.18 23 L -2 -3 -4 -4 -2 -3 -4 -5 -4 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.73 0.18 24 K -1 2 3 -1 -4 0 -1 3 -1 -4 -4 3 -3 -4 -2 -1 -1 -4 -3 -3 0 8 26 0 0 0 0 29 0 0 0 37 0 0 0 0 0 0 0 0 0.52 0.18 25 L -2 -3 -4 -4 -2 -2 -3 -4 -3 4 3 -3 5 -1 -3 -3 -1 -3 -2 1 0 0 0 0 0 0 0 0 0 44 19 0 36 0 0 0 0 0 0 0 0.54 0.18 26 A 5 -2 -2 -2 -1 -1 -1 1 -2 -2 -2 -1 -2 -3 -1 1 -1 -3 -2 -1 93 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0.59 0.15 27 N -2 1 5 0 -4 0 0 -2 3 -4 -4 4 -2 -3 -2 0 -1 -4 -2 -3 0 0 43 0 0 0 0 0 12 0 0 45 0 0 0 0 0 0 0 0 0.57 0.15 28 L -2 -3 -4 -4 -2 -3 -4 -4 -4 5 3 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 68 25 0 0 0 0 0 0 0 0 7 0.55 0.15 29 S 1 -1 2 -1 -2 -1 -1 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -4 -2 -2 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 92 0 0 0 0 0.62 0.15 30 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 4 -3 1 4 -4 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 70 0 0 30 0 0 0 0 0 0 0.59 0.15 31 G 0 -3 -1 -2 -2 -2 -1 4 -3 1 -1 -2 -1 -2 -2 1 -1 -3 -3 2 0 0 0 0 0 0 5 36 0 5 0 0 0 0 0 19 0 0 0 35 0.31 0.15 32 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.19 0.15 33 F -3 -3 -3 -4 -3 -3 -3 -4 0 -1 -1 -3 -1 6 -4 -3 -3 1 6 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 55 0 0 0 0 45 0 0.95 0.15 34 I -2 -4 -4 -4 -2 -3 -4 -5 -4 6 1 -3 1 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.66 0.15 35 E -2 -2 -3 -3 -2 -1 1 -4 -2 0 2 -2 6 3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 13 0 0 0 24 0 45 18 0 0 0 0 0 0 0.39 0.15 36 I -2 -3 -2 2 -2 -2 -2 -4 -3 4 1 -3 4 -1 -3 -2 -1 -3 -2 1 0 0 0 18 0 0 0 0 0 58 0 0 24 0 0 0 0 0 0 0 0.38 0.15 37 K -1 2 -1 -1 -4 1 2 -2 -1 -3 -3 5 -2 -4 -2 -1 -1 -4 -2 -3 0 0 0 0 0 0 18 0 0 0 0 82 0 0 0 0 0 0 0 0 0.68 0.15 38 R -2 6 -1 -2 -3 0 -1 -3 -1 -3 -3 1 -2 -3 -3 -1 2 -3 -2 -3 0 82 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 0 0 0 0.79 0.15 39 G 0 -3 -1 -2 -3 -2 -3 7 -3 -5 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.28 0.15 40 S 1 -1 0 0 -2 0 2 -1 -1 -3 -3 0 -2 -3 -1 5 1 -3 -2 -2 6 0 0 0 0 0 18 0 0 0 0 0 0 0 0 76 0 0 0 0 0.53 0.15 41 M -2 -1 -2 -3 -2 3 -1 -3 -2 0 3 -1 5 -1 -3 -2 -1 -2 -2 0 0 0 0 0 0 24 0 0 0 0 41 0 35 0 0 0 0 0 0 0 0.40 0.15 42 D -2 2 1 6 -4 0 1 -2 -1 -4 -4 0 -3 -4 -2 -1 -2 -5 -3 -4 0 18 0 82 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.94 0.15 43 M -2 -2 -2 -3 -2 -1 -3 -4 4 3 1 -2 6 -1 -3 -2 -1 -3 -1 1 0 0 0 0 0 0 0 0 18 35 0 0 47 0 0 0 0 0 0 0 0.43 0.15 44 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -3 -4 -2 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.23 0.15 45 K -1 -1 0 2 -4 1 5 -2 -1 -4 -4 1 -3 -4 2 1 -1 -4 -3 -3 0 0 0 12 0 0 63 0 0 0 0 7 0 0 8 10 0 0 0 0 0.63 0.13 46 G -1 -2 0 -1 -3 -1 0 3 5 -3 -3 -1 -2 -3 -2 1 2 -3 -1 -3 0 0 0 0 0 0 7 33 26 0 0 0 0 0 0 11 23 0 0 0 0.46 0.16 47 V -2 -3 -4 -4 -2 -3 -3 -4 -3 1 4 -3 3 0 -3 -3 -1 -2 -2 2 0 0 0 0 0 0 0 0 0 0 73 0 8 0 0 0 0 0 0 19 0.54 0.16 48 N -1 -1 5 3 -3 1 0 3 -1 -4 -4 -1 -3 -4 -2 1 0 -4 -3 -3 0 0 40 17 0 8 0 22 0 0 0 0 0 0 0 6 7 0 0 0 0.51 0.16 49 E -1 -1 -2 -1 -3 0 3 -3 -2 0 1 -1 6 -1 -3 -2 -1 -3 -2 1 0 0 0 0 0 0 33 0 0 0 0 0 56 0 0 0 0 0 0 10 0.40 0.14 50 A 4 -2 -2 -2 -1 -1 -1 -1 -2 -2 -2 -1 -2 -2 -2 2 0 6 -1 -1 62 0 0 0 0 0 0 0 0 0 0 0 0 0 0 24 0 14 0 0 0.53 0.15 51 F -2 -3 1 -3 -3 -3 -3 -3 -2 0 1 -3 0 6 -4 -2 -2 0 2 1 0 0 14 0 0 0 0 0 0 0 11 0 0 62 0 0 0 0 0 13 0.53 0.15 52 F -3 -3 -3 -4 -3 -3 -3 -4 0 -1 1 -3 -1 5 -4 -3 -2 7 6 -1 0 0 0 0 0 0 0 0 0 0 16 0 0 27 0 0 0 16 42 0 0.83 0.15 53 T 1 -1 -1 -1 -2 2 2 -2 -1 -2 -2 0 -1 -3 -2 1 4 -3 -2 -1 14 0 0 0 0 16 16 0 0 0 0 0 0 0 0 0 55 0 0 0 0.42 0.15 54 Q 1 0 -1 -1 -3 6 1 -2 4 -3 -3 0 -1 -3 -2 0 -1 -3 -1 -3 14 0 0 0 0 71 0 0 16 0 0 0 0 0 0 0 0 0 0 0 0.65 0.15 55 L -2 -3 -4 -4 -2 -3 -4 -4 -4 4 3 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 52 32 0 0 0 0 0 0 0 0 16 0.51 0.15 56 S -1 -2 3 4 -3 -1 0 4 -2 -4 -4 -1 -3 -4 -2 1 -1 -4 -3 -4 0 0 16 32 0 0 0 36 0 0 0 0 0 0 0 16 0 0 0 0 0.59 0.15 57 E -1 -1 -1 1 -4 1 6 -3 -1 -3 -3 0 -2 -4 -2 0 1 -3 -3 -3 0 0 0 0 0 0 84 0 0 0 0 0 0 0 0 0 16 0 0 0 0.77 0.15 58 E -2 3 -1 0 -4 3 4 -3 3 -4 -3 1 -2 -4 -2 -1 -2 -3 -2 -3 0 25 0 0 0 16 47 0 12 0 0 0 0 0 0 0 0 0 0 0 0.61 0.15 59 V -1 -3 -4 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.60 0.15 60 E 1 -1 -1 3 -3 1 4 -2 -1 -2 -2 0 3 -3 -2 -1 -1 -3 -3 -2 16 0 0 16 0 0 52 0 0 0 0 0 16 0 0 0 0 0 0 0 0.46 0.15 61 R 1 3 -1 0 -3 1 4 -2 -1 -3 -3 3 -2 -4 -2 0 -1 -4 -2 -3 16 16 0 0 0 0 36 0 0 0 0 31 0 0 0 0 0 0 0 0 0.47 0.15 62 L -2 -3 -4 -4 -2 -3 -4 -4 -4 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.68 0.15 63 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.77 0.15 64 E 1 0 -1 2 -3 1 5 -2 -1 -3 -3 2 -2 -4 -2 0 -1 -4 -3 -3 16 0 0 13 0 0 55 0 0 0 0 16 0 0 0 0 0 0 0 0 0.55 0.15 65 L 0 -1 -1 -1 -3 2 2 -2 6 -2 0 -1 -1 -2 -2 -1 -2 -3 0 -2 13 0 0 0 0 16 16 0 39 0 16 0 0 0 0 0 0 0 0 0 0.42 0.15 66 I -2 -4 -4 -4 -2 -3 -4 -5 -4 6 1 -3 1 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.66 0.15 67 N -2 -1 5 4 -4 0 2 -1 0 -4 -4 -1 -3 -4 -2 0 -1 -5 -3 -4 0 0 52 32 0 0 16 0 0 0 0 0 0 0 0 0 0 0 0 0 0.75 0.15 68 A 4 -2 -3 -3 -1 -2 -2 -1 -3 0 1 -2 0 -2 -2 0 -1 -3 -2 1 68 0 0 0 0 0 0 0 0 0 16 0 0 0 0 0 0 0 0 16 0.36 0.15 69 L -2 -3 -4 -4 -2 -3 -4 -4 -4 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.68 0.15 70 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 -1 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.02 0.15 71 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.77 0.15 72 I -2 -3 -4 -4 -2 -3 -3 -4 -1 3 3 -3 1 1 -4 -3 -2 0 5 1 0 0 0 0 0 0 0 0 0 31 32 0 0 0 0 0 0 0 36 0 0.49 0.15 73 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.77 0.15 74 K -2 5 -1 -2 -4 1 0 -3 -1 -4 -3 4 -2 -4 -2 -1 -1 -4 -2 -3 0 52 0 0 0 0 0 0 0 0 0 48 0 0 0 0 0 0 0 0 0.76 0.15 75 G -1 -1 -1 1 -4 1 6 1 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 84 16 0 0 0 0 0 0 0 0 0 0 0 0 0.77 0.15 76 L -2 -2 -3 -4 -2 -2 -3 -4 -3 1 2 -3 6 0 -3 -2 -1 6 -1 2 0 0 0 0 0 0 0 0 0 0 16 0 52 0 0 0 0 16 0 16 0.59 0.15 77 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 1 3 -4 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 84 0 0 16 0 0 0 0 0 0 0.60 0.15 78 V -1 -3 -4 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -4 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.56 0.13 79 F -3 -3 -3 -4 -3 -3 -4 -4 -1 -1 0 -3 -1 7 -4 -3 -3 1 5 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 80 0 0 0 0 20 0 0.94 0.13 K Lambda Standard Ungapped 0.1390 0.3192 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1683 0.3166 PSI Gapped 0.0515 0.2670