Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -1 -2 -2 -3 -2 -1 -2 -3 -2 1 2 -2 6 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.43 0.04 2 L 0 -2 -1 -2 -1 -1 -1 -2 -2 0 2 -1 1 -1 -2 3 0 -2 -2 0 0 0 0 0 0 0 0 0 0 0 50 0 0 0 0 50 0 0 0 0 0.21 0.04 3 F -2 -3 -3 -4 -2 -3 -3 -4 -2 3 1 -3 1 5 -3 -3 -2 0 1 1 0 0 0 0 0 0 0 0 0 50 0 0 0 50 0 0 0 0 0 0 0.44 0.04 4 S 0 -1 3 0 -2 0 0 -1 -1 -3 -3 0 -2 -3 -1 4 1 -3 -2 -2 0 0 28 0 0 0 0 0 0 0 0 0 0 0 0 72 0 0 0 0 0.50 0.08 5 I -2 -3 -4 -4 -2 -3 -3 -4 -3 3 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 25 75 0 0 0 0 0 0 0 0 0 0.50 0.08 6 Q -1 1 0 -1 -3 6 1 -2 4 -3 -3 1 -1 -3 -2 -1 -1 -2 -1 -3 0 0 0 0 0 78 0 0 22 0 0 0 0 0 0 0 0 0 0 0 0.71 0.09 7 T -1 -2 -1 -2 -1 -1 -2 -2 -2 1 -1 -1 0 -2 -2 0 5 -3 -2 2 0 0 0 0 0 0 0 0 0 14 0 0 0 0 0 0 71 0 0 15 0.44 0.13 8 C -1 -4 -3 -4 10 -3 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.62 0.15 9 P -1 -3 -2 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.22 0.15 10 C -1 -4 -3 -4 10 -3 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.62 0.15 11 Q -1 1 0 -1 -3 6 2 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.92 0.15 12 I -1 -3 -3 -4 -2 -3 -3 -4 -3 4 1 -3 3 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 60 0 0 12 0 0 0 0 0 0 28 0.51 0.15 13 N -1 -1 5 0 -3 0 -1 -1 5 -4 -4 -1 -2 -3 -2 2 0 -4 -1 -3 0 0 60 0 0 0 0 0 23 0 0 0 0 0 0 17 0 0 0 0 0.68 0.15 14 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -2 0 -2 -2 -3 7 -1 -2 -4 -3 -2 0 0 0 0 0 0 0 0 0 0 13 0 0 0 87 0 0 0 0 0 1.79 0.15 15 A 1 -2 -1 -1 -1 -1 -1 -1 -2 0 -2 -1 -1 -2 -2 3 4 -3 -2 0 12 0 0 0 0 0 0 0 0 12 0 0 0 0 0 35 41 0 0 0 0.37 0.15 16 L 2 -2 -1 -2 -1 -1 -1 -1 -2 -2 0 -1 -1 -2 3 3 1 -3 -2 -1 24 0 0 0 0 0 0 0 0 0 12 0 0 0 17 36 12 0 0 0 0.37 0.15 17 N -1 -1 4 2 -3 2 3 1 -1 -4 -4 0 -2 -4 -2 1 -1 -4 -3 -3 0 0 29 12 0 12 25 11 0 0 0 0 0 0 0 12 0 0 0 0 0.46 0.15 18 A 3 -2 0 1 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 -1 3 3 -3 -2 -1 35 0 0 12 0 0 0 0 0 0 0 0 0 0 0 28 25 0 0 0 0.37 0.15 19 V -1 -3 -3 -3 -2 -2 -3 -3 -3 3 2 -2 1 -1 -3 -1 2 -3 -2 2 0 0 0 0 0 0 0 0 0 35 28 0 0 0 0 0 25 0 0 12 0.35 0.15 20 S 0 -1 0 3 -2 -1 0 -1 -1 -3 -3 -1 -2 -3 -1 4 1 -4 -2 -2 0 0 0 25 0 0 0 0 0 0 0 0 0 0 0 75 0 0 0 0 0.57 0.15 21 T 3 -1 -1 -1 -1 2 0 -1 -1 -2 -2 -1 -1 -3 -1 3 2 -3 -2 -1 37 0 0 0 0 17 0 0 0 0 0 0 0 0 0 35 12 0 0 0 0.38 0.15 22 P -1 -3 -2 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.22 0.15 23 L -2 -3 -4 -4 -2 -3 -3 -4 -3 2 4 -3 2 0 -3 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 17 83 0 0 0 0 0 0 0 0 0 0.60 0.15 24 L 1 -2 -2 -3 -1 -2 -2 -2 -2 0 3 -2 1 -1 -2 2 -1 -3 -2 0 17 0 0 0 0 0 0 0 0 0 59 0 0 0 0 25 0 0 0 0 0.29 0.15 25 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -2 -1 3 -3 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.19 0.15 26 Q -1 1 0 -1 -3 5 1 -2 -1 -3 -3 3 -1 -4 -2 1 -1 -3 -2 -3 0 0 0 0 0 49 0 0 0 0 0 35 0 0 0 17 0 0 0 0 0.56 0.15 27 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.18 0.15 28 C -1 -4 -3 -4 10 -3 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.62 0.15 29 C -1 -4 -3 -4 10 -3 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.62 0.15 30 Q -1 1 0 -1 -3 6 2 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.92 0.15 31 P -1 -3 -2 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.22 0.15 32 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -2 -1 3 -3 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.19 0.15 33 H -2 -1 0 -2 -4 0 -1 -3 9 -4 -3 -1 -2 -2 -3 -1 -2 -3 1 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 1.25 0.15 34 D -2 -1 1 6 -4 0 2 -2 -1 -4 -4 2 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 71 0 0 12 0 0 0 0 17 0 0 0 0 0 0 0 0 0.84 0.15 35 G 3 -2 -1 -2 -2 -1 -2 4 -2 -3 -3 -1 -2 -3 -2 2 0 -3 -3 -2 37 0 0 0 0 0 0 39 0 0 0 0 0 0 0 23 0 0 0 0 0.54 0.15 36 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 4 -3 1 4 -3 -3 -2 -1 0 1 0 0 0 0 0 0 0 0 0 0 63 0 0 25 0 0 0 0 0 12 0.50 0.15 37 Y -2 -2 -3 -3 -2 -2 -3 1 0 -1 2 -2 0 2 -3 -2 -2 1 6 -1 0 0 0 0 0 0 0 14 0 0 29 0 0 0 0 0 0 0 57 0 0.54 0.13 38 N -1 -1 6 1 -2 0 -1 -1 0 -3 -4 -1 -2 -3 -2 2 0 -4 -2 -3 0 0 71 0 0 0 0 0 0 0 0 0 0 0 0 29 0 0 0 0 0.71 0.13 39 Q -1 0 0 1 -4 4 5 -2 0 -3 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 26 74 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.08 K Lambda Standard Ungapped 0.1392 0.3258 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1485 0.3150 PSI Gapped 0.0454 0.2670