PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between ASF1B and HIRA

The interaction score between ASF1B and HIRA is 74.60.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between ASF1B and HIRA = 0.32

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

3.80E-3

Orthology

Organism	ASF1B Orthologue		HIRA Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
bakers yeast	YJL115W	1	YBL008W	1	Y2H Immunoprecipitation pullDown AffinityCaptureMS AffinityCaptureWestern ReconstitutedComplex Get References From PubMed
worm	Q17603	1	Q09589	1
worm	Q19326	0.719	Q09589	1
fly	Q9V464	1	O17468	1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

0.593

Domains

No domains were used in calculating likelihood of interaction

1.48E-3

Post-translational Modifications

Protein: ASF1B

PTM	Residue	Reference

Protein: HIRA

PTM	Residue	Reference
Phosphorylation	555	PubMed, HPRD
Phosphorylation	687	PubMed, HPRD

Localisation

Protein: ASF1B

Localisation	Reference
Nucleus	HPRD
Cytoplasm	HPRD

Protein: HIRA

Localisation	Reference
Nucleus	HPRD

Transitive Score

The common interactors between ASF1B and HIRA that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between ASF1B andHIRA. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , ASF1B has 45 interactors and HIRA has 1125 interactors of which there are 15 common interactors.

Common Interactor	Name of Common Interactor	Score for ASF1B-Interactor (?)	Score for HIRA-Interactor (?)
CHAF1B	CHAF1B, CAF1A, CAF1P60, MPHOSPH7, MPP7: Chromatin assembly factor 1 subunit B	3.89	0.029
RRM2	RRM2, RR2: Ribonucleoside-diphosphate reductase subunit M2	2.15	0.046
CSNK2A1	CSNK2A1: CSNK2A1 protein	1.10	0.046
H3F3A	H3F3A, H3.3A, PP781, H3.3B, H3F3B, H3F3: Histone H3.3	0.408	98.70
CHEK2	CHEK2, CHK2, RAD53: Serine/threonine-protein kinase Chk2	0.354	0.025
BRD3	BRD3, KIAA0043, RING3L: Bromodomain-containing protein 3	0.326	0.046
HIST1H4A	HIST1H4A, H4/A, HIST1H4B, H4/I, H4/G, HIST1H4D, H4/B, H4/J, HIST1H4F, HIST4H4, H	0.233	29.20
LOC440093	Histone H3-like	0.119	0.119
LOC644914	H3L-like histone (Fragment)	0.119	0.119
HIST1H3A	HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD,	0.082	9.09
BRD2	BRD2, KIAA9001, RING3: Bromodomain-containing protein 2	0.082	0.063
HIST2H3C	HIST2H3A, HIST2H3C, H3F2, H3FM: Histone H3.2	0.071	0.071
BRD4	N/A	0.071	0.025
ESPL1	N/A	0.049	0.0425
HIST3H3	HIST3H3, H3FT: Histone H3.1t	0.047	9.09

0.347

Total Score

74.60