Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -1 -2 -3 -4 -2 -1 -3 -3 -2 1 2 -2 7 0 -3 -2 -1 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.67 0.13 2 E -2 -2 -3 -3 -2 -2 1 -4 -2 0 4 -2 1 3 -3 -3 -2 -1 0 0 0 0 0 0 0 0 14 0 0 0 63 0 0 23 0 0 0 0 0 0 0.39 0.13 3 T -1 -2 -1 -2 -1 -1 -2 -2 -2 1 -1 -1 -1 -2 -2 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 12 0 0 0 0 0 0 88 0 0 0 0.57 0.13 4 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 -3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 5 K -1 2 -1 -1 -4 1 0 -2 -1 -3 -3 6 -2 -4 -1 -1 -1 -4 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.74 0.13 6 I -1 -3 -3 -3 -2 -3 -3 -4 -3 4 3 -3 1 0 -3 0 -1 -3 -2 1 0 0 0 0 0 0 0 0 0 46 42 0 0 0 0 12 0 0 0 0 0.38 0.13 7 T -2 -3 -3 -4 -2 -3 -3 -4 -2 0 2 -3 0 6 -3 -2 1 0 1 1 0 0 0 0 0 0 0 0 0 0 18 0 0 55 0 0 14 0 0 12 0.48 0.13 8 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.56 0.13 9 Y -2 -2 -2 -3 -3 -1 -2 -3 5 -2 -2 -2 -2 2 -3 -2 -2 1 7 -2 0 0 0 0 0 0 0 0 18 0 0 0 0 0 0 0 0 0 82 0 1.00 0.13 10 I 0 -2 -1 -2 -1 -1 -1 -2 -2 1 -1 -1 -1 -2 -2 2 5 -3 -2 0 0 0 0 0 0 0 0 0 0 14 0 0 0 0 0 11 75 0 0 0 0.49 0.13 11 V -1 -3 -3 -3 5 -3 -3 -3 -4 2 0 -3 0 -2 -3 -1 1 -3 -2 4 0 0 0 0 20 0 0 0 0 13 0 0 0 0 0 0 12 0 0 55 0.44 0.13 12 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.56 0.13 13 T 2 1 -2 -3 -2 -2 -2 -2 -3 3 0 -1 0 -2 -2 0 2 -3 -2 1 23 10 0 0 0 0 0 0 0 41 0 0 0 0 0 0 27 0 0 0 0.22 0.13 14 F -1 -2 -2 -3 -2 -2 -2 -2 -1 -1 -1 -2 -1 6 -3 2 -1 0 3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 59 0 30 0 0 10 0 0.55 0.13 15 F -2 -3 -3 -4 -3 -3 -3 -4 -2 -1 0 -3 -1 7 3 -3 -2 0 2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 82 18 0 0 0 0 0 0.87 0.13 16 V -1 -3 -3 -3 3 -3 -3 -3 -3 3 0 -3 0 -1 -3 0 0 -3 -2 4 0 0 0 0 11 0 0 0 0 23 0 0 0 0 0 9 0 0 0 57 0.39 0.13 17 L 0 -2 0 -1 -2 -1 -1 -1 -1 -2 0 -1 -1 -1 -2 4 1 -2 2 -2 0 0 0 0 0 0 0 0 0 0 14 0 0 0 0 73 0 0 13 0 0.36 0.13 18 L -2 -2 -3 -4 -2 -2 -3 -4 3 1 4 -3 1 0 -3 -3 -2 -2 -1 0 0 0 0 0 0 0 0 0 13 0 87 0 0 0 0 0 0 0 0 0 0.52 0.13 19 F -2 -3 -3 -4 -3 -3 -3 -4 -2 -1 0 -3 -1 7 3 -3 -2 0 2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 82 18 0 0 0 0 0 0.87 0.13 20 V -1 -3 -3 -3 -2 -3 -3 -4 -4 4 1 -3 0 -1 -3 -2 1 -3 -2 3 0 0 0 0 0 0 0 0 0 55 0 0 0 0 0 0 12 0 0 33 0.44 0.13 21 F -2 -3 -3 -4 -3 -3 -3 -4 -2 -1 0 -3 -1 7 3 -3 -2 0 2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 82 18 0 0 0 0 0 0.87 0.13 22 G 0 1 -1 -2 -3 -2 -2 6 -2 -4 -4 -1 -3 -4 -3 -1 -2 -3 -3 -4 0 13 0 0 0 0 0 87 0 0 0 0 0 0 0 0 0 0 0 0 1.02 0.13 23 F -2 -3 -2 -3 -3 -3 -3 -3 -2 -1 0 -3 -1 7 -3 1 -2 0 2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 82 0 18 0 0 0 0 0.73 0.13 24 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 4 -3 1 0 3 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 82 0 0 0 18 0 0 0 0 0 0.58 0.13 25 S 1 -1 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 3 5 1 -4 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 82 0 0 0 0 0.64 0.13 26 G -2 -1 6 1 -3 -1 -1 2 0 -4 -4 -1 -3 -4 -3 0 -1 -4 -3 -4 0 0 86 0 0 0 0 14 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.13 27 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.16 0.13 28 P 1 -3 -2 -2 -3 -2 -2 -2 -3 -3 -3 -1 -3 -4 8 -1 -1 -4 -3 -3 12 0 0 0 0 0 0 0 0 0 0 0 0 0 88 0 0 0 0 0 1.85 0.13 29 A 2 -2 -1 -1 -2 -1 -2 5 -2 -3 -3 -1 -3 -3 -2 2 0 -3 -3 -3 14 0 0 0 0 0 0 54 0 0 0 0 0 0 0 32 0 0 0 0 0.64 0.13 30 R -2 7 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.99 0.13 31 N -2 2 6 1 -3 0 -1 -1 0 -4 -4 0 -3 -4 -3 0 -1 -4 -3 -3 0 12 88 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.13 32 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.20 0.13 33 K -1 -1 4 -1 -3 -1 -1 5 -1 -4 -4 1 -3 -4 -2 0 -1 -4 -3 -4 0 0 32 0 0 0 0 54 0 0 0 14 0 0 0 0 0 0 0 0 0.68 0.13 34 R -2 7 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.99 0.13 35 K -1 1 -1 0 -4 1 3 -2 -1 -3 -3 5 -2 -4 -2 -1 -1 -4 -2 -3 0 0 0 0 0 0 23 0 0 0 0 77 0 0 0 0 0 0 0 0 0.64 0.13 36 D -2 2 0 5 -4 0 3 -2 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 16 0 56 0 0 29 0 0 0 0 0 0 0 0 0 0 0 0 0 0.72 0.11 37 L -2 -3 -4 -4 -2 -3 -3 -4 -3 3 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 24 76 0 0 0 0 0 0 0 0 0 0.50 0.08 38 E -1 0 0 1 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.82 0.06 K Lambda Standard Ungapped 0.1497 0.3319 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1615 0.3168 PSI Gapped 0.0494 0.2670