Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 D -3 -3 0 7 -5 -1 1 -2 -2 -4 -5 -2 -4 -5 2 -1 -2 -5 -4 -4 0 0 0 90 0 0 0 0 0 0 0 0 0 0 10 0 0 0 0 0 1.28 0.30 2 H -2 -1 0 -1 -4 3 3 -3 8 -4 -4 -1 -2 -3 -3 1 -2 -3 0 -4 0 0 0 0 0 15 20 0 55 0 0 0 0 0 0 10 0 0 0 0 0.91 0.30 3 Y -3 -3 -3 -4 -3 -3 -3 -4 1 -2 -2 -3 -2 3 -4 -3 -2 1 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 86 9 1.26 0.30 4 N -2 1 2 -1 -3 1 2 -3 1 2 -1 2 1 -3 -3 -1 0 -4 -2 0 0 8 12 0 0 6 14 0 4 20 4 16 4 0 0 0 6 0 0 7 0.20 0.30 5 C -1 -5 -4 -5 11 -4 -5 -4 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -3 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.01 0.30 6 V 3 -3 -3 -3 -2 -2 -3 -2 -3 1 -1 -2 -1 -2 -3 1 -1 -4 -2 4 36 0 0 0 0 0 0 0 0 6 0 0 0 0 0 9 0 0 0 48 0.44 0.30 7 S -2 4 0 0 -4 0 1 0 -1 -4 -3 4 -3 -3 -2 1 0 -3 0 -3 0 28 4 4 0 0 9 7 0 0 0 30 0 0 0 9 4 0 4 0 0.44 0.30 8 S -2 2 5 -1 -4 1 -1 -1 -1 -4 -4 4 -3 -4 -2 1 -1 -4 -3 -4 0 9 36 0 0 6 0 3 0 0 0 35 0 0 0 12 0 0 0 0 0.58 0.30 9 G -1 -3 -1 -2 -4 -2 -1 6 -3 -5 -5 0 -4 -4 -3 -1 -2 -4 -4 -4 0 0 0 0 0 0 4 90 0 0 0 6 0 0 0 0 0 0 0 0 1.24 0.30 10 G -1 -3 -1 -2 -4 -3 -3 7 -3 -5 -5 -3 -4 -4 -3 -1 -3 -4 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.48 0.30 11 Q -1 -2 -1 -2 -2 2 -1 -3 3 -1 -2 -2 -1 3 -3 0 5 -3 -1 -1 0 0 0 0 0 12 0 0 9 4 0 0 0 16 0 0 58 0 0 0 0.51 0.30 12 C -1 -4 -4 -5 10 -4 -5 -4 -3 -2 -2 -4 -2 -2 -4 -2 -2 -3 1 -2 0 0 0 0 93 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 1.81 0.30 13 L -3 -2 6 -1 -3 -1 -2 -2 3 -2 2 -2 -1 -2 -3 -1 -1 -4 -2 -2 0 0 59 0 0 0 0 0 9 0 32 0 0 0 0 0 0 0 0 0 0.63 0.30 14 Y -3 -3 -4 -4 -3 -3 -4 -4 0 -1 1 -3 -1 5 -4 -3 -3 1 7 0 0 0 0 0 0 0 0 0 0 0 10 0 0 31 0 0 0 0 50 9 0.91 0.30 15 S 0 -2 0 -1 -2 -1 -1 -1 -1 -3 -3 -1 -2 -2 -2 6 0 -3 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 91 0 0 9 0 0.75 0.30 16 A 1 -1 2 -2 -3 0 -2 -2 0 -3 -2 -1 -2 -4 6 1 -1 -4 -3 -3 18 4 15 0 0 3 0 0 3 0 4 0 0 0 40 13 0 0 0 0 0.73 0.30 17 C -1 -5 -4 -5 11 -4 -5 -4 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -3 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.01 0.30 18 P -2 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -4 -5 8 -2 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.39 0.30 19 I -2 1 1 -3 -2 -2 -3 -4 -3 2 3 -2 3 -1 -3 0 -1 -3 -2 1 0 9 9 0 0 0 0 0 0 9 45 0 9 0 0 8 0 0 0 10 0.33 0.30 20 F -3 -3 0 -4 -4 -3 -3 -4 0 -2 -1 -3 -1 6 -4 -3 -3 1 6 -2 0 0 8 0 0 0 0 0 0 0 0 0 0 53 0 0 0 0 40 0 1.03 0.30 21 T -1 -2 3 -1 -2 -1 -1 -2 -2 -3 -3 -1 -2 -3 -2 3 4 -4 -3 -2 0 0 21 0 0 0 0 0 0 0 0 0 0 0 0 35 44 0 0 0 0.62 0.30 22 K -2 5 -1 -2 -4 2 1 -3 1 -4 -3 5 -2 -4 -2 -1 -2 -4 -3 -3 0 33 0 0 0 6 4 0 4 0 0 54 0 0 0 0 0 0 0 0 0.78 0.30 23 I -2 -3 -3 -4 -2 -3 -3 -4 3 5 1 -3 1 -1 -4 -3 -1 -3 2 3 0 0 0 0 0 0 0 0 10 52 4 0 4 0 0 0 3 0 9 17 0.51 0.30 24 Q -2 -1 -1 2 -4 3 5 -3 -1 0 -3 1 -2 -4 -2 -1 0 -4 -3 -1 0 0 0 11 0 15 46 0 0 9 0 9 0 0 0 0 4 0 0 6 0.51 0.30 25 G -1 -3 -1 -2 -4 -3 -3 7 -3 -5 -5 -3 -4 -4 -3 -1 -3 -4 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.48 0.30 26 T 0 -2 -1 -2 -2 -2 -2 -3 -2 -2 -2 -2 -2 -2 -2 1 6 -3 1 -1 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 90 0 6 0 0.82 0.30 27 C -1 -5 -4 -5 11 -4 -5 -4 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -3 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.01 0.30 28 Y -2 -3 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 3 -4 -1 -2 1 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 9 0 7 0 0 84 0 1.24 0.30 29 R -1 3 4 -1 -4 1 -1 3 1 -4 -4 1 -3 -4 -3 1 -1 -4 -3 -4 0 22 27 0 0 5 0 21 4 0 0 9 0 0 0 12 0 0 0 0 0.49 0.30 30 G -1 -3 -1 -2 -4 -3 -3 7 -3 -5 -5 -1 -4 -4 -3 -1 -3 -4 -4 -4 0 0 0 0 0 0 0 96 0 0 0 4 0 0 0 0 0 0 0 0 1.37 0.30 31 E -2 4 -1 -2 -4 1 1 -3 -1 -4 -3 6 -2 -4 -2 -1 -2 -4 -3 -3 0 25 0 0 0 0 4 0 0 0 0 71 0 0 0 0 0 0 0 0 0.87 0.30 32 A 5 -2 -2 0 -1 -2 -2 -1 -3 -2 -3 -2 -2 -3 -2 0 -1 -4 -3 -1 93 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.75 0.30 33 K -2 1 1 -1 -4 0 0 -2 -2 -4 -4 6 -2 -4 -2 -1 -2 -4 -3 -3 0 0 6 0 0 0 0 0 0 0 0 94 0 0 0 0 0 0 0 0 0.93 0.30 34 C -1 -5 -4 -5 11 -4 -5 -4 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -3 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.01 0.30 35 C -1 -5 -4 -5 10 -4 -5 -4 -4 -2 -2 -4 -2 -3 -4 -2 -2 -3 -3 -2 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.96 0.29 36 K -1 2 -1 -1 -4 1 1 -2 -1 -3 -3 6 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.68 0.10 K Lambda Standard Ungapped 0.1394 0.3259 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1705 0.3102 PSI Gapped 0.0522 0.2670