Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 E -2 -1 0 3 -4 4 5 -3 -1 -4 -4 0 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 17 0 29 53 0 0 0 0 0 0 0 0 0 0 0 0 0 0.78 0.21 2 L -2 -2 -3 -4 -2 2 -2 -4 -3 1 5 -2 1 -1 -3 -3 -2 -2 -2 0 0 0 0 0 0 18 0 0 0 0 82 0 0 0 0 0 0 0 0 0 0.59 0.24 3 F -3 -4 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 -1 8 -5 -3 -3 0 2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.25 0.24 4 S 0 -2 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 5 3 -4 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 76 24 0 0 0 0.66 0.24 5 V -1 -3 -3 -3 -2 -3 -3 -4 -4 2 0 -3 0 -2 -3 -1 3 -4 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 24 0 0 76 0.55 0.24 6 P 0 -2 -1 4 -3 0 3 -2 -2 -4 -4 -1 -3 -4 5 1 -1 -4 -3 -3 9 0 0 30 0 0 24 0 0 0 0 0 0 0 28 9 0 0 0 0 0.73 0.24 7 Y -3 3 -3 -4 -3 -2 -2 -4 0 -2 -2 -1 -2 4 -4 -2 -2 1 7 -2 0 24 0 0 0 0 0 0 0 0 0 0 0 18 0 0 0 0 58 0 0.86 0.24 8 F -3 -3 -4 -4 -3 -4 -4 -4 -2 0 2 -4 0 7 -4 -3 -3 0 2 -1 0 0 0 0 0 0 0 0 0 0 24 0 0 76 0 0 0 0 0 0 0.94 0.24 9 I -2 -1 -2 -2 -3 0 2 -3 -2 3 0 3 3 -2 -3 -2 -1 -3 -2 1 0 0 0 0 0 0 18 0 0 32 0 30 13 0 0 0 0 0 0 7 0.30 0.24 10 E 1 -1 -1 2 -3 1 5 -2 -1 -4 -3 0 -3 -4 -2 2 -1 -4 -3 -3 12 0 0 7 0 0 58 0 0 0 0 0 0 0 0 23 0 0 0 0 0.63 0.24 11 N 2 -1 6 0 -3 -1 -1 -1 -1 -3 -4 -1 -3 -4 -2 0 -1 -4 -3 -3 30 0 70 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.75 0.24 12 L -3 -3 -4 -4 -3 -4 -4 -4 -3 0 3 -4 0 6 -4 -3 -2 -1 1 -1 0 0 0 0 0 0 0 0 0 0 44 0 0 56 0 0 0 0 0 0 0.78 0.24 13 K -1 3 -1 -1 -3 3 0 -2 3 -3 -3 4 -2 -3 -2 -1 0 -3 -2 -2 1 13 0 1 0 19 1 1 10 1 1 41 0 0 1 1 8 0 0 1 0.47 0.21 14 Q -1 -1 -1 -1 -3 5 3 -2 -1 -2 1 0 -1 -2 -2 -1 0 -2 -2 -2 1 1 0 1 0 39 25 1 0 1 20 1 0 0 1 1 8 0 0 1 0.40 0.21 15 H -2 -1 -1 -2 -4 3 -1 -3 7 -3 -3 -1 -2 1 -3 -2 -2 0 6 -3 0 0 0 0 0 19 0 0 39 0 0 0 0 0 0 0 0 0 42 0 0.88 0.24 16 I -2 -3 -4 -4 -2 -3 -4 -4 -4 5 2 -3 3 -1 -4 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 76 11 0 13 0 0 0 0 0 0 0 0.67 0.24 17 E 0 2 -1 0 -4 4 4 -2 -1 -4 -3 2 -2 -4 -2 1 -1 -3 -3 -3 7 12 0 0 0 24 38 0 0 0 0 9 0 0 0 10 0 0 0 0 0.55 0.24 18 M -2 3 -2 -2 -3 3 0 -3 -1 1 -1 2 5 -2 -3 -1 -1 -3 -2 -1 0 18 0 0 0 24 0 0 0 11 0 19 28 0 0 0 0 0 0 0 0.40 0.24 19 N -2 -1 7 0 -4 0 -1 -1 3 -4 -4 1 -3 -4 -3 0 -1 -4 -2 -4 0 0 82 0 0 0 0 0 7 0 0 11 0 0 0 0 0 0 0 0 0.94 0.24 20 Q -1 0 2 0 -4 2 3 0 -1 -4 -4 3 -2 -4 3 -1 -1 -4 -3 -3 0 0 10 0 0 14 24 9 0 0 0 29 0 0 14 0 0 0 0 0 0.50 0.24 21 S 2 -2 4 4 -3 -1 -1 1 -1 -3 -4 -1 -3 -4 -2 1 -1 -4 -3 -3 24 0 33 27 0 0 0 10 0 0 0 0 0 0 0 6 0 0 0 0 0.52 0.24 22 E -2 1 -1 -1 -3 2 3 1 3 -2 -2 -1 3 1 -3 -1 -2 -3 -1 0 0 10 0 0 0 9 28 11 12 0 0 0 13 9 0 0 0 0 0 9 0.25 0.24 23 D -1 -2 0 4 -2 -1 0 -2 -2 -3 -3 -1 -2 -4 -2 3 3 -4 -3 -2 0 0 0 39 0 0 0 0 0 0 0 0 0 0 0 27 34 0 0 0 0.60 0.24 24 K -2 2 -1 -2 -4 0 0 -3 -2 -4 -3 5 -2 -4 5 -1 -2 -4 -3 -3 0 7 0 0 0 0 0 0 0 0 0 63 0 0 30 0 0 0 0 0 0.88 0.24 25 I 0 1 2 -2 -2 -2 -2 -3 -2 4 0 -2 0 -2 -3 -2 -1 -4 -2 2 9 12 17 0 0 0 0 0 0 45 0 0 0 0 0 0 0 0 0 18 0.31 0.24 26 H -2 0 0 -1 -4 5 0 -3 8 -4 -3 0 -2 -3 -3 -1 -2 -3 0 -4 0 0 0 0 0 43 0 0 57 0 0 0 0 0 0 0 0 0 0 0 1.00 0.24 27 A 4 -2 -2 -2 -1 -1 -2 -1 -2 -2 -2 -1 -2 -3 -2 1 3 -3 -3 -1 70 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30 0 0 0 0.57 0.24 28 M -2 -2 -3 -4 -2 -2 -3 -4 -3 1 3 -2 7 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 34 0 66 0 0 0 0 0 0 0 0.74 0.24 29 N -2 -1 7 1 -4 -1 -1 -1 0 -4 -4 -1 -3 -4 -3 0 -1 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.17 0.24 30 S 4 -2 -1 -2 -1 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 4 0 -4 -3 -2 49 0 0 0 0 0 0 0 0 0 0 0 0 0 0 51 0 0 0 0 0.58 0.24 31 Y -3 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 2 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.38 0.24 32 Y -3 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 2 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.38 0.24 33 R -2 5 -1 -2 -4 0 -1 -3 6 -4 -3 3 -2 -3 -3 -1 -2 -4 -1 -3 0 45 0 0 0 0 0 0 30 0 0 24 0 0 0 0 0 0 0 0 0.79 0.24 34 S 0 -2 -1 -2 -2 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 4 4 -3 -2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 45 42 0 0 13 0.51 0.24 35 V -1 -3 -4 -4 -2 -3 -3 -4 -4 3 0 -3 0 -1 -3 -3 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 18 0 0 0 0 0 0 0 0 0 82 0.68 0.24 36 V 2 -3 -3 -4 -1 -3 -3 -3 -4 2 0 -3 0 -2 -3 -1 -1 -4 -2 5 24 0 0 0 0 0 0 0 0 11 0 0 0 0 0 0 0 0 0 65 0.49 0.24 37 S 0 -2 0 -1 -2 -1 -2 4 -2 -4 -4 -1 -3 -4 -2 4 0 -4 -3 -3 0 0 0 0 0 0 0 42 0 0 0 0 0 0 0 58 0 0 0 0 0.71 0.24 38 T 3 -2 -1 -2 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 -2 3 4 -3 -2 -1 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 24 45 0 0 0 0.52 0.24 39 L -2 -3 -4 -4 -2 -3 -4 -5 -4 5 4 -3 1 -1 -4 -3 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 55 45 0 0 0 0 0 0 0 0 0 0.65 0.24 40 V -2 -3 -4 -4 -2 -3 -4 -4 -4 4 2 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 43 25 0 0 0 0 0 0 0 0 32 0.54 0.20 41 Q 1 0 2 -1 -3 6 1 -2 0 -3 -3 0 -1 -4 -2 1 -1 -3 -2 -3 13 0 13 0 0 62 0 0 0 0 0 0 0 0 0 12 0 0 0 0 0.59 0.20 42 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.27 0.20 43 Q -2 5 -1 -2 -4 4 0 -3 4 -4 -3 1 -2 -3 -3 -1 -2 -3 -2 -3 0 55 0 0 0 32 0 0 13 0 0 0 0 0 0 0 0 0 0 0 0.75 0.20 44 L -2 -3 -4 -4 -2 -3 -4 -5 -4 4 3 -3 1 0 -4 -3 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 55 45 0 0 0 0 0 0 0 0 0 0.60 0.20 45 T 2 -1 5 0 -2 -1 -1 -1 -1 -2 -3 -1 -2 -3 -2 1 3 -4 -3 -2 25 0 43 0 0 0 0 0 0 0 0 0 0 0 0 0 32 0 0 0 0.50 0.20 46 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.84 0.20 47 N -2 -1 7 1 -3 -1 -1 -1 0 -4 -4 -1 -3 -4 -3 0 -1 -5 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.10 0.20 48 A 3 -3 -2 -3 -2 -2 -2 1 -2 -2 -2 -2 -1 4 -2 1 -1 -2 0 -1 48 0 0 0 0 0 0 13 0 0 0 0 0 30 0 8 0 0 0 0 0.39 0.20 49 V -1 -2 -1 3 -3 0 4 -3 -2 -1 -2 -1 -2 -3 -2 -1 -1 -4 -3 2 0 0 0 25 0 0 43 0 0 0 0 0 0 0 0 0 0 0 0 32 0.47 0.20 50 V -2 -3 -4 -4 -2 -3 -4 -4 -4 3 4 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 13 55 0 0 0 0 0 0 0 0 32 0.53 0.20 51 L -2 -3 -4 -4 -2 -3 -4 -4 -4 5 3 -3 1 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 55 32 0 0 0 0 0 0 0 0 13 0.57 0.20 52 K -1 5 -1 -2 -3 0 -1 -3 -2 -1 -2 3 -1 -3 -2 -1 -1 -4 -2 2 0 43 0 0 0 0 0 0 0 0 0 32 0 0 0 0 0 0 0 25 0.50 0.20 53 R -2 7 -1 -2 -4 0 -1 -3 -1 -4 -3 2 -2 -4 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.10 0.20 54 I -2 -3 -4 -4 -2 -3 -4 -4 -3 5 1 -3 4 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 75 0 0 25 0 0 0 0 0 0 0 0.62 0.20 55 Q -2 6 -1 -2 -4 4 0 -3 -1 -4 -3 1 -2 -4 -3 -1 -2 -3 -2 -3 0 68 0 0 0 32 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.86 0.20 56 H -2 -1 5 0 -4 0 -1 -2 7 -4 -4 -1 -2 -3 -3 -1 -1 -4 0 -4 0 0 43 0 0 0 0 0 57 0 0 0 0 0 0 0 0 0 0 0 0.96 0.20 57 L -2 -3 -4 -4 -2 -3 -4 -4 -4 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.75 0.20 58 D -2 -2 0 6 -4 0 4 -2 -1 -4 -4 -1 -3 -4 -2 -1 -2 -4 -3 -4 0 0 0 62 0 0 38 0 0 0 0 0 0 0 0 0 0 0 0 0 0.96 0.20 59 E -1 -1 -1 0 -3 1 5 -3 -1 -3 -3 0 -2 -4 -2 0 3 -3 -3 -2 0 0 0 0 0 0 70 0 0 0 0 0 0 0 0 0 30 0 0 0 0.70 0.20 60 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -2 -3 -1 1 -1 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.69 0.20 61 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 3 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.30 0.20 62 N -2 0 5 0 -4 4 0 -2 0 -4 -4 1 -2 -4 -2 0 -1 -4 -3 -3 0 0 57 0 0 33 0 0 0 0 0 10 0 0 0 0 0 0 0 0 0.72 0.20 63 K 1 0 2 -1 -3 0 1 -2 3 -3 -3 4 -2 -3 -2 0 -1 -4 -2 -3 20 0 13 0 0 0 11 0 12 0 0 44 0 0 0 0 0 0 0 0 0.41 0.20 64 V -1 -3 -4 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.67 0.20 65 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.75 0.14 66 R -1 5 -1 -1 -4 1 0 -2 -1 -3 -3 4 -2 -3 -2 -1 -1 -3 -2 -3 0 49 0 0 0 0 0 0 0 0 0 51 0 0 0 0 0 0 0 0 0.66 0.08 67 G 0 -3 0 -1 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 0 -2 -3 -3 -3 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.06 0.01 K Lambda Standard Ungapped 0.1306 0.3169 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1668 0.3169 PSI Gapped 0.0511 0.2670