Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 R -2 7 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.99 0.13 2 L -2 -2 -4 -4 -2 -2 -3 -4 -3 1 4 -3 4 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 77 0 23 0 0 0 0 0 0 0 0.57 0.13 3 A 3 -1 -1 -2 -2 3 -1 3 -2 -3 -3 -1 -2 -3 -2 0 -1 -3 -2 -2 52 0 0 0 0 23 0 26 0 0 0 0 0 0 0 0 0 0 0 0 0.43 0.13 4 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 5 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 6 D -2 -2 0 5 -4 0 3 -2 0 -3 -3 -1 -3 -1 -2 -1 -2 -1 3 -3 0 0 0 52 0 0 26 0 0 0 0 0 0 0 0 0 0 0 23 0 0.65 0.13 7 G 1 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -2 0 -2 -3 -3 -3 17 0 0 0 0 0 0 83 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.13 8 V -1 2 -3 -3 -2 -2 -2 -4 -3 3 0 -1 0 -1 -3 -2 -1 -3 -2 3 0 23 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0 44 0.35 0.13 9 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 3 -3 1 4 -3 -3 -2 -1 0 2 0 0 0 0 0 0 0 0 0 0 52 0 0 26 0 0 0 0 0 23 0.45 0.13 10 M -1 3 -1 -2 -3 0 -1 -3 5 -1 0 0 4 -2 -3 -1 1 -3 -1 -1 0 26 0 0 0 0 0 0 23 0 0 0 34 0 0 0 17 0 0 0 0.34 0.13 11 Q -1 -1 0 3 -4 3 4 2 -1 -4 -4 0 -2 -4 -2 -1 -1 -3 -3 -3 0 0 0 16 0 18 43 23 0 0 0 0 0 0 0 0 0 0 0 0 0.55 0.13 12 Y -2 3 -1 -2 -3 0 2 -3 4 -1 -2 0 -1 -1 -3 -1 -1 -1 3 1 0 26 0 0 0 0 17 0 16 0 0 0 0 0 0 0 0 0 18 23 0.29 0.13 13 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 14 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 15 E -2 -1 0 5 -4 1 4 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 48 0 0 52 0 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.13 16 A 2 -1 -2 -2 -2 -1 -1 -2 -2 -1 1 1 -1 -2 4 -1 -1 -3 -2 -1 34 0 0 0 0 0 0 0 0 0 23 17 0 0 26 0 0 0 0 0 0.40 0.13 17 D -1 -1 0 3 -4 0 4 3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 18 0 0 48 33 0 0 0 0 0 0 0 0 0 0 0 0 0.62 0.13 18 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 19 L 1 -2 -3 -4 -2 -2 -3 -3 -3 1 4 -2 1 0 -3 -2 -1 -2 -2 0 23 0 0 0 0 0 0 0 0 0 77 0 0 0 0 0 0 0 0 0 0.42 0.13 20 R 2 2 -1 -2 -2 2 -1 4 -1 -3 -3 0 -2 -3 -2 0 -1 -3 -3 -2 26 18 0 0 0 16 0 40 0 0 0 0 0 0 0 0 0 0 0 0 0.45 0.13 21 E -1 3 0 0 -3 1 4 -2 -1 -4 -3 1 -2 -3 -2 2 -1 -3 -2 -3 0 23 0 0 0 0 52 0 0 0 0 0 0 0 0 26 0 0 0 0 0.53 0.13 22 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 23 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.20 0.13 24 P -1 -1 -1 1 -4 1 5 -3 -1 -4 -3 0 -3 -4 3 -1 -1 -4 -3 -3 0 0 0 0 0 0 82 0 0 0 0 0 0 0 18 0 0 0 0 0 0.86 0.13 25 T 2 3 -1 -2 -2 0 -1 -1 -2 -2 -2 0 -2 -3 -2 2 2 -3 -2 -1 33 26 0 0 0 0 0 0 0 0 0 0 0 0 0 23 18 0 0 0 0.34 0.13 26 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -1 -1 -3 -1 5 -4 -2 -2 2 7 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 26 0 0 0 0 74 0 0.99 0.13 27 R -2 6 -1 -2 -3 0 -1 -3 -1 -1 -2 1 -1 -3 -3 -1 -1 -3 -2 1 0 74 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 26 0.60 0.13 28 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 29 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.56 0.13 30 L -1 -3 -4 -4 -2 -3 -3 -4 -3 3 3 -3 1 -1 3 -2 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 23 34 0 0 0 17 0 0 0 0 26 0.43 0.13 31 L -2 -2 -4 -4 -2 -2 -3 -4 -3 1 4 -3 4 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 74 0 26 0 0 0 0 0 0 0 0.56 0.13 32 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.20 0.13 33 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 34 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.16 0.13 35 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 36 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.20 0.13 37 E -1 -1 -1 1 -4 1 5 2 -1 -4 -4 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 77 23 0 0 0 0 0 0 0 0 0 0 0 0 0.71 0.13 38 V 2 -3 -3 -3 -1 -2 -2 -2 -3 2 0 -2 0 -2 -2 -1 0 -3 -2 4 26 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 74 0.39 0.13 39 A 4 -2 -2 -3 -1 -1 -2 -1 -2 -1 -1 -1 4 -2 -2 0 -1 -3 -2 0 74 0 0 0 0 0 0 0 0 0 0 0 26 0 0 0 0 0 0 0 0.42 0.13 40 A 3 3 -1 -2 -2 2 0 -1 -1 -2 -2 0 -1 -3 -2 0 -1 -3 -2 -1 57 26 0 0 0 17 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.13 41 Q -1 0 -1 -2 -3 2 0 -3 0 0 -1 3 -1 0 -2 -1 -1 -1 4 2 0 0 0 0 0 18 0 0 0 0 0 33 0 0 0 0 0 0 23 26 0.28 0.13 42 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.59 0.13 43 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 44 A 3 -2 -2 -1 -2 0 2 -1 -2 -1 1 -1 -1 -2 -2 0 -1 -3 -2 -1 57 0 0 0 0 0 26 0 0 0 17 0 0 0 0 0 0 0 0 0 0.30 0.13 45 W -1 -2 -2 -2 -2 -2 -2 3 -2 -2 -2 -2 -2 0 -2 -1 -2 10 1 -2 2 1 1 1 0 1 1 27 1 1 2 1 1 1 1 2 1 52 1 1 1.15 0.10 46 A 3 -2 -3 -3 -1 -2 -2 -2 -3 0 2 -2 0 -1 -2 0 -1 -3 -2 2 52 0 0 0 0 0 0 0 0 0 26 0 0 0 0 0 0 0 0 23 0.29 0.13 47 M 2 2 -1 -2 -2 0 -1 -1 4 -2 -1 0 3 -2 -2 2 0 -3 -1 -1 23 16 0 0 0 0 0 0 17 0 0 0 18 0 0 26 0 0 0 0 0.25 0.13 48 E -1 3 -1 0 -4 3 4 -3 0 -4 -3 1 -2 -4 -2 -1 -1 -3 -2 -3 0 26 0 0 0 23 52 0 0 0 0 0 0 0 0 0 0 0 0 0 0.66 0.13 49 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.59 0.13 50 P -1 3 -2 -2 -4 -1 -1 -3 -2 -3 -3 0 -3 -4 7 -1 -2 -4 -3 -3 0 26 0 0 0 0 0 0 0 0 0 0 0 0 74 0 0 0 0 0 1.54 0.13 51 N -2 -1 6 1 -3 -1 -1 -1 0 -3 -3 -1 -2 -3 -2 1 2 -4 -2 -2 0 0 74 0 0 0 0 0 0 0 0 0 0 0 0 0 26 0 0 0 0.71 0.13 52 P -2 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -2 -3 -2 7 -2 -2 7 -1 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 74 0 0 26 0 0 1.81 0.13 53 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 54 G -1 -2 3 -1 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -3 0 -2 -3 -3 -4 0 0 23 0 0 0 0 77 0 0 0 0 0 0 0 0 0 0 0 0 0.94 0.13 55 W -2 -3 -3 -4 -3 -2 -3 2 -3 -2 1 -3 -1 0 -4 -2 -2 10 1 -2 0 0 0 0 0 0 0 26 0 0 23 0 0 0 0 0 0 52 0 0 1.18 0.13 56 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.20 0.13 57 S -1 -2 -3 -3 -2 -2 -3 -3 -3 1 4 -2 1 0 -3 1 -1 -2 -2 0 0 0 0 0 0 0 0 0 0 0 82 0 0 0 0 18 0 0 0 0 0.43 0.13 58 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.56 0.13 59 Y -2 -2 -2 -3 -3 -1 -2 -3 5 -2 -2 -2 -2 2 -3 -2 -2 1 7 -2 0 0 0 0 0 0 0 0 23 0 0 0 0 0 0 0 0 0 77 0 0.96 0.13 60 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.59 0.13 61 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 62 F -3 -3 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.03 0.13 63 L -2 -2 -3 -3 -2 3 -1 -3 -2 0 3 -1 1 0 -3 -2 -2 7 0 -1 0 0 0 0 0 26 0 0 0 0 52 0 0 0 0 0 0 23 0 0 0.57 0.13 64 Q -1 0 -1 -1 -3 3 2 4 -1 -4 -3 2 -2 -4 -2 -1 -2 -3 -3 -3 0 0 0 0 0 18 16 43 0 0 0 23 0 0 0 0 0 0 0 0 0.52 0.13 65 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.24 0.13 66 R -1 5 -1 -1 -4 0 2 3 -1 -4 -3 1 -2 -4 -2 -1 -2 -3 -3 -3 0 52 0 0 0 0 23 26 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.13 67 P -2 -3 -3 -3 -2 -2 -2 -3 -3 0 3 -2 0 -1 6 -2 -2 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 48 0 0 0 52 0 0 0 0 0 0.95 0.13 68 I -1 -2 -3 -3 -2 2 -1 -4 -2 2 3 -2 1 -1 -3 -2 -1 -3 -2 2 0 0 0 0 0 23 0 0 0 18 43 0 0 0 0 0 0 0 0 16 0.31 0.13 69 R 2 5 -1 -2 -2 0 -1 -2 -1 -2 -2 1 -2 -3 -2 0 2 -3 -2 -2 26 52 0 0 0 0 0 0 0 0 0 0 0 0 0 0 23 0 0 0 0.45 0.13 70 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 71 L -2 -3 -4 -4 -2 -3 -3 -4 -3 2 4 -3 1 0 -3 -3 -1 -2 -2 2 0 0 0 0 0 0 0 0 0 0 74 0 0 0 0 0 0 0 0 26 0.52 0.13 72 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 73 L -1 -3 -3 -2 -3 -2 -2 -3 -3 -2 0 -2 -1 -3 7 -2 -2 -4 -3 -2 0 0 0 0 0 0 0 0 0 0 18 0 0 0 82 0 0 0 0 0 1.62 0.13 74 G -1 -2 -1 0 -4 0 3 5 -2 -4 -4 -1 -3 -4 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 43 57 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.13 75 K -1 4 -1 -2 -3 0 -1 4 -2 -4 -3 2 -2 -4 -2 -1 -2 -3 -3 -3 0 33 0 0 0 0 0 48 0 0 0 18 0 0 0 0 0 0 0 0 0.62 0.13 76 E -1 3 -1 1 -4 1 5 -3 -1 -4 -3 1 -2 -4 -2 -1 -1 -3 -2 -3 0 26 0 0 0 0 74 0 0 0 0 0 0 0 0 0 0 0 0 0 0.74 0.13 77 V -1 -3 -4 -4 -1 -3 -3 -4 -3 2 3 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 67 0.42 0.09 78 E -1 0 0 2 -4 2 5 -2 0 -3 -3 1 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.02 79 V 0 -3 -3 -3 -1 -2 -3 -3 -3 3 1 -2 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.37 0.02 80 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.43 0.02 K Lambda Standard Ungapped 0.1408 0.3208 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1610 0.3178 PSI Gapped 0.0493 0.2670