Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 D -2 -2 4 5 -4 0 3 -2 -1 -4 -4 -1 -3 -4 -2 -1 -1 -4 -3 -4 0 0 26 56 0 0 18 0 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.19 2 W -3 -3 -4 -5 -3 -3 -4 -3 -3 -3 -2 -4 -2 0 -4 -3 -3 11 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.79 0.20 3 K -1 1 -1 -1 -3 0 0 -2 -2 -3 -3 5 -2 -3 -2 0 3 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 67 0 0 0 0 33 0 0 0 0.58 0.20 4 Q -1 0 -1 -1 -3 6 1 -3 -1 -2 0 0 -1 -3 1 -1 -1 -3 -2 -2 0 0 0 0 0 79 0 0 0 0 15 0 0 0 7 0 0 0 0 0 0.70 0.20 5 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -4 -2 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.28 0.20 6 E -1 -1 -2 0 -3 1 5 -3 -1 0 -2 0 -1 -3 -2 -1 -1 -4 -2 2 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 33 0.56 0.20 7 L -2 -3 -4 -4 -2 -2 -3 -4 -3 2 4 -3 4 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 9 67 0 24 0 0 0 0 0 0 0 0.61 0.20 8 E -1 0 -1 0 -4 1 5 -3 -1 -4 -3 3 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 67 0 0 0 0 33 0 0 0 0 0 0 0 0 0.77 0.20 9 S 0 -1 0 0 -2 0 3 -1 -1 -3 -3 0 -2 -3 -2 4 0 -3 -2 -3 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 67 0 0 0 0 0.59 0.20 10 D -2 -2 4 5 -4 -1 0 -2 -1 -4 -4 -1 -3 -4 -2 1 -1 -5 -3 -4 0 0 33 60 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0.89 0.20 11 E 0 -2 -1 1 -3 -1 2 5 -2 -4 -4 -1 -3 -4 -2 2 -1 -3 -3 -3 0 0 0 8 0 0 18 52 0 0 0 0 0 0 0 21 0 0 0 0 0.64 0.20 12 H -1 -2 1 3 -3 -1 -1 3 4 -3 -3 -2 -3 -3 -2 0 2 -3 -2 -3 0 0 7 20 0 0 0 33 18 0 0 0 0 0 0 0 21 0 0 0 0.49 0.20 13 G -1 -2 -1 -1 -4 -1 3 5 -2 -4 -4 -1 -3 -4 -2 -1 -2 -3 -3 -4 0 0 0 0 0 0 33 67 0 0 0 0 0 0 0 0 0 0 0 0 0.86 0.20 14 K -1 3 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 9 0 0 0 0 0 0 0 0 0 91 0 0 0 0 0 0 0 0 0.81 0.20 15 T 0 -2 0 -1 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 -2 4 4 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 55 45 0 0 0 0.60 0.20 16 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.75 0.20 17 R -1 4 -1 -2 -2 0 -1 -2 -2 -2 0 1 -1 -3 -2 3 2 -3 -2 -2 0 32 0 0 0 0 0 0 0 0 14 8 0 0 0 33 13 0 0 0 0.34 0.20 18 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.75 0.20 19 T 0 -2 -2 -3 -2 -2 -2 -3 -2 -1 -1 -2 -1 5 -3 0 4 -1 1 -1 6 0 0 0 0 0 0 0 0 0 0 0 0 42 0 0 52 0 0 0 0.53 0.20 20 L 0 -3 -3 -3 -2 -2 -3 -3 -3 3 2 -2 2 -1 5 -2 -1 -3 -2 1 7 0 0 0 0 0 0 0 0 27 21 0 7 0 31 0 0 0 0 7 0.56 0.21 21 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -3 -4 -2 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.30 0.21 22 E -1 -2 -1 3 -3 0 3 1 -2 -1 -2 -1 -2 -3 -2 -1 -1 -4 -3 2 0 0 0 21 0 0 35 12 0 0 0 0 0 0 0 0 0 0 0 31 0.37 0.21 23 G -1 -3 1 -2 -3 -2 -3 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 8 0 0 0 0 92 0 0 0 0 0 0 0 0 0 0 0 0 1.22 0.21 24 L -2 -3 -4 -4 -2 -3 -4 -4 -4 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.77 0.21 25 S 0 -2 0 -1 -2 -1 -1 -2 -2 -2 -2 -1 -2 -3 -2 3 5 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 29 71 0 0 0 0.66 0.21 26 G -1 0 1 -1 -3 1 -1 3 -2 -3 -3 3 -2 -4 1 0 2 -3 -3 -3 0 0 7 0 0 8 0 27 0 0 0 31 0 0 7 0 19 0 0 0 0.40 0.21 27 E -1 -1 -2 0 -3 1 5 -3 -1 1 -2 0 -1 -3 -2 -1 -1 -4 -2 2 0 0 0 0 0 0 69 0 0 8 0 0 0 0 0 0 0 0 0 23 0.57 0.21 28 Q -1 0 -1 -1 -4 7 1 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.04 0.21 29 K -1 4 -1 -2 -3 2 0 -2 -1 -3 -3 4 -2 -3 -2 0 3 -3 -2 -2 0 26 0 0 0 7 0 0 0 0 0 36 0 0 0 0 31 0 0 0 0.52 0.21 30 S 0 -1 0 1 -2 0 3 -1 -1 -3 -3 -1 -2 -3 -2 4 2 -4 -3 -2 0 0 0 7 0 0 24 0 0 0 0 0 0 0 0 56 13 0 0 0 0.51 0.21 31 Q -1 0 -1 -1 -4 7 1 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.04 0.21 32 W -3 -3 -4 -5 -3 -3 -4 -3 -3 -3 -2 -4 -2 0 -4 -3 -3 12 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.83 0.21 33 M -2 2 2 -2 -3 -1 -2 -3 -2 1 2 2 2 -2 -3 -1 1 -3 -2 -1 0 15 13 0 0 0 0 0 0 8 29 16 6 0 0 0 12 0 0 0 0.21 0.21 34 L 2 0 -1 -2 -2 0 -1 -1 -2 -2 0 3 -1 -3 -2 3 0 -3 -2 -2 28 0 0 0 0 0 0 0 0 0 11 31 0 0 0 30 0 0 0 0 0.31 0.21 35 T -1 -2 -1 -2 -2 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.83 0.21 36 I -2 -4 -4 -4 -2 -3 -4 -4 -4 6 1 -3 1 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.77 0.21 37 K -1 1 -1 0 -4 1 4 -2 -1 -4 -3 4 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 52 0 0 0 0 48 0 0 0 0 0 0 0 0 0.73 0.21 38 A 5 -2 -2 -2 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 -1 1 1 -3 -2 -1 92 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 0.65 0.21 39 V -1 -3 -4 -4 -2 -3 -3 -4 -4 2 3 -3 1 -1 -3 -3 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0 0 0 0 66 0.56 0.21 40 V -1 -3 -4 -4 6 -3 -3 -4 -4 1 0 -3 0 -2 -3 -2 -1 -3 -2 4 0 0 0 0 31 0 0 0 0 0 0 0 0 0 0 0 0 0 0 69 0.71 0.21 41 Q -1 0 -1 0 -4 6 4 -3 0 -4 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 69 31 0 0 0 0 0 0 0 0 0 0 0 0 0 0.88 0.21 42 S 1 -1 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.75 0.21 43 A 4 -2 -1 -2 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 -1 3 0 -3 -2 -1 69 0 0 0 0 0 0 0 0 0 0 0 0 0 0 31 0 0 0 0 0.58 0.21 44 K -1 3 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 8 0 0 0 0 0 0 0 0 0 92 0 0 0 0 0 0 0 0 0.83 0.21 45 H -2 -2 -2 -3 -3 -2 -2 -4 4 -2 -2 -2 -2 2 -4 -2 -2 1 8 -2 0 0 0 0 0 0 0 0 11 0 0 0 0 0 0 0 0 0 89 0 1.20 0.21 46 W -3 -3 -4 -5 -3 -3 -4 -3 -3 -3 -2 -4 -2 0 -4 -3 -3 12 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.83 0.21 47 N -2 -1 7 1 -3 -1 -1 -1 0 -4 -4 -1 -3 -4 -3 0 -1 -4 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.13 0.21 48 L -2 -3 -4 -4 -2 -3 -4 -4 -4 4 4 -3 1 0 -4 -3 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 48 52 0 0 0 0 0 0 0 0 0 0.62 0.21 49 A 4 -2 -1 -2 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 -1 3 0 -3 -2 -1 63 0 0 0 0 0 0 0 0 0 0 0 0 0 0 37 0 0 0 0 0.57 0.21 50 E -1 -1 -1 1 -4 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 1.06 0.21 51 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.80 0.21 52 T 0 1 0 -1 -2 3 0 -2 -1 -2 -3 0 -2 -3 -2 3 3 -3 -2 -2 0 7 0 0 0 24 0 0 0 0 0 0 0 0 0 34 35 0 0 0 0.47 0.21 53 F -2 -3 -4 -4 -2 -3 -4 -4 -3 3 1 -3 0 4 -4 -3 -1 -2 0 3 0 0 0 0 0 0 0 0 0 31 7 0 0 27 0 0 0 0 0 34 0.51 0.21 54 E -1 -1 -1 0 -3 1 5 -3 -1 -1 0 0 1 -3 -2 -1 -1 -3 -2 1 0 0 0 0 0 0 69 0 0 0 8 0 7 0 0 0 0 0 0 15 0.55 0.21 55 A 2 -1 2 2 -2 -1 0 -1 -1 -3 -3 2 -2 -3 -2 2 2 -4 -3 -2 21 0 16 13 0 0 0 0 0 0 0 21 0 0 0 13 15 0 0 0 0.33 0.21 56 S 0 -2 1 -1 -2 -1 -1 -2 -2 0 0 -1 1 -2 -2 3 3 -3 1 -1 0 0 7 0 0 0 0 0 0 7 8 0 6 0 0 44 22 0 6 0 0.30 0.21 57 G -1 -1 0 4 -4 0 2 3 -2 -4 -4 0 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 32 0 0 21 31 0 0 0 7 0 0 0 8 0 0 0 0 0.58 0.21 58 E -2 1 2 3 -4 3 2 -2 3 -4 -3 0 -2 -4 -2 0 -1 -4 -2 -3 0 7 8 23 0 26 16 0 12 0 0 0 0 0 0 7 0 0 0 0 0.52 0.21 59 G -1 -3 -1 -2 5 -2 -1 5 -3 -3 -3 -2 1 -3 -3 0 -2 -3 -3 -3 0 0 0 0 23 0 6 57 0 0 0 0 6 0 0 8 0 0 0 0 0.73 0.21 60 V -2 -3 -4 -4 -2 -3 -4 -4 -4 4 3 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 34 31 0 0 0 0 0 0 0 0 35 0.54 0.21 61 I -1 -3 -3 -3 -2 -3 -3 -4 -3 4 1 -3 2 -1 -3 -1 2 -3 -2 2 0 0 0 0 0 0 0 0 0 49 6 0 7 0 0 0 24 0 0 14 0.43 0.21 62 I -2 -3 -4 -4 -2 -2 -3 -4 -3 4 3 -3 4 0 -3 -3 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 39 37 0 24 0 0 0 0 0 0 0 0.56 0.21 63 K -2 4 -1 -2 -4 1 0 -2 -1 -4 -3 5 -2 -4 -2 -1 -1 -4 -2 -3 0 34 0 0 0 0 0 0 0 0 0 66 0 0 0 0 0 0 0 0 0.80 0.21 64 K -1 -1 -1 0 -4 0 4 3 -1 -4 -4 1 -3 -4 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 55 33 0 0 0 13 0 0 0 0 0 0 0 0 0.65 0.21 65 R -2 7 -1 -2 -4 0 -1 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.21 K Lambda Standard Ungapped 0.1281 0.3114 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1525 0.3140 PSI Gapped 0.0467 0.2670