Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -1 -2 -3 -4 -2 -1 -3 -3 -2 1 2 -2 8 0 -3 -2 -1 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.76 0.17 2 D -2 -2 1 7 -4 -1 2 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 2 89 0 0 9 0 0 0 0 0 0 0 0 0 0 0 0 0 1.11 0.17 3 P 0 -2 -1 -1 -2 -1 -1 -1 -2 -3 -2 -1 -2 -3 4 4 0 -3 0 -2 0 0 0 0 0 0 0 0 0 0 3 0 0 0 25 69 0 0 3 0 0.66 0.18 4 N -2 -1 7 1 -3 -1 -1 -1 0 -3 -4 -1 -3 0 -3 0 0 -4 -2 -3 0 0 89 0 0 0 0 0 0 0 0 0 0 5 0 0 6 0 0 0 0.88 0.18 5 T -1 -2 -1 -2 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.78 0.18 6 V -1 -3 -3 -4 -2 -3 -3 -4 -4 3 0 -3 2 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 15 0 0 6 0 0 0 6 0 0 73 0.52 0.18 7 S 1 -2 0 -1 -1 -1 -1 -1 -2 -1 -1 -1 -1 -2 -2 4 2 -3 -2 -1 6 0 0 0 0 0 0 0 0 6 8 0 0 0 0 66 14 0 0 0 0.42 0.18 8 S 1 -1 0 -1 -1 -1 -1 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 6 0 0 0 0 0 0 0 0 0 1 0 0 0 0 87 2 0 0 0 0.59 0.17 9 F -3 -3 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 0 7 -4 -3 -3 0 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.11 0.17 10 Q -1 1 0 -1 -4 7 1 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 97 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0.94 0.17 11 V -1 -3 -4 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.63 0.17 12 D -1 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 5 0 0 95 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.13 0.17 13 C -1 -4 -3 -4 10 -4 -4 -3 -4 -2 -2 -4 -2 -3 -4 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.72 0.18 14 F -3 -3 -4 -4 -3 -3 -4 -4 -1 -1 0 -3 -1 7 -4 -3 -3 1 5 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 79 0 0 0 0 21 0 1.03 0.18 15 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 1 -4 -3 -2 -2 -1 0 0 0 0 0 0 0 0 0 0 3 95 0 0 2 0 0 0 0 0 0 0.69 0.18 16 W -3 -3 -5 -5 -3 -3 -4 -3 -3 -3 -2 -4 -2 0 -4 -4 -3 12 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.78 0.19 17 H -2 -1 0 -2 -4 0 -1 -3 9 -4 -4 -1 -2 -2 -3 -1 -2 -3 1 -4 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 1.36 0.19 18 V -1 -3 -4 -4 -2 -3 -4 -4 -4 4 1 -3 1 -1 -3 -3 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 39 10 0 0 0 0 0 0 0 0 51 0.56 0.19 19 R -2 6 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -4 -3 0 -2 -3 -2 -3 0 86 0 0 0 0 0 0 0 0 0 10 0 0 0 4 0 0 0 0 0.94 0.19 20 K -1 2 -1 -1 -4 2 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 2 0 0 0 4 0 0 0 0 0 93 0 0 0 0 0 0 0 0 0.79 0.19 21 R -2 5 -1 -2 -3 2 -1 -3 -1 -2 0 1 1 -3 -3 -1 0 -3 -2 -2 0 64 0 0 0 13 0 0 0 0 12 2 5 0 0 0 5 0 0 0 0.60 0.19 22 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 2 -3 0 6 -2 -3 -2 -1 1 1 0 0 0 0 0 0 0 0 0 2 23 0 0 58 2 0 0 0 0 16 0.62 0.19 23 A 4 -2 -2 -2 -1 -1 -1 -1 -2 -1 -2 -1 -1 -3 -2 2 0 -3 -2 0 69 0 0 0 0 0 0 0 0 0 0 0 0 0 0 22 0 0 0 9 0.47 0.18 24 D -2 -2 1 6 -4 -1 1 -2 -2 -2 -1 -1 2 -3 -2 -1 -1 -4 -3 -3 0 0 4 76 0 0 0 0 0 0 4 0 13 0 0 0 2 0 0 0 0.81 0.19 25 Q -2 2 2 -1 -1 5 0 -2 -1 -3 -2 0 1 -2 -2 0 -1 6 -1 -3 0 13 12 0 2 47 0 0 0 0 0 2 8 0 0 3 0 13 0 0 0.54 0.19 26 E -1 0 0 3 -4 0 4 3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 3 0 16 0 0 46 33 0 0 0 2 0 0 0 0 0 0 0 0 0.60 0.16 27 L -2 -2 -3 -3 -2 -2 -1 -4 -3 1 4 -2 4 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 7 0 0 0 69 0 24 0 0 0 0 0 0 0 0.50 0.16 28 G 0 -3 -1 -2 3 -2 -3 5 -3 -3 -3 -2 -3 -3 -3 0 -1 -3 -3 -1 0 0 0 0 10 0 0 73 0 0 0 0 0 0 0 9 0 0 0 7 0.81 0.16 29 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.18 0.14 30 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.61 0.14 31 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -2 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.21 0.14 32 F -3 -3 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 0 7 -4 -3 -3 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.05 0.14 33 L -2 -2 -3 0 -2 -2 -1 -4 -3 1 4 -2 1 0 -2 -2 -1 -3 -2 0 0 0 0 9 0 0 6 0 0 5 75 0 0 0 2 0 3 0 0 0 0.38 0.14 34 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -3 -4 -2 -1 -2 -5 -3 -4 0 0 0 94 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.04 0.14 35 R -2 6 -1 -2 -4 1 -1 -3 -1 -3 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 94 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.89 0.14 36 L -2 -3 -4 -4 -2 -3 -3 -4 -3 2 5 -3 2 0 -3 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 4 96 0 0 0 0 0 0 0 0 0 0.65 0.14 37 R -2 6 -1 -2 -4 1 -1 -2 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 97 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0.95 0.14 38 R -2 6 -1 -2 -4 0 -1 -1 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 94 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0.94 0.16 39 D -2 -2 1 7 -4 -1 2 -2 -1 -4 -4 -1 -4 -4 -2 -1 -2 -4 -1 -4 0 0 0 90 0 0 7 0 0 0 0 0 0 0 0 0 0 0 3 0 1.07 0.16 40 Q -1 1 0 -1 -4 7 2 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.94 0.16 41 K -1 3 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -2 -1 -1 -4 -2 -3 0 9 0 0 0 0 0 0 0 0 0 91 0 0 0 0 0 0 0 0 0.74 0.16 42 S 2 -1 0 -1 -1 -1 -1 -1 -2 -3 -3 -1 -2 -1 -1 4 1 -3 -2 -2 23 0 0 0 0 0 0 0 0 0 0 0 0 3 0 74 0 0 0 0 0.51 0.16 43 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.69 0.16 44 R -2 5 -1 -2 -4 1 0 -2 -1 -3 -3 5 -2 -4 -2 -1 -1 -3 -2 -3 0 46 0 0 0 0 0 0 0 0 0 54 0 0 0 0 0 0 0 0 0.75 0.16 45 G 0 -1 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 4 0 0 0 0 0 96 0 0 0 0 0 0 0 0 0 0 0 0 1.19 0.16 46 R -2 7 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.03 0.16 47 G 0 -2 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -2 2 -1 -3 -3 -3 0 0 0 0 0 0 0 75 0 0 0 0 0 0 0 25 0 0 0 0 0.91 0.16 48 N 0 -1 3 -1 -2 -1 -1 -1 -1 -1 -2 -1 1 -2 -2 4 0 -3 0 -2 0 0 25 0 0 0 0 0 0 7 0 0 7 0 0 57 0 0 4 0 0.37 0.16 49 T -1 -2 -1 -2 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.73 0.16 50 L -2 -3 -4 -4 -2 -3 -4 -4 -3 2 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 4 96 0 0 0 0 0 0 0 0 0 0.67 0.16 51 G 0 -3 -1 -2 -3 -2 -2 6 -3 -4 -4 -2 -3 -4 -3 0 -2 -3 -4 -4 0 0 0 0 0 0 0 90 0 0 0 0 0 0 0 6 0 0 0 0 1.10 0.16 52 L -2 -3 -4 -4 -2 -3 -4 -4 -3 2 5 -3 2 0 -3 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 10 84 0 3 0 0 0 0 0 0 3 0.60 0.16 53 D -2 -2 3 6 -4 0 2 -1 -1 -4 -4 -1 -3 -4 -2 -1 -1 -5 -3 -4 2 0 17 68 0 0 11 2 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.16 54 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 2 -3 1 -1 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 93 7 0 0 0 0 0 0 0 0 0 0.64 0.16 55 E -1 2 -1 0 -4 2 5 -2 -1 -4 -3 2 -2 -4 -2 -1 -2 -4 -3 -3 2 13 0 0 0 9 64 2 0 0 0 9 0 0 0 0 0 0 0 0 0.71 0.25 56 T 2 -2 -1 -2 -2 -1 -2 -2 -2 -1 -2 0 -2 -3 2 2 4 -4 -3 1 22 0 0 0 0 0 0 0 0 2 0 7 0 0 9 13 37 0 0 11 0.36 0.25 57 A 5 -2 -2 -3 -1 -2 -2 -1 -2 -2 -2 -2 -2 -3 -2 1 -1 -3 -3 0 91 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 0 0 0 4 0.68 0.25 58 T -1 -2 -1 -2 -2 -1 -2 -2 -3 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.88 0.25 59 R -2 4 -2 -3 2 1 -2 -3 4 0 1 0 2 0 -3 -2 -2 -3 0 -1 0 33 0 0 7 5 0 0 13 6 19 4 7 3 0 0 0 0 3 0 0.32 0.25 60 A 4 -2 -2 -2 -2 -1 2 -2 -2 0 -2 -1 -1 -3 -2 0 0 -4 -2 2 49 0 0 0 0 0 21 0 0 2 0 0 0 0 0 3 2 0 0 22 0.35 0.25 61 G -1 -3 -1 -2 -3 -3 -3 7 -3 -5 -5 -2 -4 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.41 0.25 62 K -2 2 -1 -2 -4 0 0 -2 -1 -3 -3 6 -2 -2 -2 -1 -2 -3 2 -3 0 5 0 0 0 0 0 0 0 0 0 86 0 0 0 0 0 0 9 0 0.77 0.25 63 Q -2 2 -1 -1 -4 6 1 -3 3 -3 -2 2 -1 -2 -2 -1 -2 -2 2 -3 0 7 0 0 0 62 0 0 7 0 2 13 0 0 0 0 0 0 9 0 0.67 0.25 64 I -2 -3 -4 -4 -2 -3 -3 -4 -4 6 1 0 0 -1 -3 -3 -1 -4 -2 2 0 0 0 0 0 0 0 0 0 86 0 9 0 0 0 0 2 0 0 3 0.63 0.25 65 V -1 -3 -4 -4 -2 -3 -3 -4 -4 3 1 -3 1 -1 -3 -3 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 16 3 0 2 0 0 0 0 0 0 78 0.66 0.25 66 E -2 0 -1 0 -4 1 5 -2 -1 -4 -4 3 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 72 3 0 0 0 26 0 0 0 0 0 0 0 0 0.82 0.25 67 R -2 5 -1 1 -4 1 0 -3 0 -4 -3 2 -2 -3 -3 0 -2 7 -1 -3 0 42 0 8 0 5 3 0 2 0 0 12 0 0 0 10 0 18 0 0 0.66 0.25 68 I -2 -4 -4 -4 -2 -4 -4 -5 -4 6 1 -4 1 -1 -4 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0.83 0.25 69 L -2 -3 -4 -4 -2 -3 -3 -4 -4 1 5 -3 1 0 -4 -3 1 -3 -2 0 0 0 0 0 0 0 0 0 0 0 90 0 0 0 0 0 10 0 0 0 0.69 0.25 70 E -2 1 -1 1 -4 1 4 -2 -1 -4 -4 4 -3 -4 -2 -1 -2 -4 -3 -3 0 4 0 4 0 2 44 2 0 0 0 44 0 0 0 0 0 0 0 0 0.70 0.27 71 E -1 -1 0 2 -4 1 5 1 -1 -4 -4 0 -3 -4 -2 0 -2 -4 -3 -3 0 0 4 5 0 0 74 12 0 0 0 2 0 0 0 4 0 0 0 0 0.78 0.27 72 E -2 -1 -1 1 -5 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 3 0 0 97 0 0 0 0 0 0 0 0 0 0 0 0 0 1.06 0.23 73 G 1 -1 0 -1 -1 -1 -1 0 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 97 0 0 0 0 0.66 0.17 74 G 0 -1 4 0 -2 0 -1 2 0 -3 -3 -1 -2 -3 -2 3 0 -3 -2 -3 0 0 41 0 0 0 0 14 0 0 0 0 0 0 0 44 0 0 0 0 0.46 0.06 K Lambda Standard Ungapped 0.1389 0.3199 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1651 0.3171 PSI Gapped 0.0505 0.2670