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Pretty Alignments via ALSCRIPT

DSTAMP generates input files for GJBs ALSCRIPT program. Given a STAMP alignment file, DSTAMP can be run to create a fairly pretty alignment. Detailed descriptions of the parameters are given below. As a quick example, using the globin example,

dstamp -f globin.5.clean -prefix globin_align

will create files:

globin_align_als.com
globin_align_als.bloc

The first is a set of ALSCRIPT commands and the second is a modified alignment file. To get a pretty Postscript alignment, one needs to run alscript:

alscript globin_align_als.com

The file globin_align_als.ps will be created, and is previewable or printable on a Postscript printer. And is shown in Figure 1.

By default, residues occuring within structurally equivalent regions will be bold-faced, boxed and in upper- case, with other regions being lower-case, non-bold and in a smaller font. All secondary structure assignments will be shown below. It is possible to modify the output format (paramaters are described in a Chapter 4).

Figure 1 Globin alignment as discussed in the text.


Rob Russell and Geoff Barton