PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between RFX1 and RFX2

The interaction score between RFX1 and RFX2 is 276.00.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

N/A

Orthology

Organism	RFX1 Orthologue		RFX2 Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
human	IPI00298182	0.468	IPI00102829	0.594	InVivo Get References From PubMed
human	IPI00102829	0.275	IPI00102829	0.594	InVitro Get References From PubMed
human	IPI00298182	0.468	IPI00419901	0.131	Other Y2H InVivo InVitro Get References From PubMed
human	IPI00102829	0.275	IPI00419901	0.131	InVitro InVivo Get References From PubMed
human	IPI00298182	0.468	IPI00219885	0.108	Other Y2H Get References From PubMed
human	IPI00102829	0.275	IPI00219885	0.108	Y2H Get References From PubMed
bakers yeast	YLR176C	0.468	YLR176C	1	pullDown Get References From PubMed
worm	Q09555	1	Q09555	0.242
fly	Q9VGZ6	0.268	Q9VGZ6	0.383

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

3.01

Domains

Domains present in RFX1 and RFX2:

Domains in RFX1

Domains in RFX2

IPR002173	Carbohydrate kinase, PfkB
IPR003150	DNA-binding RFX
IPR007668	RFX1 transcription activation region
IPR011991	Wing_hlx_DNA_bd.

IPR003150	DNA-binding RFX
IPR007668	RFX1 transcription activation region
IPR011991	Wing_hlx_DNA_bd.

The Chi square scores for co-occurrence of domains that are present in RFX1 and RFX2 are listed below.

Domain 1		Domain 2		Chi Square
IPR003150	DNA-binding RFX	IPR007668	RFX1 transcription activation region	3324.75
IPR003150	DNA-binding RFX	IPR003150	DNA-binding RFX	2415.75
IPR007668	RFX1 transcription activation region	IPR007668	RFX1 transcription activation region	1849.83
IPR007668	RFX1 transcription activation region	IPR011991	Wing_hlx_DNA_bd.	139.403
IPR003150	DNA-binding RFX	IPR011991	Wing_hlx_DNA_bd.	98.4697
IPR011991	Wing_hlx_DNA_bd.	IPR011991	Wing_hlx_DNA_bd.	27.7932

The background colour of the table refers to:

Blue Domain is only present in RFX1
Yellow Domain is only present in RFX2
Green Domain is present in both RFX1 and RFX2

0.265

Post-translational Modifications

Protein: RFX1

PTM	Residue	Reference
Phosphorylation	120	PubMed, HPRD
Phosphoserine	120	HPRD

Protein: RFX2

PTM	Residue	Reference

Localisation

Protein: RFX1

Localisation	Reference
Nucleus	HPRD

Protein: RFX2

Localisation	Reference
Nucleus	HPRD

Transitive Score

The common interactors between RFX1 and RFX2 that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between RFX1 andRFX2. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , RFX1 has 523 interactors and RFX2 has 91 interactors of which there are 57 common interactors.

Common Interactor	Name of Common Interactor	Score for RFX1-Interactor (?)	Score for RFX2-Interactor (?)
RFX3	RFX3: Transcription factor RFX3	6.22	12.60
BTAF1	BTAF1, TAF172: TATA-binding protein-associated factor 172	2.61	1.94
SMARCA5	SMARCA5, SNF2H, WCRF135: SWI/SNF-related matrix-associated actin-dependent regul	1.63	1.28
SMARCA2	SMARCA2, BRM, SNF2A, SNF2L2: Probable global transcription activator SNF2L2	1.33	0.354
HIST1H4A	HIST1H4A, H4/A, HIST1H4B, H4/I, H4/G, HIST1H4D, H4/B, H4/J, HIST1H4F, HIST4H4, H	1.22	0.354
RFX4	RFX4: Regulatory factor X4, isoform c	1.11	1.37
SMARCA1	SMARCA1, SNF2L, SNF2L1: Probable global transcription activator SNF2L1	0.997	0.257
TAF6	TAF6, TAF2E, TAFII70: Transcription initiation factor TFIID subunit 6	0.995	0.354
UTX	UTX: Ubiquitously transcribed X chromosome tetratricopeptide repeat protein	0.877	0.354
TAF1L	TAF1L: Transcription initiation factor TFIID 210 kDa subunit	0.682	1.27
YWHAE	YWHAE: 14-3-3 protein epsilon	0.449	0.196
SMARCC2	SMARCC2, BAF170: SWI/SNF-related matrix-associated actin-dependent regulator of	0.390	1.94
TAF1	TAF1, BA2R, CCG1, CCGS, TAF2A: Transcription initiation factor TFIID subunit 1	0.191	0.027
RFX5	RFX5: DNA-binding protein RFX5	0.174	0.265
FOXM1	FOXM1, FKHL16, HFH11, MPP2, WIN: Forkhead box protein M1	0.165	0.027
NFATC3	NFATC3, NFAT4: Nuclear factor of activated T-cells, cytoplasmic 3	0.165	0.027
NFATC2	NFATC2, NFAT1, NFATP: Nuclear factor of activated T-cells, cytoplasmic 2	0.165	0.027
ELF2	ELF2, NERF: ETS-related transcription factor Elf-2	0.165	0.027
HTATIP	HTATIP, TIP60: Histone acetyltransferase HTATIP	0.165	0.027
GTF2F2	N/A	0.152	0.027
ETV1	ETV1, ER81: ETS translocation variant 1	0.123	0.027
FOXJ2	FOXJ2, FHX: Forkhead box protein J2	0.123	0.027
E2F1	E2F1, RBBP3: Transcription factor E2F1	0.123	0.027
DDIT3	DDIT3, CHOP, GADD153: DNA damage-inducible transcript 3	0.123	0.027
SMARCA4	SMARCA4: SMARCA4 isoform 2	0.119	0.593
ERF	ERF: ETS domain-containing transcription factor ERF	0.109	0.027
NFATC1	N/A	0.109	0.027
BATF	BATF: Basic leucine zipper transcriptional factor ATF-like	0.109	0.027
CEBPA	CEBPA: CCAAT/enhancer-binding protein alpha	0.109	0.027
BAZ1B	BAZ1B, WBSC10, WBSCR10, WBSCR9, WSTF: Bromodomain adjacent to zinc finger domain	0.109	0.027
IRF3	IRF3: Interferon regulatory factor 3	0.109	0.027
BACH1	BACH1: Transcription regulator protein BACH1	0.109	0.027
SMARCA4	SMARCA4, BRG1, SNF2B, SNF2L4: Probable global transcription activator SNF2L4	0.109	0.027
TRIM28	TRIM28, KAP1, RNF96, TIF1B: Transcription intermediary factor 1-beta	0.109	0.027
RB1	RB1: Retinoblastoma-associated protein	0.109	0.027
ERG	ERG: Transcriptional regulator ERG	0.109	0.027
ETS2	ETS2: Protein C-ets-2	0.109	0.027
ELF1	ELF1: ETS-related transcription factor Elf-1	0.098	0.027
H1FX	H1FX: Histone H1x	0.098	0.027
TAF1	N/A	0.071	0.354
UTY	N/A	0.064	0.119
TTF1	N/A	0.064	0.119
SMARCC1	N/A	0.064	0.119
NRL	NRL: Neural retina-specific leucine zipper protein	0.041	0.027
RFXDC1	RFXDC1: Regulatory factor X domain containing 1	0.038	0.071
ESR1	N/A	0.0288	0.0288
CREM	hCREM-1: HCREM 1alpha protein	0.027	0.027
CREM	CREM, RP11-324I22.1-005: cAMP responsive element modulator	0.027	0.027
HSFY1	HSFY1, HSF2L, HSFY: Heat shock transcription factor, Y-linked	0.027	0.027
HILS1	HILS1: Spermatid-specific linker histone H1-like protein	0.027	0.027
SPIC	SPIC: Transcription factor Spi-C	0.027	0.027
C20orf116	C20orf116: Uncharacterized protein C20orf116 precursor	0.027	0.027
NFAT5	NFAT5, KIAA0827, TONEBP: Nuclear factor of activated T-cells 5	0.027	0.027
IRF7	IRF7: Interferon regulatory factor 7	0.027	0.027
FOXQ1	FOXQ1, HFH1: Forkhead box protein Q1	0.027	0.027
BRWD1	N/A	0.027	0.027
PAX3	PAX3, HUP2: Paired box protein Pax-3	0.027	0.027

2.15E-3

Total Score

276.00