Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 M -1 -2 -2 -3 -2 -1 -2 -3 -2 1 2 -2 7 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.46 0.05 2 Q -1 1 0 -1 -3 5 1 -2 0 -3 -3 3 -1 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 61 0 0 0 0 0 39 0 0 0 0 0 0 0 0 0.58 0.05 3 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -2 -2 3 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.24 0.17 4 K 1 3 -1 -2 -3 1 0 -2 -1 -3 -3 5 -2 -4 -2 0 -1 -4 -2 -2 18 16 0 0 0 0 0 0 0 0 0 67 0 0 0 0 0 0 0 0 0.57 0.17 5 L -2 -3 -4 -4 -2 -3 -4 -4 -3 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.71 0.17 6 I -1 -3 -4 -4 -2 -3 -4 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 57 0 0 0 0 0 0 0 0 0 43 0.58 0.17 7 L -2 -3 -4 -4 -2 -3 -4 -4 -4 4 4 -3 1 0 -4 -3 -2 -3 -2 1 0 0 0 0 0 0 0 0 0 43 57 0 0 0 0 0 0 0 0 0 0.57 0.17 8 N -2 0 6 0 -4 0 -1 -1 0 -4 -4 3 -3 -4 -2 0 -1 -4 -3 -4 0 0 75 0 0 0 0 0 0 0 0 25 0 0 0 0 0 0 0 0 0.87 0.23 9 G -1 -3 -1 -2 -3 -2 -3 5 -3 -2 1 -2 -1 -3 -3 -1 2 -3 -3 -2 0 0 0 0 0 0 0 60 0 0 22 0 0 0 0 0 18 0 0 0 0.63 0.23 10 K -1 3 -1 -2 -4 0 0 -3 -2 -2 -3 5 -2 -4 -2 -1 -1 -4 -3 0 0 12 0 0 0 0 0 0 0 0 0 76 0 0 0 0 0 0 0 12 0.67 0.24 11 T -1 -1 -1 -2 -2 3 -1 2 -2 -2 -3 -1 -2 -3 -2 0 5 -3 -3 -2 0 0 0 0 0 21 0 22 0 0 0 0 0 0 0 0 56 0 0 0 0.54 0.24 12 L 1 -1 -3 -3 -2 -2 -2 -3 -3 0 3 2 0 2 -3 -1 -2 -2 -1 -1 21 0 0 0 0 0 0 0 0 0 45 22 0 12 0 0 0 0 0 0 0.28 0.24 13 K -1 1 3 -1 -3 0 0 -2 -1 -3 -3 5 -2 -4 -2 1 1 -4 -3 -3 0 0 22 0 0 0 0 0 0 0 0 55 0 0 0 12 11 0 0 0 0.54 0.24 14 G -1 -3 -1 -1 -4 -1 2 6 -2 -5 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 21 79 0 0 0 0 0 0 0 0 0 0 0 0 1.04 0.25 15 V 2 -1 -1 0 -3 0 5 -2 -2 -2 -3 -1 -2 -3 -2 0 1 -4 -3 0 26 0 0 0 0 0 55 0 0 0 0 0 0 0 0 0 11 0 0 8 0.50 0.25 16 L 2 -2 -1 -2 -2 -2 -2 -2 -3 -1 0 -2 -1 -3 -2 1 5 -3 -2 -1 21 0 0 0 0 0 0 0 0 0 8 0 0 0 0 0 71 0 0 0 0.58 0.25 17 T 1 -2 -1 -2 -2 -2 -2 -2 -3 2 -1 -2 -1 -2 -2 0 5 -3 -2 0 10 0 0 0 0 0 0 0 0 21 0 0 0 0 0 0 70 0 0 0 0.56 0.25 18 I -1 -1 -2 -2 -2 -1 -1 -3 -3 1 -1 3 -1 -3 -2 -1 3 -4 -2 2 0 0 0 0 0 0 0 0 0 8 0 32 0 0 0 0 34 0 0 26 0.35 0.25 19 E -2 0 -1 0 -4 1 5 -3 -1 -4 -4 4 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 0 0 0 59 0 0 0 0 41 0 0 0 0 0 0 0 0 0.80 0.25 20 A 5 -2 -3 -3 -1 -2 -2 -1 -3 -1 1 -2 -1 -2 -2 0 -1 -3 -2 -1 79 0 0 0 0 0 0 0 0 0 21 0 0 0 0 0 0 0 0 0 0.54 0.25 21 V -1 -2 -3 -3 -2 -2 -3 0 -3 2 0 0 0 -2 -3 0 -1 -4 -2 4 0 0 0 0 0 0 0 10 0 11 0 8 0 0 0 9 0 0 0 63 0.38 0.25 22 D -1 -2 2 6 -3 -1 0 -2 -2 -4 -4 -1 -3 -4 -2 2 -1 -5 -3 -4 0 0 10 63 0 0 0 0 0 0 0 0 0 0 0 27 0 0 0 0 0.85 0.25 23 A 5 -2 -2 -3 -2 -2 -2 -1 -3 -2 -3 -2 -2 -3 4 0 -1 -4 -3 -1 80 0 0 0 0 0 0 0 0 0 0 0 0 0 20 0 0 0 0 0 0.75 0.25 24 A 3 -2 -2 -1 -2 0 4 1 -2 -3 -3 -1 -2 -4 -2 0 -1 -4 -3 -2 45 0 0 0 0 0 42 12 0 0 0 0 0 0 0 0 0 0 0 0 0.53 0.25 25 T 1 -2 -1 -1 -2 1 2 -2 -2 1 -2 -1 -1 -3 -2 0 4 -3 -2 -1 11 0 0 0 0 9 17 0 0 12 0 0 0 0 0 0 51 0 0 0 0.40 0.25 26 A 5 -2 -2 -3 -1 -2 -2 -1 -2 -2 -2 -1 -2 -3 -2 0 -1 -3 -3 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.77 0.25 27 E -2 2 -1 1 -5 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 14 0 0 0 0 86 0 0 0 0 0 0 0 0 0 0 0 0 0 0.96 0.25 28 K -1 1 -1 -1 -3 2 0 -2 -1 -4 -3 5 -2 -4 -2 1 -1 -4 -3 -3 0 0 0 0 0 10 0 0 0 0 0 77 0 0 0 14 0 0 0 0 0.71 0.25 29 V -1 -3 -3 -4 -3 -2 -3 -3 0 -1 -1 -3 -1 3 -3 -2 0 1 7 1 5 0 0 0 0 0 0 0 0 0 0 0 0 14 0 0 10 0 58 14 0.74 0.25 30 F -3 -4 -4 -4 -3 -4 -4 -4 -2 1 0 -4 -1 7 -4 -3 -3 0 2 -1 0 0 0 0 0 0 0 0 0 12 0 0 0 88 0 0 0 0 0 0 1.08 0.25 31 K -2 5 -1 -2 -4 0 0 -3 -1 -4 -3 4 -2 -4 3 -1 -2 -4 -3 -3 0 38 0 0 0 0 0 0 0 0 0 49 0 0 14 0 0 0 0 0 0.78 0.25 32 Q -2 -1 3 2 -3 4 0 -3 -1 1 1 -1 -1 -2 -3 -1 -1 -3 -2 -1 0 0 26 11 0 29 0 0 0 14 21 0 0 0 0 0 0 0 0 0 0.34 0.25 33 Y -3 -3 -4 -4 -3 -3 -3 -4 0 1 1 -3 -1 3 -4 -3 -2 7 6 -1 0 0 0 0 0 0 0 0 0 12 14 0 0 9 0 0 0 17 48 0 0.89 0.25 34 A 5 -2 -2 -2 -1 -1 1 -1 -2 -2 -2 -1 -2 -3 -2 0 -1 -3 -3 0 77 0 0 0 0 0 14 0 0 0 0 0 0 0 0 0 0 0 0 9 0.54 0.25 35 N -2 -1 7 0 -3 -1 -1 -1 0 -4 -4 -1 -3 -4 -3 1 1 -4 -3 -3 0 0 80 0 0 0 0 0 0 0 0 0 0 0 0 10 11 0 0 0 0.92 0.25 36 D 2 -2 0 3 -3 0 3 -2 -2 -3 -3 -1 -3 -4 -2 2 -1 -4 -3 -3 21 0 0 23 0 0 32 0 0 0 0 0 0 0 0 25 0 0 0 0 0.51 0.25 37 L -2 0 6 0 -3 0 -1 -2 -1 -3 -1 2 -2 -3 -2 1 -1 -4 -3 -3 0 0 57 0 0 0 0 0 0 0 8 21 0 0 0 14 0 0 0 0 0.60 0.25 38 G -1 -3 -1 1 -3 -2 -2 5 -3 -3 -4 -2 -3 -4 -2 1 2 -4 -3 -3 0 0 0 8 0 0 0 58 0 0 0 0 0 0 0 14 21 0 0 0 0.72 0.25 39 V -1 -3 -4 -4 -2 -3 -3 -4 -4 3 2 -3 1 -1 -3 -3 -1 -3 -2 5 0 0 0 0 0 0 0 0 0 10 26 0 0 0 0 0 0 0 0 64 0.59 0.25 40 D -2 -2 0 5 -4 -1 3 -2 -2 -4 -4 -1 -3 -4 3 -1 1 -5 -4 -3 0 0 0 56 0 0 21 0 0 0 0 0 0 0 14 0 10 0 0 0 0.83 0.25 41 G 1 -3 -2 -3 -3 -3 -3 5 -3 -2 1 -2 -1 -2 -3 -1 -2 -3 -3 -2 11 0 0 0 0 0 0 63 0 0 26 0 0 0 0 0 0 0 0 0 0.70 0.25 42 E -1 -1 -1 2 -3 0 5 -3 -1 -1 -2 -1 -2 -3 -2 -1 -1 -4 -3 2 0 0 0 12 0 0 57 0 0 0 0 0 0 0 0 0 0 0 0 31 0.50 0.19 43 W -3 -3 -5 -5 -3 -3 -4 -3 -3 -3 -2 -4 -2 0 -4 -3 -3 12 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.78 0.19 44 T 2 -2 -1 -2 -1 -1 -1 -1 -2 -2 -2 -1 -2 -3 -2 3 4 -3 -2 -1 22 0 0 0 0 0 0 0 0 0 0 0 0 0 0 31 46 0 0 0 0.49 0.19 45 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -1 1 -3 -1 2 -4 -2 -2 1 8 -1 0 0 0 0 0 0 0 0 0 0 14 0 0 0 0 0 0 0 86 0 1.04 0.19 46 D -2 -1 1 6 -4 0 1 -2 -2 -4 -4 2 -3 -4 -2 -1 -2 -5 -3 -4 0 0 0 76 0 0 0 0 0 0 0 24 0 0 0 0 0 0 0 0 0.92 0.19 47 D -2 -1 0 5 -4 0 3 -2 -1 -3 -4 1 -3 -4 -2 -1 1 -4 -3 -3 0 0 0 46 0 0 31 0 0 0 0 12 0 0 0 0 11 0 0 0 0.68 0.19 48 A 3 -2 -1 3 -2 0 3 -1 -2 -3 -3 -1 -2 -3 -2 1 -1 -4 -3 -2 45 0 0 20 0 0 24 0 0 0 0 0 0 0 0 12 0 0 0 0 0.43 0.19 49 T 0 -2 -1 -2 -2 -1 -2 -2 -2 1 -1 -1 -1 -2 -2 2 5 -3 -2 0 0 0 0 0 0 0 0 0 0 14 0 0 0 0 0 21 65 0 0 0 0.50 0.19 50 K -2 4 -1 -2 -4 0 0 -3 -1 -2 0 5 -1 -3 -2 -1 -1 -3 -2 -2 0 23 0 0 0 0 0 0 0 0 14 62 0 0 0 0 0 0 0 0 0.56 0.19 51 T -1 -2 -1 -2 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.79 0.19 52 F -3 -4 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 -1 7 -4 -3 -3 0 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.15 0.19 53 T -1 -2 -1 -2 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -2 1 6 -3 -2 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.79 0.19 54 V 2 -3 -3 -3 -1 -2 -3 -2 -3 1 0 -2 0 -2 -2 -1 -1 -3 -2 4 31 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 69 0.44 0.19 55 T 1 3 -1 -2 -2 -1 -1 -2 -2 0 -2 0 -1 -3 -2 0 4 -3 -2 -1 12 21 0 0 0 0 0 0 0 10 0 0 0 0 0 0 57 0 0 0 0.41 0.19 56 E -1 -1 -1 1 -4 1 6 -3 -1 -4 -4 0 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 1.02 0.19 K Lambda Standard Ungapped 0.1302 0.3132 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1550 0.3175 PSI Gapped 0.0474 0.2670