PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between STX3A and VAMP3

The interaction score between STX3A and VAMP3 is 1.42E3.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between STX3A and VAMP3 = 0.40

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

4.62E-3

Orthology

Organism

STX3A Orthologue

VAMP3 Orthologue

Experiment

Accession

Accession

bakers yeast

0.527

0.403

AffinityCaptureWestern

Get References From PubMed

bakers yeast

YMR183C

0.527

YOR327C

0.403

PhenotypicEnhancement
AffinityCaptureWestern

Get References From PubMed

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

0.064

Domains

Domains present in STX3A and VAMP3:

Domains in STX3A

Domains in VAMP3

IPR000727	Target SNARE coiled-coil domain
IPR006011	Syntaxin, N-terminal
IPR006012	Syntaxin/epimorphin family

IPR001388

Synaptobrevin

The Chi square scores for co-occurrence of domains that are present in STX3A and VAMP3 are listed below.

Domain 1		Domain 2		Chi Square
IPR000727	Target SNARE coiled-coil domain	IPR001388	Synaptobrevin	1090.58
IPR006012	Syntaxin/epimorphin family	IPR001388	Synaptobrevin	761.571
IPR006011	Syntaxin, N-terminal	IPR001388	Synaptobrevin	732.41

The background colour of the table refers to:

Blue Domain is only present in STX3A
Yellow Domain is only present in VAMP3
Green Domain is present in both STX3A and VAMP3

0.217

Post-translational Modifications

Protein: STX3A

PTM	Residue	Reference

Protein: VAMP3

PTM	Residue	Reference
Acetylation	2	PubMed, HPRD

Localisation

Protein: STX3A

Localisation	Reference
Plasma membrane	HPRD

Protein: VAMP3

Localisation	Reference
Plasma membrane	HPRD

Transitive Score

The common interactors between STX3A and VAMP3 that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between STX3A andVAMP3. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , STX3A has 50 interactors and VAMP3 has 99 interactors of which there are 17 common interactors.

Common Interactor	Name of Common Interactor	Score for STX3A-Interactor (?)	Score for VAMP3-Interactor (?)
STX11	STX11: Syntaxin-11	17.40	13.90
STX1B2	STX1B, STX1B1, STX1B2: Syntaxin-1B	11.50	5.55
EPIM	STX2, EPIM, STX2A, STX2B, STX2C: Syntaxin-2	11.50	5.55
STX4A	STX4, STX4A: Syntaxin-4	10.50	10.30
SNAP23	SNAP23: Synaptosomal-associated protein 23	6.70	25.70
SNAP25	SNAP25, SNAP: Synaptosomal-associated protein 25	4.47	3.97
STXBP3	STXBP3: Syntaxin-binding protein 3	4.19	0.097
STXBP1	N/A	3.01	0.124
STX1A	STX1A, STX1: Syntaxin-1A	0.448	30.70
SEC22L1	SEC22B, SEC22L1: Vesicle-trafficking protein SEC22b	0.360	0.132
STXBP2	STXBP2, UNC18B: Syntaxin-binding protein 2	0.267	0.300
NAPB	CDNA FLJ39175 fis, clone OCBBF2003593, highly similar to BETA-SOLUBLE NSF ATTACH	0.261	0.064
SNAP29	SNAP29: Synaptosomal-associated protein 29	0.162	0.214
BET1	BET1: BET1 homolog	0.0308	0.330
STX5A	N/A	0.028	15.70
STX7	STX7: Syntaxin-7	0.028	38.00
STX6	STX6: Syntaxin-6	0.027	0.265

0.347

Total Score

1.42E3