Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -1 -2 -3 -4 -2 -1 -2 -3 -2 1 2 -2 7 0 -3 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.60 0.11 2 P -1 -3 -2 -2 -3 -2 -1 -3 -3 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.17 0.11 3 A 2 -2 -1 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -3 -1 1 4 -3 -2 0 34 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 66 0 0 0 0.46 0.11 4 Y -2 -2 -3 -4 -3 -2 -2 -4 1 -2 -1 -2 -1 3 -3 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.10 0.11 5 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.52 0.11 6 F -3 -3 -3 -4 -3 -4 -4 -4 -2 0 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.97 0.11 7 S -1 -1 4 4 -3 0 0 -1 -1 -3 -4 -1 -3 -3 -2 2 0 -4 -3 -3 0 0 33 33 0 0 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0.56 0.11 8 K -1 1 -1 0 -4 1 3 -2 -1 -3 -3 5 -2 -4 -1 -1 -1 -3 -2 -3 0 0 0 0 0 0 33 0 0 0 0 67 0 0 0 0 0 0 0 0 0.60 0.11 9 E -1 -1 4 1 -4 1 5 -2 0 -4 -3 0 -2 -4 -2 0 -1 -4 -2 -3 0 0 33 0 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0.69 0.11 10 S 1 -2 0 -1 -2 -1 -1 3 -2 -3 -3 -1 -2 -3 -2 4 0 -3 -2 -2 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 67 0 0 0 0 0.55 0.11 11 F -3 -3 -3 -4 -3 -4 -4 -4 -2 0 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.97 0.11 12 L -2 -2 -3 -4 -2 -2 -3 -4 -3 1 4 -2 5 0 -3 -3 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 67 0 33 0 0 0 0 0 0 0 0.52 0.11 13 K -1 1 0 4 -4 1 1 -2 -1 -3 -3 4 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 33 0 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0.58 0.11 14 F -3 -3 -3 -4 -3 -4 -4 -4 -2 0 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.97 0.11 15 L -2 -3 -4 -4 -2 -3 -3 -4 -3 3 4 -3 2 0 -3 -3 -1 -2 -2 1 0 0 0 0 0 0 0 0 0 33 67 0 0 0 0 0 0 0 0 0 0.51 0.11 16 E -1 1 -1 1 -4 1 5 -2 -1 -3 -3 3 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 67 0 0 0 0 33 0 0 0 0 0 0 0 0 0.67 0.11 17 G -1 1 -1 -1 -3 0 0 3 -1 -3 -3 4 -2 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 0 34 0 0 0 66 0 0 0 0 0 0 0 0 0.55 0.11 18 H -2 -1 5 0 -3 0 -1 -1 6 -4 -4 -1 -2 -3 -2 0 -1 -4 -1 -3 0 0 66 0 0 0 0 0 34 0 0 0 0 0 0 0 0 0 0 0 0.76 0.11 19 L -1 -3 -4 -4 -1 -3 -3 -4 -3 2 4 -3 1 0 -3 -3 -1 -2 -1 3 0 0 0 0 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 33 0.47 0.11 20 E -1 -1 -1 1 -4 1 5 3 -1 -4 -4 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 67 33 0 0 0 0 0 0 0 0 0 0 0 0 0.65 0.11 21 D -1 -2 0 4 -4 0 3 3 -1 -4 -4 -1 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 34 0 0 33 33 0 0 0 0 0 0 0 0 0 0 0 0 0.61 0.11 22 D -2 0 1 5 -4 0 1 -2 -1 -3 -4 3 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 67 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0.74 0.11 23 V 0 -2 -2 -3 -1 -2 -2 -3 -3 2 0 -2 0 -2 -2 -1 3 -3 -2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 33 0 0 67 0.37 0.11 24 V 2 -2 -3 -3 -1 -1 -2 -2 -2 1 1 -2 4 -1 -2 -1 -1 -3 -2 3 33 0 0 0 0 0 0 0 0 0 0 0 33 0 0 0 0 0 0 34 0.28 0.11 25 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.52 0.11 26 V -1 -3 -3 -4 -1 -3 -3 -4 -4 4 1 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0 67 0.49 0.11 27 V 0 -2 -2 -2 -1 -1 -2 -2 -2 2 0 -1 0 -1 -1 -1 0 -2 -1 4 3 2 1 2 1 1 2 2 1 2 3 2 1 1 2 2 2 0 1 69 0.23 0.07 28 S 1 -1 0 -1 -1 0 0 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.60 0.11 29 S 1 -1 0 -1 -1 0 0 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.60 0.11 30 D -2 -2 1 7 -4 -1 1 -2 -1 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.11 31 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.52 0.11 32 T -1 -2 -1 -2 -1 -1 -2 -3 -2 0 2 -2 0 -1 -2 0 4 -3 -2 0 0 0 0 0 0 0 0 0 0 0 33 0 0 0 0 0 67 0 0 0 0.39 0.11 33 D -2 -2 1 7 -4 -1 1 -2 -1 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.11 34 F -1 -3 -4 -4 -2 -3 -3 -4 -3 3 1 -3 0 4 -3 -3 -1 -1 1 3 0 0 0 0 0 0 0 0 0 33 0 0 0 34 0 0 0 0 0 33 0.43 0.11 35 C -2 -3 0 5 6 -1 0 -2 -2 -3 -3 -2 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 66 34 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.11 36 K -1 0 -1 0 -3 0 3 -3 -1 2 -1 3 -1 -2 -2 -1 -1 -3 -2 0 0 0 0 0 0 0 33 0 0 33 0 34 0 0 0 0 0 0 0 0 0.28 0.11 37 K 2 0 -1 0 -2 1 3 -1 -1 -3 -3 3 -2 -3 -1 0 -1 -3 -2 -2 33 0 0 0 0 0 33 0 0 0 0 34 0 0 0 0 0 0 0 0 0.39 0.11 38 L -1 -1 -1 0 -2 0 3 -2 -1 -1 2 -1 0 -2 -2 2 0 -3 -2 -1 0 0 0 0 0 0 33 0 0 0 34 0 0 0 0 33 0 0 0 0 0.24 0.11 39 S 0 -2 -1 -2 -2 -1 -2 3 -2 -1 -1 -1 4 -2 -2 2 0 -3 -2 -1 0 0 0 0 0 0 0 33 0 0 0 0 33 0 0 34 0 0 0 0 0.34 0.11 40 E -1 -1 4 1 -4 1 5 -2 0 -4 -3 0 -2 -4 -2 0 -1 -4 -2 -3 0 0 33 0 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0.69 0.11 41 S 1 -1 0 -1 -1 -1 -1 -1 -1 -2 -2 -1 -2 -3 -1 4 3 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 67 33 0 0 0 0.50 0.11 42 M -2 -1 -1 -3 -3 -1 -2 -3 6 -1 0 -2 4 1 -3 -2 -2 0 5 -1 0 0 0 0 0 0 0 0 33 0 0 0 34 0 0 0 0 0 33 0 0.52 0.11 43 V -1 -3 -4 -4 -1 -3 -3 -4 -3 2 4 -3 1 0 -3 -3 -1 -2 -1 3 0 0 0 0 0 0 0 0 0 0 66 0 0 0 0 0 0 0 0 34 0.47 0.11 44 G 0 -3 -1 -2 -3 -2 -3 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.21 0.11 45 E -1 0 -1 0 -3 1 3 -3 -1 0 -1 3 -1 -3 -2 -1 -1 -3 -2 2 0 0 0 0 0 0 34 0 0 0 0 33 0 0 0 0 0 0 0 33 0.29 0.11 46 K -1 4 -1 -1 -4 1 0 -2 -1 -3 -3 5 -2 -3 -2 -1 -1 -3 -2 -3 0 33 0 0 0 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0.66 0.11 47 E -1 0 0 4 -4 1 3 -2 -1 -3 -3 3 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 33 0 0 34 0 0 0 0 33 0 0 0 0 0 0 0 0 0.59 0.11 48 Y -2 -2 -1 -3 -3 -1 -2 -3 6 -2 -1 -2 -1 4 -3 -2 -2 1 5 -2 0 0 0 0 0 0 0 0 33 0 0 0 0 33 0 0 0 0 34 0 0.71 0.11 49 C -2 -3 -3 -4 6 -3 -3 -3 -1 -1 -1 -3 -1 4 -4 -2 -2 1 5 -1 0 0 0 0 34 0 0 0 0 0 0 0 0 33 0 0 0 0 33 0 0.74 0.11 50 F -1 -3 -3 -4 -2 -2 -3 -3 -2 1 1 -3 4 4 -3 -2 -1 -1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 33 34 0 0 0 0 0 33 0.39 0.11 51 A 2 -3 -3 -3 -1 -2 -2 -2 -3 2 0 -2 0 -2 -2 -1 0 -3 -2 4 34 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 66 0.34 0.11 52 E -1 0 0 0 -3 1 3 -2 -1 -2 -3 3 -2 -3 -1 0 3 -3 -2 -2 0 0 0 0 0 0 34 0 0 0 0 33 0 0 0 0 33 0 0 0 0.42 0.11 53 F -2 -3 -4 -4 -2 -4 -4 -4 -2 3 1 -3 0 6 -4 -3 -2 0 2 0 0 0 0 0 0 0 0 0 0 33 0 0 0 67 0 0 0 0 0 0 0.63 0.11 54 A 4 -2 -1 -2 -1 -1 -2 3 -2 -2 -2 -1 -2 -3 -2 0 -1 -3 -3 -1 67 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0 0 0 0 0.54 0.11 55 F -1 -3 -4 -4 -2 -3 -3 -4 -3 3 1 -3 0 4 -3 -3 -1 -1 1 3 0 0 0 0 0 0 0 0 0 33 0 0 0 34 0 0 0 0 0 33 0.43 0.11 56 P 0 -2 -1 -2 -2 -1 -1 -2 -2 0 -1 -1 -1 -2 5 2 0 -4 -2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 34 33 0 0 0 33 0.54 0.11 57 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.11 58 D -2 -2 1 7 -4 -1 1 -2 -1 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.11 59 I -1 -3 -3 -4 -1 -3 -3 -4 -4 4 1 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0 0 0 0 0 66 0.49 0.11 60 F -3 -3 -3 -4 -3 -4 -4 -4 -2 0 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.97 0.11 61 D -2 -1 0 4 -4 1 5 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 34 0 0 66 0 0 0 0 0 0 0 0 0 0 0 0 0 0.79 0.11 62 A 2 0 -2 -2 -2 -1 -1 -2 -2 0 -1 3 -1 -2 -2 0 -1 -3 -2 2 34 0 0 0 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 33 0.22 0.11 63 D -2 -2 1 7 -4 -1 1 -2 -1 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.11 64 E -1 -2 -2 -1 -2 0 3 -3 -2 1 2 -1 0 -1 -2 -2 -1 -3 -2 2 0 0 0 0 0 0 34 0 0 0 33 0 0 0 0 0 0 0 0 33 0.25 0.11 65 D -2 -2 1 7 -4 -1 1 -2 -1 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.11 66 E -1 1 -1 1 -4 1 5 -2 -1 -3 -3 3 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 67 0 0 0 0 33 0 0 0 0 0 0 0 0 0.67 0.11 67 I -2 -3 -4 -4 -2 -4 -4 -4 -2 3 1 -3 0 6 -4 -3 -2 0 2 1 0 0 0 0 0 0 0 0 0 34 0 0 0 66 0 0 0 0 0 0 0.63 0.11 68 D -2 -2 1 7 -4 -1 1 -2 -1 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.11 69 E -1 0 -1 1 -4 2 6 -3 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.11 70 M -1 0 -2 -2 -2 -1 -1 -3 -2 3 1 3 4 -1 -2 -2 -1 -3 -2 1 0 0 0 0 0 0 0 0 0 33 0 33 34 0 0 0 0 0 0 0 0.26 0.11 71 M -1 -2 -3 -3 -2 -2 -2 -3 -3 0 2 -2 4 -1 5 -2 -1 -3 -2 0 0 0 0 0 0 0 0 0 0 0 33 0 34 0 33 0 0 0 0 0 0.62 0.11 72 K -1 2 0 -1 -4 1 1 -2 -1 -3 -3 5 -2 -4 -1 -1 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.69 0.11 73 Y -2 -2 -3 -4 -3 -2 -2 -4 1 -2 -1 -2 -1 3 -3 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.10 0.11 74 A 2 -3 -3 -3 6 -2 -3 -2 -3 0 2 -2 0 -1 -2 -1 -1 -3 -2 0 34 0 0 0 33 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0 0.44 0.11 75 I -1 -3 -3 -4 -1 -3 -3 -4 -4 4 1 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0 0 0 0 0 66 0.49 0.11 76 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.52 0.11 77 F -3 -3 -3 -4 -3 -4 -4 -4 -2 0 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.97 0.11 78 V 0 -2 -2 -3 -1 -2 -2 -3 -3 2 0 -2 0 -2 -2 -1 3 -3 -2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 33 0 0 67 0.37 0.11 79 E 0 -1 0 1 -3 1 5 -2 -1 -3 -3 0 -2 -3 -1 2 0 -3 -2 -3 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 33 0 0 0 0 0.60 0.11 80 K 0 0 0 -1 -2 0 0 -1 -1 -3 -3 3 -2 -3 -1 4 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 34 0 0 0 66 0 0 0 0 0.45 0.11 81 E -2 -1 1 6 -4 0 4 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 66 0 0 34 0 0 0 0 0 0 0 0 0 0 0 0 0 0.87 0.11 82 K -1 1 -1 1 -4 1 5 -2 -1 -3 -3 3 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 66 0 0 0 0 34 0 0 0 0 0 0 0 0 0.67 0.11 83 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.61 0.11 84 S 1 -1 0 -1 -1 -1 -1 -1 -1 -2 -2 -1 -2 -3 -1 4 3 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 67 33 0 0 0 0.50 0.11 85 E -1 0 0 1 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.82 0.06 86 A 3 -2 -3 -3 -1 -2 -2 -1 -3 3 0 -2 0 -1 -2 0 -1 -3 -2 1 50 0 0 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 0 0 0.25 0.06 87 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 0 -2 -3 -3 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.14 0.06 88 R -1 5 -1 -1 -4 1 0 -2 -1 -3 -3 4 -2 -3 -2 -1 -1 -3 -2 -3 0 50 0 0 0 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 0.63 0.06 89 N -1 1 5 0 -3 1 0 -1 0 -3 -3 4 -2 -3 -2 0 -1 -4 -2 -3 0 0 50 0 0 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 0.52 0.06 90 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.49 0.06 91 I -1 -2 -3 -3 -2 -2 -3 -4 -3 3 2 -2 5 0 -3 -2 -1 -2 -1 2 0 0 0 0 0 0 0 0 0 50 0 0 50 0 0 0 0 0 0 0 0.38 0.06 92 R -2 6 -1 -2 -4 1 0 -3 0 -3 -2 2 -2 -3 -2 -1 -1 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.86 0.06 K Lambda Standard Ungapped 0.1363 0.3200 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1541 0.3178 PSI Gapped 0.0472 0.2670