PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between RFC4 and RUVBL2

The interaction score between RFC4 and RUVBL2 is 17.00.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between RFC4 and RUVBL2 = 0.64

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

7.78E-3

Orthology

Organism	RFC4 Orthologue		RUVBL2 Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
bakers yeast	YJR068W	1	YPL235W	1
worm	P53016	1	Q9GZH2	1
fly	Q9VX15	1	Q9V3K3	1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

2.23E-3

Domains

Domains present in RFC4 and RUVBL2:

Domains in RFC4

Domains in RUVBL2

IPR003593	AAA ATPase
IPR003959	AAA ATPase, central region
IPR008921	DNA polymerase III clamp loader subunit, C-terminal
IPR000862	RFC
IPR013748	Replication factor C

IPR003593	AAA ATPase
IPR010339	TIP49, C-terminal

The Chi square scores for co-occurrence of domains that are present in RFC4 and RUVBL2 are listed below.

Domain 1		Domain 2		Chi Square
IPR003959	AAA ATPase, central region	IPR003593	AAA ATPase	133.351
IPR000862	RFC	IPR003593	AAA ATPase	128.851
IPR003593	AAA ATPase	IPR003593	AAA ATPase	89.0695

The background colour of the table refers to:

Blue Domain is only present in RFC4
Yellow Domain is only present in RUVBL2
Green Domain is present in both RFC4 and RUVBL2

0.027

Post-translational Modifications

No PTMs were used in calculating likelihood of interaction

Localisation

Protein: RFC4

Localisation	Reference
Nucleoplasm	HPRD

Protein: RUVBL2

Localisation	Reference
Cytosol	HPRD
Nucleus	HPRD
Nucleolus	HPRD
Nucleoplasm	HPRD
Cytoplasm	HPRD

Transitive Score

The common interactors between RFC4 and RUVBL2 that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between RFC4 andRUVBL2. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , RFC4 has 123 interactors and RUVBL2 has 182 interactors of which there are 62 common interactors.

Common Interactor	Name of Common Interactor	Score for RFC4-Interactor (?)	Score for RUVBL2-Interactor (?)
RFC3	RFC3: Replication factor C subunit 3	1.05E4	0.060
RFC5	RFC5: Replication factor C subunit 5	691.00	0.060
PCNA	PCNA: Proliferating cell nuclear antigen	54.70	0.047
KPNA6	N/A	2.37	0.408
KPNA5	KPNA5: Importin subunit alpha-6	1.18	0.408
KPNA1	KPNA1, RCH2: Importin subunit alpha-1	0.647	0.082
MCM6	MCM6: DNA replication licensing factor MCM6	0.620	0.047
ABCE1	ABCE1, RLI, OK/SW-cl.40, RNASEL1, RNASELI, RNS4I: ATP-binding cassette sub-famil	0.263	0.044
LAMR1	RPSA, LAMBR, LAMR1: 40S ribosomal protein SA	0.221	0.657
ORC6L	ORC6L: Origin recognition complex subunit 6	0.207	0.035
MCM2	MCM2, BM28, CCNL1, CDCL1, KIAA0030: DNA replication licensing factor MCM2	0.207	0.035
EIF2S1	EIF2S1, EIF2A: Eukaryotic translation initiation factor 2 subunit 1	0.207	0.047
MCM3	MCM3: DNA replication licensing factor MCM3	0.207	0.047
MCM4	MCM4, CDC21: DNA replication licensing factor MCM4	0.207	0.047
CDC6	CDC6, CDC18L: Cell division control protein 6 homolog	0.207	0.035
MCM5	MCM5, CDC46: DNA replication licensing factor MCM5	0.207	0.035
MCM7	MCM7, CDC47, MCM2: DNA replication licensing factor MCM7	0.207	0.047
KARS	KARS, KIAA0070: Lysyl-tRNA synthetase	0.137	0.083
TAF9	AK6, AD-004, CGI-137: Adenylate kinase isoenzyme 6	0.137	0.067
YBX1	YBX1, NSEP1, YB1: Nuclease sensitive element-binding protein 1	0.123	0.123
ATAD2	ATAD2, L16, PRO2000: ATPase family AAA domain-containing protein 2	0.123	0.060
RUVBL1	RUVBL1, NMP238, TIP49, TIP49A: RuvB-like 1	0.123	100.00
TRIP13	TRIP13: Thyroid receptor-interacting protein 13	0.123	0.123
SSBP1	SSBP1, SSBP: Single-stranded DNA-binding protein, mitochondrial precursor	0.117	0.060
DHX15	DHX15, DBP1, DDX15: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase	0.108	0.039
RPA1	RPA1, REPA1, RPA70: Replication protein A 70 kDa DNA-binding subunit	0.0968	0.035
POLR2H	POLR2H: DNA-directed RNA polymerases I, II, and III subunit RPABC3	0.0968	0.059
YARS	YARS: Tyrosyl-tRNA synthetase, cytoplasmic	0.0968	0.060
ORC1L	ORC1L, ORC1, PARC1: Origin recognition complex subunit 1	0.0968	0.035
BRCA2	BRCA2, FACD, FANCD1: Breast cancer type 2 susceptibility protein	0.083	0.0308
PSMC2	PSMC2, MSS1: 26S protease regulatory subunit 7	0.074	0.036
FXR1	FXR1: Fragile X mental retardation syndrome-related protein 1	0.074	0.074
SRP54	SRP54: Signal recognition particle 54 kDa protein	0.074	0.044
PSMC1	PSMC1: 26S protease regulatory subunit 4	0.074	0.060
PSMC6	PSMC6, SUG2: 26S protease regulatory subunit S10B	0.074	0.036
ATAD3A	ATAD3A: ATPase family AAA domain-containing protein 3A	0.074	0.044
SCYE1	SCYE1, EMAP2: Multisynthetase complex auxiliary component p43 [Contains: Endothe	0.074	0.036
DARS	DARS, PIG40: Aspartyl-tRNA synthetase, cytoplasmic	0.074	0.036
PSMC3	PSMC3, TBP1: 26S protease regulatory subunit 6A	0.074	0.044
EIF5AP1	EIF5A: Eukaryotic translation initiation factor 5A-1	0.067	0.067
EXOSC2	EXOSC2, RRP4: Exosome complex exonuclease RRP4	0.060	0.028
MDN1	MDN1, KIAA0301: Midasin	0.060	0.036
NARS	NARS, ASNS: Asparaginyl-tRNA synthetase, cytoplasmic	0.060	0.028
EIF1AX	EIF1AX, EIF1A, EIF4C: Eukaryotic translation initiation factor 1A, X-chromosomal	0.060	0.036
ORC2L	ORC2L, ORC2: Origin recognition complex subunit 2	0.059	0.047
EIF2B5	EIF2B5, EIF2BE: Translation initiation factor eIF-2B subunit epsilon	0.059	0.029
ORC3L	ORC3L, LATHEO: Origin recognition complex subunit 3	0.053	0.039
ASCC3	ASCC3, HELIC1: Activating signal cointegrator 1 complex subunit 3	0.047	0.029
POLR2G	POLR2G: DNA-directed RNA polymerase II subunit RPB7	0.047	0.035
VCP	VCP: Transitional endoplasmic reticulum ATPase	0.044	0.044
BCS1L	BCS1L, BCS1: Mitochondrial chaperone BCS1	0.044	0.036
SRPR	SRPR: Uncharacterized protein SRPR	0.041	0.0308
VPS4A	VPS4A, VPS4: Vacuolar protein sorting-associating protein 4A	0.036	0.028
NUBP1	NUBP1, NBP, NBP1: Nucleotide-binding protein 1	0.036	0.036
XAB1	XAB1, MBDIN, HUSSY-23: XPA-binding protein 1	0.0331	0.060
PRSS15	LONP1, PRSS15: Lon protease homolog, mitochondrial precursor	0.027	0.044
AFG3L2	AFG3L2: AFG3-like protein 2	0.027	0.044
CLPX	CLPX: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial pr	0.027	0.044
RPA3	RPA3, REPA3, RPA14: Replication protein A 14 kDa subunit	0.027	0.027
RAD51L1	RAD51L1, RAD51B, REC2: DNA repair protein RAD51 homolog 2	0.027	0.027
DNAH11	N/A	0.027	0.027
RAD51L3	RAD51L3, RAD51D: DNA repair protein RAD51 homolog 4	0.027	0.027

0.572

Total Score

17.00