Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 I -2 -3 -4 -3 -1 -3 -4 -4 -4 5 2 -3 1 0 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.46 0.04 2 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.58 0.04 3 K -1 4 0 -1 -4 1 0 -2 -1 -3 -3 5 -2 -3 -2 -1 -1 -3 -2 -3 0 31 0 0 0 0 0 0 0 0 0 69 0 0 0 0 0 0 0 0 0.57 0.04 4 E -1 0 0 1 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.80 0.04 5 Q -1 1 0 -1 -3 5 1 -2 0 -3 -3 3 -1 -4 -2 0 -1 -3 -2 -3 0 0 0 0 0 69 0 0 0 0 0 31 0 0 0 0 0 0 0 0 0.66 0.10 6 K -1 2 0 -1 -4 3 1 -2 -1 -3 -3 5 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 22 0 0 0 0 0 78 0 0 0 0 0 0 0 0 0.61 0.10 7 L -1 -3 -4 -4 -2 -3 -3 -4 -3 2 4 -3 1 0 -3 -3 -1 -2 -1 3 0 0 0 0 0 0 0 0 0 0 69 0 0 0 0 0 0 0 0 31 0.47 0.10 8 I 1 -3 -3 -3 -1 -2 -3 -3 -3 3 1 -2 3 -1 -3 -1 -1 -3 -2 2 20 0 0 0 0 0 0 0 0 44 0 0 18 0 0 0 0 0 0 18 0.29 0.10 9 Q -2 -1 4 4 -4 3 2 -2 0 -4 -4 0 -2 -4 -2 0 -1 -4 -3 -3 0 0 30 28 0 25 17 0 0 0 0 0 0 0 0 0 0 0 0 0 0.58 0.11 10 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.57 0.11 11 Q -1 1 0 -1 -3 5 1 -2 0 -3 -3 3 -1 -4 -2 0 -1 -3 -2 -3 0 0 0 0 0 65 0 0 0 0 0 35 0 0 0 0 0 0 0 0 0.67 0.11 12 N -1 1 4 0 -3 3 0 -2 0 -3 -3 4 -2 -4 -2 0 -1 -4 -2 -3 0 0 38 0 0 17 0 0 0 0 0 45 0 0 0 0 0 0 0 0 0.53 0.11 13 L 2 -2 -2 -3 -1 -2 -2 -2 -3 0 3 -2 1 -1 -2 -1 1 -2 -2 0 28 0 0 0 0 0 0 0 0 0 55 0 0 0 0 0 17 0 0 0 0.27 0.11 14 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.53 0.11 15 R 1 3 3 -1 -2 0 -1 -1 -1 -3 -3 2 -2 -3 -2 0 2 -3 -2 -2 17 25 28 0 0 0 0 0 0 0 0 12 0 0 0 0 17 0 0 0 0.34 0.11 16 E -2 -1 -1 0 -4 1 5 -3 -1 -2 -2 0 -2 3 -2 -1 -2 -2 0 -2 0 0 0 0 0 0 72 0 0 0 0 0 0 28 0 0 0 0 0 0 0.57 0.11 17 F -3 -3 -4 -4 -3 -4 -4 -4 -2 0 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.98 0.11 18 E -1 1 -1 0 -4 3 4 -2 -1 -4 -3 3 -2 -4 -2 0 -1 -3 -2 -3 0 0 0 0 0 17 55 0 0 0 0 28 0 0 0 0 0 0 0 0 0.63 0.11 19 K -1 5 -1 -1 -4 1 0 -2 -1 -3 -3 4 -2 -3 -2 -1 -1 -3 -2 -3 0 45 0 0 0 0 0 0 0 0 0 55 0 0 0 0 0 0 0 0 0.69 0.11 20 T 0 -1 0 -1 -2 -1 -1 -2 -2 -1 -2 2 -1 -3 -1 1 5 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 17 0 0 0 0 83 0 0 0 0.52 0.11 21 H -2 4 0 -2 -4 0 0 -3 7 -4 -3 1 -2 -2 -3 -1 -2 -3 0 -3 0 45 0 0 0 0 0 0 55 0 0 0 0 0 0 0 0 0 0 0 0.78 0.11 22 T 0 -2 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -1 1 6 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.66 0.11 23 V -1 -3 -3 -4 -1 -3 -3 -4 -4 3 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 17 0 0 0 0 0 0 0 0 0 83 0.50 0.11 24 S 3 -1 -1 -1 -1 2 0 -1 -1 -2 -2 0 -1 -3 -1 3 0 -3 -2 -1 45 0 0 0 0 17 0 0 0 0 0 0 0 0 0 38 0 0 0 0 0.39 0.11 25 A 3 -2 -1 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -3 -1 1 4 -3 -2 0 55 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 45 0 0 0 0.43 0.11 26 H -2 -1 0 -2 -4 0 0 -3 9 -4 -3 -1 -2 -2 -3 -1 -2 -3 1 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 1.16 0.11 27 R -2 6 -1 -2 -4 1 0 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.95 0.11 28 K -1 2 -1 -1 -4 1 1 -2 -1 -3 -3 6 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.70 0.11 29 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.57 0.11 30 Q -1 1 0 -1 -3 6 2 -2 0 -3 -3 1 -1 -4 -2 0 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.11 31 K -2 5 -1 -2 -4 1 0 -2 -1 -3 -3 4 -2 -3 -2 -1 -1 -3 -2 -3 0 62 0 0 0 0 0 0 0 0 0 38 0 0 0 0 0 0 0 0 0.74 0.11 32 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.57 0.11 33 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.53 0.11 34 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -2 0 0 -4 -3 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.94 0.11 35 L -2 -3 -4 -4 -2 -3 -3 -4 -3 2 4 -3 2 0 -3 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 17 83 0 0 0 0 0 0 0 0 0 0.55 0.11 36 V -1 -3 -4 -4 -1 -3 -3 -4 -4 4 1 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 45 0 0 0 0 0 0 0 0 0 55 0.50 0.11 37 S 0 -1 0 -1 -2 0 0 -1 6 -3 -3 -1 -2 -2 -2 3 0 -3 0 -3 0 0 0 0 0 0 0 0 45 0 0 0 0 0 0 55 0 0 0 0 0.58 0.11 38 F -3 -3 -4 -4 -3 -4 -4 -4 -2 0 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.98 0.11 39 E -1 0 0 3 -4 4 5 -2 0 -4 -3 0 -2 -4 -2 0 -1 -3 -2 -3 0 0 0 17 0 28 55 0 0 0 0 0 0 0 0 0 0 0 0 0 0.69 0.11 40 Y -2 -2 -1 -3 -3 -1 -2 -3 5 -2 -2 -2 -2 2 -3 -2 -2 1 7 -2 0 0 0 0 0 0 0 0 28 0 0 0 0 0 0 0 0 0 72 0 0.91 0.11 41 K -1 1 3 0 -3 0 0 -1 -1 -3 -3 4 -2 -3 -2 2 0 -4 -2 -3 0 0 25 0 0 0 0 0 0 0 0 47 0 0 0 28 0 0 0 0 0.44 0.11 42 V 1 -2 -3 -3 -2 -2 -2 -3 0 0 -1 -2 -1 1 -3 -1 -1 0 5 3 17 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 45 38 0.41 0.11 43 K -1 1 -1 0 -4 3 4 -2 -1 -3 -3 3 -2 -4 -2 0 -1 -3 -2 -3 0 0 0 0 0 17 45 0 0 0 0 38 0 0 0 0 0 0 0 0 0.60 0.11 44 K -1 2 -1 -1 -4 1 1 -2 -1 -3 -3 6 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.70 0.11 45 M -1 0 -1 2 -3 0 0 -2 -1 -1 0 3 4 -2 -2 -1 -1 -3 -2 -1 0 0 0 17 0 0 0 0 0 0 0 45 38 0 0 0 0 0 0 0 0.31 0.11 46 V 1 0 -2 -2 -2 0 -1 -2 -2 1 -1 3 -1 -2 -2 -1 -1 -3 -2 2 17 0 0 0 0 0 0 0 0 13 0 45 0 0 0 0 0 0 0 25 0.22 0.11 47 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.62 0.11 48 Q -1 1 0 -1 -3 6 2 -2 0 -3 -3 1 -1 -4 -2 0 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.11 49 E -1 3 -1 0 -4 3 4 -2 0 -4 -3 3 -2 -4 -2 -1 -1 -3 -2 -3 0 28 0 0 0 17 38 0 0 0 0 17 0 0 0 0 0 0 0 0 0.57 0.11 50 R -2 5 -1 -1 -4 5 1 -3 0 -3 -3 2 -1 -3 -2 -1 -1 -3 -2 -3 0 55 0 0 0 45 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.71 0.11 51 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.58 0.11 52 D -2 -2 1 6 -4 0 3 -2 -1 -4 -4 -1 -3 -4 -2 -1 -1 -5 -3 -4 0 0 0 83 0 0 17 0 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.11 53 N -2 -2 4 0 -3 -1 1 -2 -1 1 2 -1 0 -2 -3 -1 -1 -3 -2 0 0 0 42 0 0 0 13 0 0 17 28 0 0 0 0 0 0 0 0 0 0.26 0.11 54 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.53 0.11 55 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.62 0.11 56 K -1 2 -1 -1 -4 1 1 -2 -1 -3 -3 6 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.70 0.11 57 Q -2 0 -1 -2 -3 5 1 -3 1 -3 -2 0 -1 1 -2 -1 -1 0 5 -2 0 0 0 0 0 59 0 0 0 0 0 0 0 0 0 0 0 0 41 0 0.62 0.12 58 G -1 -2 4 0 -3 -1 -2 5 -1 -4 -4 -1 -3 -4 -3 0 -1 -3 -3 -4 0 0 41 0 0 0 0 59 0 0 0 0 0 0 0 0 0 0 0 0 0.80 0.12 59 L -2 -3 -3 -3 -2 -2 -3 -3 -1 2 2 -2 0 3 -3 -1 2 -1 3 0 0 0 0 0 0 0 0 0 0 20 28 0 0 14 0 0 20 0 18 0 0.29 0.12 60 V -1 -3 -4 -4 -1 -3 -3 -4 -4 2 3 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 0 41 0 0 0 0 0 0 0 0 59 0.44 0.12 61 R -1 4 -1 -1 -4 1 0 -2 -1 -3 -3 5 -2 -4 -2 -1 -1 -3 -2 -3 0 28 0 0 0 0 0 0 0 0 0 72 0 0 0 0 0 0 0 0 0.67 0.12 K Lambda Standard Ungapped 0.1297 0.3177 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1510 0.3162 PSI Gapped 0.0462 0.2670