Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.48 0.05 2 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 0 -2 -3 -3 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.05 3 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -2 -1 3 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.16 0.13 4 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 5 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.13 6 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.13 7 I -1 -4 -4 -4 7 -3 -4 -4 -4 3 0 -3 0 -2 -3 -2 -1 -3 -2 2 0 0 0 0 43 0 0 0 0 38 0 0 0 0 0 0 0 0 0 19 0.67 0.13 8 A 2 -2 -1 -2 -1 -1 -1 -1 -2 -1 1 -1 -1 -2 -2 4 0 -3 -2 -1 21 0 0 0 0 0 0 0 0 0 23 0 0 0 0 57 0 0 0 0 0.31 0.13 9 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 10 N -2 0 4 0 -3 4 1 -2 4 -4 -3 0 -2 -3 -2 0 -1 -3 -1 -3 0 0 38 0 0 43 0 0 19 0 0 0 0 0 0 0 0 0 0 0 0.62 0.13 11 L -2 -3 -4 -4 -2 -3 -4 -4 -4 4 4 -3 1 0 -3 -3 -1 -3 -2 1 0 0 0 0 0 0 0 0 0 43 57 0 0 0 0 0 0 0 0 0 0.53 0.13 12 E -2 -1 0 5 -4 1 5 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 43 0 0 57 0 0 0 0 0 0 0 0 0 0 0 0 0 0.83 0.13 13 I -1 -3 -2 -3 -2 -2 -2 -3 -3 4 0 -2 0 -1 -2 -1 3 -3 -2 1 0 0 0 0 0 0 0 0 0 57 0 0 0 0 0 0 43 0 0 0 0.39 0.13 14 V -1 -3 -4 -4 -2 -3 -3 -4 -3 4 1 -3 0 3 -3 -3 -1 -2 0 3 0 0 0 0 0 0 0 0 0 38 0 0 0 23 0 0 0 0 0 39 0.44 0.13 15 L -2 -3 -4 -4 -2 -3 -4 -4 -4 4 4 -3 1 0 -3 -3 -1 -3 -2 1 0 0 0 0 0 0 0 0 0 43 57 0 0 0 0 0 0 0 0 0 0.53 0.13 16 K -1 1 -1 0 -4 1 4 -2 -1 -3 -3 4 -2 -4 -2 -1 -1 -4 -2 -3 0 0 0 0 0 0 43 0 0 0 0 57 0 0 0 0 0 0 0 0 0.63 0.13 17 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.13 18 K 2 1 -1 -1 -2 0 0 -1 -1 -3 -3 4 -2 -3 -1 2 0 -3 -2 -2 20 0 0 0 0 0 0 0 0 0 0 57 0 0 0 23 0 0 0 0 0.42 0.13 19 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.60 0.13 20 D -1 0 0 4 -3 0 1 -2 -1 -3 -3 3 -3 -4 -2 2 -1 -4 -3 -3 0 0 0 43 0 0 0 0 0 0 0 36 0 0 0 20 0 0 0 0 0.53 0.13 21 S 0 -1 4 0 -2 0 -1 -1 -1 -3 -3 -1 -2 -3 -2 4 1 -4 -2 -3 0 0 36 0 0 0 0 0 0 0 0 0 0 0 0 64 0 0 0 0 0.57 0.13 22 P -1 -2 -1 -1 -3 0 3 -3 -2 -1 -2 -1 -1 -3 4 -1 2 -3 -2 1 0 0 0 0 0 0 36 0 0 0 0 0 0 0 21 0 20 0 0 23 0.42 0.13 23 E -1 0 -1 1 -4 1 5 -2 -1 -4 -3 3 -2 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 77 0 0 0 0 23 0 0 0 0 0 0 0 0 0.75 0.13 24 V -1 -2 -2 -3 -2 -2 -2 -3 -1 2 0 -2 0 0 -3 1 0 -1 3 3 0 0 0 0 0 0 0 0 0 18 0 0 0 0 0 23 0 0 20 39 0.27 0.13 25 S 1 -1 0 -1 -1 0 -1 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.64 0.13 26 E -1 3 -1 -1 -3 3 2 -3 -1 -1 -2 3 -1 -3 -2 -1 -1 -3 -2 1 0 20 0 0 0 19 21 0 0 0 0 23 0 0 0 0 0 0 0 18 0.36 0.13 27 T -1 -1 5 0 -2 -1 -1 2 -1 -3 -3 -1 -2 -3 -2 2 2 -4 -3 -2 0 0 43 0 0 0 0 18 0 0 0 0 0 0 0 19 21 0 0 0 0.48 0.13 28 L -2 0 -2 -2 -2 -1 -1 -3 -2 2 3 3 0 -1 -2 -2 -1 -3 -2 0 0 0 0 0 0 0 0 0 0 18 39 43 0 0 0 0 0 0 0 0 0.28 0.13 29 V 4 -2 -3 -3 -1 -2 -2 -1 -3 0 -1 -2 -1 -2 -2 0 0 -3 -2 3 62 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 38 0.37 0.13 30 T -1 -1 -1 1 -3 1 5 -3 -1 -3 -3 0 -2 -4 -2 0 2 -3 -2 -2 0 0 0 0 0 0 79 0 0 0 0 0 0 0 0 0 21 0 0 0 0.70 0.13 31 R -2 5 -2 -3 -3 0 -1 -3 -1 -1 1 1 0 3 -3 -2 -2 -2 0 -2 0 57 0 0 0 0 0 0 0 0 20 0 0 23 0 0 0 0 0 0 0.46 0.13 32 I -2 -3 -4 -4 -2 -3 -4 -4 -4 4 4 -3 1 0 -3 -3 -1 -3 -2 1 0 0 0 0 0 0 0 0 0 41 59 0 0 0 0 0 0 0 0 0 0.53 0.13 33 C -1 -3 -4 -4 8 -3 -4 -4 -3 0 3 -3 0 -1 -3 -2 -1 -3 -2 0 0 0 0 0 57 0 0 0 0 0 43 0 0 0 0 0 0 0 0 0 0.85 0.13 34 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 35 N -1 1 3 -1 -4 1 0 -2 -1 -3 -3 5 -2 -4 -2 0 -1 -4 -2 -3 0 0 21 0 0 0 0 0 0 0 0 79 0 0 0 0 0 0 0 0 0.61 0.13 36 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 37 L -2 -1 -2 -3 -2 3 -1 -3 -2 0 3 -1 5 -1 -3 -2 -1 -2 -2 0 0 0 0 0 0 23 0 0 0 0 38 0 39 0 0 0 0 0 0 0 0.39 0.13 38 L -1 -2 -2 -1 -2 0 3 -3 -2 1 1 -1 0 -2 -2 -1 -1 -3 -2 2 0 0 0 0 0 0 43 0 0 0 21 0 0 0 0 0 0 0 0 36 0.30 0.13 39 S 4 -2 -1 -2 -1 -1 -1 0 -2 -2 -2 -1 -2 -3 -1 2 0 -3 -2 -1 79 0 0 0 0 0 0 0 0 0 0 0 0 0 0 21 0 0 0 0 0.51 0.13 40 K -2 4 -1 -2 -3 0 -1 -3 0 -3 -2 2 -2 1 -3 -1 -2 0 5 -2 0 36 0 0 0 0 0 0 0 0 0 21 0 0 0 0 0 0 43 0 0.58 0.13 41 E -1 1 -1 0 -4 1 5 -2 -1 -4 -3 4 -2 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 0 57 0 0 0 0 43 0 0 0 0 0 0 0 0 0.66 0.13 42 V -1 -3 -4 -4 -1 -3 -3 -4 -4 3 1 -3 0 -1 -3 -2 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 23 0 0 0 0 0 0 0 0 0 77 0.53 0.13 43 S 0 -2 -1 -2 5 -2 -2 -2 -3 1 -1 -2 -1 -2 -2 3 0 -3 -2 3 0 0 0 0 19 0 0 0 0 0 0 0 0 0 0 38 0 0 0 43 0.33 0.13 44 F -3 -3 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.03 0.13 45 L 1 -3 -3 -4 -1 -3 -3 -3 -3 3 3 -3 1 -1 -3 -2 -1 -3 -2 2 18 0 0 0 0 0 0 0 0 23 39 0 0 0 0 0 0 0 0 20 0.33 0.13 46 K -1 1 3 0 -3 4 1 -2 0 -3 -3 4 -2 -4 -2 0 -1 -3 -2 -3 0 0 23 0 0 36 0 0 0 0 0 41 0 0 0 0 0 0 0 0 0.55 0.13 47 A 4 -2 -3 -3 -1 -2 -2 -1 -3 2 0 -2 0 -2 -2 0 -1 -3 -2 2 57 0 0 0 0 0 0 0 0 23 0 0 0 0 0 0 0 0 0 20 0.33 0.13 48 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.16 0.13 49 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.25 0.13 50 S 0 -1 0 0 -2 0 4 -2 -1 -2 -3 0 -2 -3 -1 2 3 -3 -2 -2 0 0 0 0 0 0 43 0 0 0 0 0 0 0 0 21 36 0 0 0 0.47 0.13 51 V 2 -2 -2 -3 -1 -2 -2 -2 -3 1 0 -2 0 -2 -2 0 2 -3 -2 4 23 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 20 0 0 57 0.32 0.13 52 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 53 N -1 1 3 1 -4 1 4 -2 -1 -4 -3 3 -2 -4 -2 0 -1 -4 -2 -3 0 0 21 0 0 0 43 0 0 0 0 36 0 0 0 0 0 0 0 0 0.55 0.13 54 F -3 -3 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.03 0.13 55 K -1 1 0 3 -4 1 3 -2 -1 -3 -3 4 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 23 0 0 20 0 0 0 0 57 0 0 0 0 0 0 0 0 0.57 0.13 56 L -2 -3 -4 -4 -2 -3 -4 -4 -4 4 4 -3 1 0 -3 -3 -1 -3 -2 1 0 0 0 0 0 0 0 0 0 43 57 0 0 0 0 0 0 0 0 0 0.53 0.13 57 S -1 -2 2 -2 8 -2 -2 -2 -2 -2 -2 -2 -2 -3 -3 2 0 -3 -3 -2 0 0 19 0 61 0 0 0 0 0 0 0 0 0 0 21 0 0 0 0 0.85 0.13 58 D -2 -2 5 5 -4 -1 1 -1 -1 -4 -4 -1 -3 -4 -2 0 -1 -5 -3 -4 0 0 43 57 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.86 0.13 59 M -2 -3 -4 -4 -2 -2 -3 -4 -3 3 3 -3 4 0 -3 -3 -1 -2 -2 1 0 0 0 0 0 0 0 0 0 36 43 0 21 0 0 0 0 0 0 0 0.48 0.13 60 E -1 -1 0 3 -4 1 5 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 19 0 0 81 0 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.13 61 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -2 -1 3 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.16 0.13 62 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 63 I -1 -3 -4 -4 -1 -3 -3 -4 -4 4 1 -3 1 -1 -3 -3 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 57 0 0 0 0 0 0 0 0 0 43 0.53 0.13 64 T 0 -1 0 0 -2 0 4 -2 -1 -2 -3 0 -2 -3 -2 2 3 -3 -2 -2 0 0 0 0 0 0 43 0 0 0 0 0 0 0 0 19 38 0 0 0 0.47 0.13 65 N -2 -1 6 3 -3 0 0 -1 0 -4 -4 -1 -3 -4 -2 0 -1 -4 -3 -4 0 0 77 23 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.13 66 T 0 -2 0 -2 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.69 0.13 67 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 68 E -1 -1 -1 1 -4 2 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 69 L -2 -3 -4 -4 -2 -3 -3 -4 -3 3 2 -3 1 0 -4 -3 -2 9 0 0 0 0 0 0 0 0 0 0 0 31 31 0 0 0 0 0 0 38 0 0 0.87 0.11 70 P -1 -2 -2 -1 -3 -1 -1 -2 -2 -3 -3 -1 -2 -4 7 -1 -1 -4 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 K Lambda Standard Ungapped 0.1294 0.3092 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1574 0.3180 PSI Gapped 0.0482 0.2670