PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between GNA14 and CHRM2

The interaction score between GNA14 and CHRM2 is 293.00.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between GNA14 and CHRM2 = 0.08

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

1.85E-3

Orthology

Organism	GNA14 Orthologue		CHRM2 Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
human	IPI00220578	0.162	IPI00023456	0.075
human	IPI00337415	0.162	IPI00023456	0.075
human	IPI00288947	0.054	IPI00023456	0.075	Other InVivo Get References From PubMed
worm	Q8T3G5	0.054	Q9U7D5	0.075

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

1.07

Domains

Domains present in GNA14 and CHRM2:

Domains in GNA14

Domains in CHRM2

IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit
IPR011025	Transducin, alpha subunit, insertion
IPR000654	G-protein alpha subunit, group Q

IPR000276	Rhodopsin-like GPCR superfamily
IPR000995	Muscarinic acetylcholine receptor
IPR001065	Muscarinic acetylcholine receptor M2

The Chi square scores for co-occurrence of domains that are present in GNA14 and CHRM2 are listed below.

Domain 1		Domain 2		Chi Square
IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	IPR000276	Rhodopsin-like GPCR superfamily	1146.41
IPR011025	Transducin, alpha subunit, insertion	IPR000276	Rhodopsin-like GPCR superfamily	1012.26
IPR000654	G-protein alpha subunit, group Q	IPR000276	Rhodopsin-like GPCR superfamily	560.059
IPR000654	G-protein alpha subunit, group Q	IPR001065	Muscarinic acetylcholine receptor M2	243.448
IPR000654	G-protein alpha subunit, group Q	IPR000995	Muscarinic acetylcholine receptor	214.497
IPR011025	Transducin, alpha subunit, insertion	IPR000995	Muscarinic acetylcholine receptor	104.457
IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	IPR000995	Muscarinic acetylcholine receptor	85.3953
IPR011025	Transducin, alpha subunit, insertion	IPR001065	Muscarinic acetylcholine receptor M2	71.9722
IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	IPR001065	Muscarinic acetylcholine receptor M2	59.0326

The background colour of the table refers to:

Blue Domain is only present in GNA14
Yellow Domain is only present in CHRM2
Green Domain is present in both GNA14 and CHRM2

1.05

Post-translational Modifications

Protein: GNA14

PTM	Residue	Reference
ADP-ribosylarginine	179	PubMed, HPRD

Protein: CHRM2

PTM	Residue	Reference
Glycosylation	2	PubMed, HPRD
Glycosylation	3	PubMed, HPRD
Glycosylation	6	PubMed, HPRD

Localisation

Protein: GNA14

Localisation	Reference
Plasma membrane	HPRD

Protein: CHRM2

Localisation	Reference
Plasma membrane	HPRD
Endosome	HPRD

Transitive Score

The common interactors between GNA14 and CHRM2 that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between GNA14 andCHRM2. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , GNA14 has 813 interactors and CHRM2 has 406 interactors of which there are 76 common interactors.

Common Interactor	Name of Common Interactor	Score for GNA14-Interactor (?)	Score for CHRM2-Interactor (?)
HTR2C	HTR2C, HTR1C: 5-hydroxytryptamine receptor 2C	418.00	0.136
HTR2A	HTR2A, HTR2: 5-hydroxytryptamine receptor 2A	105.00	0.028
GNA11	GNA11, GA11: Guanine nucleotide-binding protein subunit alpha-11	51.30	518.00
GNAI1	GNAI1: Guanine nucleotide-binding protein G(i), alpha-1 subunit	38.90	518.00
GNAO1	GNAO1: Guanine nucleotide-binding protein G(o) subunit alpha 1	33.70	1.17
GNAT1	GNAT1, GNATR: Guanine nucleotide-binding protein G(t), alpha-1 subunit	31.00	0.874
GNAZ	GNAZ: Guanine nucleotide-binding protein G(z) subunit alpha	31.00	1.07
GNAI3	GNAI3: Guanine nucleotide-binding protein G(k) subunit alpha	19.90	298.00
GNAT2	GNAT2, GNATC: Guanine nucleotide-binding protein G(t), alpha-2 subunit	19.90	1.07
FLT1	FLT1, FLT, FRT: Vascular endothelial growth factor receptor 1 precursor	4.65	0.029
OPRM1	OPRM1, MOR1: Mu-type opioid receptor	1.37	0.030
EDNRB	EDNRB, ETRB: Endothelin B receptor precursor	1.30	0.083
C3AR1	C3AR1, AZ3B, C3R1, HNFAG09: C3a anaphylatoxin chemotactic receptor	1.30	0.083
CCR2	CCR2, CMKBR2: C-C chemokine receptor type 2	1.30	0.083
OPRD1	OPRD1, OPRD: Delta-type opioid receptor	1.26	0.028
AVPR1A	AVPR1A, AVPR1: Vasopressin V1a receptor	1.26	0.046
EDG1	EDG1: Sphingosine 1-phosphate receptor Edg-1	1.21	0.055
F2R	F2R, CF2R, PAR1, TR: Proteinase-activated receptor 1 precursor	1.21	0.062
DRD1	DRD1: D(1A) dopamine receptor	1.07	0.069
TSHR	N/A	1.07	0.049
PTAFR	PTAFR, PAFR: Platelet-activating factor receptor	1.05	0.083
DRD2	DRD2: D(2) dopamine receptor	1.05	0.083
TBXA2R	TBXA2R: Thromboxane A2 receptor	1.05	0.083
RGS7	RGS7: Regulator of G-protein signaling 7	1.02	0.042
AVPR1B	AVPR1B, AVPR3, VPR3: Vasopressin V1b receptor	1.02	0.028
RHO	RHO, OPN2: Rhodopsin	0.975	0.077
AGTR1	AGTR1, AGTR1A, AGTR1B, AT2R1, AT2R1B: Type-1 angiotensin II receptor	0.970	0.077
F2RL1	F2RL1, GPR11, PAR2: Proteinase-activated receptor 2 precursor	0.970	0.062
HTR6	HTR6: 5-hydroxytryptamine receptor 6	0.970	0.096
P2RY4	P2RY4, NRU: P2Y purinoceptor 4	0.970	0.127
GPR23	LPA4, GPR23, P2RY9: Lysophosphatidic acid receptor 4	0.970	0.062
MC4R	MC4R: Melanocortin receptor 4	0.970	0.127
RGS4	RGS4: Regulator of G-protein signaling 4	0.902	0.081
PTGIR	PTGIR, PRIPR: Prostacyclin receptor	0.874	0.050
BDKRB2	BDKRB2, BKR2: B2 bradykinin receptor	0.821	0.031
F2RL2	F2RL2, PAR3: Proteinase-activated receptor 3 precursor	0.786	0.062
CXCR4	CXCR4: C-X-C chemokine receptor type 4	0.775	0.056
C5AR1	C5AR1, C5AR, C5R1: C5a anaphylatoxin chemotactic receptor	0.775	0.049
CCR5	CCR5: CCR5 receptor (Fragment)	0.775	0.055
ADRB2	ADRB2, ADRB2R, B2AR: Beta-2 adrenergic receptor	0.770	0.044
RGS16	RGS16, RGSR: Regulator of G-protein signaling 16	0.731	0.100
SSTR3	SSTR3: Somatostatin receptor type 3	0.704	0.056
TACR2	TACR2, NK2R, NKNAR, TAC2R: Substance-K receptor	0.704	0.069
F2RL3	F2RL3, PAR4: Proteinase-activated receptor 4 precursor	0.692	0.096
GNAQ	GNAQ, GAQ: Guanine nucleotide-binding protein G(q) subunit alpha	0.658	0.874
LTB4R	LTB4R, BLT, BLT1, BLTR, CMKRL1, GPR16, P2RY7: Leukotriene B4 receptor 1	0.624	0.056
FPR1	FPR1: fMet-Leu-Phe receptor	0.624	0.049
IL8RB	IL8RB, CXCR2: High affinity interleukin-8 receptor B	0.624	0.062
FPRL1	FPRL1, FPR2, FPRH1, LXA4R: FMLP-related receptor I	0.624	0.062
TRHR	TRHR: Thyrotropin-releasing hormone receptor	0.559	0.069
RGS18	RGS18, RGS13: Regulator of G-protein signaling 18	0.543	0.044
AXIN1		0.543	0.109
GNAI2	Galphai2 protein	0.469	0.693
GRK5	GRK5, GPRK5: G protein-coupled receptor kinase 5	0.448	31.30
GRK1	GRK1, RHOK: Rhodopsin kinase precursor	0.357	0.081
RGS2	RGS2, G0S8: Regulator of G-protein signaling 2	0.322	0.0727
ADRBK1	ADRBK1, BARK, BARK1, GRK2: Beta-adrenergic receptor kinase 1	0.320	0.064
RGS19	RGS19, GAIP, GNAI3IP: Regulator of G-protein signaling 19	0.288	0.065
ADORA3	ADORA3, UNQ1931/PRO4406: Adenosine A3 receptor	0.265	0.027
HTR1F	HTR1F, HTR1EL: 5-hydroxytryptamine receptor 1F	0.265	0.027
GRK7	GRK7, GPRK7: G protein-coupled receptor kinase 7 precursor	0.217	0.044
GRK4	GPRK2L, GRK4, GPRK4: G protein-coupled receptor kinase 4	0.195	9.04
GRIK2	GRIK2, GLUR6: Glutamate receptor, ionotropic kainate 2 precursor	0.097	0.054
CASR	CASR, GPRC2A, PCAR1: Extracellular calcium-sensing receptor precursor	0.085	0.032
GP1BA	N/A	0.077	0.026
GPRC5A	GPRC5A, GPCR5A, RAI3, RAIG1: Retinoic acid-induced protein 3	0.056	0.028
GRIA3	GRIA3, GLUR3, GLURC: Glutamate receptor 3 precursor	0.050	0.047
GRIA4	GRIA4, GLUR4: Glutamate receptor 4 precursor	0.046	0.054
GRIN1	GRIN1, NMDAR1: Glutamate [NMDA] receptor subunit zeta-1 precursor	0.037	0.035
GRIA1	GRIA1, GLUH1, GLUR1: Glutamate receptor 1 precursor	0.033	0.054
SNAP25	N/A	0.028	0.028
LAT	LAT: Linker for activation of T-cells family member 1	0.028	0.028
GNAO1	GNAO1: Guanine nucleotide-binding protein G(o) subunit alpha 2	0.026	1.20
TGFA	TGFA: Protransforming growth factor alpha precursor [Contains: Transforming grow	0.026	0.032
SNAP23	SNAP23: Synaptosomal-associated protein 23	0.025	0.025
STOM	STOM, BND7, EPB72: Erythrocyte band 7 integral membrane protein	0.025	0.025

2.15E-3

Total Score

293.00