Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.58 0.11 2 F -3 -3 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.98 0.11 3 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.58 0.11 4 S 1 -1 0 -1 -1 0 0 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.60 0.11 5 N -2 -1 4 4 -3 -1 0 -1 -1 -3 -3 -1 -2 -3 -2 0 3 -4 -3 -2 0 0 39 33 0 0 0 0 0 0 0 0 0 0 0 0 28 0 0 0 0.56 0.11 6 D -2 -2 2 6 -4 -1 1 -2 -1 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 10 90 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.02 0.11 7 K -1 2 -1 -1 -4 1 1 -2 -1 -3 -3 6 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.69 0.11 8 E -2 -1 4 4 -4 1 4 -2 0 -4 -4 0 -3 -4 -2 0 -1 -4 -3 -3 0 0 28 24 0 0 48 0 0 0 0 0 0 0 0 0 0 0 0 0 0.66 0.11 9 S 1 -1 0 -1 -1 0 0 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.60 0.11 10 I -2 -3 -4 -4 -2 -3 -4 -4 -3 3 4 -3 2 0 -3 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 22 78 0 0 0 0 0 0 0 0 0 0.54 0.11 11 K -1 1 4 0 -3 1 0 -2 -1 -3 -3 5 -2 -4 -2 0 -1 -4 -2 -3 0 0 36 0 0 0 0 0 0 0 0 64 0 0 0 0 0 0 0 0 0.56 0.11 12 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.52 0.11 13 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.17 0.11 14 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.52 0.11 15 E 0 -1 0 4 -4 1 5 -2 -1 -4 -3 0 -3 -4 -2 0 -1 -4 -3 -3 8 0 0 27 0 0 65 0 0 0 0 0 0 0 0 0 0 0 0 0 0.70 0.11 16 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.17 0.11 17 E -1 -1 -1 0 -3 1 4 -2 -1 -2 -3 0 -2 -3 -2 0 3 -3 -2 -2 0 0 0 0 0 0 57 0 0 0 0 0 0 0 0 0 43 0 0 0 0.53 0.11 18 R -2 2 -1 -2 -3 4 0 -3 5 -1 -2 1 1 -2 -2 -1 -1 -3 -1 -2 0 14 0 0 0 33 0 0 25 8 0 10 9 0 0 0 0 0 0 0 0.38 0.11 19 I -2 -3 -4 -4 -2 -3 -3 -4 -3 4 3 -3 3 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 39 34 0 10 0 0 0 0 0 0 17 0.43 0.11 20 S 0 -1 0 -1 -1 -1 -1 -1 -2 -2 -2 -1 -1 -3 -1 4 4 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 49 51 0 0 0 0.50 0.11 21 E -2 0 5 1 -4 3 3 -2 0 -4 -4 0 -2 -4 -2 0 -1 -4 -2 -3 0 0 49 0 0 16 35 0 0 0 0 0 0 0 0 0 0 0 0 0 0.61 0.11 22 S 1 -1 0 -1 -1 0 0 -1 3 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 10 0 0 0 0 0 0 90 0 0 0 0 0.54 0.11 23 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -1 3 5 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 25 75 0 0 0 0.55 0.11 24 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.52 0.11 25 N -2 0 6 2 -3 0 1 -1 3 -4 -4 1 -3 -3 -2 0 -1 -4 -2 -3 0 0 65 8 0 0 10 0 9 0 0 8 0 0 0 0 0 0 0 0 0.63 0.11 26 F -3 -3 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.98 0.11 27 Y -2 -3 -3 -4 -3 -2 -3 -4 1 -1 -1 -3 -1 5 -4 -2 -2 2 7 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 33 0 0 0 0 67 0 0.92 0.11 28 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.52 0.11 29 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.52 0.11 30 N -1 0 5 0 -3 0 0 -1 0 -4 -4 3 -2 -4 -2 1 0 -4 -2 -3 0 0 53 0 0 0 0 0 0 0 0 36 0 0 0 10 0 0 0 0 0.55 0.11 31 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.52 0.11 32 V -1 -3 -4 -4 -1 -3 -3 -4 -4 3 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 10 0 0 0 0 0 0 0 0 0 90 0.51 0.11 33 E -1 0 -1 1 -4 2 6 -3 0 -4 -3 1 -3 -4 -2 0 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.11 34 K -1 3 -1 -1 -4 2 1 -2 -1 -3 -3 5 -2 -4 -2 -1 -1 -3 -2 -3 0 17 0 0 0 10 0 0 0 0 0 73 0 0 0 0 0 0 0 0 0.62 0.11 K Lambda Standard Ungapped 0.1357 0.3212 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1614 0.3195 PSI Gapped 0.0494 0.2670