Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 L -1 -2 -3 -4 -1 -2 -3 -4 -3 1 4 -2 2 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 2 V 0 -2 -3 -3 -1 -2 -2 -3 -3 2 1 -2 1 -1 -2 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 3 P -1 -2 -2 -1 -3 -1 -1 -2 -2 -3 -3 -1 -2 -4 7 -1 -1 -4 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 4 R -1 5 0 -2 -3 1 0 -2 0 -3 -2 2 -1 -3 -2 -1 -1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 5 G 3 -2 -1 -2 -2 -2 -2 5 -2 -3 -3 -2 -2 -3 -2 0 -1 -3 -3 -2 35 0 0 0 0 0 0 65 0 0 0 0 0 0 0 0 0 0 0 0 0.77 0.12 6 S 3 -1 -1 0 -2 0 3 -1 -1 -3 -3 0 -2 -3 -1 2 0 -3 -2 -2 35 0 0 0 0 0 35 0 0 0 0 0 0 0 0 31 0 0 0 0 0.40 0.12 7 H -1 -1 -1 -2 -3 -1 -1 -3 7 0 -1 -2 -1 -2 -3 -2 -1 -3 1 2 0 0 0 0 0 0 0 0 65 0 0 0 0 0 0 0 0 0 0 35 0.62 0.12 8 M -1 0 -2 -2 -3 0 -1 -3 -2 2 0 4 3 -2 -2 -1 -1 -3 -2 0 0 0 0 0 0 0 0 0 0 31 0 53 16 0 0 0 0 0 0 0 0.30 0.14 9 T -1 -2 -1 -2 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.78 0.19 10 I -1 -3 -4 -3 -2 -3 -3 -4 -4 3 1 -3 0 -1 3 -2 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 33 14 0 0 0 21 0 0 0 0 32 0.51 0.19 11 D 1 -1 0 5 -4 0 2 -2 -2 -4 -4 3 -3 -4 -2 -1 -2 -4 -3 -3 14 0 0 45 0 0 11 0 0 0 0 30 0 0 0 0 0 0 0 0 0.62 0.27 12 Q 2 -1 -1 2 -3 2 3 -2 -2 -2 -3 -1 -2 -3 -2 1 -1 -4 -3 1 22 0 0 11 0 15 25 0 0 0 0 0 0 0 0 15 0 0 0 12 0.35 0.30 13 W 0 -2 -2 2 -3 -1 2 -3 -2 1 -2 -2 -2 -1 -3 -2 -2 8 3 -2 12 0 0 12 0 0 23 0 0 14 0 0 0 0 0 0 0 28 11 0 0.68 0.30 14 L 3 -1 -2 -1 -3 0 4 -2 -2 -2 0 1 -1 -3 -2 -1 -1 -4 -3 -2 34 0 0 0 0 0 44 0 0 0 16 7 0 0 0 0 0 0 0 0 0.43 0.31 15 L -2 -3 -5 -5 -2 -3 -4 -5 -4 1 5 -4 1 0 -4 -4 -2 -3 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.94 0.34 16 K 1 -1 -1 -1 -3 3 3 -2 -1 -3 -2 2 -2 0 -2 0 -1 -3 -2 -2 19 0 0 0 0 21 28 0 0 0 5 17 0 6 0 5 0 0 0 0 0.41 0.34 17 N 1 -1 2 1 -3 0 4 -2 -1 -3 -3 2 -3 -3 -2 -1 0 -3 1 -3 19 0 12 8 0 0 34 0 0 0 0 15 0 0 0 0 7 0 6 0 0.41 0.34 18 A 6 -2 -3 -3 -1 -2 -2 -1 -3 -2 -2 -2 -2 -3 -2 0 -1 -4 -3 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.89 0.34 19 K -2 1 -1 -2 -4 0 0 -3 -2 -4 -4 6 -2 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 1.06 0.34 20 E 0 -1 1 3 -4 0 5 -2 -1 -3 -4 1 -3 -4 -2 0 -1 -4 -3 -1 7 0 5 18 0 0 48 0 0 0 0 9 0 0 0 8 0 0 0 5 0.59 0.34 21 D 2 -2 -1 2 -3 1 1 -2 -2 -1 0 1 1 -3 -2 1 -1 -4 -3 0 22 0 0 18 0 5 7 0 0 0 13 10 5 0 0 11 0 0 0 9 0.20 0.34 22 A 6 -2 -3 -3 -1 -2 -2 -1 -3 -2 -2 -2 -2 -3 -2 0 -1 -4 -3 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.89 0.34 23 I -2 -2 0 -3 -3 -2 -3 -4 5 5 1 0 0 -1 -4 -3 -2 -4 -1 1 0 0 5 0 0 0 0 0 19 55 13 8 0 0 0 0 0 0 0 0 0.50 0.34 24 A 2 2 0 -1 -3 2 2 -2 -1 -3 -3 3 -2 -4 -2 1 -1 -4 -3 -3 24 10 5 0 0 10 16 0 0 0 0 27 0 0 0 8 0 0 0 0 0.43 0.34 25 E 1 -1 -1 0 -4 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 13 0 0 0 0 0 87 0 0 0 0 0 0 0 0 0 0 0 0 0 1.00 0.34 26 L 0 -3 -4 -4 -2 -3 -4 -4 -3 0 4 -3 1 3 -4 -3 -2 -2 -1 1 10 0 0 0 0 0 0 0 0 0 66 0 0 14 0 0 0 0 0 11 0.58 0.34 27 K -2 0 2 2 -4 0 0 -2 -2 -3 -3 4 -2 -4 -2 -1 2 -4 -3 -3 0 0 14 16 0 0 0 0 0 0 0 50 0 0 0 0 21 0 0 0 0.58 0.34 28 K 3 -1 -1 0 -3 2 2 -2 -2 -3 -3 3 -2 -4 -2 1 -1 -4 -3 -2 38 0 0 6 0 13 13 0 0 0 0 22 0 0 0 8 0 0 0 0 0.43 0.34 29 A 3 -2 3 1 -2 0 0 -2 -1 -3 -3 -1 -2 -2 -2 0 -1 -2 3 -2 44 0 21 7 0 5 6 0 0 0 0 0 0 0 0 0 0 0 17 0 0.42 0.33 30 G -1 -3 -1 -2 -4 -3 -3 7 -3 -5 -5 -3 -4 -4 -3 -1 -3 -4 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.51 0.33 31 I -2 -3 -3 -3 -2 -3 -3 -4 -3 5 0 -3 0 2 -3 -3 -1 -2 -1 4 1 1 0 1 0 0 1 1 0 46 1 1 0 12 1 1 1 0 0 34 0.55 0.30 32 T 1 -1 -1 -2 -1 -1 -1 -2 -2 -1 -2 0 -1 -2 -1 1 5 -2 -2 -1 11 1 1 1 0 1 1 1 0 1 2 4 0 1 1 4 66 0 1 1 0.56 0.28 33 S 0 -2 0 2 -2 -1 -1 2 -2 -4 -4 -1 -3 -4 -2 5 0 -4 -3 -3 0 0 0 11 0 0 0 18 0 0 0 0 0 0 0 71 0 0 0 0 0.69 0.35 34 D -2 -1 0 5 -4 3 1 -3 -2 -4 -4 3 -3 -4 0 -1 -2 -4 -3 -4 0 0 0 46 0 18 6 0 0 0 0 27 0 0 4 0 0 0 0 0 0.75 0.35 35 F -2 -2 -3 -4 -3 -2 -3 -4 -2 2 2 1 0 4 -4 -3 -2 -1 4 0 0 0 0 0 0 0 0 0 0 21 19 17 0 24 0 0 0 0 16 4 0.44 0.35 36 Y -3 -3 -4 -4 -3 -3 -4 -4 0 -2 1 -3 -1 5 -4 -3 -3 4 7 -2 0 0 0 0 0 0 0 0 0 0 11 0 0 28 0 0 0 5 56 0 1.09 0.35 37 F -2 -1 -2 -3 -3 -2 -2 -4 4 2 1 2 -1 3 -3 -2 -2 -2 0 2 0 0 0 0 0 0 0 0 15 14 11 21 0 20 0 0 0 0 0 19 0.34 0.35 38 N -2 -1 4 5 -4 0 1 -2 -1 -4 -4 3 -3 -4 -2 -1 -1 -5 -3 -4 0 0 25 39 0 0 8 0 0 0 0 24 0 0 0 0 3 0 0 0 0.73 0.35 39 A 2 0 1 -2 -3 1 -2 -3 -2 -1 2 1 -1 -2 -3 -1 -2 5 -2 -2 21 5 11 0 0 9 0 0 0 0 31 12 0 0 0 0 0 11 0 0 0.31 0.35 40 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 1 -4 0 -1 -4 -3 -2 -4 -2 2 0 0 0 0 0 0 0 0 0 91 0 0 0 0 0 0 0 0 0 9 0.92 0.35 41 N -2 -2 6 3 -4 -1 1 -2 -1 -4 -4 -1 -3 -4 -3 0 -1 -5 -3 -4 0 0 65 19 0 0 11 0 0 0 0 0 0 0 0 5 0 0 0 0 0.95 0.35 42 K 3 0 3 -1 -3 -1 -1 -1 -2 -3 -3 4 -2 -4 -2 2 -1 -4 -3 -2 31 0 22 0 0 0 0 0 0 0 0 34 0 0 0 14 0 0 0 0 0.49 0.35 43 A 5 -2 -2 -3 -2 2 -1 0 -2 -3 -3 -1 -2 -3 -2 0 -1 -4 -3 -1 84 0 0 0 0 11 0 5 0 0 0 0 0 0 0 0 0 0 0 0 0.72 0.35 44 K -2 1 2 -1 -4 0 2 -2 -1 -4 -4 6 -3 -4 -2 -1 -2 -4 -3 -3 0 0 14 0 0 0 11 0 0 0 0 74 0 0 0 0 0 0 0 0 0.81 0.35 45 T -1 -2 -1 -2 -2 -2 -2 -3 -3 -2 -2 -2 -2 -3 -2 1 6 -4 -3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 1.02 0.35 46 V -1 -4 -4 -4 -2 -3 -3 -4 -4 2 0 -3 0 -2 2 -3 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 8 0 0 0 0 11 0 0 0 0 80 0.75 0.35 47 E 1 -1 -1 0 -4 3 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 13 0 0 0 0 11 76 0 0 0 0 0 0 0 0 0 0 0 0 0 0.91 0.35 48 E -1 -3 -1 1 -3 -2 -1 6 -3 -5 -5 -2 -4 -4 -3 1 -2 -4 -4 -4 0 0 0 11 0 0 3 75 0 0 0 0 0 0 0 11 0 0 0 0 1.04 0.35 49 V 1 -3 -4 -4 -2 -3 -3 -4 -4 1 1 -3 0 -2 -3 -2 -1 -4 -2 5 11 0 0 0 0 0 0 0 0 0 11 0 0 0 0 0 0 0 0 78 0.66 0.35 50 N -2 0 4 0 -4 2 2 0 -1 -3 -4 3 -3 -4 -2 -1 -1 -4 -3 -2 0 0 35 0 0 7 16 5 0 0 0 32 0 0 0 0 0 0 0 4 0.57 0.35 51 A 4 -2 -1 -2 -2 -1 -1 1 -2 -3 -3 1 -2 -3 -2 3 -1 -4 -3 -2 48 0 0 0 0 0 0 11 0 0 0 9 0 0 0 31 0 0 0 0 0.53 0.35 52 L -2 -3 -4 -5 -2 -3 -4 -5 -2 0 4 -3 1 3 -4 -3 -2 -1 3 1 0 0 0 0 0 0 0 0 0 0 63 0 0 11 0 0 0 0 15 11 0.64 0.35 53 K 1 0 -2 -2 -3 2 -1 -3 -2 -1 -2 5 -2 -3 -2 -1 -1 -4 -3 1 11 0 0 0 0 9 0 0 0 3 0 62 0 0 0 0 0 0 0 15 0.54 0.35 54 N 3 1 3 3 -3 -1 2 -2 -1 -4 -4 -1 -3 -4 -2 0 -1 -4 -3 -3 32 11 20 17 0 0 20 0 0 0 0 0 0 0 0 0 0 0 0 0 0.47 0.35 55 E -2 -1 -1 2 -4 2 6 -3 -1 -4 -2 0 -3 -4 -2 0 -2 -4 -3 -3 0 0 0 10 0 9 73 0 0 0 4 0 0 0 0 4 0 0 0 0 0.88 0.35 56 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 2 -3 0 -1 -3 -2 2 -3 -2 2 0 0 0 0 0 0 0 0 0 55 16 0 0 0 0 0 15 0 0 14 0.60 0.35 57 L -2 -3 -4 -5 -2 -3 -4 -5 -4 4 4 -4 1 -1 -4 -3 -2 -3 -2 2 0 0 0 0 0 0 0 0 0 30 61 0 0 0 0 0 0 0 0 9 0.71 0.35 58 K 1 0 2 -1 -3 0 2 -2 -1 -3 -3 5 -2 -4 -2 0 -1 -4 -3 -3 17 0 10 0 0 0 15 0 0 0 0 53 0 0 0 4 0 0 0 0 0.53 0.31 59 A 4 -2 -1 -2 -1 -1 -1 -1 -2 -3 -3 -1 -2 -3 -2 4 0 -4 -3 -2 59 0 0 0 0 0 0 0 0 0 0 0 0 0 0 41 0 0 0 0 0.65 0.31 60 H -2 1 0 -2 -4 0 -1 -3 9 -3 -1 -1 -2 -2 -3 -2 -2 -3 1 -3 0 10 0 0 0 0 0 0 81 0 9 0 0 0 0 0 0 0 0 0 1.10 0.25 61 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.53 0.09 K Lambda Standard Ungapped 0.1284 0.3139 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1589 0.3181 PSI Gapped 0.0486 0.2670