Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.00 0.00 2 I -1 -3 -4 -4 -1 -3 -3 -4 -4 3 2 -3 1 -1 -3 -2 -1 -3 -2 4 0 0 0 0 0 0 0 0 0 18 15 0 0 0 0 0 0 0 0 66 0.47 0.12 3 R 0 4 -1 0 -4 1 4 -3 -1 -2 -3 2 -2 -3 -2 -1 -1 -4 -2 -1 6 36 0 0 0 0 41 0 0 0 0 8 0 0 0 0 0 0 0 9 0.51 0.19 4 F -3 -4 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 -1 8 -4 -3 -3 0 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.15 0.19 5 S -1 -2 -1 -2 -2 -2 -2 -2 -3 0 0 -2 -1 -2 -2 1 5 -3 -2 1 0 0 0 0 0 0 0 0 0 0 12 0 0 0 0 10 67 0 0 11 0.47 0.19 6 V 3 -3 -3 -3 -1 -2 -2 -2 -3 1 -1 -2 0 -2 -2 -1 -1 -3 -2 4 46 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 54 0.41 0.19 7 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.25 0.19 8 R -2 6 -1 -2 -4 2 0 -3 -1 -4 -3 2 -2 -4 -3 -1 -2 -3 -2 -3 0 88 0 0 0 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.97 0.19 9 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -4 8 -1 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.27 0.19 10 F -3 -4 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 -1 8 -4 -3 -3 0 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.15 0.19 11 H 1 3 -2 -2 2 -1 -2 0 3 -1 -1 -1 -1 -2 -2 0 0 -3 -2 1 15 23 0 0 6 0 0 6 10 0 6 0 0 0 0 7 7 0 0 20 0.16 0.19 12 I -3 -4 -4 -4 -3 -4 -4 -4 -2 1 0 -4 0 7 -4 -3 -2 0 2 0 0 0 0 0 0 0 0 0 0 10 0 0 0 83 0 0 0 0 0 7 0.90 0.19 13 V 1 -3 -3 -4 -1 -3 -3 -3 -4 2 0 -3 0 -2 -3 -2 1 -4 -2 5 12 0 0 0 0 0 0 0 0 9 0 0 0 0 0 0 7 0 0 73 0.46 0.19 14 V -2 -3 -4 -4 -2 -3 -4 -4 -4 1 5 -3 2 0 -4 -3 -2 -2 -2 1 0 0 0 0 0 0 0 0 0 0 90 0 0 0 0 0 0 0 0 10 0.66 0.19 15 R -2 7 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -4 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.09 0.19 16 R -2 7 -1 -2 -4 1 -1 -3 -1 -4 -3 2 -2 -4 -3 -1 -2 -3 -2 -3 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.09 0.19 17 R -1 5 -1 -2 -4 0 -1 3 -1 -4 -3 2 -2 -4 -3 -1 -2 -3 -3 -3 0 62 0 0 0 0 0 29 0 0 0 9 0 0 0 0 0 0 0 0 0.72 0.19 18 G 1 -2 -1 -1 2 -1 1 5 -2 -4 -4 -1 -3 -4 1 1 -1 -3 -3 -3 7 0 0 0 7 0 16 50 0 0 0 0 0 0 6 14 0 0 0 0 0.56 0.19 19 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -2 -3 -1 1 -1 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.68 0.19 20 I 2 -3 -3 -3 -2 -3 -3 -3 -3 4 1 -3 0 -1 -3 -1 -1 -3 -2 3 28 0 0 0 0 0 0 0 0 38 12 0 0 0 0 0 0 0 0 21 0.35 0.19 21 L -2 -3 -4 -4 -2 -3 -4 -4 -4 1 5 -3 2 0 -4 -3 -2 -2 -2 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.73 0.19 22 F -3 -4 -4 -4 -3 -4 -4 -4 -2 -1 0 -4 -1 8 -4 -3 -3 0 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 1.15 0.19 23 L 0 -2 -2 -3 -2 -2 -2 -3 -3 0 3 -2 2 -1 -3 -1 3 -3 -2 0 9 0 0 0 0 0 0 0 0 0 49 0 7 0 0 0 35 0 0 0 0.36 0.19 24 G 0 -3 -1 -2 -3 -2 -3 6 -3 -5 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.33 0.19 25 S 0 5 -1 -2 -3 0 -1 2 2 -3 -3 1 -2 -3 -3 0 -1 -3 -2 -1 6 54 0 0 0 0 0 16 7 0 0 0 0 0 0 10 0 0 0 7 0.46 0.19 26 I -1 -3 -4 -4 -2 -3 -3 -4 -4 4 1 -3 0 -1 -3 -3 -1 -4 -2 5 0 0 0 0 0 0 0 0 0 37 0 0 0 0 0 0 0 0 0 63 0.59 0.19 27 A 2 -2 -1 -2 -1 -1 -2 -2 -2 -1 -1 -1 -1 -3 -2 2 4 -3 -2 0 23 0 0 0 0 0 0 0 0 0 5 0 0 0 0 12 53 0 0 6 0.41 0.19 28 D 0 -2 1 6 -3 -1 2 -2 -2 -4 -4 -1 -3 -4 -2 1 -1 -5 -3 -3 6 0 0 72 0 0 9 0 0 0 0 0 0 0 0 13 0 0 0 0 0.84 0.19 29 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -4 8 -1 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.27 0.19 30 H 1 -1 -2 -1 -2 0 3 -3 3 0 -1 0 -1 -2 -2 -1 -1 -4 -2 3 11 0 0 0 0 0 27 0 10 0 0 7 0 0 0 0 0 0 0 45 0.26 0.19 31 D -2 -2 1 7 -4 -1 1 -2 -2 -4 -4 -1 -4 -4 -2 -1 -2 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.25 0.19 32 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -4 -4 -2 -3 -4 8 -1 -2 -5 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.27 0.19 33 G 0 -3 -1 -2 -3 -2 -3 6 -3 -5 -4 -2 -3 -4 -3 -1 -2 -3 -4 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.29 0.16 34 P -1 -3 -3 -2 -4 -2 -2 -3 -3 -3 -4 -2 -3 -4 8 -1 -2 -4 -4 -3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 2.24 0.16 35 A 5 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 -1 -3 -2 -1 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.64 0.16 K Lambda Standard Ungapped 0.1505 0.3314 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1667 0.3132 PSI Gapped 0.0510 0.2670