Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.43 0.02 2 T 1 -1 0 -1 -1 -1 -1 -1 -2 -1 -1 -1 -1 -2 -1 1 4 -3 -2 0 19 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 81 0 0 0 0.45 0.05 3 F -2 -3 -3 -4 -3 -3 -3 -3 -2 0 0 -3 0 7 -4 -3 -2 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.94 0.09 4 G 1 2 0 3 -2 -1 0 3 -1 -3 -3 0 -2 -3 -2 1 -1 -3 -3 -2 15 20 0 23 0 0 0 28 0 0 0 0 0 0 0 14 0 0 0 0 0.36 0.09 5 M -1 -1 -2 -2 -2 0 2 -3 -2 0 2 -1 5 -1 -2 -2 -1 -2 -1 0 0 0 0 0 0 0 23 0 0 0 29 0 48 0 0 0 0 0 0 0 0.32 0.09 6 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.18 0.09 7 D -2 -2 1 6 -4 -1 1 -2 -1 -3 -4 -1 -3 -4 -2 -1 -1 -5 -3 -3 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.09 0.09 8 R -2 5 -1 -2 -3 0 -1 -3 -1 -1 1 2 0 -2 -2 -1 -1 -3 -2 -2 0 57 0 0 0 0 0 0 0 0 23 20 0 0 0 0 0 0 0 0 0.47 0.09 9 V 0 -3 -3 -3 -1 -2 -3 -3 -3 2 1 -3 0 -1 -3 -2 0 -3 -1 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.49 0.09 10 R -2 6 -1 -2 -4 1 0 -3 -1 -3 -2 2 -2 -3 -2 -1 -1 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.91 0.09 11 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.66 0.09 12 K -1 1 -1 -1 -3 1 0 -2 -1 -1 -2 5 -1 -3 -2 -1 -1 -3 -2 1 0 0 0 0 0 0 0 0 0 0 0 80 0 0 0 0 0 0 0 20 0.43 0.09 13 S 1 2 0 -1 -2 0 0 -1 -1 -3 -3 0 -2 -3 -1 4 1 -3 -2 -2 0 20 0 0 0 0 0 0 0 0 0 0 0 0 0 80 0 0 0 0 0.47 0.09 14 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.18 0.09 15 A 3 -1 -1 -1 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 3 0 -3 -2 -1 57 0 0 0 0 0 0 0 0 0 0 0 0 0 0 43 0 0 0 0 0.44 0.09 16 A 3 -1 -2 -2 -1 2 -1 -1 -1 -2 -2 -1 -1 -2 -2 0 -1 7 0 -1 57 0 0 0 0 20 0 0 0 0 0 0 0 0 0 0 0 23 0 0 0.55 0.09 17 W -3 -3 -4 -5 -3 -2 -3 -3 -3 -3 -2 -3 -2 1 -4 -3 -3 11 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.54 0.09 18 Q -1 0 0 0 -3 5 3 -2 4 -3 -3 1 -1 -3 -2 -1 -1 -2 -1 -3 0 0 0 0 0 57 23 0 20 0 0 0 0 0 0 0 0 0 0 0 0.63 0.09 19 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.18 0.09 20 Q -1 5 -1 -1 -3 4 1 -2 0 -3 -2 2 -1 -3 -2 -1 -1 -3 -2 -3 0 58 0 0 0 42 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.69 0.09 21 I -1 -3 -3 -3 -1 -3 -3 -4 -3 4 1 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 43 0 0 0 0 0 0 0 0 0 57 0.47 0.09 22 V 0 -3 -3 -3 -1 -2 -3 -3 -3 2 1 -3 0 -1 -3 -2 0 -3 -1 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.49 0.09 23 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.18 0.09 24 W -2 -3 -3 -3 -2 -2 -3 -3 -2 -2 -2 -3 -2 0 -3 -1 1 10 1 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 23 77 0 0 1.78 0.09 25 Y -2 -2 -2 -3 -3 -2 -2 -3 1 -2 -1 -2 -1 3 -3 -2 -2 2 7 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.06 0.09 26 C 0 -2 -1 -2 8 -2 -2 -2 -2 -2 -2 -2 -2 -3 -2 1 2 -3 -2 -1 0 0 0 0 57 0 0 0 0 0 0 0 0 0 0 20 23 0 0 0 0.75 0.09 27 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.62 0.09 28 N -1 0 2 3 -3 1 2 -2 -1 -3 -3 2 -2 -3 -2 0 1 -3 -2 -2 0 0 14 20 0 0 23 0 0 0 0 28 0 0 0 0 15 0 0 0 0.40 0.09 29 L -2 -1 -2 -3 -2 2 -1 -3 -2 1 4 -1 1 0 -3 -2 -1 -2 -1 0 0 0 0 0 0 23 0 0 0 0 77 0 0 0 0 0 0 0 0 0 0.38 0.09 30 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.62 0.09 31 P -1 -2 -2 -2 -3 -2 -1 -2 -2 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.15 0.09 32 E -1 3 -1 1 -4 1 5 -2 0 -3 -3 1 -2 -3 -2 -1 -1 -3 -2 -3 0 23 0 0 0 0 77 0 0 0 0 0 0 0 0 0 0 0 0 0 0.70 0.09 33 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.18 0.09 34 Y -2 -2 -2 -3 -3 -2 -2 -3 1 -2 -1 -2 -1 3 -3 -2 -2 2 7 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.06 0.09 35 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.53 0.09 36 V 0 -3 -3 -3 -1 -2 -3 -3 -3 2 1 -3 0 -1 -3 -2 0 -3 -1 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.49 0.09 37 E -1 0 0 1 -4 3 5 -2 0 -3 -3 1 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 23 77 0 0 0 0 0 0 0 0 0 0 0 0 0 0.76 0.09 38 S 0 -1 0 -1 -1 -1 -1 -1 -2 -1 1 -1 0 -2 -1 4 1 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 23 0 0 0 0 77 0 0 0 0 0.34 0.09 39 E -1 -1 0 4 -4 1 4 -2 -1 -3 -3 0 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 43 0 0 57 0 0 0 0 0 0 0 0 0 0 0 0 0 0.78 0.09 40 A 2 0 0 -1 -1 0 0 -1 -1 -2 -2 2 -1 -3 -1 2 2 -3 -2 -1 28 0 0 0 0 0 0 0 0 0 0 23 0 0 0 29 20 0 0 0 0.31 0.09 41 H -2 -1 0 -1 -3 0 2 -2 7 -3 -3 -1 -2 -2 4 -1 -2 -3 0 -3 0 0 0 0 0 0 20 0 57 0 0 0 0 0 23 0 0 0 0 0 0.79 0.09 42 P -1 2 -2 -2 -3 -1 -2 -3 -2 -2 -2 -1 -2 3 6 -1 -2 -2 0 -2 0 20 0 0 0 0 0 0 0 0 0 0 0 23 57 0 0 0 0 0 1.02 0.09 43 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 -1 -2 -3 -3 -3 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.18 0.09 44 S 1 -1 0 -1 -1 -1 -1 -1 -1 -3 -3 -1 -2 -3 4 4 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 23 77 0 0 0 0 0.63 0.09 45 V 0 -3 -3 -3 -1 -2 -3 -3 -3 2 1 -3 0 -1 -3 -2 0 -3 -1 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.49 0.09 46 Q -1 1 0 -1 -3 6 2 -2 0 -3 -2 1 -1 -3 -2 0 -1 -2 -2 -2 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.81 0.09 47 I -2 -3 -4 -3 -2 -3 -3 -4 -4 5 1 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.55 0.09 48 Y -2 -2 -2 -3 -3 -2 -2 -3 1 -2 -1 -2 -1 3 -3 -2 -2 2 7 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.06 0.09 49 P -1 -2 -2 -2 -3 -2 -1 -2 -2 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.15 0.09 50 V 1 -2 -2 -1 -2 -1 2 -2 -2 1 0 -1 2 -2 -2 -1 -1 -3 -2 3 20 0 0 0 0 0 23 0 0 0 0 0 15 0 0 0 0 0 0 42 0.19 0.09 51 A 3 -1 0 -1 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 3 2 -3 -2 -1 42 0 0 0 0 0 0 0 0 0 0 0 0 0 0 43 15 0 0 0 0.40 0.09 52 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.53 0.09 53 L -2 -2 -4 -4 -2 -2 -3 -4 -3 1 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.59 0.09 54 E -1 0 -1 1 -4 2 5 -2 0 -3 -3 1 -2 -3 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.87 0.09 55 R -2 6 -1 -2 -4 1 0 -2 0 -3 -2 2 -2 -3 -2 -1 -1 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.05 56 I -1 -3 -3 -3 -1 -3 -3 -4 -3 4 1 -3 1 -1 -3 -2 0 -3 -1 3 0 0 0 0 0 0 0 0 0 49 0 0 0 0 0 0 0 0 0 51 0.38 0.02 57 N -2 0 6 1 -3 0 0 0 1 -3 -3 0 -2 -3 -2 1 0 -4 -2 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.73 0.01 K Lambda Standard Ungapped 0.1329 0.3163 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1412 0.3168 PSI Gapped 0.0432 0.2670