Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 P -1 -2 -2 -2 -3 -2 -1 -2 -3 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.18 0.11 2 E -1 0 0 1 -4 4 5 -2 0 -3 -3 1 -2 -4 -2 0 -1 -3 -2 -3 0 0 0 0 0 32 68 0 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.11 3 A 4 -2 -2 -2 -1 -1 -2 -1 -2 -1 0 -1 4 -2 -2 0 -1 -2 -2 0 67 0 0 0 0 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0.37 0.11 4 L -2 -2 -3 -4 -2 -2 -3 -4 -3 1 4 -2 4 0 -3 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 68 0 32 0 0 0 0 0 0 0 0.53 0.11 5 T 2 -2 -1 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -3 -1 1 4 -3 -2 0 33 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 67 0 0 0 0.46 0.11 6 V 4 -2 -2 -3 -1 -2 -2 -1 -2 0 -1 -2 -1 -2 -2 0 0 -3 -2 2 66 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 34 0.36 0.11 7 A 4 -2 -1 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -3 -1 1 3 -3 -2 0 68 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 32 0 0 0 0.43 0.11 8 A 4 -2 -1 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 3 0 -3 -2 -1 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0 33 0 0 0 0 0.46 0.11 9 T 0 -2 0 -1 -2 -1 -2 3 -2 -2 -2 -1 -2 -3 -2 1 4 -3 -2 -1 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 67 0 0 0 0.53 0.11 10 E -1 -1 0 4 -4 1 5 -2 -1 -4 -3 0 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 33 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0.80 0.11 11 V -1 -3 -3 -4 -1 -3 -3 -4 -3 2 2 -3 1 -1 -3 -2 -1 -3 -1 4 0 0 0 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 67 0.44 0.11 12 R 2 4 -1 -1 -2 4 0 -1 -1 -3 -2 1 -1 -3 -2 0 -1 -3 -2 -2 32 34 0 0 0 33 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.44 0.11 13 R -2 5 -1 -1 -4 1 3 -3 -1 -3 -3 1 -2 -3 -2 -1 -1 -3 -2 -3 0 68 0 0 0 0 32 0 0 0 0 0 0 0 0 0 0 0 0 0 0.71 0.11 14 I -2 -3 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.58 0.11 15 R -1 3 -1 -2 -3 -1 -2 5 -2 -4 -3 0 -3 -3 -2 -1 -2 -3 -3 -3 0 34 0 0 0 0 0 66 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.11 16 D -1 -2 0 3 -2 -1 0 -2 -2 -1 2 -1 0 -2 -2 0 3 -3 -2 -1 0 0 0 34 0 0 0 0 0 0 32 0 0 0 0 0 33 0 0 0 0.31 0.11 17 R 4 3 -1 -2 -1 0 -1 -1 -1 -2 -2 0 -1 -3 -2 0 -1 -3 -2 -1 66 34 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.43 0.11 18 A 2 -2 -3 -3 -1 -2 -2 -2 -3 3 2 -2 1 -1 -2 -1 -1 -3 -2 1 34 0 0 0 0 0 0 0 0 32 33 0 0 0 0 0 0 0 0 0 0.28 0.11 19 I -1 -2 3 -1 -2 -1 -1 -1 -1 2 -1 -1 -1 -2 -2 2 0 -3 -2 0 0 0 32 0 0 0 0 0 0 34 0 0 0 0 0 33 0 0 0 0 0.26 0.11 20 Q 2 0 -1 -1 -2 5 1 -1 0 -3 -2 0 -1 -3 -1 0 -1 -2 -2 -2 33 0 0 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.54 0.11 21 S 2 -2 -1 -1 -1 -1 -1 3 -2 -3 -3 -1 -2 -3 -2 3 0 -3 -2 -2 32 0 0 0 0 0 0 33 0 0 0 0 0 0 0 34 0 0 0 0 0.47 0.11 22 D -2 -2 4 5 -4 -1 1 -1 -1 -4 -4 -1 -3 -4 -2 0 -1 -4 -3 -3 0 0 33 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.87 0.11 23 A 4 -2 -2 -3 -1 -2 -2 -1 -2 0 2 -2 0 -1 -2 0 -1 -3 -2 0 68 0 0 0 0 0 0 0 0 0 32 0 0 0 0 0 0 0 0 0 0.33 0.11 24 Q 4 -1 -1 -2 -1 3 0 -1 -1 -2 -2 0 -1 -3 -1 0 -1 -3 -2 -1 66 0 0 0 0 34 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.43 0.11 25 V 2 -2 -3 -3 -1 -2 -2 -2 -3 1 0 -2 0 -2 -2 -1 0 -3 -2 4 33 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 67 0.35 0.11 26 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.11 27 P 2 -2 -2 -2 -2 -2 -2 3 -2 -3 -3 -1 -2 -3 5 0 -1 -3 -3 -2 33 0 0 0 0 0 0 32 0 0 0 0 0 0 34 0 0 0 0 0 0.76 0.11 28 M -1 -2 -2 -2 -3 -1 -2 -3 -2 -1 -1 -1 4 -2 6 -1 -1 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 34 0 66 0 0 0 0 0 1.24 0.11 29 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -2 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.65 0.11 30 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -2 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.65 0.11 31 A 2 -2 -1 -2 -2 -2 -2 5 -2 -3 -3 -2 -2 -3 -2 0 -1 -3 -3 -2 34 0 0 0 0 0 0 66 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.11 32 V -1 -3 -3 -4 -1 -3 -3 -4 -3 2 2 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 0 32 0 0 0 0 0 0 0 0 68 0.44 0.11 33 R 2 3 -2 -3 -2 -1 -1 -2 -2 -1 2 0 0 -2 -2 -1 -1 -3 -2 -1 32 34 0 0 0 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0 0.26 0.11 34 P 4 -2 -2 -2 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 5 0 -1 -3 -2 -1 66 0 0 0 0 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0 0.75 0.11 35 P 2 -2 -2 -2 -2 -1 -1 -2 -2 -3 -3 -1 -2 -3 7 0 -1 -4 -3 -2 32 0 0 0 0 0 0 0 0 0 0 0 0 0 68 0 0 0 0 0 1.33 0.11 36 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.11 37 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.11 38 D -2 -2 1 6 -4 -1 1 -2 -1 -4 -4 -1 -3 -4 -2 -1 -1 -5 -3 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.12 0.11 39 L -1 -1 -1 0 -2 0 3 -2 -1 -1 2 -1 0 -2 -2 2 0 -3 -2 -1 0 0 0 0 0 0 32 0 0 0 34 0 0 0 0 33 0 0 0 0 0.24 0.11 40 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.52 0.11 41 S 1 -1 0 -1 -1 0 0 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.60 0.11 42 E 2 -1 -1 0 -2 1 5 -1 -1 -3 -3 0 -2 -3 -1 0 -1 -3 -2 -2 33 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.11 43 K 2 4 -1 -2 -2 0 0 -1 -1 -3 -2 3 -2 -3 -2 0 -1 -3 -2 -2 32 33 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0 0 0 0 0.43 0.11 44 A 2 -2 -2 -2 -1 -2 -2 -2 -2 2 0 -2 0 -2 -2 0 3 -3 -2 1 34 0 0 0 0 0 0 0 0 32 0 0 0 0 0 0 33 0 0 0 0.26 0.11 45 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.11 46 T 2 -2 -1 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -3 -1 1 4 -3 -2 0 33 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 67 0 0 0 0.46 0.11 47 F -2 -3 -3 -4 -2 -3 -3 -4 -3 1 2 -3 1 4 -3 -2 -1 -1 1 2 0 0 0 0 0 0 0 0 0 0 32 0 0 34 0 0 0 0 0 33 0.42 0.11 48 L -2 -3 -4 -4 -2 -3 -3 -4 -2 0 2 -3 1 6 -4 -3 -2 0 2 0 0 0 0 0 0 0 0 0 0 0 34 0 0 66 0 0 0 0 0 0 0.66 0.11 49 V 2 -2 -1 3 -2 -1 0 -1 -2 0 -1 -2 -1 -2 -2 0 -1 -3 -2 2 33 0 0 32 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 34 0.28 0.11 50 E -1 -1 4 0 -3 0 3 3 -1 -4 -4 0 -3 -3 -2 0 -1 -3 -3 -3 0 0 32 0 0 0 34 33 0 0 0 0 0 0 0 0 0 0 0 0 0.55 0.11 51 Y -2 -2 -2 -3 -3 -2 -2 -3 1 -2 -1 -2 -1 3 -3 -2 -2 2 7 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.11 0.11 52 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.11 53 R -2 5 -1 -1 -4 4 0 -2 0 -3 -3 2 -1 -3 -2 -1 -1 -3 -2 -3 0 68 0 0 0 32 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.75 0.11 54 K -1 1 -1 -1 -3 4 0 3 -1 -3 -3 3 -2 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 33 0 32 0 0 0 34 0 0 0 0 0 0 0 0 0.50 0.11 55 Y -2 -2 -2 -3 -3 -2 -2 -3 1 -2 -1 -2 -1 3 -3 -2 -2 2 7 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.11 0.11 56 R -1 4 0 -1 -3 5 1 -2 0 -3 -3 1 -1 -3 -2 -1 -1 -3 -2 -3 0 34 0 0 0 66 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.71 0.11 57 Q 4 -1 -1 -2 -1 3 0 -1 -1 -2 -2 0 -1 -3 -1 0 -1 -3 -2 -1 66 0 0 0 0 34 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.43 0.11 58 T -1 -2 -2 -3 -1 -2 -2 -3 -3 1 2 -2 1 -1 -2 -1 3 -3 -2 2 0 0 0 0 0 0 0 0 0 0 33 0 0 0 0 0 34 0 0 32 0.30 0.11 59 I 0 -2 -1 -1 -1 -1 -1 -1 -2 2 -1 -1 -1 -2 -2 4 1 -3 -2 0 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0 66 0 0 0 0 0.30 0.11 60 A 4 3 -1 -2 -1 0 -1 -1 -1 -2 -2 0 -1 -3 -2 0 -1 -3 -2 -1 68 32 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.43 0.11 61 A 2 0 -1 -1 -2 5 1 -1 0 -3 -2 0 -1 -3 -1 0 -1 -2 -2 -2 34 0 0 0 0 66 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.53 0.11 62 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.11 63 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.56 0.11 64 V 4 -2 -2 -3 -1 -2 -2 -1 -2 0 -1 -2 -1 -2 -2 0 0 -3 -2 2 66 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 34 0.36 0.11 65 V -2 -3 -3 -4 -2 -3 -3 -4 -2 1 0 -3 0 6 -3 -2 -2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 66 0 0 0 0 0 34 0.60 0.11 66 L -2 -1 -1 -2 -3 -1 -1 -3 7 -1 2 -2 0 -1 -3 -2 -2 -2 1 -1 0 0 0 0 0 0 0 0 66 0 34 0 0 0 0 0 0 0 0 0 0.63 0.11 67 E -1 -1 0 4 -3 0 3 -1 -1 -3 -3 0 -2 -3 -1 2 0 -4 -3 -3 0 0 0 32 0 0 34 0 0 0 0 0 0 0 0 33 0 0 0 0 0.55 0.11 68 E 2 0 -1 0 -2 4 3 -1 -1 -3 -2 0 -1 -3 -1 0 -1 -3 -2 -2 32 0 0 0 0 33 34 0 0 0 0 0 0 0 0 0 0 0 0 0 0.46 0.11 69 F -3 -3 -3 -4 -3 -4 -4 -4 -2 0 0 -3 0 7 -4 -3 -2 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.98 0.11 70 A 4 -2 -2 -3 -1 -2 -2 -1 -2 0 -1 -1 -1 -2 -2 0 0 -3 -2 2 67 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 33 0.36 0.11 71 H -1 0 0 -1 -3 5 1 -2 6 -3 -3 0 -1 -3 -2 -1 -1 -2 0 -3 0 0 0 0 0 66 0 0 34 0 0 0 0 0 0 0 0 0 0 0 0.72 0.11 72 A 4 -2 -1 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -3 -1 1 3 -3 -2 0 68 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 32 0 0 0 0.43 0.11 73 L -2 -3 -4 -4 -2 -2 -3 -4 -3 1 4 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.62 0.11 74 T -1 -1 4 0 -2 -1 -1 -1 -1 -2 -2 -1 -2 -3 -2 1 4 -3 -2 -1 0 0 50 0 0 0 0 0 0 0 0 0 0 0 0 0 50 0 0 0 0.51 0.07 75 T 3 -2 -1 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -2 -1 1 4 -3 -2 0 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 50 0 0 0 0.39 0.07 76 G 3 -2 -1 -2 -2 -1 -2 4 -2 -3 -3 -1 -2 -3 -2 0 -1 -3 -3 -2 50 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 0 0 0 0 0.59 0.07 77 A 3 -2 -1 -2 -2 -1 -2 4 -2 -3 -3 -1 -2 -3 -2 0 -1 -3 -3 -2 50 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 0 0 0 0 0.59 0.07 K Lambda Standard Ungapped 0.1246 0.3175 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1367 0.3190 PSI Gapped 0.0418 0.2670