My group works in the field of Bioinformatics with emphasis on the development and application of computational techniques to bridge the expanding information gap between protein sequence (of which around 80,000 are now known), three-dimensional structure (of which about 2,000 are known) and function. A variety of approaches have been developed and protein families studied. Some recent highlights are the detection of structural similarity between the HIV p17 matrix protein and gamma interferon (Matthews et al, 1994), and the SH2 domain and E. coli BirA (Russell &Barton, 1993). A study of the factors that stabilise protein cores has revealed striking differences between structurally similar proteins (Russell &Barton, 1994). Secondary structure predictions performed for tyrosine phosphatases, factor XIIIa and G6PDH before the 3D structure of these proteins were known have now been confirmed by X-ray crystallography (Livingstone &Barton, 1994). Finally, new sequence comparison techniques have revealed similarities between Ser/Thr phosphatases and diadenosine tetraphosphatase (Barton, 1993; Barton, et al, 1994). Projects currently in progress include, protein tertiary fold recognition from predicted secondary structure, protein protein docking, protein domain location, and secondary structure prediction by pattern matching. Further details of work in the group and paper preprints can be obtained on the World Wide Web URL=http://www.compbio.dundee.ac.uk.
Barton, G. J., Cohen, P. T. C. and Barford, D. (1994), "Conservation Analysis and Structure Prediction of the Protein Serine/Threonine Phosphatases: Diadenosine Tetra-phosphatase from E. coli is Homologous to the Protein Phosphatases", Eur. J. Biochem., 220, 225-237. Barton, G. J. (1993b), "An Efficient Algorithm to Locate All Locally Optimal Alignments Between Two Sequences Allowing for Gaps", Comp. Appl. Bio. Sci., 9, 729-734. Livingstone, C. D. and Barton, G. J. (1993), "Protein Sequence Alignments: A Strategy for the Hierarchical Analysis of Residue Conservation", Comp. Appl. Bio. Sci., 9, 745-756. Livingstone, C. D. and Barton, G. J. (1994), "Secondary Structure Prediction from Multiple Sequence Data: Blood Clotting Factor XIII and Yersinia Protein Tyrosine Phosphatase", Int. J. Pep. Prot. Res., 44, 239. Matthews, S., Barlow, P., Boyd, J., Barton, G. Russell, R., Mills, H., Cunningham, M., Meyers, N., Burns, N., Clark, N., Kingsman, S., Kingsman, A. and Campbell, I., (1994) "Structural similarity between the p17 matrix protein of HIV-1 and interferon-gamma", Nature, 370, 666-668. Russell, R. B. and Barton, G. J. (1994), "Structural features can be unconserved in proteins with similar folds: An analysis of side-chain to side-chain contacts, secondary structure and accessibility", J. Mol. Biol., In Press (Accepted 30 Aug 1994). Russell, R. B. and Barton, G. J. (1993), "An SH2-SH3 Domain hybrid", Nature, 364, 765. Russell, R. B. and Barton, G. J. (1993), "The Limits of Protein Secondary Structure Prediction Accuracy from Multiple Sequence Alignment", J. Mol. Biol., 234, 951-957.
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