Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 P -1 -2 -2 -1 -3 -1 -1 -2 -2 -3 -3 -1 -2 -4 7 -1 -1 -4 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 2 D -2 -2 1 6 -3 0 1 -1 -1 -3 -4 -1 -3 -3 -1 0 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 3 K -1 2 0 -1 -3 1 1 -1 -1 -3 -2 4 -1 -3 -1 0 -1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 4 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.59 0.07 5 Y -2 -2 -2 -3 -3 -2 -2 -3 0 -2 -2 -2 -2 1 6 -2 -2 0 5 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 50 0 0 0 50 0 1.08 0.07 6 E -1 0 0 1 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.07 7 E -1 0 0 1 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.07 8 M -1 -2 -3 -4 -2 -1 -3 -4 -2 1 3 -2 5 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 50 0 50 0 0 0 0 0 0 0 0.46 0.07 9 V 3 -2 -2 -3 -1 -2 -2 -1 -3 1 0 -2 0 -2 -2 0 0 -3 -2 3 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 50 0.29 0.07 10 K -1 1 0 0 -4 1 4 -2 -1 -3 -3 4 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 50 0 0 0 0 50 0 0 0 0 0 0 0 0 0.58 0.07 11 E -1 0 0 1 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.07 12 V -1 -3 -4 -4 -1 -3 -3 -4 -3 2 3 -3 1 0 -3 -2 -1 -3 -1 3 0 0 0 0 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 50 0.40 0.07 13 E -1 -1 -2 0 -2 0 4 -3 -2 1 -1 -1 -1 -2 -2 -1 -1 -3 -2 3 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 0 0 0 0 50 0.31 0.07 14 R -1 5 -1 -1 -4 1 0 -2 -1 -3 -3 4 -2 -3 -2 -1 -1 -3 -2 -3 0 50 0 0 0 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 0.65 0.07 15 L -2 -3 -4 -4 -2 -2 -3 -4 -3 1 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.56 0.07 16 K -1 5 -1 -1 -4 1 0 -2 -1 -3 -3 4 -2 -3 -2 -1 -1 -3 -2 -3 0 50 0 0 0 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 0.65 0.07 17 L -1 -2 -3 -4 -2 -1 -3 -4 -2 1 3 -2 5 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 50 0 50 0 0 0 0 0 0 0 0.46 0.07 18 E -1 0 0 1 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.07 19 N -2 -1 6 1 -3 0 0 -1 0 -4 -4 0 -3 -3 -2 0 0 -4 -2 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.86 0.07 20 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.63 0.07 21 T 3 -2 -1 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -3 -1 1 4 -3 -2 0 50 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 50 0 0 0 0.39 0.07 22 L -2 -3 -4 -4 -2 -2 -3 -4 -3 1 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.56 0.07 23 K -1 4 -1 -1 -4 1 0 -2 -1 -3 -3 5 -2 -4 -2 -1 -1 -3 -2 -3 0 32 0 0 0 0 0 0 0 0 0 68 0 0 0 0 0 0 0 0 0.68 0.12 24 Q 0 0 0 -1 -2 4 0 -1 -1 -3 -3 0 -2 -3 -1 4 1 -3 -2 -2 0 0 0 0 0 33 0 0 0 0 0 0 0 0 0 67 0 0 0 0 0.51 0.12 25 K -1 1 -1 -1 -3 0 0 3 -2 -4 -3 5 -2 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 0 32 0 0 0 68 0 0 0 0 0 0 0 0 0.57 0.12 26 V 0 -2 -2 -2 -1 -2 -2 -2 -3 1 2 -2 0 -1 -2 2 0 -3 -2 2 0 0 0 0 0 0 0 0 0 0 35 0 0 0 0 32 0 0 0 33 0.24 0.12 27 D -3 -2 0 7 -5 -1 2 -2 -2 -4 -5 -1 -4 -5 -2 -1 -2 -5 -4 -4 0 0 0 90 0 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 1.32 0.31 28 S -1 3 -1 -1 -3 0 3 -3 -2 -2 -3 0 -2 -4 3 1 1 -4 -3 0 0 19 0 0 0 0 29 0 0 0 0 0 0 0 16 14 10 0 0 12 0.43 0.31 29 I -2 -4 -4 -4 -2 -3 -3 -4 -4 4 1 -3 2 -2 3 -3 -1 -4 -2 4 0 0 0 0 0 0 0 0 0 33 8 0 6 0 17 0 0 0 0 37 0.61 0.35 30 L -2 -3 -5 -5 -2 -3 -4 -5 -4 2 5 -4 1 -1 -4 -4 -2 -3 -2 2 0 0 0 0 0 0 0 0 0 13 74 0 0 0 0 0 0 0 0 13 0.78 0.40 31 T -1 -2 2 -2 -2 -2 -2 -2 -2 0 -2 -2 -2 -3 -2 2 5 -4 -3 -1 0 0 11 0 0 0 0 0 0 5 0 0 0 0 0 20 63 0 0 0 0.70 0.40 32 A 3 -2 0 -2 -3 1 -2 -2 -2 -3 -3 -2 -3 -4 6 1 -1 -4 -3 -3 31 0 6 0 0 9 0 0 0 0 0 0 0 0 46 8 0 0 0 0 0.98 0.40 33 A 3 4 -2 -2 -2 -1 -2 -2 -2 -3 -2 -1 2 -3 -2 2 1 -4 -3 -2 36 29 0 0 0 0 0 0 0 0 0 0 7 0 0 22 7 0 0 0 0.49 0.41 34 K 0 0 -1 -1 -4 6 3 -3 -1 -4 -3 2 -2 -4 -2 -1 -2 -4 -3 -3 9 0 0 0 0 56 19 0 0 0 0 16 0 0 0 0 0 0 0 0 0.84 0.41 35 R -2 4 -1 -2 -4 0 0 -3 -2 -4 -4 6 -3 -4 -2 -1 -2 -4 -3 -4 0 26 0 0 0 0 0 0 0 0 0 74 0 0 0 0 0 0 0 0 1.02 0.41 36 E -2 -1 -1 3 -5 3 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -4 0 0 0 12 0 18 70 0 0 0 0 0 0 0 0 0 0 0 0 0 1.03 0.41 37 S 4 -3 -2 -3 -2 -2 -2 -1 -3 -1 -2 -2 -2 -3 -2 2 1 -4 -3 2 56 0 0 0 0 0 0 0 0 0 0 0 0 0 0 21 6 0 0 18 0.55 0.41 38 I -2 0 -3 -4 -3 1 -3 -4 -3 3 2 0 6 -1 -4 -3 -2 -3 -2 0 0 7 0 0 0 7 0 0 0 26 23 6 31 0 0 0 0 0 0 0 0.53 0.41 39 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 2 -4 0 -1 -4 -4 -2 -4 -2 2 0 0 0 0 0 0 0 0 0 78 13 0 0 0 0 0 0 0 0 9 0.89 0.41 40 V 0 -2 3 -2 -2 -2 -2 1 -2 -1 -1 -2 -2 -3 -3 1 3 -4 -3 1 6 0 17 0 0 0 0 14 0 0 6 0 0 0 0 11 31 0 0 17 0.35 0.41 41 S 3 -2 -1 -2 -2 3 -1 -1 -2 -3 -3 -1 -2 -4 -2 4 0 -4 -3 -2 30 0 0 0 0 18 0 0 0 0 0 0 0 0 0 52 0 0 0 0 0.63 0.41 42 S 4 -3 -2 -3 -2 -2 -2 -2 -2 -1 -2 -2 -2 -2 -2 1 2 -3 2 2 42 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 13 0 11 25 0.42 0.41 43 S -1 -3 -3 -4 2 -3 -3 -4 -4 2 1 -3 -1 -2 -3 0 2 -4 -3 4 0 0 0 0 6 0 0 0 0 7 11 0 0 0 0 10 16 0 0 50 0.49 0.41 44 R 0 2 4 -1 -3 -1 -2 2 -2 -4 -4 -1 -3 -4 -3 3 1 -4 -3 -3 5 16 23 0 0 0 0 18 0 0 0 0 0 0 0 28 11 0 0 0 0.49 0.41 45 A 3 3 -2 -2 -3 -1 -1 1 -2 -3 -3 3 -2 -4 -2 2 -1 -4 -3 -3 34 18 0 0 0 0 0 9 0 0 0 23 0 0 0 16 0 0 0 0 0.47 0.41 46 L -3 -3 -5 -5 -3 -3 -4 -5 -4 0 5 -4 1 2 -4 -4 -2 -2 -1 0 0 0 0 0 0 0 0 0 0 0 89 0 0 11 0 0 0 0 0 0 0.91 0.41 47 G 0 -2 -1 -2 2 -2 -2 1 -3 -2 -3 -2 -2 -3 -2 4 4 -4 -3 0 5 0 0 0 6 0 0 10 0 0 0 0 0 0 0 37 37 0 0 6 0.53 0.41 48 A 2 1 1 -2 -3 -1 -1 -2 -2 -3 -3 3 -2 -4 -2 4 -1 -4 -3 -3 23 9 6 0 0 0 0 0 0 0 0 23 0 0 0 40 0 0 0 0 0.50 0.41 49 V 1 -1 1 -2 -3 3 -1 -3 -2 -1 2 0 -1 -3 -3 0 -1 -4 -3 1 13 0 10 0 0 23 0 0 0 0 23 6 0 0 0 9 0 0 0 15 0.25 0.41 50 A 6 -3 -3 -3 -1 -2 -2 -1 -3 -2 -3 -2 -2 -3 -2 0 -1 -4 -3 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.97 0.41 51 M 1 -2 -2 0 -3 -1 2 -2 -2 -2 -1 -2 5 -3 -2 2 2 -4 -3 -2 15 0 0 6 0 0 17 0 0 0 0 0 28 0 0 18 16 0 0 0 0.40 0.41 52 R -2 3 -1 -1 -4 3 2 -3 -2 -4 -4 5 -2 -4 -2 -1 0 -4 -3 -3 0 15 0 0 0 13 12 0 0 0 0 53 0 0 0 0 6 0 0 0 0.74 0.41 53 K -2 1 -1 -2 -4 2 0 -3 -2 -4 -4 6 -2 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 9 0 0 0 0 0 91 0 0 0 0 0 0 0 0 1.06 0.41 54 I -2 -4 -5 -4 -2 -4 -4 -5 -4 6 2 -4 0 -1 -4 -4 -2 -4 -2 2 0 0 0 0 0 0 0 0 0 89 11 0 0 0 0 0 0 0 0 0 0.98 0.41 55 E -2 3 -1 0 -5 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -4 0 23 0 0 0 0 77 0 0 0 0 0 0 0 0 0 0 0 0 0 1.06 0.41 56 A 2 -2 -2 2 -3 1 2 -2 -2 0 0 -2 -2 2 -3 1 -1 -3 -2 -2 27 0 0 11 0 7 15 0 0 6 10 0 0 11 0 13 0 0 0 0 0.24 0.41 57 K -2 5 -1 -2 -4 0 -1 -3 -2 -4 -4 5 -3 -4 -3 -2 -2 -4 -3 -4 0 46 0 0 0 0 0 0 0 0 0 54 0 0 0 0 0 0 0 0 1.02 0.41 58 V -1 -4 -4 -4 -2 -3 -4 -5 -4 3 1 -4 0 -2 -4 -3 -1 -4 -2 6 0 0 0 0 0 0 0 0 0 9 11 0 0 0 0 0 0 0 0 81 0.83 0.41 59 R 0 6 -2 -3 -3 -1 -2 -3 -2 -2 -1 0 -2 -3 -3 1 0 -4 -3 0 11 60 0 0 0 0 0 0 0 0 6 0 0 0 0 10 6 0 0 7 0.64 0.41 60 S 2 -1 2 -2 -3 -1 -1 -2 2 -3 -3 4 -2 -4 -2 1 3 -4 -3 -2 19 0 10 0 0 0 0 0 5 0 0 32 0 0 0 10 23 0 0 0 0.44 0.41 61 R 0 1 -1 3 3 -1 2 -3 1 0 0 0 -2 -3 -3 0 -2 -4 -3 0 6 10 0 23 7 0 16 0 5 7 11 6 0 0 0 5 0 0 0 6 0.25 0.41 62 A 0 -3 -3 -3 -2 -2 -3 -3 -3 1 1 -3 2 1 -3 -1 4 -3 -2 2 10 0 0 0 0 0 0 0 0 7 16 0 7 7 0 0 36 0 0 18 0.39 0.41 63 A 3 -2 -2 1 -2 -1 2 -2 -3 -1 2 -2 -1 -2 -3 1 -1 -4 -3 -1 32 0 0 11 0 0 14 0 0 0 31 0 0 0 0 12 0 0 0 0 0.32 0.41 64 K -2 1 -2 1 -4 -1 -1 1 -2 -4 -4 4 -3 -5 5 -2 -2 -4 -4 -4 0 5 0 7 0 0 0 11 0 0 0 44 0 0 33 0 0 0 0 0 0.85 0.41 65 A 3 -3 -4 -4 3 -3 -3 -3 -4 1 3 -3 0 -2 -3 -2 -2 -3 -3 3 29 0 0 0 7 0 0 0 0 6 33 0 0 0 0 0 0 0 0 25 0.45 0.41 66 V -2 0 0 -3 -2 1 -2 -4 -3 0 -1 -2 -1 1 -3 -2 2 -4 -2 4 0 7 6 0 0 11 0 0 0 0 0 0 0 6 0 0 14 0 0 56 0.42 0.41 67 T -1 -2 -1 -2 -2 -2 -2 -3 -3 -2 -2 -2 -2 -3 -2 1 6 -4 -3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 94 0 0 0 1.04 0.41 68 E -2 4 -1 -2 -4 2 2 -3 -2 -3 -3 5 -2 -4 -3 -1 -2 -4 -3 -1 0 24 0 0 0 7 16 0 0 0 0 47 0 0 0 0 0 0 0 7 0.69 0.41 69 Q -2 -1 3 1 -4 3 2 0 2 -4 -4 2 -3 -4 1 0 -2 -4 -3 -4 0 0 20 7 0 21 16 6 6 0 0 12 0 0 6 7 0 0 0 0 0.48 0.41 70 E -2 -1 -1 0 -5 5 5 -3 -1 -4 -4 0 -2 -4 -2 -1 -2 -4 -3 -4 0 0 0 0 0 42 58 0 0 0 0 0 0 0 0 0 0 0 0 0 1.06 0.41 71 L 0 -4 -4 -4 6 -3 -4 -4 -4 2 3 -4 3 2 -4 -3 -2 -3 -2 1 7 0 0 0 23 0 0 0 0 11 28 0 11 9 0 0 0 0 0 12 0.56 0.41 72 T -2 -2 -2 1 -4 0 5 -3 -2 -2 -2 -1 4 -3 -3 -1 1 -4 -3 1 0 0 0 7 0 0 55 0 0 0 0 0 18 0 0 0 10 0 0 11 0.61 0.41 73 S 1 -2 2 4 -4 1 4 -2 -2 -4 -4 -1 -3 -4 -2 1 -1 -4 -3 -3 16 0 12 23 0 7 32 0 0 0 0 0 0 0 0 10 0 0 0 0 0.58 0.41 74 L 4 -2 -3 -3 -2 -2 -2 -2 -3 2 0 0 -1 -2 -3 -1 -1 -4 -3 2 53 0 0 0 0 0 0 0 0 11 10 7 0 0 0 0 0 0 0 19 0.44 0.41 75 L -3 -4 -5 -5 -2 -4 -4 -5 -4 5 4 -4 1 -1 -4 -4 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 44 56 0 0 0 0 0 0 0 0 0 0.81 0.41 76 Q 2 0 -1 -2 -3 4 2 -2 -2 -3 -3 3 -2 -4 -2 1 0 -4 -3 -3 27 0 0 0 0 22 11 0 0 0 0 27 0 0 0 7 6 0 0 0 0.48 0.41 77 S -1 -2 5 -1 -3 -2 -2 3 2 -1 -4 -2 -3 -4 -3 2 1 -4 -3 -3 0 0 34 0 0 0 0 29 6 6 0 0 0 0 0 17 9 0 0 0 0.57 0.41 78 L -2 -3 -4 -4 -2 -3 -4 -5 -4 4 4 -3 1 -1 -4 -3 1 -3 -2 1 0 0 0 0 0 0 0 0 0 27 56 0 0 0 0 0 11 0 0 6 0.65 0.41 79 T -1 2 1 -2 -3 -1 -1 -3 2 -3 -3 3 -2 -4 -2 2 4 -4 -2 -2 0 11 6 0 0 0 0 0 7 0 0 23 0 0 0 15 38 0 0 0 0.51 0.41 80 L -3 -3 -4 -5 -3 -3 -4 -5 -2 3 3 -4 2 2 -4 -3 -2 -1 4 1 0 0 0 0 0 0 0 0 0 28 27 0 7 9 0 0 0 0 23 6 0.60 0.41 81 R -3 7 -2 -3 -5 0 -1 -4 -1 -4 -3 1 -3 -4 -3 -2 -2 -4 -3 -4 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.42 0.41 82 V -2 -4 -4 -4 -2 -4 -4 -5 -4 5 2 -4 0 -1 -4 -3 -2 -4 -2 4 0 0 0 0 0 0 0 0 0 48 17 0 0 0 0 0 0 0 0 35 0.76 0.41 83 D -2 -2 0 5 -4 3 2 -3 3 -4 -4 -1 -3 -4 -3 1 -2 -4 -3 -4 0 0 0 52 0 18 10 0 11 0 0 0 0 0 0 9 0 0 0 0 0.85 0.41 84 V -2 -4 -4 -5 -2 -3 -4 -5 -4 1 3 -4 0 -1 -4 -3 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 0 31 0 0 0 0 0 0 0 0 69 0.76 0.41 85 S 0 -2 -1 -2 3 -1 -2 -2 -2 -3 -3 -2 -2 0 -2 5 2 -4 -2 -2 0 0 0 0 7 0 0 0 0 0 0 0 0 6 0 77 11 0 0 0 0.74 0.41 86 M -2 -3 -3 -4 -3 -2 -3 -4 -3 1 2 -3 7 -1 2 -2 0 -2 2 -1 0 0 0 0 0 0 0 0 0 6 17 0 51 0 10 0 6 0 9 0 0.68 0.41 87 E 1 -2 -1 3 -3 2 4 -2 -2 -4 -4 -1 -3 -4 -2 3 -1 -4 -3 -3 9 0 0 18 0 11 32 0 0 0 0 0 0 0 0 29 0 0 0 0 0.60 0.41 88 E -2 -2 0 5 -5 0 5 -3 -1 -4 -4 -1 -3 -4 -2 -1 -2 -4 -3 -4 0 0 0 38 0 0 62 0 0 0 0 0 0 0 0 0 0 0 0 0 1.05 0.35 89 L -2 -3 -4 -4 -2 -3 -3 -4 -4 1 4 -3 1 0 -3 -3 -1 -2 -2 2 0 0 0 0 0 0 0 0 0 0 78 0 0 0 0 0 0 0 0 22 0.58 0.18 90 E -1 0 0 1 -4 2 5 -2 0 -3 -3 1 -2 -3 -1 0 -1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 91 H -2 0 1 -1 -3 0 0 -2 7 -3 -3 -1 -2 -1 -2 -1 -2 -2 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 92 H -2 0 1 -1 -3 0 0 -2 7 -3 -3 -1 -2 -1 -2 -1 -2 -2 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 K Lambda Standard Ungapped 0.1219 0.3090 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1630 0.3162 PSI Gapped 0.0499 0.2670