SH2 domain analysis performed using the program AMAS . The aim of this analysis is to locate patterns of amino acid conservation within each sub-group of related sequences and across all sequences in the set. The sequences are clustered by their overall similarity, then a set-based method is used to find the positions that have conserved physico-chemical properties within each group and between pairs of groups. The conservation is summarised by colour coding the alignment (shown here as grey shading). Pair conservation is summarised as a histogram. The histogram helps to locate conservation patterns characteristic of - helix and - strand. For full details see . For details of how to obtain AMAS and other programs from the author's group please download the file README from www.compbio.dundee.ac.uk.