Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -1 -1 -2 -3 -1 0 -2 -3 -2 1 2 -1 5 0 -2 -1 -1 -1 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 2 E -1 2 0 3 -4 5 2 -2 0 -3 -3 1 -1 -4 -2 -1 -1 -3 -2 -3 0 11 0 19 0 58 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0.61 0.10 3 P -1 1 -2 -2 -3 -1 -1 -2 -2 -3 -3 -1 -3 -4 7 0 -1 -4 -3 -3 0 14 0 0 0 0 0 0 0 0 0 0 0 0 77 9 0 0 0 0 1.52 0.10 4 N -2 2 6 1 -3 0 -1 -1 0 -4 -4 0 -3 -4 -3 0 -1 -4 -3 -3 0 15 85 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.81 0.13 5 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -4 -1 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.20 0.13 6 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.97 0.13 7 R -1 2 0 2 -3 1 2 -2 -1 -3 -3 4 -2 -4 -2 1 -1 -4 -3 -3 0 9 0 15 0 0 18 0 0 0 0 40 0 0 0 18 0 0 0 0 0.44 0.13 8 Q 1 0 -1 -1 -3 5 1 -2 -1 -2 1 0 0 -3 -2 -1 -1 -3 -2 -2 15 0 0 0 0 67 0 0 0 0 18 0 0 0 0 0 0 0 0 0 0.46 0.13 9 P 0 -2 -1 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 6 4 0 -4 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 42 58 0 0 0 0 0.90 0.13 10 V -1 -3 -4 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.56 0.13 11 E -1 -1 0 3 -4 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 15 0 0 85 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.13 12 L -1 -2 -2 -3 -2 -2 -2 -3 -3 1 4 -2 1 0 -3 1 -1 -2 -2 0 0 0 0 0 0 0 0 0 0 0 75 0 0 0 0 25 0 0 0 0 0.38 0.13 13 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.97 0.13 14 R -2 7 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.13 15 T 0 -2 0 -2 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.69 0.13 16 S 1 -1 0 -1 -1 0 -1 -1 -1 -3 -3 -1 -2 -3 -1 5 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.63 0.13 17 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.65 0.13 18 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -2 -1 3 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.15 0.13 19 L -3 0 -4 -4 -3 -2 -3 -3 -3 -1 1 -2 0 0 -4 -3 -2 10 1 -2 0 8 0 0 0 0 0 0 0 0 27 0 0 0 0 0 0 65 0 0 1.50 0.13 20 G 0 -3 -1 -2 -3 -2 -3 6 -3 -4 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.24 0.13 21 L -2 -3 -4 -4 -2 -3 -3 -4 -3 2 4 -3 1 0 2 -3 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 18 72 0 0 0 10 0 0 0 0 0 0.50 0.13 22 L -2 -3 -4 -4 -2 -3 -3 -4 -3 1 5 -3 2 0 1 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 91 0 0 0 9 0 0 0 0 0 0.57 0.13 23 L -2 -3 -3 -3 -2 -2 -3 -4 -3 0 4 -3 1 3 1 -2 1 -2 0 0 0 0 0 0 0 0 0 0 0 0 59 0 0 18 8 0 15 0 0 0 0.36 0.13 24 I -2 -4 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 82 0 0 0 0 0 0 0 0 0 18 0.57 0.13 25 L 1 -3 -3 -4 4 -3 -3 -3 -2 -1 0 -3 -1 5 2 -2 -2 -1 1 -1 18 0 0 0 15 0 0 0 0 0 9 0 0 47 10 0 0 0 0 0 0.44 0.13 26 V 1 -3 -3 -3 -1 -2 -2 -3 -3 2 0 -2 0 -2 -2 -1 2 -3 -2 4 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 0 0 72 0.37 0.13 27 L -2 -3 -3 -3 5 -3 -3 -4 -3 0 3 -3 0 -1 4 -2 -2 -3 -2 0 0 0 0 0 18 0 0 0 0 0 55 0 0 0 27 0 0 0 0 0 0.60 0.13 28 A 4 -2 -1 -2 -1 -1 -1 2 -2 -2 -2 -1 -2 -3 -1 2 0 -3 -3 -1 67 0 0 0 0 0 0 18 0 0 0 0 0 0 0 15 0 0 0 0 0.47 0.13 29 L -1 -3 -4 -4 -1 -3 -3 -4 -4 3 1 -3 1 -1 -3 -2 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 16 10 0 0 0 0 0 0 0 0 73 0.49 0.13 30 L -1 -2 -2 -2 -2 -2 -2 -2 -3 0 3 -2 0 -1 3 2 0 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 50 0 0 0 17 32 0 0 0 0 0.35 0.13 31 F -2 -3 -2 -3 -3 -3 -3 -3 -2 -1 0 -3 -1 7 -3 1 -2 0 2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 81 0 19 0 0 0 0 0.72 0.13 32 S 0 -2 0 -1 -2 -1 -1 -1 -2 -3 -3 -1 -2 -3 4 4 0 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 22 68 0 0 0 0 0.54 0.13 33 S -1 -1 5 0 -2 0 -1 -1 0 -3 -4 -1 -2 -3 -2 3 0 -4 -2 -3 0 0 59 0 0 0 0 0 0 0 0 0 0 0 0 41 0 0 0 0 0.64 0.13 34 Y -2 -2 -1 -3 -3 -1 -2 -3 6 -2 -2 -2 -2 2 -3 -2 -2 1 7 -2 0 0 0 0 0 0 0 0 32 0 0 0 0 0 0 0 0 0 68 0 0.93 0.13 35 F -2 -3 -3 -3 -3 -3 -3 -3 -2 -1 0 -3 -1 7 -4 0 -2 0 2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 86 0 14 0 0 0 0 0.78 0.13 36 F -2 -3 -3 -3 -3 -3 -3 -3 -2 -1 0 -3 -1 7 -4 0 -2 0 2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 86 0 14 0 0 0 0 0.78 0.13 37 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 -1 -3 -4 -3 0 0 -4 -3 -4 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.97 0.13 K Lambda Standard Ungapped 0.1453 0.3274 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1580 0.3181 PSI Gapped 0.0484 0.2670