Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 S 0 -1 3 0 -2 0 0 -1 -1 -3 -3 -1 -2 -3 -1 4 1 -4 -2 -2 0 0 23 0 0 0 0 0 0 0 0 0 0 0 0 77 0 0 0 0 0.52 0.10 2 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.55 0.10 3 V 0 -3 -3 -4 -1 -3 -3 -4 -4 2 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.51 0.10 4 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.60 0.10 5 R 1 6 -1 -2 -3 0 -1 -2 -1 -3 -2 2 -2 -3 -2 -1 -1 -3 -2 -2 23 77 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.64 0.10 6 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.20 0.10 7 F -3 -3 -4 -4 -3 -4 -4 -4 -2 0 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.96 0.10 8 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.60 0.10 9 I -1 -3 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 77 0 0 0 0 0 0 0 0 0 23 0.51 0.10 10 L -1 -2 -2 -3 -2 -2 -2 -3 -3 1 4 -2 2 0 -3 1 -1 -2 -2 0 0 0 0 0 0 0 0 0 0 7 63 0 7 0 0 23 0 0 0 0 0.31 0.10 11 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.20 0.10 12 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.72 0.13 13 E 1 -1 -1 0 -3 1 4 -2 0 -3 -3 0 -2 -1 -2 -1 -1 -1 4 -2 16 0 0 0 0 0 60 0 0 0 0 0 0 0 0 0 0 0 25 0 0.50 0.13 14 D -2 -2 4 6 -4 -1 1 -2 -1 -4 -4 -1 -3 -4 -2 0 -1 -5 -3 -4 0 0 23 77 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.95 0.13 15 R -1 3 -1 -1 -3 0 0 -2 -1 -3 -3 4 -2 -4 4 1 -1 -4 -3 -3 0 16 0 0 0 0 0 0 0 0 0 44 0 0 25 16 0 0 0 0 0.60 0.13 16 R -2 5 0 3 -4 0 0 -2 -1 -4 -3 3 -2 -4 -2 0 -1 -4 -3 -3 0 46 0 25 0 0 0 0 0 0 0 23 0 0 0 6 0 0 0 0 0.56 0.13 17 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -1 1 -3 0 2 -4 -2 -2 1 7 -1 0 0 0 0 0 0 0 0 0 0 25 0 0 0 0 0 0 0 75 0 0.80 0.13 18 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.24 0.13 19 P -1 -3 -1 3 -4 -1 -1 -2 -2 -3 -4 -1 -3 -4 7 -1 -2 -4 -4 -3 0 0 0 25 0 0 0 0 0 0 0 0 0 0 75 0 0 0 0 0 1.59 0.13 20 A 4 -2 -3 -3 -1 -2 -2 -1 -2 0 -1 -2 -1 3 -2 0 -1 -2 0 1 60 0 0 0 0 0 0 0 0 0 0 0 0 25 0 0 0 0 0 16 0.32 0.13 21 L -2 -1 -3 -3 -2 -1 -2 -4 -3 1 4 2 1 -1 -3 -2 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 75 25 0 0 0 0 0 0 0 0 0.39 0.13 22 S 0 -1 0 -1 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 -1 4 4 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 60 40 0 0 0 0.52 0.13 23 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.61 0.13 24 N 1 -1 5 1 -3 0 3 -1 0 -3 -3 0 -2 -4 -2 0 -1 -4 -3 -3 16 0 60 0 0 0 25 0 0 0 0 0 0 0 0 0 0 0 0 0 0.57 0.13 25 E -1 0 -1 1 -4 2 6 -3 0 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.93 0.13 26 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.64 0.13 27 S 0 -1 0 3 -2 0 3 -1 -1 -1 -3 -1 -2 -3 -2 3 0 -4 -2 -2 0 0 0 17 0 0 24 0 0 7 0 0 0 0 0 52 0 0 0 0 0.40 0.13 28 N -2 1 5 0 -3 0 0 -1 0 -4 -4 3 -2 -4 -2 0 -1 -4 -3 -3 0 0 59 0 0 0 0 0 0 0 0 41 0 0 0 0 0 0 0 0 0.64 0.13 29 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 30 A 4 -1 -1 -2 -2 -1 -1 2 -2 -3 -3 2 -2 -3 -2 0 -1 -3 -3 -2 56 0 0 0 0 0 0 19 0 0 0 25 0 0 0 0 0 0 0 0 0.40 0.14 31 K -1 3 -1 -2 -4 0 0 -2 -1 -3 -3 4 -2 -4 4 -1 -1 -4 -3 -3 0 19 0 0 0 0 0 0 0 0 0 56 0 0 25 0 0 0 0 0 0.72 0.14 32 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.24 0.13 33 E -1 0 -1 1 -4 2 6 -3 0 -4 -3 1 -2 -4 -2 0 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.89 0.10 34 K -1 2 -1 -1 -4 1 1 -2 -1 -3 -3 6 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.69 0.10 35 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.55 0.10 36 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -2 0 0 -4 -3 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.92 0.10 37 V -1 -3 -4 -4 -1 -3 -3 -4 -4 3 1 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 24 0 0 0 0 0 0 0 0 0 76 0.49 0.10 38 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.61 0.10 39 I -2 -3 -4 -4 -2 -3 -4 -4 -4 5 3 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 75 25 0 0 0 0 0 0 0 0 0 0.50 0.10 40 G -1 -3 -1 -2 -3 -2 -3 5 -1 -3 -3 -2 -3 0 -3 -1 -2 -1 4 -3 0 0 0 0 0 0 0 73 0 0 0 0 0 0 0 0 0 0 27 0 0.81 0.10 41 Q -1 1 0 -1 -3 6 2 -2 0 -3 -3 1 -1 -4 -2 0 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.83 0.10 42 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.19 0.10 43 D -2 0 1 6 -4 0 1 -2 -1 -4 -4 2 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 73 0 0 0 0 0 0 0 27 0 0 0 0 0 0 0 0 0.78 0.10 44 V -1 -3 -2 -3 -2 -2 -2 -3 -3 1 0 -2 0 -2 4 -1 2 -3 -2 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 28 0 19 0 0 53 0.48 0.09 45 V 0 -3 -3 -4 -1 -3 -3 -4 -4 3 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.48 0.09 46 L -2 -1 -3 -3 -2 -1 -2 -3 -2 1 4 2 1 0 -3 -2 -1 -2 -2 0 0 0 0 0 0 0 0 0 0 0 72 28 0 0 0 0 0 0 0 0 0.32 0.09 47 V 0 -3 -3 -4 -1 -3 -3 -4 -4 3 1 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.48 0.09 48 M -1 -2 -3 -4 -2 -1 -3 -3 -2 1 2 -2 6 0 -3 -2 -1 -2 -1 2 0 0 0 0 0 0 0 0 0 0 0 0 72 0 0 0 0 0 0 28 0.43 0.09 49 K -1 2 0 -1 -4 1 1 -2 -1 -3 -3 6 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.66 0.09 50 R -2 6 -1 -2 -4 1 0 -3 -1 -4 -3 2 -2 -3 -3 -1 -1 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.09 51 K -1 2 0 -1 -4 1 1 -2 -1 -3 -3 6 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.66 0.09 52 R -2 6 -1 -2 -4 1 0 -3 -1 -4 -3 2 -2 -3 -3 -1 -1 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.09 53 D -2 -2 0 6 -4 -1 1 -2 0 -3 -3 -1 -3 0 -2 -1 -2 -1 4 -3 0 0 0 72 0 0 0 0 0 0 0 0 0 0 0 0 0 0 28 0 0.73 0.09 54 S 1 -1 0 -1 -1 0 0 -1 -1 -3 -3 0 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.57 0.09 55 Q -1 1 0 0 -3 6 2 -2 0 -3 -2 1 -1 -4 -1 0 -1 -2 -2 -2 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.70 0.03 56 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.52 0.03 57 A 4 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.45 0.03 58 T -1 -2 -1 -2 -1 -1 -2 -2 -2 2 0 -1 0 -2 -2 1 4 -3 -2 1 0 0 0 0 0 0 0 0 0 31 0 0 0 0 0 0 69 0 0 0 0.32 0.03 59 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.57 0.03 60 R -2 6 -1 -2 -4 1 0 -3 0 -3 -2 2 -2 -3 -2 -1 -1 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.80 0.03 61 I -2 -3 -4 -3 -1 -3 -4 -4 -4 5 2 -3 1 0 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.44 0.03 62 R -2 6 -1 -2 -4 1 0 -3 0 -3 -2 2 -2 -3 -2 -1 -1 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.80 0.03 63 M -1 -2 -3 -4 -2 -1 -3 -3 -2 1 3 -2 6 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 31 0 69 0 0 0 0 0 0 0 0.39 0.03 K Lambda Standard Ungapped 0.1362 0.3187 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1597 0.3172 PSI Gapped 0.0489 0.2670