PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between POLR2H and POLR2A

The interaction score between POLR2H and POLR2A is 856.00.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between POLR2H and POLR2A = 0.49

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

4.62E-3

Orthology

Organism	POLR2H Orthologue		POLR2A Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
bakers yeast	YOR224C	1	YDL140C	1	Other AffinityCaptureWestern CoCrystalStructure Get References From PubMed
worm	Q19826	1	P16356	1
fly	Q9VNZ3	1	P04052	1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

0.593

Domains

No domains were used in calculating likelihood of interaction

0.016

Post-translational Modifications

Protein: POLR2H

PTM	Residue	Reference
N-acetylalanine	1	HPRD

Protein: POLR2A

PTM	Residue	Reference
Phosphorylation	1836	PubMed, HPRD
Phosphorylation	1878	PubMed, HPRD
Phosphorylation	1882	PubMed, HPRD
Phosphorylation	1896	PubMed, HPRD
Phosphorylation	1927	PubMed, HPRD
Phosphorylation	1929	PubMed, HPRD
Phosphorylation	1930	PubMed, HPRD
Phosphorylation	1934	PubMed, HPRD
Phosphoserine	1896	HPRD
Phosphoserine	1934	HPRD
Phosphotyrosine	145	HPRD

Localisation

Protein: POLR2H

Localisation	Reference
Cytosol	HPRD
Nucleus	HPRD
Nucleolus	HPRD
Nucleoplasm	HPRD

Protein: POLR2A

Localisation	Reference
Nucleus	HPRD
Nucleoplasm	HPRD

Transitive Score

The common interactors between POLR2H and POLR2A that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between POLR2H andPOLR2A. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , POLR2H has 295 interactors and POLR2A has 803 interactors of which there are 156 common interactors.

Common Interactor	Name of Common Interactor	Score for POLR2H-Interactor (?)	Score for POLR2A-Interactor (?)
POLR2G	POLR2G: DNA-directed RNA polymerase II subunit RPB7	19.70	2.95
POLR2B	POLR2B: DNA-directed RNA polymerase II subunit RPB2	1.82	0.657
POLR1C	POLR1C, POLR1E: DNA-directed RNA polymerases I and III subunit RPAC1	1.16	0.025
POLR2D	POLR2D: DNA-directed RNA polymerase II subunit RPB4	1.10	0.890
POLR2E	POLR2E: DNA-directed RNA polymerases I, II, and III subunit RPABC1	1.10	0.657
C1orf82	RPAP2, C1orf82: RNA polymerase II-associated protein 2	0.902	0.685
POLR2L	POLR2L: DNA-directed RNA polymerases I, II, and III subunit RPABC5	0.890	0.538
ELAVL1	ELAVL1, HUR: ELAV-like protein 1	0.822	0.139
KHDRBS1	KHDRBS1, SAM68: KH domain-containing, RNA-binding, signal transduction-associate	0.804	0.254
SUPT5H	SUPT5H: Uncharacterized protein SUPT5H	0.593	0.593
GTF2F2	GTF2F2: Uncharacterized protein GTF2F2	0.593	0.593
SETD2	SETD2, HIF1, HSPC069, HYPB, KIAA1732, SET2: Histone-lysine N-methyltransferase S	0.593	0.715
YARS	YARS: Tyrosyl-tRNA synthetase, cytoplasmic	0.549	0.139
POLR2C	POLR2C, A-152E5.7: DNA-directed RNA polymerase II subunit RPB3	0.538	0.326
FNBP1	N/A	0.433	0.029
POLR2I	POLR2I: DNA-directed RNA polymerase II subunit RPB9	0.354	0.354
EIF5AP1	EIF5A: Eukaryotic translation initiation factor 5A-1	0.287	0.155
SNRPD1	SNRPD1: Small nuclear ribonucleoprotein Sm D1	0.276	0.038
SNRPD3	SNRPD3: Small nuclear ribonucleoprotein Sm D3	0.276	0.046
EIF2S1	EIF2S1, EIF2A: Eukaryotic translation initiation factor 2 subunit 1	0.257	0.139
MCM3	MCM3: DNA replication licensing factor MCM3	0.257	0.114
EIF2S2	EIF2S2, EIF2B: Eukaryotic translation initiation factor 2 subunit 2	0.257	0.046
YBX1	YBX1, NSEP1, YB1: Nuclease sensitive element-binding protein 1	0.257	0.139
EIF2B5	EIF2B5, EIF2BE: Translation initiation factor eIF-2B subunit epsilon	0.257	0.114
TP53	TP53, P53: Cellular tumor antigen p53	0.241	0.038
PABPC1	PABPC1, PAB1, PABP1, PABPC2: Polyadenylate-binding protein 1	0.233	0.139
PABPN1	PABPN1, PAB2, PABP2: Polyadenylate-binding protein 2	0.233	0.139
HNRPH3	HNRPH3: Heterogeneous nuclear ribonucleoprotein H3	0.187	0.187
EWSR1	EWSR1, EWS: RNA-binding protein EWS	0.187	0.187
CHD4	CHD4: Chromodomain-helicase-DNA-binding protein 4	0.179	0.052
SNRPA	SNRPA: U1 small nuclear ribonucleoprotein A	0.160	0.027
EIF3S9	EIF3S9: Eukaryotic translation initiation factor 3 subunit 9	0.160	0.188
PABPC4	PABPC4, APP1, PABP4: Polyadenylate-binding protein 4	0.160	0.139
CDC6	CDC6, CDC18L: Cell division control protein 6 homolog	0.156	0.038
MDN1	MDN1, KIAA0301: Midasin	0.156	0.038
BLM	BLM, RECQ2, RECQL3: Bloom syndrome protein	0.156	0.038
MCM4	MCM4, CDC21: DNA replication licensing factor MCM4	0.156	0.114
ORC1L	ORC1L, ORC1, PARC1: Origin recognition complex subunit 1	0.156	0.029
MCM2	MCM2, BM28, CCNL1, CDCL1, KIAA0030: DNA replication licensing factor MCM2	0.156	0.114
PRKDC	PRKDC, HYRC, HYRC1: DNA-dependent protein kinase catalytic subunit	0.155	0.052
HMGA1	HMGA1, HMGIY: High mobility group protein HMG-I/HMG-Y	0.152	0.152
PCNA	PCNA: Proliferating cell nuclear antigen	0.129	0.038
WRN	WRN, RECQ3, RECQL2: Werner syndrome ATP-dependent helicase	0.126	0.038
RPA2	RPA2, REPA2, RPA32, RPA34: Replication protein A 32 kDa subunit	0.126	0.046
FXR1	FXR1: Fragile X mental retardation syndrome-related protein 1	0.123	0.0818
PSMC1	PSMC1: 26S protease regulatory subunit 4	0.123	0.032
RPA1	RPA1, REPA1, RPA70: Replication protein A 70 kDa DNA-binding subunit	0.123	2.95
HSF2	HSF2, HSTF2: Heat shock factor protein 2	0.114	0.038
BRCA2	BRCA2, FACD, FANCD1: Breast cancer type 2 susceptibility protein	0.104	0.077
EXOSC2	EXOSC2, RRP4: Exosome complex exonuclease RRP4	0.093	0.069
RBMX	RBMX, HNRPG, RBMXP1: Heterogeneous nuclear ribonucleoprotein G	0.084	0.127
DDX21	DDX21: Nucleolar RNA helicase 2	0.084	0.0425
SMARCA4	SMARCA4, BRG1, SNF2B, SNF2L4: Probable global transcription activator SNF2L4	0.084	0.070
RNPS1	RNPS1, LDC2: RNA-binding protein with serine-rich domain 1	0.084	0.210
H2AFX	H2AFX, H2AX: Histone H2A.x	0.078	0.038
LMNB1	LMNB1, LMN2, LMNB: Lamin-B1	0.078	0.029
KIAA0788	ASCC3L1, HELIC2, KIAA0788: U5 small nuclear ribonucleoprotein 200 kDa helicase	0.075	0.046
TIAL1	TIAL1: Nucleolysin TIAR	0.075	0.139
DDX41	DDX41, ABS: Probable ATP-dependent RNA helicase DDX41	0.075	0.046
SFRS4	SFRS4, SRP75: Splicing factor, arginine/serine-rich 4	0.075	0.114
SYNCRIP	SYNCRIP, HNRPQ, NSAP1: Heterogeneous nuclear ribonucleoprotein Q	0.075	6.04
SNRPB2	SNRPB2: U2 small nuclear ribonucleoprotein B''	0.075	0.114
SFRS2	SFRS2: Splicing factor, arginine/serine-rich 2	0.075	0.139
HNRPF	HNRPF: Heterogeneous nuclear ribonucleoprotein F	0.075	0.114
SSB	SSB: Lupus La protein	0.075	0.139
SFRS1	SFRS1, ASF, OK/SW-cl.3, SF2, SF2P33: Splicing factor, arginine/serine-rich 1	0.075	0.114
HNRPA2B1	HNRPA2B1: Heterogeneous nuclear ribonucleoproteins A2/B1	0.075	0.075
SUPT5H	SUPT5H, SPT5, SPT5H: Transcription elongation factor SPT5	0.0727	0.090
TAF10	TAF2A, TAF10, TAF2H, TAFII30: Transcription initiation factor TFIID subunit 10	0.063	0.063
K-ALPHA-1	TUBA1B: Tubulin alpha-1B chain	0.063	0.038
CSDA	CSDA, DBPA: DNA-binding protein A	0.058	0.038
SF1	SF1, ZFM1, ZNF162: Splicing factor 1	0.056	0.028
HMGN1	HMGN1, HMG14: Non-histone chromosomal protein HMG-14	0.054	0.052
SET	SET: Protein SET	0.054	0.0425
TRIM28	TRIM28, KAP1, RNF96, TIF1B: Transcription intermediary factor 1-beta	0.054	0.070
G3BP	G3BP1, G3BP: Ras GTPase-activating protein-binding protein 1	0.0525	0.067
NR3C1	NR3C1, GRL: Glucocorticoid receptor	0.052	0.032
HNRPA0	HNRPA0: Heterogeneous nuclear ribonucleoprotein A0	0.051	0.127
RDBP	RDBP, NELFE, RD: Negative elongation factor E	0.051	0.210
DDX46	DDX46, KIAA0801: Probable ATP-dependent RNA helicase DDX46	0.051	0.0425
RBM14	RBM14, SIP: RNA-binding protein 14	0.051	0.210
MATR3	MATR3, KIAA0723: Matrin-3	0.051	0.127
RAD51	RAD51, RAD51A, RECA: DNA repair protein RAD51 homolog 1	0.050	0.025
DHX8	DHX8, DDX8: ATP-dependent RNA helicase DHX8	0.050	0.075
CARHSP1	CARHSP1: Calcium-regulated heat stable protein 1	0.050	0.075
MSH6	MSH6, GTBP: DNA mismatch repair protein MSH6	0.048	0.046
SNRPC	SNRPC: U1 small nuclear ribonucleoprotein C	0.048	0.046
NOL1	N/A	0.048	0.046
CSNK2A1	CSNK2A1: CSNK2A1 protein	0.048	0.046
ANAPC5	ANAPC5, APC5: Anaphase-promoting complex subunit 5	0.048	0.046
SSRP1	SSRP1, FACT80: FACT complex subunit SSRP1	0.048	0.038
NPM1	NPM1, NPM: Nucleophosmin	0.048	0.038
STAT1	STAT1: Signal transducer and activator of transcription 1-alpha/beta	0.047	0.025
HIF1A	HIF1A, MOP1: Hypoxia-inducible factor 1 alpha	0.046	0.029
H3F3A	H3F3A, H3.3A, PP781, H3.3B, H3F3B, H3F3: Histone H3.3	0.046	0.038
U2AF1	U2AF1, U2AF35, U2AFBP: Splicing factor U2AF 35 kDa subunit	0.045	0.027
CCNB1	Cyclin B1	0.045	0.038
WBSCR1	EIF4H, KIAA0038, WBSCR1, WSCR1: Eukaryotic translation initiation factor 4H	0.045	0.114
SMARCA5	SMARCA5, SNF2H, WCRF135: SWI/SNF-related matrix-associated actin-dependent regul	0.045	0.038
SFRS10	SFRS10, TRA2B: Splicing factor, arginine/serine-rich 10	0.045	0.139
BTAF1	BTAF1, TAF172: TATA-binding protein-associated factor 172	0.045	0.038
RBM15	RBM15, OTT: Putative RNA-binding protein 15	0.045	0.114
RB1	RB1: Retinoblastoma-associated protein	0.044	0.052
RBM6	RBM6, DEF3: RNA-binding protein 6	0.041	0.155
E2F1	E2F1, RBBP3: Transcription factor E2F1	0.041	0.0425
IRF3	IRF3: Interferon regulatory factor 3	0.041	0.0425
RBM3	RBM3, RNPL: Putative RNA-binding protein 3	0.041	0.127
ELF1	ELF1: ETS-related transcription factor Elf-1	0.041	0.0425
ZNF638	ZNF638, NP220, ZFML: Zinc finger protein 638	0.041	0.127
ATR	ATR, FRP1: Serine/threonine-protein kinase ATR	0.039	0.046
CTDP1	CTDP1, FCP1: RNA polymerase II subunit A C-terminal domain phosphatase	0.038	0.114
GTF2E2	GTF2E2, TF2E2: Transcription initiation factor IIE subunit beta	0.038	0.657
HNRPR	HNRPR: Heterogeneous nuclear ribonucleoprotein R	0.038	2.95
SF3B4	SF3B4, SAP49: Splicing factor 3B subunit 4	0.037	0.188
SPEN	SPEN, KIAA0929, MINT, SHARP: Msx2-interacting protein	0.037	0.139
DHX16	DHX16, DBP2, DDX16, KIAA0577: Putative pre-mRNA-splicing factor ATP-dependent RN	0.037	0.063
PIP5K3	PIP5K3, KIAA0981, PIKFYVE: FYVE finger-containing phosphoinositide kinase	0.037	0.038
EIF3S4	EIF3S4: Eukaryotic translation initiation factor 3 subunit 4	0.037	0.114
SFRS6	SFRS6, SRP55: Splicing factor, arginine/serine-rich 6	0.037	0.114
GTF2F1	GTF2F1, RAP74: Transcription initiation factor IIF subunit alpha	0.037	0.046
SKIV2L	SKIV2L, XXbac-BCX116I9.5-001, XXbac-BPG116M5.1-001: Superkiller viralicidic acti	0.037	0.046
SNRP70	SNRP70, RNPU1Z, RPU1, U1AP1: U1 small nuclear ribonucleoprotein 70 kDa	0.037	0.114
ACIN1	ACIN1, ACINUS, KIAA0670: Apoptotic chromatin condensation inducer in the nucleus	0.037	0.114
RBM7		0.037	0.069
SAFB	SAFB, HAP, HET, SAFB1: Scaffold attachment factor B	0.037	0.139
CSTF2T	CSTF2T, KIAA0689: Cleavage stimulation factor 64 kDa subunit, tau variant	0.037	4.59
FXR2	FXR2, FMR1L2: Fragile X mental retardation syndrome-related protein 2	0.036	0.051
SAT	SAT1, SAT: Diamine acetyltransferase 1	0.032	0.026
SF3B1	SF3B1, SAP155: Splicing factor 3B subunit 1	0.032	0.038
GTF2B	GTF2B, TF2B, TFIIB: Transcription initiation factor IIB	0.032	7.66
GTF2F2	N/A	0.030	0.0425
CREBBP	CREBBP, CBP: CREB-binding protein	0.029	0.038
SNRPB	N/A	0.028	0.028
MITF	MITF: Microphthalmia-associated transcription factor	0.028	0.028
SMARCA3	HLTF, HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1: Helicase-like transcription factor	0.027	0.038
SFRS5	SFRS5, HRS, SRP40: Splicing factor, arginine/serine-rich 5	0.027	0.114
DDX3Y	DDX3Y, DBY: ATP-dependent RNA helicase DDX3Y	0.027	0.046
DDX3X	DDX3X, DBX, DDX3: ATP-dependent RNA helicase DDX3X	0.027	0.038
ERCC3	ERCC3, XPB, XPBC: TFIIH basal transcription factor complex helicase XPB subunit	0.027	0.046
DDX24	DDX24: ATP-dependent RNA helicase DDX24	0.027	0.038
RBMX2	RBMX2, CGI-79: RNA-binding motif protein, X-linked 2	0.027	0.114
RBM8A	RBM8A, RBM8, HSPC114, MDS014: RNA-binding protein 8A	0.027	0.114
DDX5	DDX5, G17P1, HELR, HLR1: Probable ATP-dependent RNA helicase DDX5	0.027	0.038
DRIM	UTP20, DRIM: Small subunit processome component 20 homolog	0.026	0.026
FANCA	FANCA, FAA, FACA, FANCH: Fanconi anemia group A protein	0.025	0.052
TCOF1	N/A	0.025	0.052
ABCF1	N/A	0.025	0.052
UBAP2	N/A	0.025	0.087
HMGN2	HMGN2, HMG17: Non-histone chromosomal protein HMG-17	0.025	0.052
GNB2L1	Lung cancer oncogene 7	0.025	0.0425
ESPL1	N/A	0.025	0.0425
UBAP2L	UBAP2L, KIAA0144, NICE4: Ubiquitin-associated protein 2-like	0.025	0.070
XRCC6	XRCC6, G22P1: ATP-dependent DNA helicase 2 subunit 1	0.025	0.070
PTMA	N/A	0.025	0.0425
DNMT1	DNMT1, AIM, DNMT: DNA	0.025	0.070
CDC25C	CDC25C: M-phase inducer phosphatase 3	0.025	0.025

0.299

Total Score

856.00