Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.72 0.18 2 P -2 -3 -3 -3 -4 -2 -2 -3 -3 -4 -4 -2 -4 -5 8 -2 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.43 0.35 3 E -2 -1 -1 2 -4 1 5 -3 -1 -4 -4 2 -3 -4 -2 1 -1 -4 -3 -3 0 0 0 13 0 0 61 0 0 0 0 13 0 0 0 13 0 0 0 0 0.79 0.35 4 S 1 -2 -1 -2 -2 -2 -2 -2 -3 -2 -2 -2 -2 1 3 2 4 -3 -2 -2 13 0 0 0 0 0 0 0 0 0 0 0 0 9 13 19 46 0 0 0 0.53 0.35 5 P -2 -3 -3 -3 -4 -2 -2 -3 -3 -4 -4 -2 -4 -5 8 -2 -2 -5 -4 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.47 0.40 6 K -2 1 -1 -2 -4 3 0 -3 -2 -4 -4 6 -2 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 11 0 0 0 0 0 89 0 0 0 0 0 0 0 0 1.03 0.40 7 R -2 2 0 -2 -3 -1 -2 -3 3 -3 -3 -1 -2 -3 -3 0 5 4 -2 -2 0 15 3 0 0 0 0 0 11 0 0 0 0 0 0 0 63 8 0 0 0.71 0.40 8 A 4 -3 -2 -3 -2 -2 -2 -2 -3 -2 -2 -2 -2 -3 3 1 1 -4 -3 1 55 0 0 0 0 0 0 0 0 0 0 0 0 0 13 11 8 0 0 13 0.55 0.40 9 E -1 2 -1 0 -5 4 5 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -4 2 11 0 0 0 24 62 0 0 0 0 0 0 0 0 0 0 0 0 0 0.92 0.40 10 E -2 -1 -1 1 -4 0 6 -3 -1 -4 -4 -1 -3 -4 -2 -1 1 -4 -3 -3 0 0 0 4 0 0 76 0 0 0 0 0 0 0 0 0 11 0 0 0 0.87 0.40 11 I -2 -4 -4 -4 -2 -3 -4 -5 -4 5 3 -3 5 -1 -4 -3 -1 -3 -2 1 0 0 0 0 0 0 0 0 0 56 22 0 21 0 0 0 2 0 0 0 0.74 0.40 12 W -4 1 -4 -5 -4 -3 -4 -4 -3 -4 -3 -3 -2 2 -5 -4 -3 11 1 -4 0 11 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 82 0 0 2.50 0.40 13 Q -2 0 -1 2 -4 7 1 -3 -1 0 -3 0 -1 -4 -3 -1 -2 -3 -3 -2 0 0 0 11 0 78 0 0 0 10 0 0 0 0 0 0 0 0 0 0 0.95 0.40 14 Q -2 1 -1 -1 -4 6 2 -3 3 -4 -4 0 -2 -4 2 -1 -2 -3 -2 -4 0 4 0 0 0 64 10 0 10 0 0 0 0 0 11 0 0 0 0 0 0.92 0.40 15 S 0 -2 0 -1 -2 -1 -1 -1 -2 -3 -4 -1 -3 -4 2 5 1 -4 -3 -3 0 0 2 0 0 0 0 3 0 0 0 0 0 0 11 77 7 0 0 0 0.78 0.40 16 V -1 -2 -3 -4 -2 -2 -3 -4 -4 3 0 2 -1 -2 -3 -3 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 12 0 17 0 0 0 0 0 0 0 72 0.66 0.40 17 I -2 -4 -4 -4 4 -3 -4 -4 -4 5 1 -4 1 -2 -4 -2 1 -4 -3 2 0 0 0 0 11 0 0 0 0 61 7 0 2 0 0 0 12 0 0 7 0.67 0.40 18 G -1 -3 -2 -2 3 -2 1 5 -3 -3 -4 -2 -3 -4 2 1 -2 -4 -4 -1 0 0 0 0 8 0 10 48 0 0 0 0 0 0 11 17 0 0 0 7 0.63 0.40 19 D -2 -2 4 5 -4 0 3 -2 -1 -4 -4 -1 -3 -4 -2 -1 -2 -4 -3 -3 1 1 21 48 0 0 21 1 0 1 1 1 0 0 1 1 1 0 0 1 0.82 0.35 20 Y -2 -2 -3 -3 3 -2 -3 -3 1 -2 -2 -3 -2 2 -3 -2 -2 1 8 -2 1 1 1 1 7 0 1 1 0 1 1 1 0 0 1 1 1 0 82 1 1.25 0.35 21 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 -1 -3 -3 -2 -2 -2 2 1 1 1 1 0 0 1 1 0 1 75 1 3 0 1 1 1 0 0 13 0.65 0.35 22 A 5 -2 -2 -2 -2 2 -1 -1 -2 -1 -2 -1 -1 -3 -2 0 -1 -3 -2 1 69 1 1 1 0 11 1 1 0 1 1 1 0 0 1 1 1 0 0 11 0.55 0.35 23 R -2 4 -1 -2 4 2 -1 -3 -1 -3 -3 4 -2 -3 -2 -1 -2 -3 -2 -3 1 31 1 1 13 8 1 1 0 1 1 38 0 0 1 1 1 0 0 1 0.65 0.35 24 F -2 -3 1 -3 1 -3 -3 -3 -1 -1 0 -3 -1 6 -3 -2 -2 0 5 -2 1 1 11 1 4 0 1 1 0 1 9 1 0 40 1 1 1 0 27 1 0.69 0.35 25 K -2 4 -1 -2 -3 2 0 -2 -1 -3 -3 5 -2 -3 -2 -1 -2 -3 -2 -3 1 23 1 1 0 12 1 1 0 1 1 54 0 0 1 1 1 0 0 1 0.71 0.35 26 N -1 -1 6 0 -3 -1 -1 -1 -1 -2 -3 -1 -2 -3 -2 2 -1 -4 -2 0 1 1 60 1 0 1 1 1 0 1 1 1 0 0 1 19 1 0 0 11 0.69 0.35 27 D -2 -2 2 6 -3 -1 0 -2 -2 -3 -3 -1 -3 -4 -2 -1 1 -4 -3 -3 1 1 8 70 0 1 1 1 0 1 1 1 0 0 1 1 11 0 0 1 0.94 0.35 28 R -2 6 -1 -2 -3 2 -1 -3 -1 -2 -2 2 -2 -3 -2 -1 -2 -3 -2 0 1 60 1 1 0 8 1 1 0 1 1 7 0 0 1 5 1 0 0 11 0.68 0.35 29 V 0 2 -2 -2 -2 -1 -2 1 -2 0 -2 2 -1 -3 1 0 0 -3 -2 2 7 14 1 1 0 1 1 13 0 2 1 17 0 0 7 4 4 0 0 27 0.21 0.35 30 K -2 1 -1 -1 -3 0 0 -2 -2 -3 -3 6 -2 -3 -2 -1 -1 -3 -2 -3 1 1 1 1 0 1 1 1 0 1 1 88 0 0 1 1 1 0 0 1 0.91 0.35 31 A 5 -2 -2 -2 -1 -2 -2 -1 -2 -2 -2 -2 -2 -3 -2 0 0 -3 -2 -1 85 1 1 1 0 1 1 1 0 1 1 1 0 0 1 1 4 0 0 1 0.73 0.35 32 L -2 -1 2 -2 -3 4 -1 -3 0 -1 3 -1 0 -2 1 -2 -2 -3 -2 -1 1 1 11 1 0 32 1 1 2 3 35 1 0 0 8 1 1 0 0 1 0.37 0.35 33 K 1 0 0 -1 -3 0 1 -2 -2 -2 -3 5 -2 -3 -2 0 -1 -3 -2 -1 11 1 3 1 0 1 11 1 0 1 1 55 0 0 1 4 2 0 0 6 0.48 0.35 34 A 5 -2 -2 -2 3 -2 -2 0 -2 -2 -2 -2 -2 -3 -2 0 0 -3 -2 -1 74 1 1 1 7 1 1 4 0 1 1 1 0 0 1 3 3 0 0 1 0.63 0.35 35 M -2 -2 -3 -3 -2 -1 -3 -3 -2 1 2 -2 8 -1 -3 -2 -2 -2 -2 0 1 1 1 1 0 1 1 1 0 3 18 1 69 0 1 1 1 0 0 1 0.83 0.35 36 E -2 -1 -1 2 -4 1 5 -2 -1 -3 -3 2 -3 -3 -2 -1 -2 -3 -3 -3 1 1 1 11 0 1 60 1 0 1 1 19 0 0 1 1 1 0 0 1 0.75 0.35 37 M 3 -2 -2 1 -2 -1 -1 -1 -2 -1 -1 -2 5 -2 -2 1 1 -3 -2 -1 35 1 1 11 0 1 1 3 0 1 1 1 26 0 1 7 9 0 0 2 0.34 0.35 38 T 2 -2 -1 -2 -2 2 1 -2 -2 -2 -2 -1 -2 -3 -2 0 5 -3 -2 -1 23 1 1 1 0 11 7 1 0 1 1 1 0 0 1 1 47 0 0 3 0.45 0.35 39 W -3 0 -3 -4 -3 -2 -3 -3 -3 -3 -2 -2 -2 0 -3 -3 -3 11 1 -3 1 8 1 1 0 1 1 1 0 1 1 1 0 0 1 1 1 80 0 1 2.30 0.35 40 N 1 0 5 -1 -3 -1 -1 -2 -1 -2 0 2 -2 -3 -2 2 -1 -3 -2 -2 11 3 34 1 0 1 1 1 0 1 10 20 0 0 1 15 1 0 0 1 0.37 0.35 41 N 1 2 4 -1 -2 -1 -1 0 -1 -3 -3 1 -2 -3 -2 1 2 -3 -2 -2 16 11 27 1 0 1 1 8 0 1 1 9 0 0 1 10 13 0 0 1 0.33 0.35 42 M -2 -2 -3 -3 -2 -2 -3 -3 -2 0 2 -2 7 -1 -3 -2 1 -2 -2 0 1 1 1 1 0 1 1 1 0 1 22 1 59 0 1 1 9 0 0 1 0.71 0.35 43 E -2 -1 -1 4 -4 0 6 -2 -1 -3 -3 0 -3 -4 -2 -1 -2 -3 -3 -3 1 1 1 21 0 1 68 1 0 1 1 1 0 0 1 1 1 0 0 1 0.89 0.35 44 K -2 1 -1 -2 -4 0 0 -3 -2 -4 -3 6 -2 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 96 0 0 0 0 0 0 0 0 1.06 0.38 45 K 1 0 -1 -1 -2 0 0 -1 -1 -2 -2 5 -1 -2 -1 -1 -1 -2 -2 -2 17 1 1 1 1 1 2 2 1 1 2 51 1 1 1 2 2 0 1 2 0.32 0.25 46 E -1 0 -1 0 -2 2 4 -2 -1 -3 -2 1 -2 -3 -1 2 -1 -2 -2 -2 2 1 1 1 1 9 45 2 1 1 2 9 1 1 1 17 2 0 1 2 0.45 0.25 47 K -1 1 2 -1 -3 0 0 -2 -1 -2 -2 5 -2 -3 -1 -1 -1 -2 -2 -2 2 1 13 1 1 1 2 2 1 1 2 64 1 1 1 2 2 0 1 2 0.54 0.25 48 L -2 -2 -3 -3 -2 -2 -3 -3 -3 4 3 -2 1 0 -2 -2 -1 -2 -2 1 2 1 1 1 0 1 1 1 0 42 41 1 0 1 1 1 1 0 1 1 0.41 0.21 49 M -2 -3 -3 -3 -3 -2 -3 -3 -3 0 0 -2 7 1 4 -2 -2 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 60 7 27 0 0 0 0 6 0.76 0.26 50 W -3 -3 -3 -4 -3 -2 -3 -3 -3 -3 -2 -3 -2 0 -3 -3 -2 11 1 -3 1 1 1 1 0 1 1 1 0 1 1 1 0 1 1 1 1 85 0 1 2.31 0.22 51 I -2 -3 -3 -3 -2 2 -2 -4 -3 6 1 -2 0 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 14 0 0 0 86 0 0 0 0 0 0 0 0 0 0 0.60 0.23 52 K -1 1 -1 -1 -4 2 2 -2 -1 -4 -3 5 -2 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 10 16 0 0 0 0 74 0 0 0 0 0 0 0 0 0.73 0.23 53 K 0 1 -1 -1 -4 1 2 -2 -1 -3 -3 5 -2 -4 -2 -1 -1 -4 -3 -3 8 0 0 0 0 0 14 0 0 0 0 78 0 0 0 0 0 0 0 0 0.69 0.23 54 A 5 -2 -2 -3 -1 -2 -2 2 -3 -2 0 -2 -1 -3 -2 0 -1 -3 -3 -1 74 0 0 0 0 0 0 16 0 0 10 0 0 0 0 0 0 0 0 0 0.52 0.23 55 A 5 -2 -2 -2 -1 -1 -2 0 -2 -2 -2 -1 -2 -3 -1 1 -1 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.71 0.21 56 E -1 0 0 0 -4 1 5 -3 -1 -4 -4 3 -2 -4 -2 -1 -1 -4 -3 -3 0 0 5 0 0 0 64 0 0 0 0 32 0 0 0 0 0 0 0 0 0.74 0.21 57 D -2 -2 1 6 -4 2 1 -2 -1 -4 -4 -1 -3 -4 -2 -1 -2 -5 -3 -4 0 0 0 82 0 18 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.04 0.21 58 Q -2 3 -1 -1 -4 6 1 -3 0 -4 -3 3 -1 -4 -2 -1 -1 -3 -2 -3 0 23 0 0 0 59 0 0 0 0 0 18 0 0 0 0 0 0 0 0 0.75 0.21 59 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.81 0.18 60 R -2 6 3 -1 -4 0 -1 -2 -1 -4 -3 1 -2 -4 -3 -1 -1 -4 -2 -3 0 81 19 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.87 0.18 61 Y -2 -2 -2 -3 -2 -2 -2 -3 0 2 -1 -2 -1 2 -2 -2 -2 1 7 0 1 1 1 1 0 1 1 1 0 20 2 1 0 4 1 1 1 0 62 1 0.62 0.15 62 E -1 -1 -1 2 -3 1 5 -2 -1 -3 -3 0 -2 -3 -1 -1 -1 -3 -2 -2 1 1 1 4 0 1 80 1 0 1 2 1 0 1 1 1 1 0 1 1 0.72 0.15 63 R -2 7 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.05 0.17 64 E -1 -1 0 2 -4 1 6 -3 -1 -4 -3 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 9 0 0 91 0 0 0 0 0 0 0 0 0 0 0 0 0 0.87 0.12 65 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 0 95 0 0 0 0 0 0 0 0 5 0.61 0.12 66 S 1 -2 0 -1 4 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 4 2 -3 -3 -2 5 0 0 0 11 0 0 0 0 0 0 0 0 0 0 67 17 0 0 0 0.58 0.23 K Lambda Standard Ungapped 0.1261 0.3126 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1587 0.3171 PSI Gapped 0.0486 0.2670