Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 Q 0 -1 0 2 -3 3 2 -2 -1 -3 -3 0 -2 -4 -2 4 0 -4 -3 -3 0 0 0 13 0 20 17 0 0 0 0 0 0 0 0 50 0 0 0 0 0.53 0.24 2 R -2 7 -1 -2 -4 0 -1 -3 -1 -4 -3 2 -2 -4 -3 -1 -2 -4 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.17 0.24 3 E 0 5 -1 2 -4 0 1 1 -1 -4 -3 1 -2 -4 -2 -1 -2 -4 -3 -3 13 52 0 15 0 0 11 10 0 0 0 0 0 0 0 0 0 0 0 0 0.54 0.24 4 N 1 -2 6 0 -3 -1 -1 1 -1 -3 -4 -1 -3 -4 -2 0 2 -4 -3 -3 13 0 60 0 0 0 0 12 0 0 0 0 0 0 0 0 15 0 0 0 0.65 0.24 5 L -2 -3 -4 -4 -2 -3 -4 -5 -4 2 5 -3 1 0 -4 -3 -2 -3 -2 0 0 0 0 0 0 0 0 0 0 12 88 0 0 0 0 0 0 0 0 0 0.73 0.24 6 T -1 -1 -1 -1 -2 2 1 1 4 -3 -3 -1 -2 -3 -2 2 3 -3 -2 -2 0 0 0 0 0 11 12 9 15 0 0 0 0 0 0 21 33 0 0 0 0.39 0.24 7 T 5 -2 -2 -2 -1 -1 -2 -1 -2 -2 -2 -1 -2 -3 -2 1 2 -3 -3 -1 80 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 20 0 0 0 0.61 0.24 8 G -1 2 -1 -2 -3 -2 -2 6 -2 -5 -4 -1 -3 -4 -3 -1 -2 -3 -4 -4 0 15 0 0 0 0 0 85 0 0 0 0 0 0 0 0 0 0 0 0 1.11 0.24 9 H 3 -1 2 -2 -2 -1 -1 -1 7 -3 -3 -1 -2 -3 -2 1 -1 -3 0 -2 36 0 11 0 0 0 0 0 45 0 0 0 0 0 0 9 0 0 0 0 0.63 0.24 10 D -1 -3 -1 4 -3 -1 1 -3 -3 0 -1 -2 -1 -3 -3 -2 -1 -4 -3 4 0 0 0 36 0 0 11 0 0 0 0 0 0 0 0 0 0 0 0 54 0.47 0.24 11 G 0 -3 -1 -2 -3 -3 -3 6 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.40 0.24 12 D -2 1 2 6 -4 -1 1 -2 -1 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 12 11 77 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.01 0.24 13 V -1 -3 -4 -4 -2 -3 -3 -4 -4 2 2 -3 0 -1 -3 -3 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 0 22 0 0 0 0 0 0 0 0 78 0.62 0.24 14 L -2 -3 -4 -5 -2 -3 -4 -5 -4 1 5 -3 1 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.80 0.24 15 W -3 -4 -5 -5 -3 -3 -4 -3 -3 1 -2 -4 -2 0 -4 -4 -3 11 1 -2 0 0 0 0 0 0 0 0 0 15 0 0 0 0 0 0 0 85 0 0 2.32 0.24 16 V -1 -3 -4 -4 -2 -3 -3 -4 -4 2 0 -3 0 -2 -3 -2 -1 -4 -2 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.74 0.24 17 R -2 7 -1 -2 -4 0 -1 -3 3 -4 -3 1 -2 -3 -3 -1 -2 -3 -2 -3 0 89 0 0 0 0 0 0 11 0 0 0 0 0 0 0 0 0 0 0 1.05 0.24 18 T -2 2 -1 -2 -3 -1 -1 -3 7 -3 -3 -1 -2 -2 -3 -1 2 6 0 -3 0 15 0 0 0 0 0 0 52 0 0 0 0 0 0 0 20 13 0 0 0.81 0.24 19 V 3 -1 -2 -2 -2 3 -1 -2 -2 -1 -2 1 -1 -3 -2 0 1 -3 -2 2 33 0 0 0 0 24 0 0 0 0 0 11 0 0 0 0 12 0 0 20 0.30 0.24 20 T 1 -2 -1 2 -2 -1 -1 -2 -2 -2 -2 -1 -2 -3 -2 2 5 -4 -3 -1 13 0 0 15 0 0 0 0 0 0 0 0 0 0 0 12 60 0 0 0 0.54 0.24 21 G 2 -2 -1 -1 -3 1 2 3 -2 -3 -3 -1 -3 -4 4 1 -1 -4 -3 -3 20 0 0 0 0 9 15 22 0 0 0 0 0 0 25 10 0 0 0 0 0.54 0.24 22 A 3 -2 -1 2 -2 2 0 -1 4 -2 -2 -1 -2 -3 -2 0 -1 -4 -2 0 45 0 0 19 0 12 0 0 15 0 0 0 0 0 0 0 0 0 0 9 0.37 0.24 23 G 0 -3 -1 -2 -3 -3 -3 6 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.40 0.24 24 E 0 -1 -1 0 -3 1 5 -2 -1 -3 -3 1 -2 -4 -2 0 3 -4 -3 -2 9 0 0 0 0 0 55 0 0 0 0 10 0 0 0 0 27 0 0 0 0.57 0.24 25 R -2 7 -1 -2 -4 0 -1 -3 -1 -4 -3 2 -2 -4 -3 -1 -2 -4 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.17 0.24 26 V -1 -3 -3 -4 -2 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 1 -3 -2 5 0 0 0 0 0 0 0 0 0 0 12 0 0 0 0 0 11 0 0 78 0.56 0.24 27 L -2 -3 -4 -5 -2 -3 -4 -5 -4 1 5 -3 1 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.80 0.24 28 L -2 -3 -4 -4 -2 -3 -4 -4 -4 1 5 -3 1 0 -4 -3 -2 -3 -2 2 0 0 0 0 0 0 0 0 0 0 85 0 0 0 0 0 0 0 0 15 0.69 0.24 29 W -3 -4 -5 -5 -3 -3 -4 -3 -3 -3 -2 -4 -2 0 -5 -4 -3 12 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.89 0.24 30 N -2 -1 7 0 -4 -1 -1 -1 4 -4 -4 -1 -3 -4 -3 0 -1 -4 -2 -4 0 0 88 0 0 0 0 0 12 0 0 0 0 0 0 0 0 0 0 0 1.05 0.24 31 L -1 -3 -3 -4 -2 -3 -3 -4 -3 1 3 -3 1 -1 -3 -2 2 -3 -2 3 0 0 0 0 0 0 0 0 0 0 48 0 0 0 0 0 24 0 0 28 0.43 0.24 32 G 1 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -3 13 0 0 0 0 0 0 87 0 0 0 0 0 0 0 0 0 0 0 0 1.18 0.24 33 Q 1 1 -1 -1 -3 4 3 2 3 -4 -3 0 -2 -4 -2 -1 -2 -3 -2 -3 13 9 0 0 0 30 22 15 12 0 0 0 0 0 0 0 0 0 0 0 0.45 0.24 34 D 2 -2 -1 2 -3 2 3 2 -2 -2 -3 -1 -2 -3 -2 -1 -1 -4 -3 0 21 0 0 11 0 10 31 15 0 0 0 0 0 0 0 0 0 0 0 13 0.33 0.24 35 T 3 -2 -2 1 -2 -2 -1 -1 -2 -3 -3 -2 -2 -3 5 0 1 -4 -3 -2 50 0 0 12 0 0 0 0 0 0 0 0 0 0 28 0 11 0 0 0 0.64 0.24 36 R 1 2 -3 -3 4 -2 -3 -3 -3 1 1 -1 0 -2 -3 -2 -1 -3 -2 3 12 20 0 0 12 0 0 0 0 0 13 0 0 0 0 0 0 0 0 43 0.33 0.24 37 A 3 -2 -1 5 -2 1 0 -2 -2 -2 -3 -1 -2 -3 -2 0 -1 -4 -3 0 34 0 0 43 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 13 0.47 0.24 38 V 3 -3 -3 -3 -2 -2 -2 -2 -3 0 2 -2 0 -2 2 -1 -1 -3 -2 3 34 0 0 0 0 0 0 0 0 0 24 0 0 0 13 0 0 0 0 29 0.35 0.24 39 A 3 2 -1 1 -2 -1 1 3 -2 -3 -3 -1 -2 -4 -2 0 -1 -4 -3 -2 45 15 0 9 0 0 9 23 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.24 40 E 1 1 -1 2 -3 0 3 -2 -2 -3 -3 0 -2 -4 -2 1 3 -4 -3 -2 11 9 0 12 0 0 33 0 0 0 0 0 0 0 0 9 27 0 0 0 0.42 0.24 41 V -2 -2 -3 -3 -2 -2 -3 -3 -3 1 4 -2 1 0 -3 -2 -1 -2 -1 1 1 1 1 1 0 1 1 1 0 1 76 1 0 1 1 1 1 0 0 12 0.50 0.20 42 K -1 0 0 4 -3 0 2 -2 3 -3 -3 2 -2 -3 -2 1 -1 -3 -2 -3 1 1 1 30 0 1 14 1 11 1 1 23 0 1 1 11 1 0 0 1 0.41 0.20 43 L -1 -2 -2 -2 -1 -2 -2 -3 -2 1 4 -2 1 0 -2 -2 -1 -2 -1 0 2 1 1 1 1 1 2 2 1 1 76 2 1 1 1 2 2 0 1 2 0.43 0.18 44 P -1 -2 -2 -1 -2 -1 -1 -2 -2 -2 -2 -1 -2 -3 7 -1 -1 -3 -2 -2 2 1 1 1 1 1 2 2 1 1 2 2 1 1 74 2 2 0 1 2 1.46 0.18 45 F -3 -3 -3 -4 -3 -3 -3 -4 4 -1 1 -3 0 7 -4 -3 -3 0 2 -2 0 0 0 0 0 0 0 0 15 0 12 0 0 73 0 0 0 0 0 0 0.88 0.24 46 T 4 -2 -2 -2 -2 -2 -2 -1 -2 -2 -3 -1 -2 -3 5 1 1 -4 -3 -2 49 0 0 0 0 0 0 0 0 0 0 0 0 0 32 9 11 0 0 0 0.73 0.24 47 V -2 -3 -3 2 -2 -3 -2 -4 -3 1 4 -3 1 -1 -3 -3 -2 -3 -2 1 0 0 0 15 0 0 0 0 0 0 74 0 0 0 0 0 0 0 0 11 0.52 0.24 48 P -1 -3 -2 -2 -3 -2 -2 1 -3 -4 -4 -2 -3 -4 7 1 -1 -4 -4 -3 0 0 0 0 0 0 0 15 0 0 0 0 0 0 72 13 0 0 0 0 1.53 0.24 49 R 3 1 -2 -2 -2 -2 -2 3 -2 -3 -3 -1 -2 -3 2 0 1 -3 -3 -2 39 11 0 0 0 0 0 28 0 0 0 0 0 0 12 0 11 0 0 0 0.46 0.24 50 R -2 4 -1 2 -4 3 3 1 -1 -4 -3 0 -2 -4 -2 -1 -2 -3 -3 -3 0 29 0 11 0 21 27 13 0 0 0 0 0 0 0 0 0 0 0 0 0.54 0.24 51 L -2 -3 -4 -4 -2 -3 -4 -4 -4 3 3 -3 1 -1 -3 -3 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 15 45 0 0 0 0 0 0 0 0 40 0.56 0.24 52 L -2 2 -3 -4 -2 -2 -3 -4 -3 2 4 -2 1 2 -4 -3 -2 -2 -1 0 0 15 0 0 0 0 0 0 0 12 64 0 0 9 0 0 0 0 0 0 0.49 0.24 53 L -2 1 -4 -4 -2 -2 -3 -4 -3 1 5 -2 1 0 -4 -3 -2 -3 -2 0 0 12 0 0 0 0 0 0 0 0 88 0 0 0 0 0 0 0 0 0 0.65 0.24 54 H -2 2 0 -2 -4 0 -1 -3 9 -4 -4 -1 -2 -2 -3 -2 -2 -3 1 -4 0 13 0 0 0 0 0 0 87 0 0 0 0 0 0 0 0 0 0 0 1.27 0.24 55 T 0 -2 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 5 3 -4 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 80 20 0 0 0 0.68 0.24 56 E -2 -1 -1 1 -5 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 1.09 0.24 57 G 1 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -3 15 0 0 0 0 0 0 85 0 0 0 0 0 0 0 0 0 0 0 0 1.14 0.24 58 R -2 6 -1 -2 -4 2 -1 -3 -1 -4 -3 1 -2 -4 2 -1 -2 -4 -3 -3 0 75 0 0 0 12 0 0 0 0 0 0 0 0 13 0 0 0 0 0 0.92 0.24 59 E 0 -2 0 4 -3 0 4 1 -2 -4 -4 -1 -3 -4 -2 1 -1 -4 -3 -3 10 0 0 28 0 0 39 12 0 0 0 0 0 0 0 12 0 0 0 0 0.55 0.24 60 D -2 -1 0 5 -4 0 4 -2 5 -4 -4 -1 -3 -4 -2 -1 -2 -4 -2 -4 0 0 0 45 0 0 34 0 21 0 0 0 0 0 0 0 0 0 0 0 0.83 0.24 61 L 1 2 -2 -2 -2 -1 -2 -2 -2 -2 0 -1 -1 2 2 1 2 -3 -1 -1 13 17 0 0 0 0 0 0 0 0 11 0 0 12 10 15 23 0 0 0 0.24 0.24 62 T 1 1 -1 -1 -2 -1 1 -2 -2 -2 -2 -1 -2 -3 2 0 4 -3 -3 -1 12 9 0 0 0 0 12 0 0 0 0 0 0 0 13 0 55 0 0 0 0.49 0.24 63 L -2 -3 -4 -5 -2 -3 -4 -5 -4 1 5 -3 1 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.80 0.24 64 G 1 -1 2 -1 -3 2 2 2 3 -4 -3 1 -2 -4 -2 -1 -2 -3 -2 -3 13 0 11 0 0 15 17 20 12 0 0 12 0 0 0 0 0 0 0 0 0.38 0.24 65 A 2 2 -2 -2 -3 -1 -1 -2 -2 -3 -3 -1 -3 -4 6 1 -1 -4 -3 -2 20 17 0 0 0 0 0 0 0 0 0 0 0 0 51 11 0 0 0 0 1.01 0.24 66 G 0 -3 -1 -2 -3 -3 -3 6 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.40 0.24 67 E -2 -1 -1 1 -5 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 1.09 0.24 68 A 5 -2 -2 -3 -1 -2 -2 0 -2 -2 -2 -1 -2 -3 -2 1 -1 -3 -3 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.75 0.24 69 V 3 -3 -3 -3 -1 -2 -2 -2 -3 1 -1 -2 -1 -2 -2 -1 -1 -4 -2 4 46 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 54 0.47 0.24 70 L -2 -3 -4 -4 -2 -3 -4 -5 -4 2 5 -3 1 0 -4 -3 -2 -3 -2 0 0 0 0 0 0 0 0 0 0 12 88 0 0 0 0 0 0 0 0 0 0.73 0.24 71 V -2 -3 -4 -4 -2 -3 -4 -4 -4 1 5 -3 1 0 -4 -3 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 0 89 0 0 0 0 0 0 0 0 11 0.72 0.24 72 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.32 0.18 K Lambda Standard Ungapped 0.1384 0.3185 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1642 0.3173 PSI Gapped 0.0502 0.2670