Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 V -1 -3 -4 -4 -1 -3 -3 -4 -4 4 1 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 55 0 0 0 0 0 0 0 0 0 45 0.45 0.07 2 L -2 -3 -4 -4 -2 -3 -4 -4 -3 3 4 -3 2 0 -3 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 22 78 0 0 0 0 0 0 0 0 0 0.55 0.12 3 T -1 -1 -1 1 -3 1 5 -2 -1 -3 -3 0 -2 -3 -2 0 3 -3 -2 -2 0 0 0 0 0 0 66 0 0 0 0 0 0 0 0 0 34 0 0 0 0.59 0.12 4 E -1 0 -1 1 -4 2 6 -3 0 -4 -3 0 -3 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.91 0.12 5 G -1 1 -1 -2 -3 -2 -2 6 -2 -4 -4 -1 -3 -4 -3 -1 -2 -3 -4 -4 0 14 0 0 0 0 0 86 0 0 0 0 0 0 0 0 0 0 0 0 1.06 0.18 6 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -3 -4 -2 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.25 0.18 7 P 2 -2 -2 -2 -2 2 -1 -2 -2 -2 0 -1 -1 -3 6 -1 -1 -3 -3 -2 23 0 0 0 0 18 0 0 0 0 16 0 0 0 43 0 0 0 0 0 0.73 0.18 8 V -1 -3 -4 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.63 0.18 9 K -2 3 -2 -2 -3 0 -1 -3 -2 -1 2 4 3 -2 -2 -1 -1 -3 -2 -1 0 16 0 0 0 0 0 0 0 0 23 43 18 0 0 0 0 0 0 0 0.36 0.18 10 I -1 -3 -4 -4 -2 -3 -3 -4 -4 4 1 -3 0 -1 -3 -3 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 43 0 0 0 0 0 0 0 0 0 57 0.58 0.18 11 R -2 5 -1 -2 -4 1 0 -3 -1 -4 -3 4 -2 -4 -2 -1 -1 -4 -2 -3 0 50 0 0 0 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 0.78 0.18 12 I -2 -2 2 3 -2 -2 -1 -3 -2 3 -1 -2 -1 -2 -3 -1 2 -4 -2 0 0 0 18 23 0 0 0 0 0 43 0 0 0 0 0 0 16 0 0 0 0.31 0.18 13 E -2 -1 3 3 -4 1 4 -2 5 -4 -4 0 -3 -3 -2 -1 -1 -4 -1 -4 0 0 16 16 0 0 44 0 23 0 0 0 0 0 0 0 0 0 0 0 0.65 0.18 14 T -2 -2 2 -2 -2 -2 -2 -3 -2 2 3 -2 0 -1 -3 -1 3 -3 -2 0 0 0 16 0 0 0 0 0 0 16 41 0 0 0 0 0 26 0 0 0 0.29 0.18 15 G 0 -3 -1 -2 -3 -2 -3 6 -3 -5 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.31 0.18 16 E 2 -1 -2 -1 -2 0 3 -2 -2 -1 1 2 -1 -2 -2 -1 -1 -3 -2 1 23 0 0 0 0 0 26 0 0 0 16 18 0 0 0 0 0 0 0 16 0.20 0.18 17 W -3 -3 -5 -5 -3 -3 -4 -3 -3 -3 -2 -4 -2 0 -4 -3 -3 12 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.75 0.18 18 E -1 0 -1 1 -4 4 5 -3 0 -4 -3 0 -2 -4 -2 -1 -1 -3 -3 -3 0 0 0 0 0 23 77 0 0 0 0 0 0 0 0 0 0 0 0 0 0.86 0.18 19 K -1 3 -1 -2 -3 0 -1 2 -2 -4 -3 3 -2 -4 3 1 -1 -4 -3 -3 0 18 0 0 0 0 0 23 0 0 0 26 0 0 16 16 0 0 0 0 0.47 0.18 20 G 0 -3 -1 -2 -3 -2 -3 6 -3 -5 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.31 0.18 21 W -3 -3 -5 -5 -3 -3 -4 -3 -3 -3 -2 -4 -2 0 -4 -3 -3 12 2 -4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.75 0.18 22 N -1 4 4 -1 -3 0 -1 -2 5 -4 -3 0 -2 -3 -2 1 -1 -4 -1 -3 0 34 26 0 0 0 0 0 23 0 0 0 0 0 0 16 0 0 0 0 0.55 0.18 23 V -2 -3 -4 -4 -2 -3 -4 -4 -4 4 3 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 33 41 0 0 0 0 0 0 0 0 26 0.51 0.18 24 L -2 -3 -4 -4 -2 -2 -3 -4 -3 1 4 -3 4 0 -3 -3 -1 -2 -2 2 0 0 0 0 0 0 0 0 0 0 59 0 23 0 0 0 0 0 0 18 0.53 0.18 25 V -1 -3 -3 -3 -1 -2 -3 -3 -3 2 2 -2 0 -1 -3 -1 2 -3 -2 4 0 0 0 0 0 0 0 0 0 0 18 0 0 0 0 0 23 0 0 59 0.40 0.18 26 W -2 -3 -3 -4 -2 -2 -3 -3 -3 -1 -1 -3 -1 0 -3 -2 1 10 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 59 0 23 1.38 0.18 27 G 0 -3 -1 -2 -3 -2 -3 6 -3 -5 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.31 0.18 28 R -2 7 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -4 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.07 0.18 29 G 0 -3 -1 -2 -3 -3 -3 6 -3 -5 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.34 0.20 30 Y -2 -2 -3 -4 -3 -2 -3 -4 1 -2 -2 -3 -2 3 -4 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.30 0.20 31 A 5 -2 -2 -2 -1 -1 -2 0 -2 -2 -2 -1 -2 -3 -1 1 -1 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.69 0.20 32 A 4 -2 -3 -3 -1 -2 -2 -1 -3 0 -1 -2 -1 -2 -2 0 -1 -3 -2 3 62 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 38 0.44 0.20 33 V -1 -2 -2 -2 -1 -2 -2 -2 -2 2 0 -2 0 -1 -2 -2 0 -2 -1 5 2 1 1 1 0 1 2 2 1 1 2 1 1 1 1 2 1 0 1 77 0.39 0.15 34 K -1 1 -1 -1 -3 1 0 -2 -1 -2 -2 5 -1 -3 -1 -1 -1 -2 -2 -2 2 1 1 1 0 1 2 2 1 1 2 77 1 1 1 2 1 0 1 2 0.53 0.15 35 N -1 0 4 0 -2 0 -1 -1 4 -2 -2 1 -2 -1 -2 0 -1 -1 3 -2 2 1 37 1 0 1 2 2 14 1 2 14 1 1 1 2 1 0 15 2 0.36 0.15 36 R 1 3 -1 -1 -3 0 1 3 4 -4 -3 2 -2 -3 -2 -1 -2 -3 -2 -3 14 22 0 0 0 0 12 24 14 0 0 14 0 0 0 0 0 0 0 0 0.39 0.20 37 D -2 -1 0 4 -4 4 4 -2 -1 -4 -4 0 -3 -4 -2 -1 -2 -4 -3 -3 0 0 0 34 0 24 42 0 0 0 0 0 0 0 0 0 0 0 0 0 0.77 0.20 38 T 0 -2 0 3 -2 -1 0 -1 -2 -3 -3 -1 -2 -3 -2 4 3 -4 -3 -2 0 0 0 24 0 0 0 0 0 0 0 0 0 0 0 54 22 0 0 0 0.53 0.20 39 D -1 -3 0 4 -4 -2 -1 5 -2 -4 -4 -2 -4 -4 -3 -1 -2 -4 -4 -4 0 0 0 34 0 0 0 66 0 0 0 0 0 0 0 0 0 0 0 0 0.94 0.20 40 K -1 2 4 -1 -3 0 -1 -2 -1 -3 -3 3 -2 -4 -2 0 2 -4 -3 -2 0 14 26 0 0 0 0 0 0 0 0 35 0 0 0 0 24 0 0 0 0.47 0.20 41 V -1 -3 -4 -4 -2 -3 -3 -4 -4 3 0 -3 0 2 -3 -3 -1 -3 -1 5 0 0 0 0 0 0 0 0 0 14 0 0 0 14 0 0 0 0 0 72 0.54 0.20 42 I -2 -1 -3 -3 -2 -2 -2 -4 -3 3 2 2 0 -1 -3 -2 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 36 26 24 0 0 0 0 0 0 0 14 0.33 0.20 43 W -3 -3 -4 -5 -3 -2 -3 -3 -3 -3 -2 -4 -2 1 -4 -3 -3 12 2 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 2.63 0.13 44 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.55 0.13 45 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.20 0.13 46 S 3 -1 0 -1 -1 -1 -1 0 -2 -2 -3 -1 -2 -3 -1 4 1 -3 -2 -1 39 0 0 0 0 0 0 0 0 0 0 0 0 0 0 61 0 0 0 0 0.48 0.13 47 R -2 7 -1 -2 -4 1 0 -3 -1 -4 -3 2 -2 -3 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.98 0.13 48 K -2 1 -1 -2 -3 0 0 -2 -1 -3 -3 5 -2 -2 -2 -1 -1 7 -1 -3 0 0 0 0 0 0 0 0 0 0 0 76 0 0 0 0 0 24 0 0 0.78 0.13 49 V -1 -3 -3 -4 -1 -3 -3 -4 -4 2 0 -3 0 -1 -3 -2 0 -3 -2 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.55 0.13 50 K -1 1 -1 -1 -3 1 0 -2 -1 1 -2 5 -1 -3 -2 -1 -1 -3 -2 -1 0 0 0 0 0 0 0 0 0 17 0 83 0 0 0 0 0 0 0 0 0.49 0.13 51 P -1 -3 -3 -2 -3 -2 -2 -3 -3 -3 -3 -1 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.20 0.13 52 D -2 -2 1 6 -4 0 3 -2 -1 -4 -4 -1 -3 -4 -2 -1 -1 -4 -3 -3 0 0 0 78 0 0 22 0 0 0 0 0 0 0 0 0 0 0 0 0 0.90 0.08 53 I -2 -3 -4 -4 -2 -3 -4 -4 -4 4 3 -3 1 0 -3 -3 -1 -3 -2 2 0 0 0 0 0 0 0 0 0 72 28 0 0 0 0 0 0 0 0 0 0.44 0.06 K Lambda Standard Ungapped 0.1388 0.3164 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1606 0.3182 PSI Gapped 0.0491 0.2670